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Search Results: 1 - 10 of 34491 matches for " ZHOU Cuifen "
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One-Step Microemulsion-Mediated Hydrothermal Synthesis of Nanocrystalline TiO2  [PDF]
Xuyao Xu, Xiaosong Zhou, Lin Ma, Miaoyan Mo, Cuifen Ren, Rongkai Pan
World Journal of Nano Science and Engineering (WJNSE) , 2014, DOI: 10.4236/wjnse.2014.41005
Abstract:
Nanocrystalline TiO2 powders with high photocatalytic activity were prepared by one-step microemulsion-mediated hydrothermal method using tetrabutylorthotitanate (TiO(C4H9)4, TBOT) as precursor. The as-prepared TiO2 powders were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and the Brunauer-Emmett-Teller (BET) specific surface area measurements. The effects of the oil/water ratio and hydrothermal temperature on the microstructures and photocatalytic activity of the TiO2 powders were investigated. The results suggest that increasing the oil/water emulsion ratio significantly decreased the particle size of the as-prepared TiO2 powders and improved the photocatalytic activity. With hydrothermal temperature increasing, the average crystallite size increased and the photocatalytic activities of TiO2 powders decreased.
Establishment of Smad2 conditional gene targeting mice based on the Cre-LoxP system
Jiang Zhou,Xuan Cheng,Yanxun Sun,Peitang Huang,Cuifen Huang,Xiao Yang
Science China Life Sciences , 2002, DOI: 10.1360/02yc9015
Abstract: Smads is a new gene family in transforming growth factor-β (TGF- β) signaling pathway. Smad2 mutated in multiple human tumors and may be a candidate tumor suppressor gene. Targeted disruption of murine Smad2 gene resulted in embryonic lethality at E6.5. To study the function of Smad2 in vertebrate organgenesis and tumorigenesis, we constructed the Smad2 conditional targeting vector in which two LoxP sequences were placed to flank the sequences encoding the C terminal functional domain of Smad2. The validity of the LoxP sites in the targeting construct was tested in E. coli that express the Cre recombinase constitutively. The vector was electroporated into ES cells and 3 targeted ES cell clones were obtained by Southern blot screening. Targeted ES cells were introduced into C57BL/6J blastocysts by microinjection to generate germ-line chimeras. Genotyping analysis showed that 2 progeny among these chimeras carried the Smad2 conditional targeted allele. The establishment of Smad2 conditional gene targeting mouse has laid a solid foundation for producing the tissue specific Smad2 gene knockout mice.
Preliminary study on discovery of a novel molecule that interacts with CPP32 using two-hybrid system
Ye Qinong,Yao Xiao,Wang Hengliang,Su Guofu,Huang Cuifen,Zhou Tingchong
Chinese Science Bulletin , 1998, DOI: 10.1007/BF02884540
Abstract: Of ICE protease family. CPP32 (apopain or Yama) plays a central mle in different apoptotic pathways. To study the moleculcs regulating CPP32, yenst twehyhrid system was used to identify proteins (peptides) that interact with CPP32. First, the CPP32 gene was cloned into plasmid vector pGBT9. The resulting recombinant plasmid was designated as pCBT9/CPP. The pG-BT9 /CPP plasmid was transformed into the yeast stram HF7C, then the leukemia library was introduced. The transformation mixture was plated on medium lacking Trp, Leu and His in the initial screen. Colonies growing on the selection medium were further aswyed for Sgalactosidase activity. Within 42 Trp+ Leu+ His+ colonies only 5 turned blue in the presence of X-Gal. hid DNA from 5 positive yeast mlonies was prepared respectively and used to transform HB101 by electroporation. The transformation mixture was plated on medium lacking Leu to be selected for the library plasmid. Finally, only one library plmmid, designated as pY1. was determined to be truly pchsitive by retransformation of pGBT9/CPP and the library plasmid into HF7C. The inserted cDNA of pY1 encodes a peptide of 15 amino acids, suggesting that it may be the domain interacting with CPP32.
A novel aberrant splicing of Gsα transcript in human leukemia cell lines
Qinong Ye,Xiao Yao,Hengliang Wang,Shu Zhang,Huaitian Liu,Guofu Su,Cuifen Huang,Tingchong Zhou
Chinese Science Bulletin , 1999, DOI: 10.1007/BF02885487
Abstract: The a subunit of the stimulatory G protein, Gsα, is involved in the stimulation of adenylate cyclase pathway of signal transduction. The various Gsα, mRNAs are generated either by alternative splicing or by using alternative promoter. A novel aberrant splicing of Gsα transcript in human leukemia cell lines is reported. The entire coding region of Gsα gene obtained, referred to as GsαLeu, was amplified by PCR with cDNA from Jurkat human leukemia cDNA library or the reverse-transcribed first strand cDNA from human leukemia cell line HL-60 as a template. The result of DNA sequencing indicated that, compared with originally reported Gsα, GsαLeu has two in-frame deletions, one in amino acid residues 23–249 and the other in amino acid residues 254–260, suggesting that GsαLeu, may play an important role in leukemia. The complete coding region of GsαLeu was inserted downstream of the thrombin site of pGEX-2T fusion protein expression vector and expressed in the form of GST/GsαLeu, at high level, which was then purified to be electrophoretically pure by one-step affinity chromatography. The expression of GsαLeu lays foundation for the study of its function.
Preliminary study on discovery of a novel molecule that interacts with CPP32 using two-hybrid system

