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Search Results: 1 - 10 of 4940 matches for " Zhibing Lai "
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Roles of Arabidopsis WRKY3 and WRKY4 Transcription Factors in Plant Responses to Pathogens
Zhibing Lai, KM Vinod, Zuyu Zheng, Baofang Fan, Zhixiang Chen
BMC Plant Biology , 2008, DOI: 10.1186/1471-2229-8-68
Abstract: Both WRKY3 and WRKY4 are nuclear-localized and specifically recognize the TTGACC W-box sequences in vitro. Expression of WRKY3 and WRKY4 was induced rapidly by stress conditions generated by liquid infiltration or spraying. Stress-induced expression of WRKY4 was further elevated by pathogen infection and SA treatment. To determine directly their role in plant disease resistance, we have isolated T-DNA insertion mutants and generated transgenic overexpression lines for WRKY3 and WRKY4. Both the loss-of-function mutants and transgenic overexpression lines were examined for responses to the biotrophic bacterial pathogen Pseudomonas syringae and the necrotrophic fungal pathogen Botrytis cinerea. The wrky3 and wrky4 single and double mutants exhibited more severe disease symptoms and support higher fungal growth than wild-type plants after Botrytis infection. Although disruption of WRKY3 and WRKY4 did not have a major effect on plant response to P. syringae, overexpression of WRKY4 greatly enhanced plant susceptibility to the bacterial pathogen and suppressed pathogen-induced PR1 gene expression.The nuclear localization and sequence-specific DNA-binding activity support that WRKY3 and WRKY4 function as transcription factors. Functional analysis based on T-DNA insertion mutants and transgenic overexpression lines indicates that WRKY3 and WRKY4 have a positive role in plant resistance to necrotrophic pathogens and WRKY4 has a negative effect on plant resistance to biotrophic pathogens.Upon pathogen infection, pathogen-associated molecular patterns (PAMPs) such as bacterial flagellin and lipopolysaccharides are recognized by plant receptors to activate PAMP-triggered immunity through a mitogen-activated protein kinase signaling cascade [1]. Gram-negative bacterial pathogens such as Pseudomonas syringae can deliver effector proteins to plant cells to interfere PAMP-triggered resistance to promote pathogen virulence. As a result, the remaining basal defense is usually insuffi
Roles of arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress
Han Chen, Zhibing Lai, Junwei Shi, Yong Xiao, Zhixiang Chen, Xinping Xu
BMC Plant Biology , 2010, DOI: 10.1186/1471-2229-10-281
Abstract: We report that the three WRKYs are involved in plant responses to abscisic acid (ABA) and abiotic stress. Through analysis of single, double, and triple mutants and overexpression lines for the WRKY genes, we have shown that WRKY18 and WRKY60 have a positive effect on plant ABA sensitivity for inhibition of seed germination and root growth. The same two WRKY genes also enhance plant sensitivity to salt and osmotic stress. WRKY40, on the other hand, antagonizes WRKY18 and WRKY60 in the effect on plant sensitivity to ABA and abiotic stress in germination and growth assays. Both WRKY18 and WRKY40 are rapidly induced by ABA, while induction of WRKY60 by ABA is delayed. ABA-inducible expression of WRKY60 is almost completely abolished in the wrky18 and wrky40 mutants. WRKY18 and WRKY40 recognize a cluster of W-box sequences in the WRKY60 promoter and activate WRKY60 expression in protoplasts. Thus, WRKY60 might be a direct target gene of WRKY18 and WRKY40 in ABA signaling. Using a stable transgenic reporter/effector system, we have shown that both WRKY18 and WRKY60 act as weak transcriptional activators while WRKY40 is a transcriptional repressor in plant cells.We propose that the three related WRKY transcription factors form a highly interacting regulatory network that modulates gene expression in both plant defense and stress responses by acting as either transcription activator or repressor.Plants are constantly exposed to a variety of biotic and abiotic stresses and have evolved intricate mechanisms to sense and respond to the adverse conditions. Phytohormones such as salicylic acid (SA), ethylene (ET), jasmonic acid (JA) and abscisic acid (ABA) play important roles in the regulation of plant responses to the adverse environmental conditions. In Arabidopsis, mutants deficient in SA biosynthesis (e.g. sid2) or signalling (e.g. npr1) exhibit enhanced susceptibility to biotrophic pathogens, which parasitize on plant living tissue [1,2]. ET- and JA-mediated signaling pat
