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Search Results: 1 - 10 of 98899 matches for " ZHOU Yu-Bao "
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Cloning, expression, and analysis of the group 2 allergen from Dermatophagoides farinae from China
Yu-bao, Cui;Zhou, Ying;Weihong, Shi;Guifang, Ma;Yang, Li;Yungang, Wang;
Anais da Academia Brasileira de Ciências , 2010, DOI: 10.1590/S0001-37652010000400017
Abstract: to obtain the recombinant group 2 allergen product of dermatophagoides farinae (der f 2), the der f 2 gene was synthesized by rt-pcr. the full-length cdna comprised 441 nucleotides and was 99.3% identical to the reference sequence (genbank ab195580). the cdna was bound to vector pet28a to construct plasmid pet28a(+)-der f 2, which was transformed into e. coli bl21 and induced by iptg. sds-page showed a specific band of about 14kda in the hole cell lysate. s estiated by chroatography, about 3.86 g of the recobinant product as obtained, which conjugated with serum ige from asthmatic children. the protein had a signal peptide of 17 amino acids. its secondary structure comprised an alpha helix (19.86%), an extended strand (30.82%), and a random coil (49.32%). the subcellular localization of this allergen was predicted to be at mitochondria. furthermore, its function was shown to be associated with an md-2-related lipid-recognition (ml) domain. the results of this study provide a solid foundation for large-scale production of the allergen for clinical diagnosis and treatent of allergic disorders.
Study on Preparation of n-HA and Silk Fibroin Bio-mineral Material
无机材料学报 , 2009, DOI: 10.3724/sp.j.1077.2009.00264
Abstract: A new hydroxyapatite (n-HA)a2Silk fibroin (SF) bio-mineral material was prepared by using a biomimetic method, with Ca(NO3)2 and Na3PO4 used as the starting reagent to synthesize the inorganic phase. In details, SF was dissolved in Ca(NO3)2 solution without desalting procedure, the compound solution was directly dropped into Na3PO4 solution. In this way, HA was synthesized at 37 y controlling Ca/P ratio, meanwhile the minerialized HA interacted with SF to form biomineral material. The morphology and structure of this n-HA-SF biomineral material were characterized by XRD, FTIR, XPS and SEM. The results show that there are strong chemical interaction between n-HA and SF. Moreover, in the biomineral material, the inorganic phase is calciuma2deficient hydroxyapatite, containing a small amount of carbonated HA with an average crystal size of 37.6nm. In addition, The particle size of biomineral materials range from 50nm to 200nm.It can be deduced that silk fibroin chain can regulate the growth and morphology of HA crystals. The compressive strength of the biomineral material is 32.21MPa£ which is a good potential material for bone tissue engineering and unloadeda2bearing bone defects repairing.
Study on Preparation of n-HA and Silk Fibroin Bio-mineral Material

王 江

无机材料学报 , 2009,
Abstract: 以Ca(NO3)2与Na3PO4为无机相的前驱体,将丝素蛋白直接溶于Ca(NO3)2溶液中,不经过脱盐处理,直接滴入Na3PO4溶液中反应,在37℃下丝素蛋白和羟基磷灰石晶体之间相互作用,仿生合成了纳米羟基磷灰石(n-HA)-丝素蛋白(SF)生物矿化材料.用FTIR、XRD、XPS和SEM进行表征.结果表明,羟基磷灰石和丝素蛋白两相间具有较强的化学键合,矿化材料中无机相包含少量碳酸根,为缺钙类骨羟基磷灰石并且呈现一定的长轴取向性,说明丝素蛋白大分子对羟基磷灰石晶体的成核和生长起着模板和调控作用.矿化物颗粒尺寸在50~200nm之间,其抗压强度为32.21MPa,可作为非承重部位骨组织缺损修复材料.
Genetic heterogeneity of Metarhizium anisopliae populations from the soils collected from Anhui Province


菌物学报 , 2013,
Abstract: 金龟子绿僵菌是昆虫种群自然控制和害虫生物防治中重要的虫生真菌,已被作为化学农药的替代品广泛应用于农林害虫的防治。其寄主范围和地理分布极广,因此种群结构也十分复杂。为明确安徽省土栖金龟子绿僵菌的种群遗传结构,采用ISSR分子标记技术对采集自安徽省不同地区土壤中的116株金龟子绿僵菌进行遗传异质性分析。分子数据显示,筛选出的8个引物共获得79个位点,其中多态位点比率为100%。不同地区种群的Nei’s基因多样性(H)为0.2796,Shannon信息指数(I)为0.4425,种群间存在一定程度的基因流(Nm=4.4282)和遗传分化(Gst=0.1015),种群内的基因多样度占总居群的89.85%,种群间占10.15%,表明安徽土栖金龟子绿僵菌的遗传变异主要来源于种群内,且种群内表现出较大的遗传变异。并采用UPGMA对所有供试材料进行聚类分析,得到7大类种群。结果表明,安徽土栖金龟子绿僵菌之间的亲缘关系与地理来源不存在相关性。
Setting Mechanism of Nano-hydroxyapatite/Chitosan Bone Cement

