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Search Results: 1 - 10 of 1889 matches for " Yunnan Key Laboratory of Sugarcane Genetic Improvement "
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Phenotypic diversity of sugarcane variety germplasm

Xinlong Liu,Qing Cai,Caiwen Wu,Li Ma,Xiongmei Ying,Xin Lu,Yuanhong Fan,Yunnan Key Laboratory of Sugarcane Genetic Improvement,Kaiyuan,Yunnan Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Biotechnology & Genetic Resources Institute,Kunming,

生物多样性 , 2010,
Abstract: Sugarcane varieties are important in providing parental resources in sugarcane breeding programs.In order to improve efficiency in selecting sugarcane varieties as parents,to provide guidance in choosing the best parent combinations for crossing,and to assist in choosing core collections of parents,the genetic variation,genetic structure and relationships of a range of traits were studied in a set of sugarcane varieties from different origins.Seventeen qualitative traits and five quantitative traits were st...
Functional genomics of maize submergence tolerance and cloning of the related gene Sicyp51
TANG Wanhu ZHANG Zuxin,ZOU Xiling ZHENG Yonglian,
TANG Wanhu
,ZHANG Zuxin,ZOU Xiling & ZHENG Yonglian . National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan,China,. College of Life Science,Yangtze University,Jingzhou,China

中国科学C辑(英文版) , 2005,
Abstract: In this study, SSH (Suppression Subtractive Hybridization) and cDNA microarray were used to identify genes associated with waterlogging response of maize roots. Mo17 and Hz32 are two maize inbred lines with differential tolerance to hypoxia. Seedlings of the inbred lines with two leaves were submerged in hypoxia buffer. SSH libraries were constructed with cDNA samples from roots. Both forward and reverse subtractions were performed for each inbred line, and 105 positive clones induced by hypoxia were selected by differential screening. The treated and control message RNA were hybridized with the cDNA microarray of Mo17, sequentially, 57 of 3-fold differentially expressed clones were obtained. A total of 162 positive clones were all sequenced. Bioinformatics analysis showed these positive clones represent 85 TUGs, including genes involved in several biochemistry pathways, such as glycolysis, protection, signal transduction, cell construction and energy metabolism and 41 EST with unknown function. Comparison between Mo17 and Hz32 indicates that genes related to hypoxia tolerance have different expression patterns in submerged roots. Several positive clones' expression patterns were revealed by Northern or RT-PCR, and a new gene (Sicyp51), which may contribute to hypoxia tolerance, was identified.
Microscopic observation on mycorrhiza of Doritis pulcherrima

CHEN Jin-Hua HU Mei-Jiao SONG Xi-Qiang,HE Ming-Gao LUO Yi-Bo Key Laboratory of Tropical Horticultural Plant Resources,Genetic Improvement Affiliated to Ministry of Education,Hainan University,Danzhou,China Environment,Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,China State Key Laboratory of Systematic,Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing,China,

菌物学报 , 2010,
Abstract: 兰科菌根菌对兰花种子萌发和原球茎生长起了重要作用,在成年阶段也扮演着重要角色(即使该兰花含有叶绿素)。为了解东南亚特有种五唇兰的菌根形态和菌根真菌的动态侵染过程,对不同生境下两种变异类型叶背绿色和叶背紫色的成年植株菌根分三个时间段采样并进行显微观察。结果表明,成年的五唇兰植株与菌根真菌有着密切关系,菌丝的侵染、定殖和消解程度随着生长发育变化而变化;在营养生长阶段和生殖生长阶段都发现菌丝结,这两个阶段的菌丝特征有所不同;而在休眠阶段的菌根中观察不到菌丝结;不同生境和不同变异类型的菌根结构无明显区别。研究结果为附生兰、地生兰和腐生兰的菌根结构的比较提供了参考。
Effect of anions on hydrogen fermentation in a mixed anaerobic culture system

MA Huan,LIU Weiwei,LIU Shiqing,School of Energy & Environmental Science,Yunnan Normal University,Kunming Key Laboratory of Ion Beam Bioengineering,

环境科学学报 , 2009,
Abstract: In the present study, an L27 (313) orthogonal test was performed in order to investigate the individual and interactive effects of six anions and urea on biohydrogen production in a mixed anaerobic cultures system. Certain components were found to promote hydrogen production, including SO2-4 1.5 g L-1, Cl- 0 g·L-1, NO-3 0 g·L-1, Mo7O6-24 0.1 g·L-1, C2O2-4 1 g·L-1, C6H5O3-7 5 g·L-1, and (H2N)2CO 0 g·L-1. Verification experiments demonstrated that the maximal and the average hydrogen content in the mixed anaerobic cultures increased by 21.4% and 24.1% in presence of the above components. The average hydrogen generation rate and hydrogen yield increased from 36.79 mL·L-1·h-1 to 96.25 mL·L-1·h-1 and from 0.76 mol·mol-1 to 1.17 mol·mol-1, respectively. The results also showed that urea significantly inhibited hydrogen generation, while Mo7O6-24 enhanced the sugar digestibility of the anaerobic microorganisms. Interestingly, the interactive effect of C2O2-4 and C6H5O3-7 improved hydrogen production. Finally, the possible mechanisms underlying the interactive effects of these anions and urea are discussed.
Primary investigation on GISH-NOR in cotton
LIU Sanhong,WANG Kunbo,SONG Guoli,WANG Chunying,LIU Fang,LI Shaohui,ZHANG Xiangdi & WANG Yuhong Key Lab of Cotton Genetic Improvement of Ministry of Agriculture,Cotton Research Institute of Chinese Academy of Agricultural Sciences,Anyang,China,

