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Search Results: 1 - 10 of 177136 matches for " Zoilo Pires de Camargo "
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Trichosporon species isolated from the perigenital region, urine and catheters of a Brazilian population
Silvestre Junior, Agenor Messias;Miranda, Mauricio Alexandre Bandeira Rampazzo;Camargo, Zoilo Pires de;
Brazilian Journal of Microbiology , 2010, DOI: 10.1590/S1517-83822010000300013
Abstract: the present study aimed to 1) determine the colonization rates of medically important trichosporon species on normal perigenital skin and 2) determine the isolation rates of trichosporon spp. isolated from the urine and catheters of brazilian patients hospitalized in the intensive care unit (icu). the overall colonization rate of trichosporon spp. was 11.15% (112 isolates). the most common species isolated from normal perigenital skin was t. cutaneum (29.46%), followed by t. asteroides (20.53%), t. ovoides (15.17%), t. inkin (10.71%), t. mucoides (8.92%), and t. asahii (6.25%). from urine and catheters, t. asahii was the species most commonly isolated (76.5%; n =23), followed by t. inkin (16.6%; n = 5) and t. asteroides (6.6%; n = 2). in addition, the highest isolation rate occurred in subjects in the 71- to 80-year-old age range (36.7%; n= 11), followed by 61 to 70 (26.7%; n = 8), 51 to 60 (13.3%; n = 4), 31 to 40 (13.33%; n = 4), and 41 to 50 (10%; n =3). we concluded that 6 medically important species of the genus trichosporon colonize the perigenital region in a normal population. the identification of these species is possible by means of classical methods but often requires repeated analyses repetitions due to difficulties in the assimilation process. in contrast, only 3 species of trichosporon were isolated from urine and catheters.
Detection of antibodies to Paracoccidioides brasiliensis in horses from northern Region of Paraná State / Detec o de anticorpos para Paracoccidioides brasiliensis em cavalos da regi o norte do Estado do Paraná
Andréia Corrêa Corte,Eiko Nakagawa Itano,Roberta Lemos Freire,Zoilo Pires de Camargo
Semina : Ciências Agrárias , 2009,
Abstract: Paracoccidioidomycosis, caused by the dimorphic fungus Paracoccidioides brasiliensis, is the most prevalent human systemic mycosis in Latin America. Paracoccidioidomycosis affects mainly male rural workers, causing granulomatous lesions in organs such as lungs, liver and spleen. The participation of other animal species in the fungus eco-epidemiology in not well understood. The aim of this study was to evaluate the infection by P. brasiliensis in horses from the Northern Region of Paraná State. The serum samples from 100 horses were assayed by ELISA and Immunodiffusion test, using gp43 and exoantigen as antigens, respectively. A seropositivity of 30% was observed by ELISA test, although all samples were negative by immunodiffusion test. These results suggest that horses can be indicator of fungus presence in the ambient. A paracoccidioidomicose, causada pelo fungo dimórfico Paracoccidioides brasiliensis, é a micose sistêmica de maior prevalência em humanos na América Latina. A paracoccidioidomicose afeta principalmente trabalhadores rurais do sexo masculino, provocando les es granulomatosas em órg os como pulm es, fígado e ba o. A participa o de outras espécies de animais na eco-epidemiologia do fungo n o é bem compreendida. Este trabalho teve como objetivo avaliar a infec o por P. brasiliensis em cavalos da Regi o Norte do Estado do Paraná. Foram analisadas 100 amostras de soros de cavalos por ELISA e por Imunodifus o em gel de agar, utilizando como antígenos a gp43 e o exoantígeno de P. brasiliensis, respectivamente. A soropositividade observada foi de 30% por ELISA enquanto que por imunodifus o todos os soros foram negativos. Os resultados obtidos sugerem que cavalos podem ser indicadores da presen a do fungo no meio ambiente.