Ye Qinong,Yao Xiao,Wang Hengliang,Su Guofu,Huang Cuifen,Zhou Tingchong,

科学通报(英文版) , 1998,
Abstract: Of ICE protease family. CPP32 (apopain or Yama) plays a central mle in different apoptotic pathways. To study the moleculcs regulating CPP32, yenst twehyhrid system was used to identify proteins (peptides) that interact with CPP32. First, the CPP32 gene was cloned into plasmid vector pGBT9. The resulting recombinant plasmid was designated as pCBT9/CPP. The pG-BT9 /CPP plasmid was transformed into the yeast stram HF7C, then the leukemia library was introduced. The transformation mixture was plated on medium lacking Trp, Leu and His in the initial screen. Colonies growing on the selection medium were further aswyed for Sgalactosidase activity. Within 42 Trp+ Leu+ His+ colonies only 5 turned blue in the presence of X-Gal. hid DNA from 5 positive yeast mlonies was prepared respectively and used to transform HB101 by electroporation. The transformation mixture was plated on medium lacking Leu to be selected for the library plasmid. Finally, only one library plmmid, designated as pY1. was determined to be truly pchsitive by retransformation of pGBT9/CPP and the library plasmid into HF7C. The inserted cDNA of pY1 encodes a peptide of 15 amino acids, suggesting that it may be the domain interacting with CPP32.
Establishment of murine Smad5 double knockout ES cells and the studies on their properties
Xiao Yang,Yanxun Sun,Jiang Zhou,Peitang Huang,Cuifen Huang,Xiaoling Xu,Cuiling Li,Jessica Gotay,Lin Chen,Chuxia Deng
Science China Life Sciences , 2001, DOI: 10.1007/BF02879316
Abstract: Smad5 is an intracellular transducer of TGF-β signals. Targeted disruption of murineSmad5 gene resulted in embryonic lethal. To study the function ofSmad5 in organgenesis, we generatedSmad5 double knockout ES cells by homologous recombination. We deleted theneo gene of theSmad5 targeted ES cells using Cre-LoxP system.Smad5 double knockout ES cells were obtained by transfecting the targeted ES cells using the same targeting construct. The results of chimeric study showed thatSmad5 might play an important role during the development of heart and neural tube.Smad5 double knockout ES cells formed teratoma when injected subcutaneously into nude mice. They differentiated into several types of cells, including neural cells, muscle cells, chondrocytes, endothelial cells and glandaceous cells.Smad5 double knockout ES cells are useful for studying the function ofSmad5 mediated TGF-β during the organgenesis and thein vitro differentiation of ES cells.
Smart Container Security: the E-seal with RFID Technology
Jin Zhang,Cuifen Zhang
Modern Applied Science , 2009, DOI: 10.5539/mas.v1n3p16
Abstract: In order to protect cargo from damage, theft, and terrorist threats, business and government turn to wireless sensors and RFID tags, and tradition container is replaced by smart container. In this paper, the basic technical features of RFID systems are described and linked to the practical applications. This paper will also determine how the technologies perform in the real-world operational environments and evaluate the various trade-offs that exist with E-seal design.
Heterologous Genes Expression on Escherichia coli Chromosome lac Operon Using Red Recombination
利用Red重组工程技术解决外源基因在大肠杆菌染色体lac操纵子中的表达

