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Search Results: 1 - 10 of 120214 matches for " Yuying Wang "
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Targeting miRNAs in Osteoblast Differentiation under Malnutrition Conditions  [PDF]
Yuying Wang, Rui He, Liangjun Zhong
Journal of Biosciences and Medicines (JBM) , 2018, DOI: 10.4236/jbm.2018.65012
Abstract: Aims: Previous studies reported that reduced bone formation was identified in fasting adult female mice compared with the ad libitum control group. An increasing number of studies have shown that miRNAs contribute to bone homeostasis. Unfortunately, there are minor concerns about the underlying mechanisms in osteoblastic differentiation under malnutrition conditions. Methods: We investigated microRNAs (miRNAs) in osteoblastic differentiation under malnutrition conditions using high-throughput bioinformatics approaches. To screen for targeted microRNAs, sequences were quantified by aligning reads to miRbase using miRDeep2 software. Unadjusted p-values were calculated using the Student’s t-test. Genes with a p-value of <0.05 and log 2FC (fold change) ≥ 1 were considered differentially expressed genes (DEGs). DEGs were submitted to Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, respectively. Results: They were mainly enriched in biological process terms type and biological pathways, respectively. Particularly, we evaluated seven microRNAs, mir-494 3p, mir-466, mir-455, mir-708, mir-298, mir-92 and mir-224, which likely play roles in osteoblastogenesis in fasting adult mice. Conclusion: To our knowledge, this is the first study on the expression pattern of miRNA in osteoblasts of malnourished adult mice. These targeting miRNAs may provide a potential therapeutic approach to treat osteoporosis.
In Vitro Mass Scale Propagation of Wild Cymbidium lowianum with a Rare and Endangered Plant  [PDF]
Yuying Wang, Zhilin Li, Liping Huang, Jun Su
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.47182

The wild Cymbidium lowianum, a national-level rare and endangered species of Orchid, is an excellent garden plant with ornamental flowers with striking, deep red lips in a V-shaped formation. The objective of this study was to establish a micropropagation protocol system via immature seeds of wild Cymbidium lowianum, evaluate the Murashige and Skoog (MS), half-strength Murashige and Skoog (1/2 MS) medium, 6-benzylaminopurine (BA), a-napthaleneacetic acid (NAA), organic additions activated charcoal (AC) and banana pulp (BP) effects on the different morphogenesis (seed germination, multiple shoot and rooting) in vitro. The optimal combination for the germination of seed was 1.0 mg·L-1 BA with 0.5 mg·L-1 NAA in 1/2 MS, and addition 0.3%AC, which resulted in 95% seed germination in 90 days. The best formulation for multiple shoot was 1/2 MS medium containing 2.5 mg·L-1 BA, 0.5 mg·L-1 NAA and addition 8% BP in which produced 19.8 shoots per protocorm in 60 days. Multiple shoots were cut and

2-(Ethoxycarbonyl)quinolinium butyltrichlorido(quinoline-2-carboxylato-κ2N,O)stannate(IV)
Hongyun Wang,Handong Yin,Yuying Sun
Acta Crystallographica Section E , 2008, DOI: 10.1107/s1600536807067566
Abstract: In the title compound, (C12H12NO2)[Sn(C4H9)(C10H6NO2)Cl3], the Sn atom is coordinated by one O and one N atom of a quinolinium-2-carboxylate anion, three Cl atoms and one butyl ligand within a distorted octahedron. Three C atoms of the butyl ligand are disordered and were refined using a split model; the site occupancies are 0.57 and 0.43. The NH group of the cation is involved in intermolecular N—H...O hydrogen bonding.
Hongyun Wang,Handong Yin,Yuying Sun
Acta Crystallographica Section E , 2008, DOI: 10.1107/s1600536807063623
Abstract: The title compound, [Sn(C4H9)3(C6H5O2S)]n, possesses an infinite chain structure. The SnO2C3 centre has a distorted trigonal-bipyramidal geometry (τ = 0.145) with the O atoms in the axial positions. Atoms of the thiophene group S1 and C4 are disordered over two sites The S atom and one C atom, with attached H atom, of the thiophene ring are disordered over two positions; the site occupancy factors are ca 0.7 and 0.3.
Single-molecule studies of DNA by molecular combing
Liu Yuying,Wang Pengye,Dou Shuoxing,
Liu Yuying
,Wang Pengye and Dou Shuoxing