科学通报(英文版) , 2005,
Abstract: Six loci of nucleolar organizer region (NOR) were detected in genomic in situ hybridization (GISH) of cotton (Gossypium). NOR was the characteristic of 45S rDNA but could be generated by genomic DNA (gDNA) extracted from Gossypium species as probe. With twice FISH to the same mitotic cell of G. herbaceum or G hirsutum, number, position and size for NORs generated from 45S rDNA and gDNA were identified largely similar or even the same. The NORs with gDNA as probe were therefore permanently defined as GISH-NORs. GISH-NORs from G hirsutum and G raimondii mitotic images were all terminal types. Four and two GISH-NORs from G herbaceum (var. africanum) were terminal and centromere types, respectively. Six GISH-NORs in G hirsutum were chromosome mapped with two in A- and four in D-subgenomes. There were also GISH-NORs in mitotic image of G raimondii with its own gDNA as probe. From mitotic image of G herbaceum with its own gDNA as probe, GISH-NOR could not be observed but non-whole-recovery of hybridized signals was distinguished. These non-whole-recovery of hybridized signals were detected on long arm terminals of most chromosomes and especially existed in nearly half long arm of a pair of chromosomes in G herbaceum gDNA probed itself GISH image, which may be possibly induced by low copy genes within the regions rather than inter-subgenomic segment translocations. GISH-NORs in G hirsutum mitotic images were dominantly observed when gDNAs from D and A genome species were used as probes and block, respectively, but not when the reverse probe and block gDNA from the two diploid progenitor genomes were designed. There may be two speculations to this special phenomenon: rDNA concerted evolution; content of rDNA in genome D more than genome A.
The current status and perspectives of on-farm conservation of crop genetic diversity

LU Bao Rong,ZHU You Yong,WANG Yun Yue Ministry of Education Key Laboratory for Biodiversity Science,Ecological Engineering,Institute of Biodiversity Science,Fudan University,Shanghai Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management,Key Laboratory of Plant Pathology,Yunnan Agricultural University,Kunming,

生物多样性 , 2002,
Abstract: Effective conservation of traditional crop varieties is the basis of the long term utilization of agricultural biodiversity, and will be one of the essential factors for the sustainable development of agriculture. Due to the intensive production practices in modern agriculture, a great number of traditional crop varieties have been rapidly replaced by the improved high yielding varieties, which has caused severe "genetic erosion" in the genepool of crop species, and significantly reduced genetic diversity of crop varieties. It is therefore essential to preserve the shrinking crop biodiversity before it is completely lost. On farm conservation is an important and dynamic in situ conservation method that conserves crop varieties in agricultural ecosystems. On farm conservation can be defined as the continued cultivation and management of a diverse set of crop populations by farmers in the agro ecosystems where a crop has evolved, and it allows the evolution of conserved crop varieties to be continued in their original habitats. However, in spite of the increasing interests in this method and a relatively large number of research and case studies reported in this field, there has been no significant achievement for on farm conservation and many questions remain unanswered: Is on farm conservation practical and achievable? Can on farm conservation actually be conducted in modern agriculture? Is on farm conservation a trade off with agricultural development? What are the scientific issues for on farm conservation? What incentives can their be for farmers to conserve genetic diversity in their farming ecosystems? All these questions need to be addressed scientifically with satisfaction when conducting effective on farm conservation. It is therefore necessary to have a better understanding of on farm conservation, to study mechanisms and scientific basis of on farm conservation, and to be innovative to open a new dimension for on farm conservation action. The biodiverse deployment of rice varieties, mixed planting of modern hybrid rice with traditional rice varieties in Yunnan Province, not only provided an excellent method of controlling rice disease and increasing rice productivity, but also demonstrated a powerful model for conserving traditional rice varieties in an effective and sustainable way. This model of mixed planting of a diverse set of crop varieties links farmer's long term benefit, socioeconomic concerns, and the actual conditions of locally based agro ecosystems appropriately with conservation activities. This model of using biodiversity to preserve biodiversity of crop varieties is a long term approach that will be sustainable and beneficial to farmers whose general living standard will be raised. It might provide possible solution for on farm conservation of traditional rice varieties in a feasible and sustainable way., and probably has potential for other crop species, too.
The function of histone chaperones during development