Shanhu Li,Qingguo Shi,Cuifen Huang,Jianguang Zhou,
李山虎
,施庆国,黄翠芬,周建光

微生物学报 , 2008,
Abstract: To achieve efficient and stable expression of heterologous exogenetic protein or antigen in E. coli chromosome, the luciferase report gene was knocked in lacZ site of chromosome lac operon by using Red recombination system and selection-counterselection kan/sacB technology. The quantitative analysis of exogenous gene expression indicated that the target gene could be efficiently expressed at lacZ site of lac operon. The results confirmed the efficient screening and stable expression of heterologous protein or antigen on chromosome by using the recombinant engineering technique. This study demonstrated that the chromosome could be used as a vector for heterologous protein or antigen and the stable expression of exogenous gene on E. coli chromosome had no side effect on the bacterial growth and propagation.
Heterologous Genes Expression on Escherichia coli Chromosome lac Operon Using Red Recombination
利用Red重组工程技术解决外源基因在大肠杆菌染色体lac操纵子中的表达

Shanhu Li,Qingguo Shi,Cuifen Huang,Jianguang Zhou,
李山虎
,施庆国,黄翠芬,周建光

生物工程学报 , 2008,
Abstract: To achieve efficient and stable expression of heterologous exogenetic protein or antigen in E. coli chromosome, the luciferase report gene was knocked in lacZ site of chromosome lac operon by using Red recombination system and selection-counterselection kan/sacB technology. The quantitative analysis of exogenous gene expression indicated that the target gene could be efficiently expressed at lacZ site of lac operon. The results confirmed the efficient screening and stable expression of heterologous protein or antigen on chromosome by using the recombinant engineering technique. This study demonstrated that the chromosome could be used as a vector for heterologous protein or antigen and the stable expression of exogenous gene on E. coli chromosome had no side effect on the bacterial growth and propagation.
Establishment of Smad2 conditional gene targeting mice based on the Cre-LoxP system
ZHOU Jiang,CHENG Xuan,SUN Yanxun,HUANG Peitang,HUANG Cuifen,YANG Xiao,
周江
,程萱,孙彦洵,黄培堂,黄翠芬,杨晓

中国科学C辑(英文版) , 2002,
Abstract: Smads is a new gene family in transforming growth factor-@ (TGF- @) signaling pathway. Smad2 mutated in multiple human tumors and may be a candidate tumor suppressor gene. Targeted disruption of murine Smad2 gene resulted in embryonic lethality at E6.5. To study the function of Smad2 in vertebrate organgenesis and tumorigenesis, we constructed the Smad2 conditional targeting vector in which two LoxP sequences were placed to flank the sequences encoding the C terminal functional domain of Smad2. The validity of the LoxP sites in the targeting construct was tested in E. coli that express the Cre recombinase constitutively. The vector was electropo-rated into ES cells and 3 targeted ES cell clones were obtained by Southern blot screening. Targeted ES cells were introduced into C57BL/6J blastocysts by microinjection to generate germ-line chimeras. Genotyping analysis showed that 2 progeny among these chimeras carried the Smad2 conditional targeted allele. The establishment of Smad2 conditional gene targeting mouse has laid a solid foundation for producing the tissue specific Smad2 gene knockout mice.
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