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Search Results: 1 - 10 of 65299 matches for " Zhi-kui WU "
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Treatment of β-thalassemia with Bushen Yisui therapy: a randomized controlled trial
Zhi-kui WU
Zhong Xi Yi Jie He Xue Bao , 2007,
Abstract: Objective: To investigate the efficacy and safety of Yisui Shengxue Granule (YSSXG), a compound traditional Chinese herbal medicine, in treating β-thalassemia. Methods: A randomized single-blinded trial was designed. Sixty patients with β-thalassemia were divided into two groups: 30 patients in YSSXG-treated group and 30 in placebo parallel-control group. The patients in the two groups were assigned to receive either YSSXG or placebo for three months. The patients' symptoms and their blood indexes such as hemoglobin (Hb), red blood cell (RBC), reticulocytes (Ret) and fetal hemoglobin (HBF) were examined before and after the treatment. Meanwhile, the liver and spleen were examined with B-mode ultrasound. Results: In the YSSXG-treated group, the blood indexes (Hb, RBC, Ret and HBF) and the symptoms of the patients were improved after three-month treatment, with statistical significance compared to those before treatment (P<0.01); hepatauxe and splenomegaly were also relieved (P<0.05) and no adverse reactions were monitored. In the placebo parallel-control group, no significant improvement of the blood indexes and symptoms, as well as the hepatauxe and splenomegaly had been found (P>0.05). Conclusion: YSSXG demonstrates obvious clinical efficacy and no adverse reactions in treating β-thalassemia.
Clinical observation of Yisui Shengxue Granule in treating 25 patients with hemoglobin H disease
Wen-juan WANG,Zhi-kui WU
Zhong Xi Yi Jie He Xue Bao , 2008,
Abstract: Objective: To investigate the clinical efficacy and safety of Yisui Shengxue Granule (YSSXG), a compound traditional Chinese herbal medicine for reinforcing kidney and nourishing blood, in treating hemoglobin H (HbH) disease.Methods: YSSXG was given orally to 25 patients with HbH disease in Guangxi Zhuang Autonomous Region (high incidence area for HbH disease in China) for 3 months as one therapeutic course, 3 times a day, 10 g YSSXG was given each time (dose of YSSXG for children should be reduced properly), and blood transfusion was not given to HbH patients during the course of treatment. The levels of hemoglobin (Hb), red blood cell (RBC), HbH and reticulocyte (Ret) were observed before and after YSSXG treatment, and side effects were observed during the course of treatment. Meanwhile, the genotype was examined, and the clinical efficacy of YSSXG in treating HbH patients with different genotype was evaluated.Results: The levels of Hb, RBC and Ret were obviously increased after YSSXG treatment from the first month to the end of treatment (P<0.01). After YSSXG treatment, the levels of Hb, RBC, Ret in 12 HbH patients with gene deletion were elevated (P<0.05, P<0.01), and the levels of Hb and Ret in 13 HbH patients with gene non-deletion were increased obviously (P<0.05, P<0.01). The total response rate was 84% after 3-month treatment, and there was no statistical difference in clinical efficacy between gene deletion HbH patients and non-deletion HbH patients. No adverse effect was observed during the course of treatment.Conclusion: YSSXG is effective and safe for treatment of HbH disease. YSSXG can improve the levels of Hb, RBC and Ret in HbH patients, especially in gene deletion HbH patients.