ZHANG Li,LI Yu-Bao,ZHOU Gang,L Guo-Yu,ZUO Yi,

无机材料学报 , 2006,
Abstract: A n-HA/chitosan composite cement containing ZnO as the coagulant was prepared, and its physic-chemical properties and the setting mechanism were also investigated. The results showed that both the compressive strength and the corresponding setting time of the cement could meet with the demand in clinic. A chelate having the tight network structure was formed due to the reaction of Zn2+ and Ca2+ ions with amino group of chitosan, which produced a contractive force. The force extruded H2O molecules out of the cement body and made the cement set fast, and with higher compressive strength.
Effects of endophyte infection on ecophysiological response of perennial ryegrass under phosphorus deficiency

REN An-Zhi,GAO Yu-Bao,ZHOU Fang,CHEN Lei,

生态学报 , 2007,
Abstract: 选取感染和未感染的黑麦草为材料,在田间盆栽条件下研究内生真菌感染对宿主植物抵抗磷胁迫方面的贡献。结果表明,土壤中缺磷或内生真菌感染对黑麦草地上部生长的影响不显著,但内生真菌感染对植株地下部生长和生理指标有明显影响。缺磷条件下,内生真菌感染有助于黑麦草地下部分的生长,表现在根系总长度更长,生物量更大;同时根中酚类物质和有机酸的含量也显著高于未感染植株,但因酚类物质和有机酸总量增加的同时并未伴随着二者浓度的增加,由此推测,内生真菌在改变宿主黑麦草根系代谢活动方面的贡献有限。此外,内生真菌感染显著提高了宿主植物的磷利用效率,这可能和缺磷条件下内生真菌感染植株具有更高的酸性磷酸酶活性有关。

Zhou Wei-y,Li Yu-bao,Liu Zu-qin,Tang Dong-sheng,Zou Xiao-ping,Wang Gang,

中国物理 B , 2001,
Abstract: Conditions for a self-organized formation of ordered hexagonal structure in anodic alumina were investigated, using oxalic or sulphuric acid as an electrolyte. Highly-ordered nanopore arrays with pore densities of 9×109-6.5×1010cm-2 and high aspect ratios over 3000 were fabricated by a two-step anodization process. The array exhibits characteristics analogous to a two-dimensional polycrystalline structure of a few micrometres in size. The interpore distance can be controlled by changing the electrolyte and/or the applied voltage. The formation mechanism of ordered arrays is consistent with a previously proposed mechanical stress model, i.e., the repulsive forces between neighbouring pores at the metal/oxide interface promote the formation of hexagonally ordered pores during the oxidation process.

Liu Zu-qin,Sun Lian-feng,Tang Dong-sheng,Zhou Wei-y,Li Yu-bao,Zou Xiao-ping,Wang Gang,

中国物理 B , 2000,
Abstract: Amorphous silicon (a-Si) nanowires have been prepared on SiO2/Si substrates by AuPd nanoparticles / silane reaction method. Field-emission scanning electron microscopy and transmission electron microscopy were used to characterize the samples. The typical a-Si nanowires we obtained are of a uniform diameter about 20 nm and length up to several micrometers. The growth mechanism of the nanowires seems to be the vapor-liquid-solid mechanism. The catalytic particle size effect on the formation of the nanowires and the cause of forming amorphous state Si nanowires are discussed.
Review on Global Ecological Degradation in the Last 30 Years of 20th Century
20世纪末30 a里全球生态退化状况

HE Xing-dong,CONG Pei-fang,DONG Zhi-bao,GAO Yu-bao,ZHOU Qi-xing,

中国沙漠 , 2007,
Abstract: Ecological degradation mainly includes degradation of terrestrial and aquatic ecosystems. Terrestrial ecosystem includes farmland, forest and rangeland, and aquatic ecosystem includes inshore, rivers and lakes. The ecological outcomes of the degradation mainly present on productivity reducing, biodiversity losing and ecological service functions declining, and directly represent on the land degradation, forest reduction, species endangering and extinction as well as fresh water shortage. By means of the reports on websites of Unite Station, some issues to ecological degradation were reviewed to promote ecosystem restoration in this paper.
Antibacterial Effect and the Mechanism of Cu2+, Zn2+ Bearing Nano-hydroxyapatite

LI Ji-Dong,LI Yu-Bao,WANG Xue-Jiang,YANG Wei-Hu,ZHOU Gang,

无机材料学报 , 2006,
Abstract: Nano-hydroxyapatite (n-HA) slurry was synthesized at normal pressure, and Cu-bearing nano-hydroxyapatite (Cu-HA), Zn-bearing nano-hydroxyapatite (Zn-HA) and (Cu, Zn)-bearing nano-hydroxyapatite (Cu-Zn-HA) were prepared by the ion exchange method in water medium. The properties of antibacterial materials were characterized by AAS, XRD and TEM. The results of XRD analysis indicate that Cu2+ and Zn2+ can occupy Ca2+ site and also enter the lattice of hydroxyapatite. The results of antibacterial experiments show that Cu-HA and Cu-Zn-HA have better antibacterial ability on E. coli and S. aureus, while the antibacterial effect of Zn-HA is not significant. From the analysis of the antibacterial results, it can be concluded that two mechanisms play the important role in the antibacterial ability, one is electrostatic attraction which making large amount of bacteria adhere on the n-HA surface, the other is the release of antibacterial metal ions slowly into medium which can inhibit and kill bacteria.
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