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Search Results: 1 - 10 of 192914 matches for " Zoraida Díaz-Bello "
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Antígeno excreción-secreción de Fasciola hepatica: ultrafiltración y aplicación en inmunodiagnóstico
Colmenares,Cecilia; Méndez,Liber; Díaz-Bello,Zoraida; Alarcón de Noya,Belkisyolé;
Acta bioqu?-mica cl?-nica latinoamericana , 2007,
Abstract: low sensitivity in the coprologic diagnosis of human fasciolosis has motivated the development of immunodiagnosis. the excretion-secretion antigen from fasciola hepatica adult worms (esafh) with elisa is suitable for screening, although it overestimates its prevalence. however, when tested by western blot (wb) it does not show any optimal recognition of its components. in order to obtain a better preparation, the antigen was ultrafiltered by ym 10, 30 and 50 kda membranes. retentates (r) were used by elisa and wb. a higher discrimination between positives and negatives by elisa and a better resolution in the antigen recognition in wb was achieved with the r50 fraction. molecules of 9, 14, 65 kda, and region about 27 kda were detected with high sensitivity (90 to 100% of positive sera) and specificity (none of the negative sera). among the 29 sera with other parasitic diseases, only one with paragonimus sp. reacted to the 65 kda molecule. elisa-esafh with all filtrated fractions was useful, facilitating the infection screening even though the best results were obtained with r50. the verification of positive cases is efficiently achieved using the r50 of the esafh fraction by wb.
Antígeno excreción-secreción de Fasciola hepatica: ultrafiltración y aplicación en inmunodiagnóstico Fasciola hepatica excretion-secretion antigen: ultrafiltration and application in immunodiagnosis
Cecilia Colmenares,Liber Méndez,Zoraida Díaz-Bello,Belkisyolé Alarcón de Noya
Acta bioqu?-mica cl?-nica latinoamericana , 2007,
Abstract: La baja sensibilidad de la coprología en el diagnóstico de la fasciolosis humana ha motivado el desarrollo del inmunodiagnóstico. El antígeno de excreción-secreción de adultos de Fasciola hepatica (AFhES) en ELISA es adecuado para el screening, aunque sobrestima la prevalencia; cuando se utiliza en Western blot (WB) no muestra un buen reconocimiento de sus componentes. Para lograr una mejor preparación, se ultrafiltró el antígeno a través de membranas YM de 10, 30 y 50 kDa. Los retenidos (R) se usaron en ELISA y WB. La mayor discriminación entre positivos y negativos en ELISA y la mejor resolución en el reconocimiento al antígeno en WB, se logró con la fracción R50. Se destacan las moléculas de 9, 14, 65 kDa y la región alrededor de 27 kDa, detectadas con alta sensibilidad (90 al 100% de los sueros positivos) y especificidad (por ningún negativo). Al ensayar 29 sueros con otras parasitosis, sólo el de una persona con Paragonimus sp. reaccionó a la molécula de 65 kDa. ELISA-AFhES con todas las fracciones filtradas fue útil, facilitando el screening de la infección, aunque con R50 se obtuvieron los mejores resultados. La comprobación de los casos positivos se logra eficientemente utilizando la fracción R50 del AFhES en WB. Low sensitivity in the coprologic diagnosis of human fasciolosis has motivated the development of immunodiagnosis. The excretion-secretion antigen from Fasciola hepatica adult worms (ESAFh) with ELISA is suitable for screening, although it overestimates its prevalence. However, when tested by Western blot (WB) it does not show any optimal recognition of its components. In order to obtain a better preparation, the antigen was ultrafiltered by YM 10, 30 and 50 kDa membranes. Retentates (R) were used by ELISA and WB. A higher discrimination between positives and negatives by ELISA and a better resolution in the antigen recognition in WB was achieved with the R50 fraction. Molecules of 9, 14, 65 kDa, and region about 27 kDa were detected with high sensitivity (90 to 100% of positive sera) and specificity (none of the negative sera). Among the 29 sera with other parasitic diseases, only one with Paragonimus sp. reacted to the 65 kDa molecule. ELISA-ESAFh with all filtrated fractions was useful, facilitating the infection screening even though the best results were obtained with R50. The verification of positive cases is efficiently achieved using the R50 of the ESAFh fraction by WB.