Natural antibodies in paracoccidioidomycosis
Unterkircher, Carmelinda S.;Le?o, Mariella V.P.;Blotta, Maria Heloisa S.L.;Camargo, Zoilo Pires de;
Brazilian Journal of Microbiology , 2004, DOI: 10.1590/S1517-83822004000100009
Abstract: recent attention has been focused on the natural antibodies as a component of natural immunity and as integral part of the idiotypic network. however, their functional role in different infections has rarely been studied. this work was undertaken to investigate the presence of natural antibodies in paracoccidioidomycosis (pcm). in addition, we analyzed anti-p. brasiliensis antibodies and their distribution in igg subclasses in order to acquire better knowledge about the humoral immune response in this mycosis. our findings show that the natural antibody response is not very much increased in pcm when compared with other parasite infections and this response is restricted to a few specificities, suggesting that p. brasiliensis moderately triggers cd5+ b cells. the anti-actin antibody was the main antibody specificity found in pcm. specific antibodies to p. brasiliensis were mainly found in the igg1 subclass in chronic patients of pcm.
Paracoccidioidomicose em índios brasileiros da tribo Suruí: estudo clínico-laboratorial de 2 casos
Forjaz, Marly H.H.;Fischman, Olga;Camargo, Zoilo Pires de;Vieira Filho, Jo?o Paulo Botelho;Colombo, Arnaldo Lopes;
Revista da Sociedade Brasileira de Medicina Tropical , 1999, DOI: 10.1590/S0037-86821999000500015
Abstract: paracoccidioidomycosis has been considered the most frequent endemic systemic mycosis in latin america. although most cases of paracoccidioidomycosis involve rural workers, this systemic fungal disease has been scarcely reported among amerindian populations from brazil. we report two cases of paracoccidioidomycosis in tupi-mondé amerindians from cacoal, state of rond?nia, brazil. both cases exhibited positive serological results by a specific immunodiffusion test only when the assay was performed with antigens obtained from the mycelial form of p. brasiliensis. the authors present a literature review of paracoccidioidomycosis in brazilian amerindians and discuss the need for further investigations about the impact of the antigenic diversity of p. brasiliensis from different geographic areas on the serological diagnosis of pcm.
Paracoccidioidomicose em índios brasileiros da tribo Suruí: estudo clínico-laboratorial de 2 casos
Forjaz Marly H.H.,Fischman Olga,Camargo Zoilo Pires de,Vieira Filho Jo?o Paulo Botelho
Revista da Sociedade Brasileira de Medicina Tropical , 1999,
Abstract: Paracoccidioidomicose é considerada a micose sistêmica endêmica mais prevalente na América Latina. Apesar da maior parte da casuística de paracoccidioidomicose ocorrer entre trabalhadores rurais, há poucos casos documentados de ocorrência dessa micose entre índios brasileiros. S o apresentados 2 casos de paracoccidioidomicose em índios Suruí, família linguística Tupi-Mondé, procedentes de Cacoal, Rond nia. Ambos apresentaram sorologia positiva à imunodifus o apenas com antígenos da fase miceliana do P. brasiliensis. Os autores apresentam revis o de literatura sobre a ocorrência dessa micose entre índios brasileiros e discutem a necessidade de futuras investiga es buscando caracterizar as diferen as regionais de cepas de P. brasiliensis e seu impacto no diagnóstico sorológico dessa micose.
Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
Batista Júnior, Jo?o;Berzaghi, Rodrigo;Arnaud, Adelita Duanra Medeiros do Monte;Fontes, Cor Jesus Fernandes;Camargo, Zoilo Pires de;Hahn, Rosane Christine;
Memórias do Instituto Oswaldo Cruz , 2010, DOI: 10.1590/S0074-02762010000100009
Abstract: this study is the first report on genetic differences between isolates of paracoccidioides brasiliensis from a single patient. we describe a simultaneous infection with genetically distinct isolates of p. brasiliensis in a patient with chronic paracoccidioidomycosis. the clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic dna (rapd) analysis. the rapd technique can be helpful for distinguishing between clinical isolates. different random primers were used to characterise these clinical isolates. the rapd patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28% genetic variability in this fungal species, opening new possibilities for clinical studies of p. brasiliensis. based on these results and preliminary clinical findings, we suggest that different genotypes of p. brasiliensis might infect the same patient, inducing the active form of the disease.
