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Search Results: 1 - 10 of 119650 matches for " Zeng-Rong Zhang "
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Identification and Association of the Single Nucleotide Polymorphisms in Calpastatin (CAST) Gene with Carcass Traits in Chicken
Yao-Dong Hu,Zeng-Rong Zhang,Qing Zhu
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2011.2968.2974
Abstract: Calpastatin (CAST) is a naturally occurring protein that inhibits normal tenderization of meat as it ages postmortem. The aim of this study was to investigate the effect of CAST gene polymorphisms on chicken carcass traits. The researchers screened CAST Single Nucleotide Polymorphisms (SNP) in 359 meat type quality chickens from 5 commercial pure lines (S01, S02, S03, S05 and D99; developed from Chinese local breeds), 3 crossbreeds (S01xS05, S01xS10 and S01xD99) and 4 native breeds from Guangdong (Fengkai Xinghua chicken, Huiyang Huxu chicken, Qingyuan Ma chicken) and Guangxi province (Xiayan chicken) in China. Three SNPs (36127T>C, 37752A>T and 37868G>A) were detected by Single Strand Conformation Polymorphism (SSCP) method and DNA sequencing. The linkage disequilibrium analyze found that only 37868G>A SNP in Hardy-Weinberg equilibrium yet one SNP can not compose haplotype, therefore these three SNPs should be analyzed separately rather than as haplotypes. Association analysis showed that the 37752A>T genotypes were significantly associated with Body Weight (BW), Carcass Weight (CW), Breast Muscle Weight (BMW) and Leg Muscle Weight (LMW). The results suggest that CAST SNP is significantly associated with carcass trait in the twelve studied populations and could be useful in selection for changing meat quality in chicken. Further investigations on more chicken populations with larger sample size are needed to confirm this conclusion.
An Approach to Analyse Phase Synchronization in Oscillator Networks with Weak Coupling
ZHANG Jian-Bao,LIU Zeng-Rong,LI Ying,

中国物理快报 , 2007,
Abstract: We study phase synchronization in oscillator networks through phase reduced method. The dynamics of networks is reduced to phase equations by this method. Analysing the phase equations through the master stability function method, one obtains that the oscillators with identical frequency can be in-phase synchronized by weak balanced coupling. Similarly, the problem of frequency synchronization of oscillators with different frequencies is transformed to the existence of a locally asymptotically stable equilibrium of the phase error system.
Synchronization between Different Networks
LI Ying,LIU Zeng-Rong,ZHANG Jian-Bao,

中国物理快报 , 2008,
Abstract: Synchronization between two networks with different topology structures and different dynamical behaviours is studied. These two different networks are driving and responding networks, respectively. Under the preconditions that the driving network gets synchronization, we give the conditions for the responding network to be synchronized to the same dynamics as the driving network with the help of the open-plus-closed-loop method. Then a example is given to verify the validity of the theoretical results.
Robust and Adaptive MicroRNA-Mediated Incoherent Feedforward Motifs

XU Feng-Dan,LIU Zeng-Rong,ZHANG Zhi-Yong,SHEN Jian-Wei,

中国物理快报 , 2009,
Dynamics of network motifs in genetic regulatory networks
Li Ying,Liu Zeng-Rong,Zhang Jian-Bao,

