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Search Results: 1 - 10 of 58730 matches for " ZhongNan Yang "
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Construction of a gene regulatory network for Arabidopsis based on metabolic pathway data
QingJu Jiao,ZhongNan Yang,JiFeng Huang
Chinese Science Bulletin , 2010, DOI: 10.1007/s11434-009-0728-8
Abstract: Elucidation of gene regulatory networks is the key to understanding the complex interplay of transcription factors (TFs) in the growth and propagation of organisms. In this work, we applied the theory that genes belonging to the same pathway are co-expressed, and therefore a promoter analysis of Arabidopsis genes could predict the transcriptional relationships between metabolic pathway genes. Using this approach, a total of 2268 TF-gene pairs were analyzed, 91 of which were characterized as highly confident, and 4 were confirmed by previously published experimental data. These results suggest that the predictions by this model are reliable. Furthermore, we demonstrated that the use of metabolic pathways to interpret gene regulatory networks of Arabidopsis has the potential to improve our understanding of the role of these processes in plant development and to identify biological functions of unknown genes.
Prediction of anther-expressed gene regulation in Arabidopsis
JiFeng Huang,JingJin Yang,Guan Wang,QingBo Yu,ZhongNan Yang
Chinese Science Bulletin , 2008, DOI: 10.1007/s11434-008-0381-7
Abstract: Anther development in Arabidopsis, a popular model plant for plant biology and genetics, is controlled by a complex gene network. Despite the extensive use of this genus for genetic research, little is known about its regulatory network. In this paper, the direct transcriptional regulatory relationships between genes expressed in Arabidopsis anther development were predicted with an integrated bioinformatic method that combines mining of microarray data with promoter analysis. A total of 7710 transcription factor-gene pairs were obtained. The 80 direct regulatory relationships demonstrating the highest confidence were screened from the initial 7710 pairs; three of the 80 were validated by previous experiments. The results indicate that our predicted results were reliable. The regulatory relationships revealed by this research and described in this paper may facilitate further investigation of the molecular mechanisms of anther development. The bioinformatic method used in this work can also be applied to the prediction of gene regulatory relationships in other organisms.
Research on the Contents and Indexes of Reservoir Operational Security  [PDF]
Jingchun Feng, Zhongnan Duan
Journal of Water Resource and Protection (JWARP) , 2010, DOI: 10.4236/jwarp.2010.211115
Abstract: Reservoirs play an important role in the development of economy and society, as well as the maintenance of ecological balance. The reservoir operational security can make every function of reservoirs fully played. This paper makes a systematic analysis on the meaning of reservoir operational security and builds up a framework system of it from the perspective of organization and system. On this3 basis, the paper researches the contents and indexes of reservoir operational security from the microscopic, intermediate and macroscopic aspects. The results of this paper provide a foundation for further research on reservoir operational security management.
OsMYB103 is required for rice anther development by regulating tapetum development and exine formation
Sen Zhang,ZiJun Fang,Jun Zhu,JuFang Gao,ZhongNan Yang
Chinese Science Bulletin , 2010, DOI: 10.1007/s11434-010-4087-2
Abstract: The Arabidopsis AtMYB103 gene is required for anther development, but whether the homologous gene in rice has the same role is unclear. Sequence analysis indicated that the rice OsMYB103 gene shares a high sequence similarity with AtMYB103. Therefore, we investigated the functional role of OsMYB103 in anther development using an RNAi approach. The OsMYB103 RNA transcript was expressed most abundantly in flowers, specifically in the tapetum, premeiotic pollen mother cells, and meiotic PMCs. OsMYB103-RNAi transgenic lines grew normally during their vegetative phase but displayed reduced male fertility, a phenotype that was associated with downregulated OsMYB103 transcript levels. Expression of OsMS2, an ortholog of the Arabidopsis AtMS2 gene, was also dramatically reduced in the transgenic plants. Knockdown of OsMYB103 led to defects in tapetum development, and most of the microspores in mature anthers lacked exines. Moreover, OsMYB103 could partially rescue the male sterility phenotype of an AtMYB103 knockout mutant ms188. Taken together, these results indicate that OsMYB103 does have an important role in rice tapetum and microspore development.
Quantitative trait loci for leaf chlorophyll content at two developmental stages of rice (Oryza sativa L.)
Yanjun Dong,Zhongnan Yang,Jianlong Xu,Dongzhi Lin
Communications in Biometry and Crop Science , 2007,
Abstract: Knowledge of the genetics of leaf chlorophyll content (LCC) at tillering and heading stages should help develop rice varieties with high photosynthetic ability. In this study, 182 backcross-recombinant inbred lines (BIL), derived from Koshihikari (japonica)/Kasalath (indica)//Koshihikari, were used to identify quantitative trait loci (QTL) for LCC at the tillering and heading stages of rice. Continuous variation and transgressive segregation for LCC were observed in the BIL population, indicating that LCC was a quantitatively inherited trait. Seven QTL for LCC were identified and mapped to chromosomes 1 (two QTL), 2, 3, 4, 6, and 8, which individually accounted for 5.1 to 14.8% of the total phenotypic variation. Three QTL (qLCC-1-1, qLCC-1-2 and qLCC-4) were common between the tillering and heading stages. The alleles at four QTL (qLCC-1-1, qLCC-1-2, qLCC-2, and qLCC-8) from Koshihikari and the alleles at the other three QTL (qLCC-3, qLCC-4 and qLCC-6) from Kasalath increased LCC. The tightly linked molecular markers flanking the QTL detected in this study should be useful in improving photosynthetic ability in rice.
Diffusion Entropy Approach to Dynamical Characteristics of a Hodgkin-Huxley Neuron
Huijie Yang,Fangcui Zhao,Zhongnan Li,Wei Zhang
Quantitative Biology , 2003, DOI: 10.1016/j.physa.2004.08.017
Abstract: By means of the concept DE we analyze the responses of a HH neuron to two types of spike-train inputs. Two characteristic quantities can be extracted, which can reflect partially the dynamical process of a HH neuron.
The Arabidopsis pentatricopeptide repeat protein PDM1 is associated with the intergenic sequence of S11-rpoA for rpoA monocistronic RNA cleavage
QianQian Yin,YongLan Cui,GuoRui Zhang,HongDao Zhang,XiaoMeng Wang,ZhongNan Yang
Chinese Science Bulletin , 2012, DOI: 10.1007/s11434-012-5278-9
Abstract: The PDM1 gene encodes a pentatricopeptide repeat protein of the PLS subfamily. It is essential for rpoA polycistronic processing in Arabidopsis. In this study, we performed functional analysis of PDM1 in rpoA monocistronic cleavage and chloroplast development. The pdm1 mutants display an albino lethal phenotype with severe chloroplast development defects. When the construct of PDM1 fused with the GFP gene was introduced into Arabidopsis protoplasts, the GFP signal was exclusively observed in chloroplasts. This shows that PDM1 is localized to chloroplast. In the wild type, the rpoA transcript of about 990 nt is processed. In pdm1, this transcript is absent. However, Western blot showed that the RpoA protein in pdm1 is accumulated at a level approximately 1/3 of the wild type. This suggests that certain other transcripts processed from L23-L2-S19-L22-S3-L16-L14-S8-L36-S11-rpoA polycistronic precursor may be used as templates for protein translation. To determine whether PDM1 can bind to the rpoA pre-mRNA, we generated a transgenic Arabidopsis line with PDM1 fused to FLAG tag that was capable of complementing the pdm1-1 mutant phenotype. RNA immunoprecipitation analysis showed that PDM1 is associated with S11-rpoA intergenic sequence. This indicates that PDM1 may bind to the S11-rpoA intergenic region to perform rpoA processing.
AtMYB103 is a crucial regulator of several pathways affecting Arabidopsis anther development
Jun Zhu,GuoQiang Zhang,YuHua Chang,XiaoChuan Li,Jun Yang,XueYong Huang,QingBo Yu,Hui Chen,TianLong Wu,ZhongNan Yang
Science China Life Sciences , 2010, DOI: 10.1007/s11427-010-4060-y
Abstract: Previous reports indicated that AtMYB103 has an important role in tapetum development, callose dissolution, and exine formation in A. thaliana anthers. Here, we further characterized its function in anther development by expression pattern analysis, transmission electron microscopy observation of the knockout mutant, and microarray analysis of downstream genes. A total of 818 genes differentially expressed between ms188 and the wild-type were identified by global expression profiling analysis. Functional classification showed that loss-of-function of AtMYB103 impairs cell wall modification, lipid metabolic pathways, and signal transduction throughout anther development. RNA in situ hybridization confirmed that transcription factors acting downstream of AtMYB103 (At1g06280 and At1g02040) were expressed in the tapetum and microspores at later stages, suggesting that they might have important roles in microsporogenesis. These results indicated that AtMYB103 is a crucial regulator of Arabidopsis anther development.
Functional Analysis of OsMS2 Gene Involved in Anther Development of Rice