Inmunodiagnóstico en Didelphis marsupialis usando un antígeno de Paragonimus de Venezuela Immunodiagnostic in Didelphis marsupialis using an antigen of Venezuelan Paragonimus
Erika Gómez Martínez,Zoraida Díaz-Bello,Reinaldo Zavala-Jaspe,Marcos Tulio Díaz
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: Los Paragonimus son trematodos que habitualmente viven en los pulmones de mamíferos carnívoros y omnívoros, entre ellos el hombre. En el oriente venezolano se encuentra el único foco de Paragonimus sp. donde Didelphis marsupialis es el único reservorio demostrado hasta ahora. Con el fin de tener herramientas de inmunodiagnóstico que detecten la presencia de Paragonimus sp. en esta especie, se elaboraron varios reactivos para realizar un ensayo inmunoenzimático ELISA. Entre ellos se obtuvo un antígeno crudo soluble de vermes adultos de Paragonimus y una inmunoglobulina de gallina anti-IgG de Didelphis marsupialis. Los mismos se capturaron en la localidad de Aguas Blancas, municipio Montes, estado Sucre, Venezuela, y se obtuvieron muestras sanguíneas; en el caso de estar infectados, los vermes adultos se extrajeron del pulmón. Los parásitos se homogenizaron y ultracentrifugaron para obtener la fracción soluble del parásito (FSPA) como antígeno para el ELISA y Western blot y detectar los anticuerpos en los Didelphis marsupialis. El análisis electroforético mostró 22 moléculas entre 6 y 82 kDa; por Western blot se presentó un reconocimiento antigénico de 8 moléculas siendo las de 112 kDa y 268 kDa las más reconocidas por los sueros positivos. Los sueros negativos no reconocieron ninguna proteína. La producción de IgY en gallinas permitió desarrollar las técnicas de inmunodiagnóstico para la búsqueda de anticuerpos específicos anti-Paragonimus sp. en Didelphis, cuya aplicación permitirá establecer la vigilancia epidemiológica de estos reservorios en áreas endémicas sin sacrificio de los mismos. Paragonimus are trematodes that normally live in the lungs of carnivorous and omnivorous mammals such as humans. An outbreak of Paragonimus sp. in which Didelphis marsupialis was the only wild reservoir incriminated was described in eastern Venezuela. In order to have immunological tools to detect the presence of Paragonimus sp. in this reservoir, a whole antigen of the adult worm of this parasite was elaborated. Didelphis marsupialis were captured in the locality of Aguas Blancas, Montes municipality, Sucre state, Venezuela, from which blood samples were obtained and a search for worms was performed in lungs. Worms were homogenized and ultracentrifugated to obtain FSPA to perform immunoassay (ELISA) to detect antibodies in opossums. The electrophoresis analysis showed a pattern of 22 molecules between 6 and 82 kDa; by western blot, the antigenic recognition of 8 antigenic molecules appeared,112 kDa and 268 kDa molecules being the most strongly recognized by positive
Diagnóstico confirmatorio de anticuerpos anti-Trypanosoma cruzi en donantes referidos por bancos de sangre en Venezuela A confirmatory diagnosis of antibodies anti-Trypanosoma cruzi in donors referred by blood banks in Venezuela
Zoraida Díaz-Bello,Reinaldo Zavala-Jaspe,María Díaz-Villalobos,Luciano Mauriello
Investigación Clínica , 2008,
Abstract: Con el objetivo de establecer el diagnóstico confirmatorio para Trypanosoma cruzi se realizaron al menos dos pruebas inmunoserológicas (ELISA, Reacción de Hemoaglutinación Indirecta, RHI, o Reacción de Fijación de Complemento, RFC) a donantes provenientes de bancos de sangre de centros asistenciales públicos y privados de Venezuela que acudieron durante 48 meses entre los a os 1997-1998 y 2003-2004 a la Sección de Inmunología del Instituto de Medicina Tropical en Caracas, Venezuela. Se evaluaron 254 donantes referidos de diferentes bancos de sangre por presentar anticuerpos anti-T. cruzi en pruebas de despistaje. Se confirmó la presencia de anticuerpos en 129/254 (50,79%) de los individuos por las técnicas de ELISA-IgG o RHI y RFC. El “xenodiagnóstico artificial” fue positivo en 10/118 (8,5%) personas con serología positiva. De 129 donantes encontrados reactivos por técnicas serológicas, 68 eran residentes de la región capital y 61 del interior del país. Así mismo, 113 nacieron en el interior del país, 8 en Caracas y 8 en Colombia. En 12 individuos confirmados serológicamente se constató la donación de sangre en mínimo 4 ocasiones antes de detectar la infección. El presente estudio resalta la importancia de la búsqueda activa de individuos con Enfermedad de Chagas a través de la detección de anticuerpos contra T. cruzi en la evaluación integral de donantes de sangre para descartar el riesgo de transmisión a otras personas. Muchos de estos donantes con anticuerpos anti-T. cruzi, la gran mayoría clínicamente asintomáticos, habían donado sangre en varias ocasiones previas al diagnóstico. To establish the confirmatory diagnosis of Trypanosoma cruzi infection, at least two