Effects of interstitial chemotherapy using thermosensitive gel-coated ricin on hepatoma H22-bearing mice
Zhi-kui CHEN,Li-wu LIN
Zhong Xi Yi Jie He Xue Bao , 2009,
Abstract: Objective: To explore the efficacy and feasibility of interstitial chemotherapy using thermosensitive gel-coated ricin in hepatoma H22-bearing mice.Methods: Ricin was purified by chromatography method. The purified ricin was identified by Western blot assay and the purity was determined by high-performance liquid chromatography. BALB/c mice were inoculated subcutaneously in right flank with hepatoma H22 cells. When the tumor size reached about 1.0 cm in diameter, 40 mice were randomly divided into untreated group, thermosensitive gel group, ricin group and thermosensitive gel-coated ricin group. Mice in each group were administered different agents by percutaneous intratumoral injection, including normal saline, thermosensitive hydrogel, ricin and thermosensitive gel-coated ricin. Fifteen days after treatment, the tumors were removed to calculate inhibition rate of tumor growth. The tumor tissues were made into pathological sections to perform histopathological examination. The ultrastructure of tumor tissue was examined by electron microscope examination as well. Blood was collected to detect the hepatic and renal functions. The caspase-3 activity of tumor tissue was determined by using zymologic method with a spectrophotometer.Results: After intratumoral therapy, tumor weight in the thermosensitive gel-coated ricin group was lower than that in the untreated group, with a tumor growth inhibition rate of 71.31%. No obvious hepatic or renal toxicities were detected after thermosensitive gel-coated ricin treatment. Histopathologic observation of the tumor tissue showed massive necrosis and typical apoptosis phenomena, including chromatin margination and apoptotic body. Meanwhile, thermosensitive gel-coated ricin resulted in a significant increase in the caspase-3 activity as compared with the untreated group and the ricin group (P<0.01, P<0.05).Conclusion: The above findings indicate that intratumoral therapy with thermosensitive gel-coated ricin has strong antitumor effect and can obviously lessen systemic toxicity, which may provide an effective and feasible method for hepatocellular carcinoma treatment.
A family survey of syndromes of traditional Chinese medicine in patients with β-thalassemia
Wen-juan WANG,Zhi-kui WU
Zhong Xi Yi Jie He Xue Bao , 2009,
Abstract: Objective: To explore the relationship between syndromes of traditional Chinese medicine (TCM) and genetic background in patients with β-thalassemia.Methods: TCM syndromes were surveyed in the selected 78 patients with β-thalassemia intermedia including 120 parents. The gene mutations were detected separately. The frequency and score of TCM syndromes between the offspring and their parents in different family types were analyzed, and the differences were compared.Results: The 73 families were divided into two family types by hereditary characteristics. Family type one meant that genotypes of one of the parents were normal, while the offspring genotypes were heterozygous and were exactly the same as another parent. In the 22 families of type one, the heterozygous offspring manifested 6 high-frequency symptoms and signs such as spontaneous perspiration, dry mouth and dry throat, pale or sallow complexion, tidal fever and night sweating, lassitude and pale fingernails. The heterozygous parents manifested 5 high-frequency symptoms and signs such as lassitude in loins and knees, dizziness, aversion to cold and cold limbs, tinnitus, dry mouth and dry throat. The normal parents manifested 3 high-frequency symptoms and signs such as lassitude in loins and knees, dizziness, and spontaneous perspiration. TCM syndrome score in the heterozygous offspring was higher than that in the heterozygous and normal parents, but there was no significant difference (P>0.05). Family type two meant that genotypes of both parents were heterozygous, while the offspring genotypes were heterogenic duplex heterozygotes. In the 51 families of type two, the offspring manifested 9 high-frequency symptoms and signs such as pale or sallow complexion, spontaneous perspiration, dry mouth and dry throat, pale fingernails, tidal fever and night sweating, lassitude, frequent attack of common cold, dysphoria with feverish sensation in chest, and yellow discoloration of the skin and sclera. The parents manifested 3 high-frequency symptoms and signs such as lassitude in loins and knees, dizziness, aversion to cold and cold limbs. TCM syndrome score in the offspring was significant higher than that in the parents (P<0.01).Conclusion: In the two family types, TCM syndrome in the offspring is of yin-blood deficiency, while the syndrome of the parents is of kidney deficiency. The differences of TCM syndromes between the offspring and the parents may have some relations to the type of mutant genes and genetically modified ingredients. This research provides scientific evidence to TCM syndrome differen
Eos Negatively Regulates Human γ-globin Gene Transcription during Erythroid Differentiation
Hai-Chuan Yu, Hua-Lu Zhao, Zhi-Kui Wu, Jun-Wu Zhang
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0022907
Abstract: Background Human globin gene expression is precisely regulated by a complicated network of transcription factors and chromatin modifying activities during development and erythropoiesis. Eos (Ikaros family zinc finger 4, IKZF4), a member of the zinc finger transcription factor Ikaros family, plays a pivotal role as a repressor of gene expression. The aim of this study was to examine the role of Eos in globin gene regulation. Methodology/Principal Findings Western blot and quantitative real-time PCR detected a gradual decrease in Eos expression during erythroid differentiation of hemin-induced K562 cells and Epo-induced CD34+ hematopoietic stem/progenitor cells (HPCs). DNA transfection and lentivirus-mediated gene transfer demonstrated that the enforced expression of Eos significantly represses the expression of γ-globin, but not other globin genes, in K562 cells and CD34+ HPCs. Consistent with a direct role of Eos in globin gene regulation, chromatin immunoprecipitaion and dual-luciferase reporter assays identified three discrete sites located in the DNase I hypersensitivity site 3 (HS3) of the β-globin locus control region (LCR), the promoter regions of the Gγ- and Aγ- globin genes, as functional binding sites of Eos protein. A chromosome conformation capture (3C) assay indicated that Eos may repress the interaction between the LCR and the γ-globin gene promoter. In addition, erythroid differentiation was inhibited by enforced expression of Eos in K562 cells and CD34+ HPCs. Conclusions/Significance Our results demonstrate that Eos plays an important role in the transcriptional regulation of the γ-globin gene during erythroid differentiation.