Zijun Fang,Qilong Shi,Zhongnan Yang,Sen Zhang,

植物学报 , 2008,
Abstract: 拟南芥MS2(MALE STERILITY 2)是一个调控花药花粉发育的关键基因。水稻OsMS2(Os03g07140)基因与拟南芥MS2的序列具有高度同源性。利用RNA干扰技术研究OsMS2基因在水稻花药发育过程中的功能。与野生型水稻相比, 转基因 植株营养生长阶段正常, 但雄性育性降低。转基因植株雄性育性降低与RNA干扰引起的OsMS2基因表达水平降低有关。进一步对转基因植株花药进行细胞学观察, 结果表明OsMS2基因表达水平的降低导致绒毡层细胞退化延迟, 小孢子壁的形成出现异常。扫描电镜观察结果显示, 小孢子壁光滑, 不能形成正常的外壁。以上结果表明OsMS2基因在水稻花药发育过程中起重要作用。
Study of Subcellular Localization of the Expressed Protein in Arabidopsis thaliana

Pengcheng Wang,Chen Wang,Hualing Mi,Genyu Zhou,Zhongnan Yang,

植物学报 , 2006,
Abstract: 生物信息学分析表明, 模式植物拟南芥叶绿体中含有大约4 000多种蛋白质, 目前只分离得到1 000多种, 其他预测的叶绿体蛋白的实验验证对叶绿体功能研究有重要意义。本文对一个预测的叶绿体未知功能蛋白AT5G48790进行了亚细胞定位研究。我们克隆了该基因5'端长178 bp的DNA片段, 与绿色荧光蛋白(GFP)基因构建重组载体pMON530-cTP-GFP。转基因植株通过激光共聚焦显微镜观察, GFP只在叶绿体中特异表达。实验结果表明, AT5G48790的确为叶绿体蛋白。本实验方法也可用于其他预测的蛋白质的实验验证。
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