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Search Results: 1 - 10 of 31010 matches for " Z Hosseininejad "
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Whatman Paper (FTA Cards) for Storing and Transferring Leishmania DNA for PCR Examination
A Fata,A Khamesipour,M Mohajery,Z Hosseininejad
Iranian Journal of Parasitology , 2009,
Abstract: "nBackground: Diagnosis of cutaneous leishmaniasis (CL) is often made based on clinical manifesta-tion. Correct diagnosis and identification of the parasite are crucial for choosing the effective treat-ment and for epidemiological studies. On the other hand, determination of Leishmania species is nec-essary for designing appropriate control programs. Diagnosis by PCR is becoming a 'gold standard'. For PCR preparation, storage and shipments of specimens are necessary. In this study, Whatman filter paper (FTA Card) was used to store and transfer samples for Leishmania identification using PCR. "nMethods: Among the patients who had CL lesion and referred to Parasitology Laboratory of Emam Reza Hospital, Mashhad, Iran, 44 consented cases with positive results in their direct smear were se-lected. An informed consent form and a questionnaire were completed and three different types of samples (direct smear, NNN culture, and spot on FTA card) were collected. DNA extraction and PCR were carried out on three different samples from each patient. "nResults: PCR results using Whatman paper samples revealed a significant difference (P<0.0001) compared to the culture method but no significant difference was seen between PCR results using samples stored on Whatman paper and direct smears. "nConclusion: The use of FTA cards is simple, rapid, and cost-effective, and can be readily employed for large-scale population screening, especially for regions where the specimens are to be transported from distant places to the laboratory.
Serological Evaluation of Experimental Toxoplasma gondii Infection in Cats by Using Immunoblotting Based on an Affinity Purified Surface Antigen
Hosseininejad, M.
Archives of Razi Institute , 2012,
Abstract: Toxoplasma gondii is an apicomplexan parasite that infects human and almost all warm-blooded animals. The life cycle of the parasite includes an asexual reproduction in intermediate hosts (Mammals and birds) and a sexual reproduction in definitive hosts (Felidae). Cats are both the intermediate and the definitive host for T. gondii. The aim of this study was to investigate anti-T. gondii antibodies in cats by using an immunoblot method based on a major surface antigen; SAG1. Six sero-negative kittens were infected intra-peritoneally by Rh strain of T. gondii. Serum samples were collected and evaluated for the presence of IgG antibodies by using two techniques; immunoblotting and indirect fluorescent antibody test (IFAT).SAG1 based immunoblotting was able to detect anti-T. gondii antibodies at least eight days after infection. Comparative evaluation showed that this method is as sensitive as IFAT to diagnose T. gondii infection in cats.
Spontaneous Manifestation of Polycystic Kidney Disease Following Separation Anxiety in a Persian Cat
M. Hosseininejad,F. Hosseini
Pakistan Journal of Biological Sciences , 2008,
Abstract: A 4-year-old female Persian cat was referred with the history of separation from the owner. There was no clinical sign and everything had been reported as normal during the veterinary checkup just before separation and traveling. The history and clinical signs were fit to separation anxiety when the cat referred to us. Ten days after the diagnosis and starting of the treatment for separation anxiety, the cat was referred again with the clinical signs of chronic renal failure. Further assessments showed an inherited polycystic kidney disease. Spontaneous manifestation of polycystic kidney disease after separation anxiety indicate that this phenomenon may cause the chronic process of polycystic kidney disease to promote.
Antiserum production in immunized camel by the venom of Hemiscorpius lepturus scorpion: evaluation of neutralizing test in vivo
Behdani M,Hosseininejad chafi M,Zeinali S,Karimipour M
Tehran University Medical Journal , 2010,
Abstract: "nBackground: Scorpion envenomation is considered as one of the Public Health problems in some countries in the world including Iran. Annually, approximately 30,000 scorpion stings happen in Iran from which 12% belongs to Hemiscorpius lepturus (special small closely spaced, bead-shaped jointed tail, similar in the shape to a cows tail, and is locally called ‘‘gaodim'' (Gao, cow; dim, tail)) with 95% mortality. The main treatment is antiserum therapy which is produced in horse and is the only way to neutralize the venom. Due to the anaphylactic shock of the horse antiserum in some of the stung patients other source of antiserum is recommended. In this study the ability of produced camel antiserum in neutralizing the scorpion venom of Hemiscorpius lepturus was performed in Balb/c model. "n"nMethods: Camel is an animal model that genetically is compatible with human genome utilized in this research to produce antiserum against scorpion venom. Two camels were used for immunization with the venom of Hemiscorpius lepturus. ELISA method was used to confirm the immunity. Antiserum was produced and used for neutralizing test. The precipitated antiserum with saturated ammonium sulfate (SAS) was also used to perform the neutralizing test in mice. "n"nResults: The results indicated that the amount of 200 μl of antiserum and 400 μl of SAS antiserum were able to neutralize the amount of 1 LD100 of the venom and the survived the mice from death. "n"nConclusion: The result indicated that camel antiserum against scorpion venom is capable to neutralize the crude venom in mice model. Due to the safety of camel serum in human, it is suggested that the produced antiserum in camel can be substitute with the traditional horse antiserum in scorpion stung patients.