immunoserological tests (ELISA, Indirect hamaglutination, IH, Complement Fixation Test, CFT) were carried out in 254 donors, from public and private blood banks of Venezuela, during 48 months between 1997-1998 and 2003-2004, referred to the Immunology Section of the Tropical Medicine Institute in Caracas. Antibodies anti-T. cruzi were detected in 129/254 (50,79%) by ELISA-IgG or IH and CFT. The “artificial xenodiagnosis” was positive in 10/118 persons with positive confirmed serology. Of 129 donors found positive by the serological tests, 68 were living in the capital region and 61 in the interior of the country. Likewise 113 were born in the interior of the country, 8 in Caracas and 8 in Colombia. Of them, 12 individuals serologically confirmed declared to have donated blood in a minimum of 4 occasions before diagnosis. The present study emphasizes the importance of detection of antibodie
A mucin like gene different from the previously reported members of the mucin like gene families is transcribed in Trypanosoma cruzi but not in Trypanosoma rangeli
Abate, Teresa;Rincón, Mónica;Díaz-Bello, Zoraida;Spencer, Lilian;Rodríguez-Acosta, Alexis;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000400009
Abstract: trypanosoma cruzi expresses mucin like glycoproteins encoded by a complex multigene family. in this work, we report the transcription in t. cruzi but not in t. rangeli of a mucin type gene automatically annotated by the t. cruzi genome project. the gene showed no nucleotide similarities with the previously reported t. cruzi mucin like genes, although the computational analysis of the deduced protein showed that it has the characteristic features of mucins: a signal peptide sequence, o-glycosylation sites, and glycosylphosphatidylinositol (gpi) anchor sequence. the presence in this gene of n- terminal and c- terminal coding sequences common to other annotated mucin like genes suggests the existence of a new mucin like gene family.
Enfermedad de Chagas de transmisión oral: vinculación del caso índice con una microepidemia urbana en Venezuela
Alarcón de Noya,Belkisyolé; Díaz-Bello,Zoraida; Colmenares,Cecilia; Ruiz-Guevara,; Noya,Oscar;
Boletín de Malariología y Salud Ambiental , 2010,
Abstract: the clinical, laboratory and epidemiological investigations that established the connection between the index case of an acute episode of chagas disease and an orally acquired outbreak in an urban school in caracas are described. the strategy used for early diagnosis and treatment of this medical emergency prevented major clinical severity and mortality.
Paragonimiasis pulmonar: Descripción de un caso Pulmonary paragonimiasis: Case report
Salha Abdul-Hadi,Zoraida Díaz-Bello,Reinaldo Zavala-Jaspe,Marieli Rangel-Lujano
Investigación Clínica , 2008,
Abstract: La Paragonimiasis es una zoonosis parasitaria de diversos animales silvestres o domésticos y del hombre, causada por especies de tremátodes del género Paragonimus siendo el Paragonimus westermani el que más infecta al hombre. El humano se infecta al ingerir crustáceos de agua dulce parasitados (cangrejos de río) crudos o insuficientemente cocidos. El objetivo de este trabajo es describir el caso de un preescolar masculino de 3 a os y medio de edad, natural y procedente del estado Guárico quien vivió un a o en zona costera del Ecuador (Provincia Manabí) donde consumió alimentos de la zona como ceviche. Consultó por dificultad respiratoria y durante la hospitalización se evidenció hepatomegalia y presencia de nódulos subcutáneos en espalda. La Tomografía Axial computarizada (TAC) de tórax reveló importante infiltrado a nivel de ambas bases pulmonares a predominio del lado derecho con derrame y engrosamiento pleural. Por la clínica, las imágenes radiológicas, la eosinofilia (47% con contaje absoluto de eosinófilos (CAE) 6.862/mm3) y el antecedente de ingesta de cangrejos crudos 6 meses antes, se sugirió descartar paragonimiasis pulmonar. En un estudio seriado de muestras de sueros se evidenció la presencia de anticuerpos específicos anti-Paragonimus por ELISA y Western blot, sin embargo no se encontraron huevos del parásito en heces o en esputo. Se indicó tratamiento con praziquantel 25 mg/kg de peso 3 tomas al día durante 3 días con lo cual desapareció la sintomatología, mejoraron las imágenes radiológicas y disminuyó el contaje de eosinófilos. Paragonimiasis is a zoonosis affecting wild and domestic animals and human beings, caused by species of trematodes of the genus Paragonimus. Humans become infected after ingestion of raw or poorly cooked fresh water crustaceans. The aim of the present work is the description of a case of a 3-years old child, coming from Guárico State in Venezuela with a year of residence in the seashore of the Provincia Manabí in Ecuador, where he ate crabs in “ceviche”. During hospitalization, he presented respiratory distress, hepatomegaly and nodules in the back. The thorax cat scan showed heavy infiltrate in both pulmonary bases and pleural compromise. Based on clinic, radiological images, an eosinophilia of 47% (Eosinophils absolute count (EAC) 6.682/mm3) and the antecedent of raw crabs ingestion, pulmonary paragonimiasis was diagnosed. Paragonimus eggs were not found in sputum and feces. ELISA with crude Paragonimus antigen was positive and Western blot revealed recognition of specific molecules. After treatment for three day