ZHAO Zhan-Ke,WANG Yu-Feng Hubei Key Laboratory of Genetic Regulation,Integrative Biology,College of Life Science,Huazhong Normal University,Wuhan,China,

遗传 , 2010,
Abstract: Histone chaperones may participate the decondensation and assembly of chromatins, thus regulate gene expression. They play important roles in almost all developmental processes, such as gametogenesis, fertilization, embryogenesis, growth and senescence. In this review, we used well studied examples to illustrate various functions of histone chaperones during developmental processes. Focus is given to nucleoplasmin, CAF-1, HIRA, ASF1/CIA, and NAP1.
Construction of a novel kind of expression plasmid by ho-mologous recombination in Saccharomyces cerevisiae
Construction of a novel kind of expression plasmid by homologous recombination in Saccharomyces cerevisiae

CHEN Xiangling,YUAN Hanying,HE Wei,HU Xianghua,LU Hong,LI Yuyang,
CHEN Xiangling
,YUAN Hanying,HE Wei,HU Xianghua,LU Hong & LI Yuyang State Key Laboratory of Genetic Engineering,School of Life Sciences,Fudan University,Shanghai,China

中国科学C辑(英文版) , 2005,
Abstract: Based on a previously used plasmid pHC11, a new plasmid pHC11R was constructed. Cutting plasmid pHC11R with proper restriction enzymes, the resulting larger DNA fragment pHC11R' was co-transformed with a PCR amplified expression cassette of human IFNalpha2b into yeast. By means of the homologous sequences at both ends of two DNA fragments, a novel expression plasmid pHC11R-IFNalpha2b was formed via homologous recombination in the yeast. Compared with pHC11-IFNalpha2b, the expression plasmid pHC11R-IFNalpha2b was smaller in size and in absence of antibiotic resistant gene. The stability and copy number of pHC11R-IFNalpha2b were greatly increased and the expression level of heterologous protein was improved. As the derivatives of pHC11R, a series of recombination expression vectors pHRs containing different combination of expression elements were developed. This led to a rapid and powerful method for cloning and expressing of different genes in yeast.
Dynamics of tree species composition and community structure of a tropical seasonal rain forest in Xishuangbanna,Southwest China

HU Yuehua,CAO Min,LIN Luxiang Key Laboratory of Tropical Forest Ecology,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Mengla,Yunnan,China Graduate University of Chinese Academy of Sciences,Beijing,China,

生态学报 , 2010,
Abstract: 研究了西双版纳热带季节雨林1 hm2 (hectare) 动态监测样地1993年与2007年之间树种组成和群落结构的变化。对样地中胸径≥5 cm 的乔木进行了每木调查。目前其树种组成的热带分布科、属所占比例分别为91% 和94%,具有较高比例的热带植物区系性质。在1993年与2007年两次调查之间,树种数量由145种增至179种,仅有1到2个个体的稀有树种所占比例从54% 降为51.1%。从森林的垂直结构来看,A、B、C 三层的个体死亡率分别为12.8%、12.9% 和19.0%,各层树木的增长率分别为-8.5%、-1.4% 和44.8%。与此相对应,C 层小径级的树木所占比例有较大提高。虽然小径级的树木在种类和数量上比例增大,但个体数量和种类组成相对稳定的 A、B 层优势树种变化不大,维持了群落结构的稳定性。14a间,群落中新增加的具有先锋性质的树种不超过5个。1993年时,A、B 两层尚有先锋树种存在,2007年已经从 A、B 两层中退出。因此,从14a间树种组成和群落结构的变化来看,虽然具有树木的死亡和增补,但其物种成分和群落结构的总体格局没有明显的变化,处于动态平衡过程中。
Relationship of Microsorium membranaceum var. carinatum and Microsorium membranaceum var. membranaceum based on the sequence analysis of chloroplast rbcL , rps4 ,trnL-trnF and rps4-trnS

Chunxiang Li,Shugang Lu State Key Laboratory of Palaeobiology,Stratigraphy of Nanjing Institute of Geology,Palaeontology,Chinese Academy of Sciences,Nanjing Institute of Ecology,Geobotany,Yunnan University,Kunming,

生物多样性 , 2005,
Abstract: Microsorium membranaceum var. carinatum , a newly published variety, can be distinguished easily from the original variety M. membranaceum var. membranaceum by the stipe morphology. The cross section of stipes is triangular in the former, but semicircular in the latter. Here phylogenetic relationship of the two varieties was investigated based on the sequence analysis of chloroplast rbcL , rps4 , trnL trnF , and rps4 trnS intergenic spacers. Furthermore, the published relevant sequences from other ferns were also analyzed for the comparison of genetic distance. As a result, no sequence difference in the studied four cpDNA regions was found between M. membranaceum var. carinatum and M. membranaceum var. membranaceum , but some difference in the corresponding DNA fragments is present among other fern varieties. Hence, the status of Microsorium membranaceum var. carinatum as a distinct variety is not supported by the DNA analysis.
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