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Gregory Gegembauer,Leticia Mendes Araujo,Edy Firmina Pereira,Anderson Messias Rodrigues,Anamaria Mello Miranda Paniago,Rosane Christine Hahn,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2014, DOI: 10.1371/journal.pntd.0002986
Abstract: Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. In western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.
Phylogenetic Analysis Reveals a High Prevalence of Sporothrix brasiliensis in Feline Sporotrichosis Outbreaks
Anderson Messias Rodrigues,Marcus de Melo Teixeira,G. Sybren de Hoog,Tania Maria Pacheco Schubach,Sandro Antonio Pereira,Geisa Ferreira Fernandes,Leila Maria Lopes Bezerra,Maria Sueli Felipe,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2013, DOI: 10.1371/journal.pntd.0002281
Abstract: Sporothrix schenckii, previously assumed to be the sole agent of human and animal sporotrichosis, is in fact a species complex. Recently recognized taxa include S. brasiliensis, S. globosa, S. mexicana, and S. luriei, in addition to S. schenckii sensu stricto. Over the last decades, large epidemics of sporotrichosis occurred in Brazil due to zoonotic transmission, and cats were pointed out as key susceptible hosts. In order to understand the eco-epidemiology of feline sporotrichosis and its role in human sporotrichosis a survey was conducted among symptomatic cats. Prevalence and phylogenetic relationships among feline Sporothrix species were investigated by reconstructing their phylogenetic origin using the calmodulin (CAL) and the translation elongation factor-1 alpha (EF1α) loci in strains originated from Rio de Janeiro (RJ, n = 15), Rio Grande do Sul (RS, n = 10), Paraná (PR, n = 4), S?o Paulo (SP, n = 3) and Minas Gerais (MG, n = 1). Our results showed that S. brasiliensis is highly prevalent among cats (96.9%) with sporotrichosis, while S. schenckii was identified only once. The genotype of Sporothrix from cats was found identical to S. brasiliensis from human sources confirming that the disease is transmitted by cats. Sporothrix brasiliensis presented low genetic diversity compared to its sister taxon S. schenckii. No evidence of recombination in S. brasiliensis was found by split decomposition or PHI-test analysis, suggesting that S. brasiliensis is a clonal species. Strains recovered in states SP, MG and PR share the genotype of the RJ outbreak, different from the RS clone. The occurrence of separate genotypes among strains indicated that the Brazilian S. brasiliensis epidemic has at least two distinct sources. We suggest that cats represent a major host and the main source of cat and human S. brasiliensis infections in Brazil.