A study of the prevalence of intestinal parasitic infection in HIV positive individuals in Mashhad, Northeast Iran
Fariba Berenji,Mohammad Reza Sarvghad,Abdolmajid Fata,Zahra Hosseininejad
Jundishapur Journal of Microbiology , 2010,
Abstract: Introduction and objective: Intestinal parasitic infection is an important problem in the Human Immunodeficiency Virus (HIV)-infected patients. The aim of this study was to investigate the prevalence of intestinal parasitic infections among HIV+ patients in Mashhad, Iran.Materials and methods: A coproparasitological study was conducted from October 2005 to August 2006 at Emam Reza hospital, Mashhad University of Medical Sciences, Iran. It was carried out on 31 HIV+ patients admitted at the HIV clinic and 20 HIV-negative individuals as control group using direct and formalin-ether sedimentation concentration methods, trichrome and acid-fast staining.Results: Overall prevalence of intestinal parasites among HIV+ population was 67.7% and in control group was 55% without significant difference between the two groups. More specifically, the following parasites were identified in HIV+ group: Giardia lamblia 22.6%, Blastocystis hominis 22.6%, Chilomastics mesnili 22.6%, Entamoeba coli 9.7%, and Entromonas 3.2%. In the control group Entromonas (45%), B. hominis (15%), E. coli (10%), G. lamblia (5%), and Hymenolepis nana (10%). However, the prevalence of G. lamblia, B. hominis and C. mesnili was greater for HIV+ patients (p<0.05). There were statistically significant differences between trichrome staining (28, 54.9% positive for parasites), acid fast methods (6, 11.8%), direct method (7, 13.7%) and formalin-ether method (13, 25.5%) in detection of parasites in two groups (p< 0.05). Conclusion: Our study shows the importance of testing for intestinal parasites in patients who are HIV-positive, and emphasizes the necessity of increasing awareness among clinicians regarding the occurrence of parasite infections in these patients. Routine examination of stool samples for parasitic infections could significantly benefit the HIV-infected individuals by contributing to reduce morbidity, mortality and improved quality of life.
Seroprevalence of Toxoplasma Gondii Infection in Dogs in Tehran, Iran
M Hosseininejad,A Malmasi,F Hosseini,M Selk-Ghaffari
Iranian Journal of Parasitology , 2011,
Abstract: Background: Toxoplasma gondii infects a wide range of animals; felines are definitive hosts and other animals including the dogs are intermediate hosts. The aim of this study was to determine the seroprevalence of T. gondii infection in dogs in Tehran, capital of Iran and to investigate possi-ble associated risk factors.Methods: Three hundreds ninety six serum samples were collected during 2007-8 from the dogs. Col-lected samples were tested using an indirect fluorescent antibody test (IFAT) in dilutions of 1:16 and more. All procedures were carried out in Shahrekord University, Iran. All the data were analyzed using SPSS software, qui square test with confidence interval of 0.95.Results: From evaluated samples, 89 (22.47%) were positive in titers of at least 1:16. further evalua-tions in other dilutions showed positive results in dilutions of maximum 1:16 , 1:32, 1:64, 1:128 and 1:256 in 38, 29, 15, 2 and 5 dogs respectively. Investigation of the role of risk factors showed no sex predisposition while infection rate was significantly higher in dogs older than one year old. Living places were of significant importance; infection rate was significantly higher in stray or guard dogs in compare with household dogs (P<0.05). Conclusion: Relatively high seroprevalence of T. gondii infection in dogs in Tehran shows high environmental contamination. It is recommended that the dogs with suspected clinical signs be tested for T. gondii infection.
