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Search Results: 1 - 10 of 104194 matches for " Zhang Yukui "
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Advances of Chromatography in Analysis of Complex Samples
Zhang Yukui,
张玉奎

色谱 , 2004,
Abstract: The separation and identification of complex samples is an urgent task that analytical chemistry has to face. Chromatography, as an important analysis technique, has been widely applied to many fields, such as life science, environment, medicine, food and petrochemical engineering.Recently, with the progress of science and tech-
Correlation of Aquaporins and Transmembrane Solute Transporters Revealed by Genome-Wide Analysis in Developing Maize Leaf
Xun Yue,XiangYu Zhao,YuKui Fei,Xiansheng Zhang
Comparative and Functional Genomics , 2012, DOI: 10.1155/2012/546930
Abstract: Aquaporins are multifunctional membrane channels that facilitate the transmembrane transport of water and solutes. When transmembrane mineral nutrient transporters exhibit the same expression patterns as aquaporins under diverse temporal and physiological conditions, there is a greater probability that they interact. In this study, genome-wide temporal profiling of transcripts analysis and coexpression network-based approaches are used to examine the significant specificity correlation of aquaporins and transmembrane solute transporters in developing maize leaf. The results indicate that specific maize aquaporins are related to specific transmembrane solute transporters. The analysis demonstrates a systems-level correlation between aquaporins, nutrient transporters, and the homeostasis of mineral nutrients in developing maize leaf. Our results provide a resource for further studies into the physiological function of these aquaporins.
Correlation of Aquaporins and Transmembrane Solute Transporters Revealed by Genome-Wide Analysis in Developing Maize Leaf
Xun Yue,XiangYu Zhao,YuKui Fei,Xiansheng Zhang
International Journal of Genomics , 2012, DOI: 10.1155/2012/546930
Abstract: Aquaporins are multifunctional membrane channels that facilitate the transmembrane transport of water and solutes. When transmembrane mineral nutrient transporters exhibit the same expression patterns as aquaporins under diverse temporal and physiological conditions, there is a greater probability that they interact. In this study, genome-wide temporal profiling of transcripts analysis and coexpression network-based approaches are used to examine the significant specificity correlation of aquaporins and transmembrane solute transporters in developing maize leaf. The results indicate that specific maize aquaporins are related to specific transmembrane solute transporters. The analysis demonstrates a systems-level correlation between aquaporins, nutrient transporters, and the homeostasis of mineral nutrients in developing maize leaf. Our results provide a resource for further studies into the physiological function of these aquaporins. 1. Introduction Water can take different paths on its way through the leaf, in addition to radial water flux, water movement across leaf cell membranes is important for water homeostasis, increasing cell volume, maintaining turgor during expansion, regulating the opening and closure of stomata, and controlling leaf movement [1]. Water movement through cell membranes is facilitated by water channels called aquaporins. Plant aquaporins exhibit multiplicity and diversity, and fall into seven subfamilies loosely based on intracellular locations and sequence similarities: the plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), NOD26-like intrinsic proteins (NIPs), small, basic intrinsic proteins (SIPs), the GlpF-like intrinsic proteins (GIPs), hybrid intrinsic proteins (HIP), and the uncategorized X intrinsic proteins (XIP) [2]. Plant aquaporins are significant not only in plant-water relations, but also in physiological aspects such as nutrient transport and metal/metalloid toxicity [3, 4]. Flexas et al. provided evidence for the in vivo involvement of NtAQP1 in mesophyll CO2 conductance, suggesting a significant role for PIPs in CO2 diffusivity [5]. Ludewig and Dynowski have shown that AtTIP1;1 and AtTIP1;2 conduct H2O2 when heterologously expressed in yeast [6]. Azad et al. described TgTIP1;1- and TgTIP1;2-mediated H2O2 conductance by fluorescence assay in Tulipa gesneriana. Recent studies have investigated the selectivity mechanisms of aquaporins, nutrient transporters and homeostasis of mineral nutrients in most plant groups [7]. Hove and Bhave performed a comprehensive analysis of all plant
Impact of Mistiming on the Achievable Information Rate of Rake Receivers in DS-UWB Systems
Chunhua Geng,Yukui Pei,Jiaqi Zhang,Ning Ge
Mathematics , 2010,
Abstract: In this paper, we investigate the impact of mistiming on the performance of Rake receivers in direct-sequence ultra-wideband (DS-UWB) systems from the perspective of the achievable information rate. A generalized expression for the performance degradation due to mistiming is derived. Monte Carlo simulations based on this expression are then conducted, which demonstrate that the performance loss has little relationship with the target achievable information rate, but varies significantly with the system bandwidth and the multipath diversity order, which reflects design trade-offs among the system timing requirement, the bandwidth and the implementation complexity. In addition, the performance degradations of Rake receivers with different multipath component selection schemes and combining techniques are compared. Among these receivers, the widely used maximal ratio combining (MRC) selective-Rake (S-Rake) suffers the largest performance loss in the presence of mistiming.
Development of New Capillary Electrophoresis-Based Techniques for Protein Analysis
Hechun Liu,Chun Yang,Zhen Liang,Lihua Zhang,Yukui Zhang,
LIU Hechun
,YANG Chun,LIANG Zhen,ZHANG Lihua,ZHANG Yukui