Serotonin Influences the Endogenous Opiate Peptides in the Rat Spinal Cord to Participates in Pain Modulation  [PDF]
Yan-Juan Pan, Zhi-Kui Yin, Jun Yang, Ying Zhao, Da-Xin Wang
Open Journal of Anesthesiology (OJAnes) , 2012, DOI: 10.4236/ojanes.2012.22008
Abstract: Spinal cord is a necessary pathway that transfers the body nociceptive inputs to the brain. Endogenous opiate peptides have been proven to participate in the nociceptive process at spinal level. It has reported that serotonin (5-HT, 5-hydroxytryptamine) in spinal cord plays a role in pan modulation, which can be blocked by opiate receptor antagonists. The present study was designed to investigate the interaction between 5-HT and endogenous opiate peptides at rat spinal level effecting on pain modulation. The results showed that 1) pain stimulation increased not only leucine-enkephalin (L-Ek), β-endorphin (β-Ep) and dynorphin A1-13 (DynA1-13) concentrations but also 5-HT and 5-hydorxyindoleace acid (5-HIAA, the 5-HT main metabolic product) concentrations in spinal cord significantly; 2) 5-HT could increase L-Ek, β-Ep and DynA1-13 concentrations in spinal cord in a dose-dependent manner, whereas cypotolamine (a 5-HT receptor antagonist) decreased L-Ek, β-Ep and DynA1-13 concentrations in spinal cord. The data suggested that 5-HT antinociceptive role might be involved in the endogenous opiate peptide system through 5-HT receptors at spinal level.
The value of Ultrasound in establishing rabbit model bearing VX2 liver Tumor

WANG Yan,LIN Li-wu,CHEN Zhi-kui,LIN Xiao-dong,LIN Zhen-hu,YU Li-yun,LIANG Rong-xi,

中华医学超声杂志(电子版) , 2009,
Abstract: 目的 探讨开腹直视瘤块接种法及超声引导下肿瘤单细胞悬液接种法制作兔VX2原位肝癌模型的特点.方法 选择新西兰大白兔16只,随机分为开腹组及超声介入组,每组8只;分别在开腹直视下将瘤粒接种于兔肝内及超声引导下将单细胞悬液注入肝内,1周后间断行超声检查,动态观察肿瘤生长情况及有无并发症等,并对两组动物超声与病理检查结果进行对比分析,评价超声在兔VX2原位肝癌模型制作中的应用价值开腹组与超声介入组原位种植分别为7只与6只,无明显差异.虽然开腹组平均成瘤时间早于超声介入组,但是超声介入组实验操作简单、省时,且并发症明显少于开腹组,分别是1只(超声介入组)和7只(开腹组).接种后期(>28 d)均出现肝内转移和远处转移,分别为8个(超声介入组)和14个(开腹组)转移灶.且可以用超声实时监测肿瘤的大小、形态、回声、血流情况等.结论 超声引导接种制作的兔VX2原位肝癌模型是超声介入治疗研究的理想动物模型,超声检查可作为监测兔VX2原位肝癌模型的一种简便准确的影像学方法.
