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Search Results: 1 - 10 of 4644 matches for " Zuraida Ab Rahman "
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Establishment and Optimization Growth of Shoot Buds-Derived Callus and Suspension Cell Cultures of Kaempferia parviflora  [PDF]
Ab Rahman Zuraida, Othman Ayu Nazreena, Kamarulzaman Fatin Liyana Izzati, Ahmad Aziz
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.518284
Abstract:

Callus and suspension cells culture of Kaempferia parviflora was successfully established. Meristematic shoots can be used for utilization of plant cell biosynthetic capabilities for obtaining useful products from valuable medicinal plant to meet out the pharmaceutical demand and also for studying the metabolism. The medium containing combination of 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.2 mg/L napthyleneacetic acid (NAA) promoted the highest callus induction at 20%. Transferring the initiated callus on the medium with 1 mg/L 2,4-D enhanced the proliferation rate up to maximum fresh weight of 6.71 gm. Growth curve of cultured cells revealed that the cells continued to grow until 50 days of culture and showed the highest peak (fresh weight) at 40 days in all different initial weight tested ( 0.2, 0.5 and 1.0 gram). Isolated embryogenic callus was found to produce the highest in weight when suspended in liquid medium supplemented with 1 mg/L 2,4-D at 110 rpm resulted 13.5 gram fresh weight and 1080 mg dry weight.

Direct Shoot Regeneration from Callus of Melicope lunu-ankenda  [PDF]
Ab. Zuraida Rahman, Ayu Nazreena Othman, Fatin Liyana Izzati Kamaruddin, Aziz Bin Ahmad
Natural Science (NS) , 2015, DOI: 10.4236/ns.2015.72009
Abstract: Melicope lunu-ankenda is commonly used in traditional medicine. The conventional propagation method for this species is inefficient due to low propagation rate and its lengthy period to maturity. In addition, insufficient planting materials often pose a problem for the plantation sector. The tissue culture technique is best alternative to overcome the problems. The callus induction and direct shoot regeneration protocols for M. lunu-ankenda were established. Callus was successfully initiated from leaves explants cultured in MS medium added with 2,4-D at concentrations 0.5 to 5.0 mg/L singly or in combination with NAA at concentrations 1.0 to 10 mg/L. Shoot was regenerated from callus in phytohormone-free medium, BAP at concentrations 0.5 - 5.0 mg/L singly or in combination of BAP with NAA or 2,4-D at concentration 0.5 and 1.0 mg/L, respectively. BAP at 1.0 mg/L induced the highest shoot regeneration rate (80%) and number of plantlet per calli. The established methods might be used for production of phytochemicals and plantlets in large scale.
In Vitro Micropropagation of a Valuable Medicinal Plant, Plectranthus amboinicus  [PDF]
Zuraida Ab Rahman, Erny Sabrina Mohd Noor, Mohd Shukri Mat Ali, Razali Mirad, Ayu Nazreena Othman
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.68113
Abstract: The effect of the plant growth regulators benzyl amino purine (BAP), 1-naphthaleneacetic acid (NAA) and kinetin (KIN) on in vitro shoot induction and proliferation of Plectranthus amboinicus was examined. Explants obtained from lateral shoots and apical shoots of P. amboinicus were inoculated on Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP, NAA and KIN. When the effect of each growth regulator was considered singly, the highest rate of shoot induction (80% of explants producing shoots) and highest number of shoots produced (2.4 shoots per explant) were obtained from lateral shoot explants cultured on MS media supplemented with 3.0 mg/L BAP within 6 - 7 weeks. Better results were obtained using MS medium supplemented with 1 mg/L BAP + 5 mg/L NAA. Shoot proliferation rose to 85%, while 5.7 shoots per explants were recorded. Among the different media tested for rooting, MS medium supplemented with 1.0 mg/L IBA was the most effective for root induction. The quality of the roots obtained was better than that obtained using MS media supplemented with NAA or IAA.
Optimizing Extraction of Phenolics and Flavonoids from Solanum ferox Fruit  [PDF]
Zuraida Ab Rahman, Mohd Waznul Adly Mohd Zaidan, Ayu Nazreena Othman, Muhamad Aizuddin Ahmad, Sanimah Simoh, Muhammad Aiman Haiqal Ismail
Natural Science (NS) , 2019, DOI: 10.4236/ns.2019.114011
Abstract: Various phenolic and flavonoid compounds that are secondary plant metabolites are known to contribute to physiological wellbeing. Extraction efficiency of such compounds from plant sources is dependent on the extraction solvent type and composition, and its pH. In this study, different extraction variables were examined: heating time (20 to 180 min), temperature (60°C, 75°C and 90°C) and pH (2.5, 3.0, 4.0, 5.0, 6.0 and 7.0). Hot water was used in the extraction of dry samples. For phenolics, the most efficient extraction was by using water at 60°C for 180 min, whereby 5.95 mg GA equivalent/dry extract was achieved. The most efficient extraction of flavonoids was achieved with water at 60°C for 150 min, whereby 43 μg Quercetin equivalent/dry extract was obtained. Adjusting the solvent to pH 2.5 increased the yield to 45.3 μg Quercetin equivalent/dry extract.
A Simple and Efficient Protocol for the Mass Propagation of Vanilla planifolia  [PDF]
Ab Rahman Zuraida, Kamarulzaman Hassan Fatin Liyana Izzati, Othman Ayu Nazreena, Wan Sembok Wan Zaliha, Che Mohd Zain Che Radziah, Zainal Zamri, Subramaniam Sreeramanan
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.49205
Abstract:

The present study describes a direct shoot regeneration-based micropropagation procedure for Vanilla planifolia. Two types of explant (i.e. shoot apex and stem nodal segment) were screened for their shoot induction potential following a three-month treatment with 6-benzylaminopurine (BAP) and α-Naphthaleneacetic acid (NAA). Results indicated that the shoot apices were poor candidates for shoot induction whereas the stem nodal segments showed potential for shoot initiation at a rate of up to 6 shoots/explant. Stem nodal segments were the most responsive as shoots formed (55 shoots) directly following treatments with 1 mg/L BAP at half strength MS medium after the third subculture. In addition, more shoots were produced on solid medium treatments compared to the liquid medium treatments in two strengths of mediums tested. Regenerated plantlets derived from the 1 mg/L of BAP treatment were induced to root following a one month culture in growth regulator-free MS medium. There was 90% survival rate of the rooted plantlets after acclimatization in the greenhouse. The findings in the present study would be helpful for large-scale mass propagation of Vanilla planifolia using this simple and efficient protocol.

In Vitro Regeneration of Curcuma caesia Plantlets from Basal Part and via Somatic Embryogenesis  [PDF]
Ab. Rahman Zuraida, Kamaruddin Fatin Liyana Izzati, Othman Ayu Nazreena, Che Mohd Zain Che Radziah, Sheikh Ghadzi Siti Nur Asyikin, Subramaniam Sreeramanan
Advances in Bioscience and Biotechnology (ABB) , 2014, DOI: 10.4236/abb.2014.54044
Abstract:

Plantlets of Curcuma caesia were produced in vitro from newly sprouting vegetative buds of tubers. Segments of the plantlets from the junction between the root and the basal portion of the stem were subsequently used as explants to investigate factors affecting callus induction and plant regeneration via somatic embryogenesis. The explants were placed on Woody Plant Medium (WPM) together with different concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and benzyl aminopurine (BAP) in the presence of light. The growth medium supplemented with 5 mg/L BAP and 2 mg/L 2,4-D promoted callus induction after 70 days of culture. Sub-culturing on the same medium enhanced the production of friable callus. Culture media containing higher concentrations of agar promoted the development of green somatic embryos from the callus. Respond of somatic embryogenesis was most successful with MS medium in 6.0 g/L agar supplemented with 5 mg/L BAP and 0.2 mg/L 2,4-D whereby the callus developed into green somatic embryos with an efficiency of 53%. This culture medium also produced the largest number plantlets.