色谱 , 2004,
Abstract: With the sequencing of human genome almost complete, human genome project enters the postgenome-sequencing era. Compared to genomics, the analysis of proteome is rather difficult since in human cells there are around 200 000 proteins, which are expressed at any time at different levels
A novel organic-inorganic hybrid monolith for trypsin immobilization
ShuaiBin Wu,JunFeng Ma,KaiGuang Yang,JinXiang Liu,Zhen Liang,LiHua Zhang,YuKui Zhang
Science China Life Sciences , 2011, DOI: 10.1007/s11427-010-4108-z
Abstract: In proteomics, attention has focused on various immobilized enzyme reactors (IMERs) for the realization of high throughput digestion. In this report, a novel organic-inorganic hybrid monolith based IMER was prepared in a 100 μm i.d. capillary with 3-glycidoxypropyltrimethoxysilane (GLYMO) as the monomer and tetraethoxysilane (TEOS) as the crosslinker. Trypsin immobilization was achieved via the reaction between vicinal diol groups, which were obtained from hydrolysis of epoxy groups, and the amino groups of trypsin. Bovine serum albumin was digested thoroughly by this IMER in 47 s. After micro-reverse phase liquid chromatography-tandem mass spectrometry (μRPLC-MS/MS) analysis and database searching, beyond 35% sequence coverage was obtained, and the result was comparable to that of 12 h in solution digestion. The present IMER has potential for high throughput digestion.
A novel organic-inorganic hybrid monolith for trypsin immobilization

WU ShuaiBin,MA JunFeng,YANG KaiGuang,LIU JinXiang,LIANG Zhen,ZHANG LiHua,ZHANG YuKui,

中国科学C辑(英文版) , 2011,
Abstract: In proteomics, attention has focused on various immobilized enzyme reactors (IMERs) for the realization of high throughput digestion. In this report, a novel organic-inorganic hybrid monolith based IMER was prepared in a 100 μm i.d. capillary with 3-glycidoxypropyltrimethoxysilane (GLYMO) as the monomer and tetraethoxysilane (TEOS) as the crosslinker. Trypsin immobilization was achieved via the reaction between vicinal diol groups, which were obtained from hydrolysis of epoxy groups, and the amino groups of trypsin. Bovine serum albumin was digested thoroughly by this IMER in 47 s. After micro-reverse phase liquid chromatography-tandem mass spectrometry (μRPLC-MS/MS) analysis and database searching, beyond 35% sequence coverage was obtained, and the result was comparable to that of 12 h in solution digestion. The present IMER has potential for high throughput digestion.
In Vivo Inhibitory Effect on the Biofilm Formation of Candida albicans by Liverwort Derived Riccardin D
Yan Li, Yukui Ma, Li Zhang, Feng Guo, Lei Ren, Rui Yang, Ying Li, Hongxiang Lou
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0035543
Abstract: Riccardin D, a macrocyclic bisbibenzyl isolated from Chinese liverwort Dumortiera hirsute, has been proved to have inhibitory effect on biofilms formation of Candida albicans in in vitro study. Our present study aims to investigate the in vivo effect and mechanisms of riccardin D against C. albicans biofilms when used alone or in combination with clinical using antifungal agent fluconazole. XTT reduction assay revealed riccardin D had both prophylactic and therapeutic effect against C. albicans biofilms formation in a dose-dependent manner when using a central venous catheter related infective animal model. Scanning electron microscope and laser confocal scanning microscope showed that the morphology of biofilms was altered remarkably after riccardin D treatment, especially hypha growth inhibition. To uncover the underlying molecular mechanisms, quantitative real-time RT-PCR was performed to observe the variation of related genes. The downregulation of hypha-specific genes such as ALS1, ALS3, ECE1, EFG1, HWP1 and CDC35 following riccardin D treatment suggested riccardin D inhibited the Ras-cAMP-Efg pathway to retard the hypha formation, then leading to the defect of biofilms maturation. Moreover, riccardin D displayed an increased antifungal activity when administered in combination with fluconazole. Our study provides a potential clinical application to eliminate the biofilms of relevant pathogens.
Separation and Recognition of Biomacromolecule by Molecular Imprinting Technique
分子印迹技术在生物大分子分离识别中的应用

ZHENG Chao,GAO Ruyu,ZHANG Yukui,
郑超
,高如瑜,张玉奎

色谱 , 2006,
Abstract: Molecular imprinting technique is a novel technique based on mimicking specific action of antibody-antigen.The emergence and the development of the technique are reviewed in this article.The focuses of this article include the introductions of the synthesis conditions,the comparisons of the various approaches on preparation methods as well as the recognition mechanisms of the biomacromolecule imprinted polymers.The primary synthetic methods include the embed technique,the surface imprinting procedure and the epitope approach.The epitope approach is based on using a short peptide as a template that represents only part of a larger peptide or protein,which in turn can be recognized by the synthesized polymer.This approach for the development of the biomacromolecule imprinted polymers selective to proteins is attractive from an economic viewpoint: a small peptide is usually less expensive,and the quantity necessary for the polymer preparation is more readily available than that of the corresponding protein.In the end,the limitations and the prospective applications of this biomacromolecular imprinted technique are also discussed.
Foreword
引言

ZHANG Yukui,GUAN Yafeng,
张玉奎
,关亚风

色谱 , 2007,
Abstract: 样品预处理方法的重复性对整个定量分析数据的可靠性、重复性具有重要的甚至是决定性的影响,特别是在采用色谱和色谱-质谱、色谱-光谱等联用技术进行分析时更是如此。由于这些分析技术的重复误差都小于5%甚至更低,而样品前处理的重复误差往往在5%~15%之间,因此成为整个分析精度的控制因素。如何提高样品前处理技术的重复精度,使之接近甚至达到分析仪器的重复精度,是样品前处理技术研究的挑战之一。样品预处理方法的富集倍数决定了分析检测的灵敏度或检测下限(LOD)。由于发达国家对食品、药物、日用品和环境中有毒有害物质检测下限的标准…
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