自然科学进展 , 2007,
Effect of Carotid Artery Stenting and Extracranial-Intracranial By-Pass on Cognitive Function: Preliminary Results  [PDF]
Li Liu, Yuying Zhou
International Journal of Clinical Medicine (IJCM) , 2015, DOI: 10.4236/ijcm.2015.66049
Background: To compare the neuropsychological consequences after carotid artery stenting (CAS) and extracranial-intracranial by-pass (EC-IC by-pass). Methods: A total of 43 patients referred to CAS, 32 patients referred to EC-IC by-pass and 43 control subjects were enrolled in the study. Neuropsychologic testing was performed before and three months after procedure. A paired Student t test was used to compare neuropsychologic test scores at baseline and three months after procedure in each group. Cognitive changes in a three-month follow-up were not normally distributed and compared among/between groups with Kruskal-Wallis test. Results: Three months after the treatment both the CAS and EC-IC by-pass groups showed improved cognitive performance compared to baseline, whereas the same improvement wasn’t seen in the control group. The scores from Activities of Daily Living also improved in all three groups in a three-month follow-up, and EC-IC group presented a more distinct increase in daily life abilities comparing to the other two groups. Conclusions: CAS and EC-IC by-pass in patients with a carotid or intracranial stenosis may result in cognitive improvement three months after surgery.
Evaluate Laser Needle Effect on Blood Perfusion Signals of Contralateral Hegu Acupoint with Wavelet Analysis
Guangjun Wang,Yuying Tian,Shuyong Jia,Gerhard Litscher,Weibo Zhang
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/103729
Abstract: Our previous studies suggested that the MBF in contralateral Hegu acupoint (IL4) increased after ipsilateral Hegu acupoint was stimulated with manual acupuncture. In this study, twenty-eight (28) healthy volunteers were recruited and were randomly divided into Hegu acupoint stimulation group and Non-Hegu stimulation group. All subjects received the same model stimulation of the laser needle for 30 min in right Hegu acupoint and Non-Hegu acupoint, respectively. MBF of left LI4 was measured by the laser Doppler perfusion imaging system. The original data dealt with morlet wavelet analysis and the average amplitude and power spectral density of different frequency intervals was acquired. The results indicated that right Hegu stimulation with the laser needle might result in the increase of left Hegu acupoint MBF. 40 min later after ceased stimulation, the MBF is still increasing significantly, whereas the MBF has no significantly change in Non-Hegu stimulation group. The wavelet analysis result suggested that compared to Non-Hegu stimulation, stimulated to right Hegu acupoint might result in the increase of average amplitude in frequency intervals of 0.0095–0.02 Hz, 0.02–0.06 Hz, and 0.06–0.15 Hz, which might be influenced by the endothelial, neurogenic, and the intrinsic myogenic activity of the vessel wall, respectively.
Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea
Tracy Chong, Joel M Stary, Yuying Wang, Phillip A Newmark
BMC Developmental Biology , 2011, DOI: 10.1186/1471-213x-11-69
Abstract: To identify genes expressed differentially between these strains, we performed microarray analyses and identified >800 genes that were upregulated in the sexual planarian. From these, we characterized 24 genes by fluorescent in situ hybridization (FISH), revealing their expression in male germ cells or accessory reproductive organs. To identify additional markers of the planarian reproductive system, we also used immuno- and fluorescent lectin staining, identifying several antibodies and lectins that labeled structures associated with reproductive organs.Collectively, these cell-type specific markers will enable future efforts to characterize genes that are important for reproductive development in the planarian.While the planarian has re-emerged as an animal model for studying regeneration and stem cell biology [1-5], recent studies of Schmidtea mediterranea have also contributed to understanding germ cell biology and sexual development [6-9]. Two modes of specifying germ cells have evolved among animals, involving either localized determinants or inductive signaling [10-12]. Sexual planarians have a reproductive system that can be regenerated de novo from stem cells after amputation [9,13], suggesting that they specify their germline inductively like mammals and many basal metazoans [10,14,15].S. mediterranea is a powerful model for understanding germ cell development due, in part, to the existence of distinct sexual and asexual strains that allow identification and characterization of genes involved in the different modes of reproduction. The reproductive system of the hermaphrodite consists of the male and female gonads, as well as accessory reproductive organs [7]. Numerous testes are distributed dorsolaterally along the animal, and a pair of ovaries is situated more ventrally at the posterior region of the cephalic ganglia (brain). Ciliated oviducts and sperm ducts running along the nerve cords lead to the copulatory apparatus, comprised of the gonopore (gn),
hB1F and HNF1 work synergistically in the regulation of the function of HBV EN II
Yanning Cai,Mei Li,Qing Zhou,Yuying Kong,Yuan Wang
Chinese Science Bulletin , 2001, DOI: 10.1007/BF03187008
Abstract: Human B1 binding factor (hB1F) is a novel transcription factor cloned in our laboratory, which has the capacity of binding the Bl region of HBV enhancer II (EN II) and stimulating its activity. In this report, the functional relationship between hB1F and other liver-enriched activators of EN II was investigated. The results of CAT assay indicated that there was only additive effect between hB1F and HNF4 as well as HNF3β. On the contrary, the presence of both hB1F and HNF1 would result in significant functional synergism. Further GST pull down assay indicated that hB1F could interact with HNF1 directlyin vitro. This study provides the evidence that hB1F and HNF1 work synergistically in the regulation of the activity of HBV EN II.
DNA-based immunization against hepatitis B surface antigen carrying preS epitopes
Jingyi Hui,Guangdi Li,Yuying Kong,Yuan Wang
Chinese Science Bulletin , 1999, DOI: 10.1007/BF03182722
Abstract: Three plasmid expression vectors containing modified hepatitis B surface antigen (HBsAg) carrying preS epitopes were constructed. Transient expression afterin vitro transfection in COS-M6 cells showed that under the transcriptional control of the human cytomegalovirus (CMV) immediate early promoter, fusion genes expressed the modified HBV envelope proteins which were efficiently secreted into culture medium and presented HBsAg, preS1 and preS2 antigenicity. DNA-based immunization with these plasmids carrying preS sequences induced anti-HBs antibody in BALB/c mice. The titers of anti-HBs antibody were higher than those appeared in mice immunized with plasmid carrying S gene only. DNA injection with plasmids containing preSl sequences elicited also high titers of anti-preS1 antibody. Moreover, the antipreSl antibodies were found to appear earlier than anti-HBs antibodies.
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