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Search Results: 1 - 10 of 104322 matches for " Zhenjun Zhang "
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A two-dimensional electrophoresis reference map of the earthworm Eisenia fetida  [PDF]
Xing Wang, Yi Zhang, Zhenjun Sun
Natural Science (NS) , 2012, DOI: 10.4236/ns.2012.411115
Abstract: The molecular mechanisms underlying innate immunity in the earthworm E. fetida remain unclear. For the recognition of innate immunity in the earthworm E. fetida, a detailed knowledge of this proteome is a prerequisite. The absence of a high-resolution E. fetida proteome map prompted us to determine E. fetida protein spots that can be visualised on 2-D protein gels. In this study, we present a preliminary description of the whole earthworm E. fetida proteome. A highly detailed two-dimensional gel electrophoresis (2-DE) map of the E. fetida proteome was established and approximately 1500 protein spots were detected from the earthworm sample when applying a 500 μg protein 2-DE in the pH range 3.0 - 10.0. We present a 2-DE proteome map of E. fetida, identifying 76 different proteins by matrix-assisted laser desorption/ionization-tandem time of flight mass spectrometry (MALDI-TOF/ TOF-MS) analysis. These identified proteins, including heat shock protein 90 (Hsp90), chaperonine protein HSP60, caspase-8, fibrinolytic protease 0, gelsolin-like protein, lombricine kinase, coelomic cytolytic factor1 (CCF 1), manganous superoxide dismutase (MnSOD), triosephosphate isomerase, extracellular globin-4, lysenin, and intermediate filament protein, glyceraldehyde-3- phosphate dehydrogenase, et al., are involved in several processes, including transcripttion, translation, the tricarboxylic acid cycle, the cellular amino acid metabolic process, protein amino acid phosphorylation, glycolysis, and the glucose metabolic process. These 2-DE data will enhance future comparisons of immunity, toxicology, biotic processes and other challenges, thereby allowing for further study of the molecular mechanisms in response to environmental stressors, and it will be useful to investigate environmental proteomics in invertebrate earthworms.
Secure Image Encryption without Size Limitation Using Arnold Transform and Random Strategies
Zhenjun Tang,Xianquan Zhang
Journal of Multimedia , 2011, DOI: 10.4304/jmm.6.2.202-206
Abstract: Encryption is an efficient way to protect the contents of digital media. Arnold transform is a significant technique of image encryption, but has weaknesses in security and applications to images of any size. To solve these problems, we propose an image encryption scheme using Arnold transform and random strategies. It is achieved by dividing the image into random overlapping square blocks, generating random iterative numbers and random encryption order, and scrambling pixels of each block using Arnold transform. Experimental results show that the proposed encryption scheme is robust and secure. It has no size limitation, indicating the application to any size images.
PCR-DGGE analysis of earthworm gut bacteria diversity in stress of Escherichia coli O157:H7  [PDF]
Yi Zhang, Gaochan Wang, Yupeng Wu, Hui Zhao, Yufeng Zhang, Zhenjun Sun
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.43A058
Abstract:

In order to test if the intestinal bacteria play an important role in antibacterial ability of earthworm, we chose Escherichia coli O157:H7, an anthropozoonosis pathogen, as a biological stressor and studied the change of intestinal bacteria community of earthworm by PCR-DGGE analysis. Results showed that the pathogen merely existed 1 - 3 days, then almost disappeared after through the earthworm’s gut. In this period, the diversity and abundance index of intestinal bacteria increased first, then decreased, and finally kept stably after 7 days. The result demonstrated that the intestinal bacteria of earthworm had ability of adjust community structure to eliminate the pathogen E. coli O157:H7, and the amount of bacteria Bacillus increased significantly, which might be the positive antagonism to E. coli O157:H7.