Optimization of Extraction Conditions for Total Phenolics and Total Flavonoids from Kaempferia parviflora Rhizomes  [PDF]
Zuraida Ab Rahman, Shazwan Abd Shukor, Hartinee Abbas, Chandradevan A. L. Machap, Mohd Suhaimi Bin Alias, Razali Mirad, Syairah Sofiyanand, Ayu Nazreena Othman
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.95014
Abstract: Kaempferia parviflora plants derived from in vitro culture were grown in the glasshouse. A comparison of the yield of total phenolics and total flavonoids under varying extraction conditions from rhizomes harvested from plants of different ages was undertaken. The results showed that phenolic and flavonoid contents in the rhizomes were highest 8 months after planting. Another study found that 2 g rhizomes extracted in 50 ml of water at 90°C for 120 minutes gave the best yield of phenolics and flavonoids. Under these conditions, an average of 210 mg GAE/g dry weight of total phenolics and 81 μg QCE/g dry weight of total flavonoids were obtained.
Influence of Agitation Rate on the Growth of MD2 Pineapple Protocorm-Like Bodies and Shoots in Liquid-Shake Culture  [PDF]
Zuraida Ab Rahman, Hartinee Abbas, Ayu Nazreena Othman, Wan Zaliha Wan Sembok
American Journal of Plant Sciences (AJPS) , 2019, DOI: 10.4236/ajps.2019.107088
Abstract: The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L 1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80, 100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the highest fresh weight and the highest number of shoots at 76 g and 41 shoots, respectively. A comparative study of agitation found that 80 rpm was the best speed which enhanced both PLB and shoot formation. The findings in the present study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.
FLT3 Gene Mutation in Childhood Acute Leukemia: A Preliminary Study
Zefarina Zulkafli,Zuraida Zainun,Mohd Nazri Hassan,Wan Suriana Wan Ab Rahman,Nurul Azhan Othman,Selamah Ghazali,Rosline Hassan
New Journal of Science , 2014, DOI: 10.1155/2014/735453
Abstract: Introduction. FLT3 is a tyrosine kinase receptor involved in the proliferation and differentiation of hematopoietic stem cells. There are two types of common FLT3 gene mutation, internal tandem duplication and the D835 mutation, which are known to be associated with a poor clinical outcome in acute leukemia patients. Methods. This study evaluates the incidence of FMS-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) in 38 pediatric patients diagnosed with acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) in Hospital Universiti Sains Malaysia. DNA extraction was done from archive bone marrow samples to determine FLT3-ITD mutations using polymerase chain reaction. Results. In this pediatric series, the age ranges were 2–14 years. However, no FLT3-ITD mutations were detected in any of the samples. Conclusion. This preliminary study suggested that the incidence of FLT3 gene mutation most probably was very low in pediatrics patients diagnosed with acute leukemia. A further study with larger number of patient samples is necessary to confirm the findings and to further appreciate the prognostic value of FLT3-ITD mutation among pediatrics patients. 1. Introduction Fms-like tyrosine kinase 3 (FLT3) is a receptor tyrosine kinase expressed by immature hematopoietic cells. FLT3 ligand is expressed by marrow stromal cells and other cells and synergizes with other growth factors to stimulate proliferation and differentiation of stem cells, progenitor cells, dendritic cells, and natural killer cells. In normal hematopoietic cells, FLT3-ITDs (internal tandem duplications) mutations have not been detected in the cord blood and bone marrow [1–5]. In general, there are 2 types of FLT3 mutations which are internal tandem duplications (FLT3/ITD mutations) in or near the juxtamembrane domain of the receptor and point mutations resulting in single amino acid substitutions occurring within the activation loop of the tyrosine kinase domain (FLT3/TKD mutations). Both mutations are known to be associated with a poor clinical outcome in acute leukemia patients. The incidence of FLT3/ITD mutations varies according to age and clinical risk group, being less common in pediatric acute myeloid leukemia (AML) and in AML arising from an antecedent myelodysplastic syndrome. FLT3 mutations are genetic changes that have been reported to have prognostic significance in acute myeloid leukemia (AML) [6]. Study by Thiede et al. in 2002 analyzed the prevalence of FLT3-ITD mutations in 979 AML patients, with 20.4% found to be positive. However, the tandem duplication
Using Speech Recognition in Learning Primary School Mathematics via Explain, Instruct and Facilitate Techniques  [PDF]
Ab Rahman Ahmad, Sami M. Halawani, Samir K. Boucetta
Journal of Software Engineering and Applications (JSEA) , 2014, DOI: 10.4236/jsea.2014.74025
Abstract:

The application of Information and Communication Technologies has transformed traditional Teaching and Learning in the past decade to computerized-based era. This evolution has resulted from the emergence of the digital system and has greatly impacted on the global education and socio-cultural development. Multimedia has been absorbed into the education sector for producing a new learning concept and a combination of educational and entertainment approach. This research is concerned with the application of Window Speech Recognition and Microsoft Visual Basic 2008 Integrated/Interactive Development Environment in Multimedia-Assisted Courseware prototype development for Primary School Mathematics contents, namely, single digits and the addition. The Teaching and Learning techniques—Explain, Instruct and Facilitate are proposed and these could be viewed as instructors’ centered strategy, instructors’—learners’ dual communication and learners' active participation. The prototype is called M-EIF and deployed only users' voices; hence the activation of Window Speech Recognition is required prior to a test run.

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