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Search Results: 1 - 10 of 43908 matches for " Zhengli Wu "
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Productivity of crop-fruit ecological agriculture in middle-south Loess Plateau
黄土高原中南部农果复合型生态农业生产力特征

WU Faqi,ZHOU Zhengli,LIU Haibin,
吴发启

应用生态学报 , 2005,
Abstract: With the Xipo,Feimahe and Nangou villages as test objects,the productive characteristics of crop-fruit ecological agriculture in the middle-south Loess Plateau were investigated.The results showed that the biomass productivity of a plant was different with its organs,the highest for grain or fruit,and followed by stem,leaf and root.In the crop-fruit ecological agriculture,the higher ratio the crop subsystem,the higher productivity its biomass,the lower economic productivity and lower economic value was; while the fruit subsystem was on the contrary.The productivity of livestock farming subsystem was in a lower level,which restricted the increase of the total ecosystem's productivity.Based on these results,a countermeasure of increasing the ecosystem productivity was put forward.
Profit and loss of nutrients in crop-fruit ecosystems
农果复合系统营养物质的盈亏平衡特征

WU Faqi,LIU Haibin,ZHOU Zhengli,
吴发启
,刘海斌,周正立

应用生态学报 , 2006,
Abstract: The analysis on the balance of nutrient's profit and loss in crop-fruit ecosystems of three villges in middle-south Loess Plateau showed that fertilization was the main input of nutrients,of which,chemical fertilizer occupied a larger proportion,while organic manure was relatively insufficient.The quantity of N,P and K spent in interior circulation was far less than that exported from the ecosystem.The ratio of nutrient's output and input in three test villages was differed,but all at a low level.
Syntaxin Binding Protein 1 Is Not Required for Allergic Inflammation via IgE-Mediated Mast Cell Activation
Zhengli Wu, Adam J. MacNeil, Jason N. Berman, Tong-Jun Lin
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0058560
Abstract: Mast cells play a central role in both innate and acquired immunity. When activated by IgE-dependent FcεRI cross-linking, mast cells rapidly initiate a signaling cascade and undergo an extensive release of their granule contents, including inflammatory mediators. Some SNARE (soluble N-ethylmaleimide-sensitive fusion factor attachment protein receptor) proteins and SM (Sec1/Munc18) family proteins are involved in mast cell degranulation. However, the function of syntaxin binding protein 1 (STXBP1), a member of SM family, in mast cell degranulation is currently unknown. In this study, we examined the role of STXBP1 in IgE-dependent mast cell activation. Liver-derived mast cells (LMCs) from wild-type and STXBP1-deficient mice were cultured in vitro for the study of mast cell maturation, degranulation, cytokine and chemokine production, as well as MAPK, IκB-NFκB, and NFAT signaling pathways. In addition, in vivo models of passive cutaneous anaphylaxis and late-phase IgE-dependent inflammation were conducted in mast cell deficient Wsh mice that had been reconstituted with wild-type or STXBP1-deficient mast cells. Our findings indicate that STXBP1 is not required for any of these important functional mechanisms in mast cells both in vitro and in vivo. Our results demonstrate that STXBP1 is dispensable during IgE-mediated mast cell activation and in IgE-dependent allergic inflammatory reactions.
Characterization of the duck enteritis virus UL55 protein
Ying Wu, Anchun Cheng, Mingshu Wang, Shunchuan Zhang, Dekang Zhu, Renyong Jia, Qihui Luo, Zhengli Chen, Xiaoyue Chen
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-256
Abstract: The newly identified duck enteritis virus UL55 gene was about 561 bp, it was amplified and digested for construction of a recombinant plasmid pET32a(+)/UL55 for expression in Escherichia coli. SDS-PAGE analysis revealed the recombinant protein UL55(pUL55) was overexpressed in Escherichia coli BL21 host cells after induction by 0.2 mM IPTG at 37°C for 4 h and aggregated as inclusion bodies. The denatured protein about 40 KDa named pUL55 was purified by washing five times, and used to immune rabbits for preparation of polyclonal antibody. The prepared polyclonal antibody against pUL55 was detected and determined by Agar immundiffusion and Neutralization test. The results of Wstern blotting assay and intracellular analysis revealed that pUL55 was expressed most abundantly during the late phase of replication and mainly distributed in cytoplasm in duck enteritis virus infected cells.In this study, the duck enteritis virus UL55 protein was successfully expressed in prokaryotic expression system. Besides, we have prepared the polyclonal antibody against recombinant prtein UL55, and characterized some properties of the duck enteritis virus UL55 protein for the first time. The research will be useful for further functional analysis of this gene.Duck enteritis virus (DEV), alternatively known as Duck plague virus (DPV), is a fatal pathogen of the family Anatidae of the order anseriformes[1], leading to an acute, febrile, contagious, and septic disease to waterfowls of all ages. The resulting disease designated as duck virus enteritis (DVE) has caused serious losses in commercial duck production in domestic and wild waterfowl since it was firstly discovered in Netherlands[2]. To our knowledge, DEV has been clustered to the subfamily of alphaherpesvirinae according to the report of the Eighth International Committee on Taxonomy of Viruses (ICTV)[3]. However, it has not been classified to any genus yet.The genome of DEV is composed of a linear, double stranded DNA. In recent ye
Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene
Ying Wu, Anchun Cheng, Mingshu Wang, Shunchuan Zhang, Dekang Zhu, Renyong Jia, Qihui Luo, Zhengli Chen, Xiaoyue Chen
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-266
Abstract: The newly identified duck enteritis virus UL55 gene was amplified and cloned into pMD18-T vector after digestion to generate a recombinant plasmid pMD18-T/UL55 for the establishment of qRT-PCR as standard DNA. The results of agarose gel electrophoresis and melting curve analysis demonstrated the primers we designed for qRT-PCR were specific and available. We used β-actin as a reference gene for normalization and established two standard curves based on pMD18-T/UL55 and pMD18-T/β-actin successfully. Based on that, the transcriptional analysis of DEV UL55 gene was performed, and the result suggested the expression of UL55 mRNA was at a low level from 0 to 8 h post-infection(p.i.), then accumulated quickly since 12 h p.i. and peaked at 36 h p.i., it can be detected till 60 h p.i.. Nucleic acid inhibition test was carried out for analyzing a temporal regulation condition of DEV UL55 gene, result revealed that it was sensitive to ganciclovir. Synthesis procedures of DEV UL55 gene can be inhibited by ganciclovir.The method we established in this paper can provide quantitative values reflecting the amounts of measured mRNA in samples. It's available for detection and quantification, also can be used in DEV diagnosis. The DEV UL55 gene was produced most abundantly during the late phase of replication in DEV-infected cells and the transcription of it depended on the synthesized DNA. DEV UL55 gene is a γ2 gene which occurs last and have a strict requirement for viral DNA synthesis.Duck viral enteritis(DVE) is one of the most widespread and destructive diseases of waterfowls in the family Anseriformes(ducks, geese, and swans)[1]. It is an acute, contagious and lethal disease which causes substantial mortalities in both farmed and wild waterfowl. Commercial waterfowl industry had been suffering considerable economic losses since it was discovered in Netherland[2]. Duck enteritis virus(DEV), alternatively known as duck plague virus(DPV), is the causative agent of DVE and has bee
Callus formation in relation to changes of the membrane Ca2+ in the graft unions ofGinkgo biloba L.
Xiong Yang,Zhengli Li
Chinese Science Bulletin , 1998, DOI: 10.1007/BF02883975
Abstract: The relationship between the callus formation and intracellular Ca2+ at the early stage of development of graft unions inGinkgo biloba L. was investigated. On the second day after grafting, part of the undamaged living cells at the graft interface of the stock and scion in the regions of the cortex and pith began to dedifferentiate or divide. 8 d after grafting, a great number of callus cells were produced from the cortex and phloem, while callus bridges linking the stock with scion formed between the callus cells of the graft partners from the cortex. A membrane Ca2+ probe, chlortetracycline (CTC), was used to locate the intracellular Ca2+ distribution and it was found that the cortical and pith parenchyma cells were the first to show remarkably increased CTC-Ca2+ fluorescence, suggesting that the cortical and other parenchyma cells play a leading role at the early stage of development of graft unions by earlier dedifferentiation and more rapid production of callus cells.
The Estimation of Trustworthy of Grid Services Based on Neural Network
Zhengli Zhai,Wei Zhang
Journal of Networks , 2010, DOI: 10.4304/jnw.5.10.1135-1142
Abstract: Though research on the Grid Services has progressed at a steady pace, its promise has yet to be realized. One major difficulty is that, by its very nature, the Grid Service is a large, uncensored system to which anyone may contribute. This raises the question of how much credence to give each source. The concept and definition of trustworthy of Grid Service is given. Estimating trustworthy of Grid Services with the method of Neural Network from the aspect of trustworthy history sequence is proposed. The principle of the method, applicable Neural Network structure, Neural Network constructing, input standardization, training sample constructing, and the procedure of estimating trustworthy of Grid Services with trained Neural Network are presented. Experiments confirm that the methods with Neural Network are feasible and effective to estimate trustworthy of Grid Service, and do not put unreasonable expectations on users. We hope that these methods will help to move the Grid Service closer to fulfilling its promise.
Innovation of the New Superior Quality Foxtail Millet [Setaria italica (L.) P.Beauv] Variety-Jigu32 with Characteristics of Stress Resistance, Stable and High Yield and Its Physiological Mechanism  [PDF]
Suying Li, Shengjun An, Zhengli Liu, Ruhong Cheng, Zhijun Wang
Agricultural Sciences (AS) , 2014, DOI: 10.4236/as.2014.54033
Abstract:

In main foxtail millet growing regions of China, natural disasters happen frequently, causing losses in production and finance. Therefore, it is urgently needed to breed new superior quality foxtail millet varieties with stress resistance, stable and high production, and, so as to stabilize millet production and promote millet industry development. Jigu32, a new foxtail millet variety with stable, high-yield and superior qualities, was developed using Target Character Gene Bank breeding method, and its physiological mechanism was studied as well. Results showed that the prominent characteristics of Jigu32 were as follows: 1) strong stress resistance and stable yielding; 2) high yielding; 3) rich calcium content and superior qualities; 4) excellent comprehensive characteristics. In 2010 National Foxtail Millet Regional Trials, the weather was tough. Severe drought occurred in some experimental stations while in some others, continuous rain, low temperature and little sunlight appeared. However, with the outstanding stress resistance, Jigu32 achieved the highest yields, and the yields were very stable under different conditions. Per unit yield of Jigu32 reached to5133.3 kg/hm

Characterization of duck enteritis virus UL53 gene and glycoprotein K
Shunchuan Zhang, Jun Xiang, Anchun Cheng, Mingshu Wang, Ying Wu, Xiaoyuan Yang, Dekang Zhu, Renyong Jia, Qihui Luo, Zhengli Chen, Xiaoyue Chen
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-235
Abstract: In our paper, the fluorescent quantitative real-time PCR(FQ-RT-PCR) assay and nucleic acid inhibition test were used to study the transcription characteristic of the DEV UL53 gene. Except detecting the mRNA of DEV UL53 gene, the product gK encoded by UL53 gene was detected through the expression kinetics of UL53 gene by the purified rabbit anti-UL53 protein polyclonal antibodies. Western-blotting and indirect immunofluorescence assays were used to detect gK. From the results of these experiments, the UL53 gene and gK were respectively identified as a late gene and a really late protein. On the other hand, the indirect immunofluorescence assay provided another information that the intracellular localization of DEV gK was mainly distributed in cytoplasm.By way of conclusions, we conceded that DEV UL53 gene is a really late gene, which is coincident with properties of UL53 homologs from other herpesvirus, such as ILTV(Infectious Laryngotracheitis virus) and HSV-1(Herpes simplex virus type 1). The properties of intracellular localization about gK protein provided a foundation for further functional analysis and further studies will be focused on constructing of the UL53 gene DEV mutant.Duck enteritis virus (DEV) is an alphaherpesvirinae that causes an acute, contagious and highly lethal disease in all ages of birds from the order Anseriformes (ducks, geese, and swans) [1-4]. DEV leads to heavy economic losses to the commercial duck industry due to its high mortality rate and decreased duck egg production [1].Whilst most of the previous research work had focused on the epidemiology and prevention of this disease [5,6]. With the development of protocols in molecular biology, nowadays more and more information about the genes of DEV was reported, such as UL5 [7], gC [8-10], UL24 [11-13], UL31 [14,15], UL35 [16,17], UL46 [18], UL38 [19], gE [20], UL51 [21], TK gene [22] and so on. While no information about DEV UL53 gene was known except our reported data [23,24], UL53 gene
Characterization and analysis of mitochondrial protein frataxin in Nosema bombycis
线粒体型蛋白frataxin在家蚕微孢子虫中的鉴定与分析

Junhua Hu,Guoqing Pan,Jingshan Xu,Kun Lu,Xiaoqun Dang,Zhengli Wu,Yanhong Li,Zeyang Zhou,
胡军华
,潘国庆,许金山,芦琨,党晓群,吴正理,李艳红,周泽扬

微生物学报 , 2008,
Abstract: 目的]Frataxin是铁硫簇相关蛋白,在线粒体代谢中起着重要作用.分析家蚕微孢子中该蛋白的结构特征,系统进化关系以及在孢子体内的转录翻译活性.方法]基于家蚕微孢子全基因组序列,同源序列搜索获得该基因序列.进行蛋白二级结构比较,近缘物种共线性特征分析及系统进化树构建.此外构建pGEX-4T-1-Nbfra原核重组表达载体,转化E coli BL21(DE3)进行目的蛋白表达纯化,以此为抗原免疫小鼠,制备抗体,并与家蚕微孢子总蛋白进行Western免疫杂交.结果]Nbfra蛋白缺乏进入线粒体的信号序列,功能区缺乏部分α-螺旋;frataxin基因在不同微孢子基因组中的分布具有共线性特征,表明其在基因组进化中非常保守.系统进化分析显示微孢子形成独立进化支,并且与高等真核生物近缘,说明微孢子在进化地位上比其它原虫更加高等,支持了微孢子虫是真菌的姊妹枝的进化地位假说.结论]免疫杂交结果表明Nbfra基因家蚕微孢子虫中能正常表达与翻译.本研究为微孢子的分类地位及线体假说提供了重要的补充依据.
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