Branching ratios and CP-violating asymmetries of $B_s \to h_1 h_2 $ decays in the general two-Higgs doublet models
Dong Zhang,Zhenjun Xiao,Chong Sheng Li
Physics , 2000, DOI: 10.1103/PhysRevD.64.014014
Abstract: Based on the low-energy effective Hamiltonian with the generalized factorization, we calculate the new physics contributions to branching ratios and CP-violating asymmetries of the charmless hadronic decays $B_s \to h_1 h_2$ in the standard model and the general two-Higgs doublet models (models I, II, and III). Within the considered paramter space, we find the following. (a) In models I and II, the new physics corrections are always small in size and will be masked by other larger known theoretical uncertainties. (b) In model III, the new physics corrections to the branching ratios of those QCD penguin-dominated decays $\ov B_s \to K^0\etapp, K^+ K^{-*}, etc.$, are large in size and insensitive to the variations of $\mhp$ and $\nceff$. For tree- or electroweak penguin-dominated decay modes, however, the new physics corrections are very small in size. (c) For $\ov B_s \to K^+ K^{-*}$ and other seven decay modes, the branching ratios are at the level of $(1-3)\times 10^{-5}$ and will be measurable at the future hadron colliders with large $b$ production. (d) Among the studied thirty nine $B_s$ meson decay modes, seven of them can have a CP-violating asymmetry ${\cal A}_{CP}$ larger than 20% in magnitude. The new physics corrections are small or moderate in magnitude. (e) Because of its large and $\nceff$ stable branching ratio and CP violating asymmetry, the decay $\ov B_s \to K^+ K^{-*}$ seems to be the "best" channel to find CP violation of $B_s$ system through studies of two-body charmless decays of $B_s$ meson.
Recent Advances in Finite Element Applications in Artificial Lumbar Disc Replacement  [PDF]
Zhenjun Zhang, Yitao Sun, Xuejun Sun, Yang Li, Zhenhua Liao, Weiqiang Liu
Journal of Biomedical Science and Engineering (JBiSE) , 2016, DOI: 10.4236/jbise.2016.910B001
Abstract:
As a new choice for the treatment of degenerative lumbar disease, artificial lumbar disc replacement has been widely used in clinical surgery. The finite element is a very effective method to predict and simulate the surgery effect. The purpose of this paper is to review the applications of finite element in artificial lumbar disc replacement, such as design of artificial lumbar disc prosthesis, risk and effect evaluation of artificial lumbar disc replacement, and assessment of operation methods. Lastly, we discuss the future development of finite element method applied in this field, including personalized design of the prosthesis, postoperative behavior guide, and artificial lumbar disc replacement combined with fusion surgery. In conclusion, as an invaluable complement to biomechanical experiments and clinical studies, the finite element method makes important contributions to our understanding of biomechanics of intervertebral disc, and plays an important role in the field of artificial lumbar disc replacement.
The Cognition of Palliative Nursing among Nursing Students at Different Levels after Clinical Practice: A Survey Study  [PDF]
Zhenjun Niu, Hong Zhou
Yangtze Medicine (YM) , 2018, DOI: 10.4236/ym.2018.22008
Abstract: Objective: To understand the status of cognition of palliative nursing among nursing students at different levels, and provide a basis for palliative nursing education. Methods: 268 nursing students from different levels were investigated by the palliative nursing cognition questionnaire and Chinese version of palliative nursing knowledge questionnaire (PCQN). Results: Students with clinical probation and from non single parent family normally have higher knowledge scores than those without clinical probation and from single parent families (p < 0.05); statistical differences were observed in seven aspects of palliative care (p < 0.05); the undergraduates have higher scores, compared to students of junior college and secondary school in dimension two and total score (p < 0.05); the factors include the experience of probation and the grade getting from internship hospital. Conclusion: It’s important for schools and hospitals to strengthen the theory teaching of palliative nursing, combine clinical practice with the theory education closely and improve the teaching level of teachers, which are aimed to promote the rapid development of palliative care.