中国物理 B , 2007,
Abstract: Network motifs hold a very important status in genetic regulatory networks. This paper aims to analyse the dynamical property of the network motifs in genetic regulatory networks. The main result we obtained is that the dynamical property of a single motif is very simple with only an asymptotically stable equilibrium point, but the combination of several motifs can make more complicated dynamical properties emerge such as limit cycles. The above-mentioned result shows that network motif is a stable substructure in genetic regulatory networks while their combinations make the genetic regulatory network more complicated.
Tissue-Specific Expression of the Chicken Calpain2 Gene
Zeng-Rong Zhang,Xiao-Song Jiang,Hua-Rui Du,Xiao-Cheng Li,Qing Zhu,Yi-Ping Liu
Molecular Biology International , 2010, DOI: 10.4061/2010/373241
Abstract: We quantified chicken calpain 2 (CAPN2) expression in two Chinese chicken breeds (mountainous black-bone chicken breed [MB] and a commercial meat type chicken breed [S01]) to discern the tissue and ontogenic expression pattern and its effect on muscle metabolism. Real-time quantitative PCR assay was developed for accurate measurement of the CAPN2 mRNA expression in various tissues from chickens of different ages (0, 2, 4, 6, 8, 10, and 12 weeks). Results showed that the breast muscle and leg muscle tissues had the highest expression of CAPN2 compared to the other tissues from the same individual ( ). Overall, the CAPN2 mRNA level exhibited a “rise” developmental change in all tissues. The S01 chicken had a higher expression of the CAPN2 mRNA in all tissues than the MB chicken. Our results suggest that chicken CAPN2 expression may be related to chicken breeds and tissues. 1. Introduction Nowadays a highlighted issue for an increasing number of consumers is the problem of meat tenderness, as the result of its physicochemical and biochemical mechanisms acting mainly on myofibrillar structures postmortem. Calpains play an important role in degradation of muscle protein. Because calpastatin inhibits both calpain1 and calpain2, it remains unclear whether one or both calpains are active in postmortem muscle. Most of the studies that have used appropriate methodology [1] have found the same effect of postmortem storage on the activity of the components of the calpain system [2–4]. This conclusion seems to be based on the finding that calpain2 is not autolyzed during postmortem storage [5, 6]. But some studies also have now suggested that both unautolyzed calpain1 and calpain2 are proteolytically active [7, 8]. The lack of expression data of the calpain2 gene in chicken makes it difficult to verify the role of calpain2 in control of meat quality and carcass traits. In this study, we aimed to (1) develop a convenient approach to quantify the abundance of the calpain2 transcripts in chicken tissues and (2) determine tissue distribution and ontogenic expression of this gene, particularly in muscle tissues. 2. Materials and Methods 2.1. Animals Thirty-six chickens at different ages from two breeds/populations, Mountainous black-bone chicken (MB) and a commercial chicken (S01) from Sichuan province, were used in this study. All populations were raised under the same condition and were randomly selected. Tissue samples (including heart, liver, breast muscle, leg muscle, brain, and abdominal fat) from MB chickens were collected at 0, 2, 4, 6, 8, and 10?w, respectively;
Identification and association of the single nucleotide polymorphisms in calpain3 (CAPN3) gene with carcass traits in chickens
Zeng-Rong Zhang, Yi-Ping Liu, Yong-Gang Yao, Xiao-Song Jiang, Hua-Rui Du, Qing Zhu
BMC Genetics , 2009, DOI: 10.1186/1471-2156-10-10
Abstract: Two SNPs (11818T>A and 12814T>G) were detected by single strand conformation polymorphism (SSCP) method and were verified by DNA sequencing. Association analysis showed that the 12814T>G genotypes were significantly associated with body weight (BW), carcass weight (CW), breast muscle weight (BMW), and leg muscle weight (LMW). Haplotypes constructed on the two SNPs (H1, TG; H2, TT; H3, AG; and H4, AT) were associated with BW, CW (P < 0.05), eviscerated percentage (EP), semi-eviscerated percentage (SEP), breast muscle percentage (BMP), and leg muscle percentage (LMP) (P < 0.01). Diplotype H1H2 was dominant for BW, CW, and LMP, and H2H2 was dominant for EP, SEP, and BMP.We speculated that the CAPN3 gene was a major gene affecting chicken muscle growth and carcass traits or it was linked with the major gene(s). Diplotypes H1H2 and H2H2 might be advantageous for carcass traits.The accumulation of muscle mass in the body is determined by the relative rates of muscle protein synthesis and degradation, namely, this process is closely related to the muscle protein turnover. Studies on the rate of muscle growth in layer and broiler chickens have shown that the rate of muscle growth are mainly determined by changes in the rate of muscle protein degradation, with little or no change in the rate of muscle protein synthesis [1].Calpains, i.e. intracellular Ca2+-dependent cysteine proteases (EC, have long been demonstrated to be involved in muscle growth and development and are proenzymes that are regulated by Ca2+ binding and autoproteolytic modification [2]. The calpains were discovered because the Z-disks in muscle strips incubated in a Ca2+-containing solution disappeared but with no other ultrastructurally detectable change [3]. Four calpain genes (CAPN1, CAPN2, CAPN3, and CAPN1.5) are expressed ubiquitously in chicken [4]. Study have further showed that there was less calpain activity, but more calpastatin, in broiler breast muscle [5]. Among the calpain family me
Study on correlation between single nucleotide polymorphism of CAPN1 gene and muscle tenderness and carcass traits in chicken

ZHANG Zeng-Rong,ZHU Qing,JIANG Xiao-Song,DU Hua-Rui,

遗传 , 2007,
Abstract: In order to investigate the effect of CAPN1 on broiler tenderness traits,its CDS was analyzed with PCR-SSCP and DNA sequencing,and the genotype analyses covered individuals from five purelines and three crossbreds. Primers for exon5 and exon6 of CAPN1 were designed from database of chicken genomic sequence. The frequency of allele and PIC were calculated. A C/T mutation at base position 2546(site A) and a G/A mutation at base position 3535(site B) were found among individuals in each line. The least square analysis showed that three genotypes of birds had significant correlation with fiber density and partial carcass traits in site A and B. It was concluded from the results that CAPN1 gene may be the major gene affecting the tenderness traitsof chicken or it links with the major gene,and the the polymorphic site may be used as molecular markers for meat tenderness and chicken quality.
On two-stage adaptive cluster sampling to assess pest density.

ZHANG Nan-song,HU Bing-min,ZHU Zeng-rong,

浙江大学学报(农业与生命科学版) , 2000,
Abstract: 在农作物害虫管理中人们需要得到准确的田间数据。本文提出了一种简便的抽样方法--二阶适应性整群抽样,给出了害虫密度的无偏估计,并举例说明。
Synchronization between different motifs

Li Ying,Liu Zeng-Rong,

中国物理 B , 2010,
Abstract: In this paper, we study the synchronization between different motifs. First, the synchronization between two networks with different topology structures and different dynamical behaviours is studied. With the open-plus-closed-loop(OPCL) method, conditions for two different networks to realize synchronization are given. Then based on the theoretical results achieved, the synchronization between different motifs is studied, which verifies the effectiveness and feasibility of the synchronization scheme.
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