A first genome assembly of the barley fungal pathogen Pyrenophora teres f. teres
Simon R Ellwood, Zhaohui Liu, Rob A Syme, Zhibing Lai, James K Hane, Felicity Keiper, Caroline S Moffat, Richard P Oliver, Timothy L Friesen
Genome Biology , 2010, DOI: 10.1186/gb-2010-11-11-r109
Abstract: The total assembly was 41.95 Mbp and contains 11,799 gene models of 50 amino acids or more. Comparison against two sequenced BACs showed that complex regions with a high GC content assembled effectively. Electrophoretic karyotyping showed distinct chromosomal polymorphisms between isolates 0-1 and 15A, and cytological karyotyping confirmed the presence of at least nine chromosomes. The genetic map spans 2477.7 cM and is composed of 243 markers in 25 linkage groups, and incorporates simple sequence repeat markers developed from the assembly. Among predicted genes, non-ribosomal peptide synthetases and efflux pumps in particular appear to have undergone a P. teres f. teres-specific expansion of non-orthologous gene families.This study demonstrates that paired-end Solexa sequencing can successfully capture coding regions of a filamentous fungal genome. The assembly contains a plethora of predicted genes that have been implicated in a necrotrophic lifestyle and pathogenicity and presents a significant resource for examining the bases for P. teres f. teres pathogenicity.Net blotch of barley (Hordeum vulgare) is caused by Pyrenophora teres Drechsler (anamorph Drechslera teres [Sacc.] Shoem.). P. teres is an ascomycete within the class Dothideomycetes and order Pleosporales. This order contains plant pathogens responsible for many necrotrophic diseases in crops, including members of the genera Ascochyta, Cochliobolus, Pyrenophora, Leptosphaeria and Stagonospora. Net blotch is a major disease worldwide that causes barley yield losses of 10 to 40%, although complete loss can occur with susceptible cultivars in the absence of fungicide treatment [1]. In Australia the value of disease control is estimated at $246 million annually with average direct costs of $62 million annually, making it the country's most significant barley disease [2].Net blotch exists in two morphologically indistinguishable but genetically differentiated forms: P. teres f. teres (net form of net blotch,
Cloning and Characterization of a Xanthomonadin Gene Cluster from Xanthomoinas oryzae pv. oryzicola

Zou Huasong,Chen Gongyou,Lai Zhibing,Li Guangyong,Wang Jinsheng,

微生物学报 , 2003,
Abstract: A novel gene pig H had been identified from Xanthomonas oryzae pv. oryzae(Xoo) gDNA library clone pA341 previously. This paper reports a xanthomonadin biosynthetic ( pig ) gene cluster from Xanthomonas oryzae pv. oryzicola(Xooc) . Five pigment minus mutants from Xoo and thirteen from Xooc were obtained by diethyl sulfate (DES) mutagensis. The mutants M6 and M12 failed to cause disease in rice and induce the HR in tobacco either. One clone pA270 was screened from Xooc RS105 gDNA library after all individual clone was transferred into a mutant M1071 Clony blot hybridization displayed that the foreign NDA fragment harbored in pA341 was not homologous to that in pA270 Moreover, only 10 of the above 18 xanthomonadin mutants were restored to produce xanthomonadin by either pA341 or pA270 clone. This demonstrated that the mutated sites in the above 18 xanthomonadin were different and might not be in a same gene locus. Subclones of a 11 6kb Eco RI fragment affected either the amount or absorption spectra of pigment production, which indicated that this fragment encoded various functional fragments and was possibly a gene cluster. The roles of the pigment in protecting the bacterial cells from damage by UV radiation suggested that the pigment contribute to the survival of the pathogens during their colonization in rice.