Diagnóstico confirmatorio de anticuerpos anti-Trypanosoma cruzi en donantes referidos por bancos de sangre en Venezuela
Díaz-Bello,Zoraida; Zavala-Jaspe,Reinaldo; Díaz-Villalobos,María; Mauriello,Luciano; Maekelt,Alberto; Alarcón de Noya,Belkisyolé;
Investigación Clínica , 2008,
Abstract: to establish the confirmatory diagnosis of trypanosoma cruzi infection, at least two immunoserological tests (elisa, indirect hamaglutination, ih, complement fixation test, cft) were carried out in 254 donors, from public and private blood banks of venezuela, during 48 months between 1997-1998 and 2003-2004, referred to the immunology section of the tropical medicine institute in caracas. antibodies anti-t. cruzi were detected in 129/254 (50,79%) by elisa-igg or ih and cft. the ?artificial xenodiagnosis? was positive in 10/118 persons with positive confirmed serology. of 129 donors found positive by the serological tests, 68 were living in the capital region and 61 in the interior of the country. likewise 113 were born in the interior of the country, 8 in caracas and 8 in colombia. of them, 12 individuals serologically confirmed declared to have donated blood in a minimum of 4 occasions before diagnosis. the present study emphasizes the importance of detection of antibodies against t. cruzi in the integral evaluation of blood donors, since many of them with antibodies anti-t. cruzi, have donated blood several times previous to diagnosis.
Inmunodiagnóstico en Didelphis marsupialis usando un antígeno de Paragonimus de Venezuela
Gómez Martínez,Erika; Díaz-Bello,Zoraida; Zavala-Jaspe,Reinaldo; Tulio Díaz,Marcos; Noya,Oscar; Alarcón de Noya,Belkisyole;
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: paragonimus are trematodes that normally live in the lungs of carnivorous and omnivorous mammals such as humans. an outbreak of paragonimus sp. in which didelphis marsupialis was the only wild reservoir incriminated was described in eastern venezuela. in order to have immunological tools to detect the presence of paragonimus sp. in this reservoir, a whole antigen of the adult worm of this parasite was elaborated. didelphis marsupialis were captured in the locality of aguas blancas, montes municipality, sucre state, venezuela, from which blood samples were obtained and a search for worms was performed in lungs. worms were homogenized and ultracentrifugated to obtain fspa to perform immunoassay (elisa) to detect antibodies in opossums. the electrophoresis analysis showed a pattern of 22 molecules between 6 and 82 kda; by western blot, the antigenic recognition of 8 antigenic molecules appeared,112 kda and 268 kda molecules being the most strongly recognized by positive sera. the negative sera did not recognize any band. the production of igy in chicken enabled the development of reagents capable of performing a standard immunodiagnosis technique to find specific anti-paragonimus sp. in didelphis marsupialis in order to establish epidemiological surveillance of these reservoirs in endemic areas.
Paragonimiasis pulmonar: Descripción de un caso
Abdul-Hadi,Salha; Díaz-Bello,Zoraida; Zavala-Jaspe,Reinaldo; Rangel-Lujano,Marieli; Gómez,Erika; Figueira,Ivonne; Alarcón-Noya,Belkisyolé;
Investigación Clínica , 2008,
Abstract: paragonimiasis is a zoonosis affecting wild and domestic animals and human beings, caused by species of trematodes of the genus paragonimus. humans become infected after ingestion of raw or poorly cooked fresh water crustaceans. the aim of the present work is the description of a case of a 3-years old child, coming from guárico state in venezuela with a year of residence in the seashore of the provincia manabí in ecuador, where he ate crabs in ?ceviche?. during hospitalization, he presented respiratory distress, hepatomegaly and nodules in the back. the thorax cat scan showed heavy infiltrate in both pulmonary bases and pleural compromise. based on clinic, radiological images, an eosinophilia of 47% (eosinophils absolute count (eac) 6.682/mm3) and the antecedent of raw crabs ingestion, pulmonary paragonimiasis was diagnosed. paragonimus eggs were not found in sputum and feces. elisa with crude paragonimus antigen was positive and western blot revealed recognition of specific molecules. after treatment for three days with praziquantel at a daily dose of 25 mg/kg body weight divided into three intakes, the sintomatology disappeared and radiological images and number of eosinophils diminished considerably.
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