Cofactor engineering through heterologous expression of an NADH oxidase and its impact on metabolic flux redistribution in Klebsiella pneumoniae
Ji Xiao-Jun,Xia Zhi-Fang,Fu Ning-Hua,Nie Zhi-Kui
Biotechnology for Biofuels , 2013, DOI: 10.1186/1754-6834-6-7
Abstract: Background Acetoin is an important bio-based platform chemical. However, it is usually existed as a minor byproduct of 2,3-butanediol fermentation in bacteria. Results The present study reports introducing an exogenous NAD+ regeneration sysytem into a 2,3-butanediol producing strain Klebsiella pneumoniae to increse the accumulation of acetoin. Batch fermentation suggested that heterologous expression of the NADH oxidase in K. pneumoniae resulted in large decreases in the intracellular NADH concentration (1.4 fold) and NADH/NAD+ ratio (2.0 fold). Metabolic flux analysis revealed that fluxes to acetoin and acetic acid were enhanced, whereas, production of lactic acid and ethanol were decreased, with the accumualation of 2,3-butanediol nearly unaltered. By fed-batch culture of the recombinant, the highest reported acetoin production level (25.9 g/L) by Klebsiella species was obtained. Conclusions The present study indicates that microbial production of acetoin could be improved by decreasing the intracellular NADH/NAD+ ratio in K. pneumoniae. It demonstrated that the cofactor engineering method, which is by manipulating the level of intracellular cofactors to redirect cellular metabolism, could be employed to achieve a high efficiency of producing the NAD+-dependent microbial metabolite.
Design and implementation of embedded digital mapping based on OOP

LONG Bin,ZHOU Zhi-kui,

重庆邮电大学学报(自然科学版) , 2005,
Abstract: 从软件设计的角度出发,吸取了目前流行的GIS系统的一些特点,并结合车载导航系统的特殊要 求,分析了如何从底层构造开始来设计与实现基于嵌入式系统的开放式数字地图,同时对主要模块中涉及 的关键技术进行了相应的探讨5该系统充分利用OOP技术,简化了系统的设计,缩短了开发周期.
Analysis of photosynthetic simulation by a biochemical model or mathematical model in greenhouse eggplant
温室茄子(Solanum melongena L.)光合数学模型与光合生化模型模拟分析

GAO Zhi-Kui,GAO Rong-Fu,HE Jun-Ping,WANG Mei,ZHONG Chuan-Fei,

生态学报 , 2007,
Abstract: 针对植物光合与内外环境因子间的关系以及光合“午睡”现象中的气孔限制与非气孔限制问题,以温室茄子‘茄杂一号’为试材,对叶室温光组合方式下测定的净光合速率Pn对胞间CO2浓度Ci响应曲线,和人工增施CO2处理下测定的Pn日变化进程,进行了光合数学模型和Farquhar、von Caemmerer和Berry的光合生化动力学模型(简称为FvCB模型)模拟分析。采用美国思爱迪生态仪器有限公司的CI-301PS光合作用测定仪进行净光合速率(Pn)、光合有效辐射(PAR)、气温(Ta)、叶温(Tl)、环境二氧化碳浓度(Ca)、胞间二氧化碳浓度(Ci)和空气相对湿度(Hr)参数测定。其结果表明,无论是Pn对Ci的响应曲线还是光合日进程中,数学模型对Pn的拟合度明显优于为FvCB模型。因此,通过数学模型可以解析出光合日进程受单一环境因子(PAR、Ta、Ca、Hr)及其复合环境因子的综合影响。然而,FvCB模型模拟结果显示出,温光组合下受Rubisco(即RuBP羧化/加氧酶)数量与活性及动力学特性限制的羧化速率Ac、受RuBP(1,5-二磷酸核酮糖)再生限制的羧化速率Aj以及受TPU(磷酸丙糖)可利用量限制的羧化速率Ap对Ci响应的主控作用呈现交替变化趋势。其交替变化转折点胞间二氧化碳浓度Cicj在强光高温组合中较高,而在弱光低温组合中较低;同时还发现,Cicj和Cijp受叶温的影响强于光照。光合日进程中的FvCB模型模拟分析揭示出,早晨和傍晚弱光下为Aj限制时段;晴天上午和中午前后的充足日照下为Ac限制时段。多云和阴天下Aj的限制时段延长。增施CO2会延长Aj的限制时段,同时相应缩短Ac的限制时段;冬季2次增施CO2的出现了Ap限制时段。
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