Seroprevalence of Neospora caninum Infection in Camels (Camelus dromedarius) in Isfahan Province, Center of Iran
M Hosseininejad,K Pirali-Kheirabadi,F Hosseini
Iranian Journal of Parasitology , 2009,
Abstract: "nBackground: The aim of this study was to investigate the sero-prevalence of Neospora caninum infec-tion in Camelus dromedarius; the most popular camel species in Iran. "nMethods: Totally, 310 serum samples were collected from camels in Isfahan Province during 2008 and tested in Shahrekord University using indirect fluorescent antibody test (IFAT) for se-rodiagnosis of anti-N. caninum IgG antibodies as a cross-sectional study. "nResults: Among evaluated serum samples, 10 (3.22%) had anti-N. caninum antibodies detectable in dilutions of 1:50 and 1:100. to rule out false positive results due to cross-reactivity of this pro-tozoan parasite with the closely related protozoan parasite Toxoplasma gondii, an IFA was also performed for T. gondii infection in dilution of 1:16 for positively reacted serum samples against N. caninum. No con-cordant infections of these two protozoan parasites were detected. "nConclusion: Presence of anti-N. caninum antibodies in camels in Iran emphasizes the necessity of fur-ther studies to detect the role of this organism as a pathogen and possibly economic impor-tance in ca-mels population.
Lymphoma in Syrian Hamster
M. Hosseininejad,O. Dezfoolian,F. Hosseini,F.R. Kabir
Pakistan Journal of Biological Sciences , 2008,
Abstract: This study describes clinical and pathologic findings in a naturally occurring lymphoma in a Syrian hamster. Lymphoma; the most common tumor of hamster is viral induced tumor with veterinary importance. Clinical signs include emaciation, weakness, lethargy, diarrhea and some rectal bleeding. The present study describes clinical and pathologic findings of lymphoma in Syrian hamster. The disease is naturally occurred.
Neospora Caninum and Leishmania Infantum Co-Infection in Domestic Dogs (Canis Familiaris) in Meshkin-Shahr District, Northwestern Iran
M Sharifdini,M Mohebali,H Keshavarz,M Hosseininejad
Iranian Journal of Arthropod-Borne Diseases , 2011,
Abstract: Background: Mediterranean visceral leishmaniasis (MVL) is an infectious disease that affects both human and ani-mals. Domestic dogs (Canis familiaris) are principal reservoir hosts of MVL caused by Leishmania infantum. Dogs are definitive hosts for Neospora caninum and a risk factor for infecting intermediate hosts. The immunosuppression caused by visceral leishmaniasis (VL) can promote the occurrence of co-infections with other agents such as neosporo-sis. This study aimed to determine the frequency of co-infection of the both protozoan parasites in the en-demic areas of VL from Meshkin-Shahr District, north-west of Iran. Methods: Altogether, 171 serum samples were collected from domestic dogs of Meshkin- Shahr District by multistage cluster sampling from October 2008 to August 2009. The collected serum samples were tested for the detection of simultaneous infection of L. infantum and N. caninum using direct agglutination test (DAT) and indirect ELISA, respectively. Results: Of the 171 domestic dogs, 27 (15.8%) and 52 (30.4%) were showed antibodies against L. infantum and N. caninum, respectively. Simultaneous infections of N. caninum and L. infantum was found in 16 (9.4%) of the dogs. In VL-positive and VL-negative dogs, N. caninum infection was found in 59.3% and 25.0%, respectively. A statisti-cally significant difference was found between VL-positive and VL-negative dogs with N. caninum infection (P= 0.001). Conclusion: These findings indicate that Meshkin-Shahr District in northwestern Iran is an active focus of canine visceral leishmaniasis (CVL). Neospora caninum and L. infantum co-infection is prevalent in the area and infection by L. infantum seems to enhance susceptibility to N. caninum infection in domestic dogs.
BLINK REFLEX IN MULTIPLE SCLEROSIS: AN ANCILLARY TEST FOR DETECTING BRAIN STEM LESIONS
M ETEMEDYFAR,M SHIRAZADI,M.R NAJAFI,M HOSSEININEJAD
Journal of Research in Medical Sciences , 2001,
Abstract: Introduction. Electrodiagnostic tests are one of the ancillary procedures that are used for diagnosis of multiple sclerosis (MS). This study investigates the frequency of abnormal blink reflex in patients with MS. Methods. In this cross sectional diagnostic study, 100 patients (26 male and 74 female) with definite MS were selected based on clinical and MRI findings. they were referred to Al- zahra hospital (affiliated to iUMSHS) during year 2000. Blink reflex (BR) waves including R1, R2, R2 were recorded inpatients through the stimulation of supraorbital nerve. Results. The frequency of abnormal BR in MS patients with brain stem involvement was 77.9 percent and in those without brain stem involvement was 36.6 percent (P < 0.001). There was a significant relationship between the duration of MS and the abnormality in BR. Discussion. The frequency of abnormal blink reflex in MS is significantly associated with site of involvement in the brain. The majority of MS patients with brain stem involvement have abnormal BR. It is proposed that in patients with symptoms and signs of MS if there was no accessibility for MRI or if the results of MRI were equivocal, blink reflex test should be performed in addition to other ancillary tests.
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