1H-NMR-Based Metabolic Analysis of Human Serum Reveals Novel Markers of Myocardial Energy Expenditure in Heart Failure Patients
Zhiyong Du, Anna Shen, Yuli Huang, Liang Su, Wenyan Lai, Peng Wang, Zhibing Xie, Zhiquan Xie, Qingchun Zeng, Hao Ren, Dingli Xu
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0088102
Abstract: Objective Elevated myocardial energy expenditure (MEE) is related with reduced left ventricular ejection fraction, and has also been documented as an independent predictor of cardiovascular mortality. However, the serum small-molecule metabolite profiles and pathophysiological mechanisms of elevated MEE in heart failure (HF) are still lacking. Herein, we used 1H-NMR-based metabolomics analysis to screen for potential biomarkers of MEE in HF. Methods A total of 61 subjects were enrolled, including 46 patients with heart failure and 15 age-matched controls. Venous serum samples were collected from subjects after an 8-hour fast. An INOVA 600 MHz nuclear magnetic resonance spectrometer with Carr-Purcell-Melboom-Gill (CPMG) pulse sequence was employed for the metabolomics analysis and MEE was calculated using colored Doppler echocardiography. Metabolomics data were processed using orthogonal signal correction and regression analysis was performed using the partial least squares method. Results The mean MEE levels of HF patients and controls were 139.61±58.18 cal/min and 61.09±23.54 cal/min, respectively. Serum metabolomics varied with MEE changed, and 3-hydroxybutyrate, acetone and succinate were significantly elevated with the increasing MEE. Importantly, these three metabolites were independent of administration of angiotensin converting enzyme inhibitor, β-receptor blockers, diuretics and statins (P>0.05). Conclusions These results suggested that in patients with heart failure, MEE elevation was associated with significant changes in serum metabolomics profiles, especially the concentration of 3-hydroxybutyrate, acetone and succinate. These compounds could be used as potential serum biomarkers to study myocardial energy mechanism in HF patients.
Logistics and Regional Economic Growth - Evidence from the Space Econometric Panel Data from China
Zhibing Cai,Jianchu Zhou
Economic Management Journal , 2012,
Abstract: Unlike the traditional economic growth model, spatial contact must be noted when we construct relative models in the study of the relationship between logistics industry development and regional economic. With the use of space panel econometric model and CD production function, by constructing a regional logistics industry development and regional economic growth economic model, the study about the economic growth of China's provinces and the logistics industry during 1988-2009 is carried out. The results show that there are significant spatial correlation between Chinese province's economic growth and development of the logistics industry. 1 percent increase in the neighboring provinces logistics industry will make the province's GDP increase about 0.15 percent. Finally, according to estimations, some relevant policy recommendations are given.
Transient of the kinetic spherical model between two temperatures
Chunshan He,Zhibing Li
Physics , 2003,
Abstract: We solve the dynamic equation for the kinetic spherical model that initially is in an arbitrary equilibrium state and then is left to evolve in a heat-bath with another temperature. Flows of the Renormalizational group are determined.
Graphene with the secondary amine-terminated zigzag edge as a line electron emitter
Weiliang Wang,Zhibing Li
Physics , 2012, DOI: 10.1007/s00339-012-7180-3
Abstract: An extraordinary low vacuum barrier height of 2.30 eV has been found on the zigzag-edge of graphene terminated with the secondary amine via the ab initio calculation. This edge structure has a flat band of edge states attached to the gamma point where the transversal kinetic energy is vanishing. We show that the field electron emission is dominated by the flat band. The edge states pin the Fermi level to a constant, leading to an extremely narrow emission energy width. The graphene with such edge is a promising line field electron emitter that can produce highly coherent emission current.
Comment on slope of nonlinear FN plot and field enhancement factor
Weiliang Wang,Zhibing Li
Physics , 2012,
Abstract: It is common practice to extract field enhancement factor from the slope of FN plot. Many experimentalists working on field electron emission had reported multi-(linear segment) FN plots, which can be divided into several (usually two) linear segments. Then multi-(field enhancement factor) were extracted from the FN plot. They claimed that the field enhancement factor increases with applied field if the FN plot bends downward (vice versus if the FN plot bends upward). We show that this is contrary to fact.
Characteristic frequency of the magnetic radiation of spinor condensates
Zhibing Li,Chengguang Bao
Physics , 2007, DOI: 10.1103/PhysRevA.75.063606
Abstract: The magnetic radiation of the fully-condensed states of $^{23}$Na condensates have been studied. A narrow characteristic spectral line with a wave length proportional to N^(-2/5) (N is the number of particles) and with a probability of transition proportional to N^(17/5) emitted (absorbed) by the condensate was found. It implies that short wave radiation with a huge probability of transition can be obtained if numerous atoms are trapped. A new technique developed by the authors, namely, the analytical forms of the fractional parentage coefficients, was used to calculate analytically the matrix elements between the total spin-states.
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