Serological Diagnosis of Paracoccidioidomycosis: High Rate of Inter-laboratorial Variability among Medical Mycology Reference Centers
Monica Scarpelli Martinelli Vidal,Gilda Maria Barbaro Del Negro,Adriana Pardini Vicentini,Teresinha Inez Estivalet Svidzinski,Maria Jose Mendes-Giannini,Ana Marisa Fusco Almeida,Roberto Martinez,Zoilo Pires de Camargo,Carlos Pelleschi Taborda,Gil Benard
PLOS Neglected Tropical Diseases , 2014, DOI: 10.1371/journal.pntd.0003174
Abstract: Background Serological tests have long been established as rapid, simple and inexpensive tools for the diagnosis and follow-up of PCM. However, different protocols and antigen preparations are used and the few attempts to standardize the routine serological methods have not succeeded. Methodology/Principal findings We compared the performance of six Brazilian reference centers for serological diagnosis of PCM. Each center provided 30 sera of PCM patients, with positive high, intermediate and low titers, which were defined as the “reference” titers. Each center then applied its own antigen preparation and serological routine test, either semiquantitative double immunodifusion or counterimmmunoelectrophoresis, in the 150 sera from the other five centers blindly as regard to the “reference” titers. Titers were transformed into scores: 0 (negative), 1 (healing titers), 2 (active disease, low titers) and 3 (active disease, high titers) according to each center's criteria. Major discordances were considered between scores indicating active disease and scores indicating negative or healing titers; such discordance when associated with proper clinical and other laboratorial data, may correspond to different approaches to the patient's treatment. Surprisingly, all centers exhibited a high rate of “major” discordances with a mean of 31 (20%) discordant scores. Alternatively, when the scores given by one center to their own sera were compared with the scores given to their sera by the remaining five other centers, a high rate of major discordances was also found, with a mean number of 14.8 sera in 30 presenting a discordance with at least one other center. The data also suggest that centers that used CIE and pool of isolates for antigen preparation performed better. Conclusion There are inconsistencies among the laboratories that are strong enough to result in conflicting information regarding the patients' treatment. Renewed efforts should be promoted to improve standardization of the serological diagnosis of PCM.
Proteomics-Based Characterization of the Humoral Immune Response in Sporotrichosis: Toward Discovery of Potential Diagnostic and Vaccine Antigens
Anderson Messias Rodrigues?,Geisa Ferreira Fernandes?,Leticia Mendes Araujo?,Paula Portella Della Terra?,Priscila Oliveira dos Santos?,Sandro Antonio Pereira?,Tania Maria Pacheco Schubach?,Eva Burger?,Leila Maria Lopes-Bezerra?,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2015, DOI: 10.1371/journal.pntd.0004016
Abstract: Background Sporothrix schenckii and associated species are agents of human and animal sporotrichosis that cause large sapronoses and zoonoses worldwide. Epidemiological surveillance has highlighted an overwhelming occurrence of the highly pathogenic fungus Sporothrix brasiliensis during feline outbreaks, leading to massive transmissions to humans. Early diagnosis of feline sporotrichosis by demonstrating the presence of a surrogate marker of infection can have a key role for selecting appropriate disease control measures and minimizing zoonotic transmission to humans. Methodology We explored the presence and diversity of serum antibodies (IgG) specific against Sporothrix antigens in cats with sporotrichosis and evaluated the utility of these antibodies for serodiagnosis. Antigen profiling included protein extracts from the closest known relatives S. brasiliensis and S. schenckii. Enzyme-linked immunosorbent assays and immunoblotting enabled us to characterize the major antigens of feline sporotrichosis from sera from cats with sporotrichosis (n = 49), healthy cats (n = 19), and cats with other diseases (n = 20). Principal Findings Enzyme-linked immunosorbent assay-based quantitation of anti-Sporothrix IgG exhibited high sensitivity and specificity in cats with sporotrichosis (area under the curve, 1.0; 95% confidence interval, 0.94–1; P<0.0001) versus controls. The two sets of Sporothrix antigens were remarkably cross-reactive, supporting the hypothesis that antigenic epitopes may be conserved among closely related agents. One-dimensional immunoblotting indicated that 3-carboxymuconate cyclase (a 60-kDa protein in S. brasiliensis and a 70-kDa protein in S. schenckii) is the immunodominant antigen in feline sporotrichosis. Two-dimensional immunoblotting revealed six IgG-reactive isoforms of gp60 in the S. brasiliensis proteome, similar to the humoral response found in human sporotrichosis. Conclusions A convergent IgG-response in various hosts (mice, cats, and humans) has important implications for our understanding of the coevolution of Sporothrix and its warm-blooded hosts. We propose that 3-carboxymuconate cyclase has potential for the serological diagnosis of sporotrichosis and as target for the development of an effective multi-species vaccine against sporotrichosis in animals and humans.
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