Ectopic Overexpression of SsCBF1, a CRT/DRE-Binding Factor from the Nightshade Plant Solanum lycopersicoides, Confers Freezing and Salt Tolerance in Transgenic Arabidopsis
Lili Zhang, Zhenjun Li, Jingfu Li, Aoxue Wang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0061810
Abstract: The C-repeat (CRT)/dehydration-responsive element (DRE) binding factor (CBF/DREB1) transcription factors play a key role in cold response. However, the detailed roles of many plant CBFs are far from fully understood. A CBF gene (SsCBF1) was isolated from the cold-hardy plant Solanum lycopersicoides. A subcellular localization study using GFP fusion protein indicated that SsCBF1 is localized in the nucleus. We delimited the SsCBF1 transcriptional activation domain to the C-terminal segment comprising amino acid residues 193–228 (SsCBF1193–228). The expression of SsCBF1 could be dramatically induced by cold, drought and high salinity. Transactivation assays in tobacco leaves revealed that SsCBF1 could specifically bind to the CRT cis-elements in vivo to activate the expression of downstream reporter genes. The ectopic overexpression of SsCBF1 conferred increased freezing and high-salinity tolerance and late flowering phenotype to transgenic Arabidopsis. RNA-sequencing data exhibited that a set of cold and salt stress responsive genes were up-regulated in transgenic Arabidopsis. Our results suggest that SsCBF1 behaves as a typical CBF to contribute to plant freezing tolerance. Increased resistance to high-salinity and late flowering phenotype derived from SsCBF1 OE lines lend more credence to the hypothesis that plant CBFs participate in diverse physiological and biochemical processes related to adverse conditions.
Ectopic Overexpression of SlHsfA3, a Heat Stress Transcription Factor from Tomato, Confers Increased Thermotolerance and Salt Hypersensitivity in Germination in Transgenic Arabidopsis
Zhenjun Li, Lili Zhang, Aoxue Wang, Xiangyang Xu, Jingfu Li
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0054880
Abstract: Plant heat stress transcription factors (Hsfs) are the critical components involved in mediating responses to various environmental stressors. However, the detailed roles of many plant Hsfs are far from fully understood. In this study, an Hsf (SlHsfA3) was isolated from the cultivated tomato (Solanum lycopersicum, Sl) and functionally characterized at the genetic and developmental levels. The nucleus-localized SlHsfA3 was basally and ubiquitously expressed in different plant organs. The expression of SlHsfA3 was induced dramatically by heat stress, moderately by high salinity, and slightly by drought, but was not induced by abscisic acid (ABA). The ectopic overexpression of SlHsfA3 conferred increased thermotolerance and late flowering phenotype to transgenic Arabidopsis plants. Moreover, SlHsfA3 played a negative role in controlling seed germination under salt stress. RNA-sequencing data demonstrated that a number of heat shock proteins (Hsps) and stress-associated genes were induced in Arabidopsis plants overexpressing SlHsfA3. A gel shift experiment and transient expression assays in Nicotiana benthamiana leaves demonstrated that SlHsfA3 directly activates the expression of SlHsp26.1-P and SlHsp21.5-ER. Taken together, our results suggest that SlHsfA3 behaves as a typical Hsf to contribute to plant thermotolerance. The late flowering and seed germination phenotypes and the RNA-seq data derived from SlHsfA3 overexpression lines lend more credence to the hypothesis that plant Hsfs participate in diverse physiological and biochemical processes related to adverse conditions.
TC corrections to the single-top-quark production at the Fermilab Tevatron
Gongru Lu,Yigang Cao,Jinshu Huang,Junde Zhang,Zhenjun Xiao
Physics , 1997,
Abstract: We calculate one-loop corrections to the single-top-quark production via $q\overline{q}' \to t\overline b$ at the Fermilab Tevatron from the Pseudo-Goldstone bosons ( PGBs ) in the framework of one generation technicolor model. The maximum correction to the total cross section for the single-top-quark production is found to reach -2.4% relative to the tree-level cross section, which may be observable at a high-luminosity Tevatron.
Concise Syntheses of Trifluoromethylated Cyclic and Acyclic Analogues of cADPR
Xiangchen Huang,Min Dong,Jian Liu,Kehui Zhang,Zhenjun Yang,Liangren Zhang,Lihe Zhang
Molecules , 2010, DOI: 10.3390/molecules15128689
Abstract: A novel trifluoromethylated analogue of cADPR, 8-CF3-cIDPDE (5) was designed and synthesized via construction of N1,N9-disubstituted hypoxanthine, trifluoromethylation and intramolecular condensation. A series of acyclic analogues of cADPR were also designed and synthesized. These compounds could be useful molecules for studying the structure-activity relationship of cADPR analogues and exploring the cADPR/RyR Ca2+ signalling system.
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