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Search Results: 1 - 10 of 1337 matches for " Yuji Yasukochi "
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Sex-Linked Pheromone Receptor Genes of the European Corn Borer, Ostrinia nubilalis, Are in Tandem Arrays
Yuji Yasukochi,Nami Miura,Ryo Nakano,Ken Sahara,Yukio Ishikawa
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0018843
Abstract: Tuning of the olfactory system of male moths to conspecific female sex pheromones is crucial for correct species recognition; however, little is known about the genetic changes that drive speciation in this system. Moths of the genus Ostrinia are good models to elucidate this question, since significant differences in pheromone blends are observed within and among species. Odorant receptors (ORs) play a critical role in recognition of female sex pheromones; eight types of OR genes expressed in male antennae were previously reported in Ostrinia moths.
Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths: A Useful Resource for Comparative Genomics of Lepidoptera
Yuji Yasukochi,Makiko Tanaka-Okuyama,Manabu Kamimura,Ryo Nakano,Yota Naito,Yukio Ishikawa,Ken Sahara
Journal of Biomedicine and Biotechnology , 2011, DOI: 10.1155/2011/165894
Abstract: Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, =31, which are not closely related with each other or with the silkworm, Bombyx mori, (=28), the sequenced model lepidopteran. A total of 108–184 clones representing 101–182 conserved genes were isolated for each species. For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species. The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.
Extensive Conserved Synteny of Genes between the Karyotypes of Manduca sexta and Bombyx mori Revealed by BAC-FISH Mapping
Yuji Yasukochi,Makiko Tanaka-Okuyama,Fukashi Shibata,Atsuo Yoshido,Franti?ek Marec,Chengcang Wu,Hongbin Zhang,Marian R. Goldsmith,Ken Sahara
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0007465
Abstract: Genome sequencing projects have been completed for several species representing four highly diverged holometabolous insect orders, Diptera, Hymenoptera, Coleoptera, and Lepidoptera. The striking evolutionary diversity of insects argues a need for efficient methods to apply genome information from such models to genetically uncharacterized species. Constructing conserved synteny maps plays a crucial role in this task. Here, we demonstrate the use of fluorescence in situ hybridization with bacterial artificial chromosome probes as a powerful tool for physical mapping of genes and comparative genome analysis in Lepidoptera, which have numerous and morphologically uniform holokinetic chromosomes.
On the Generalization of Hilbert’s 17th Problem and Pythagorean Fields  [PDF]
Yuji Shimizuike
Advances in Pure Mathematics (APM) , 2013, DOI: 10.4236/apm.2013.37A001

The notion of preordering, which is a generalization of the notion of ordering, has been introduced by Serre. On the other hand, the notion of round quadratic forms has been introduced by Witt. Based on these ideas, it is here shown that 1) a field F is formally real n-pythagorean iff the nth radical, RnF is a preordering (Theorem 2), and 2) a field F is n-pythagorean iff for any n-fold Pfister form ρ. There exists an odd integer l(>1) such that l×ρ is a round quadratic form (Theorem 8). By considering upper bounds for the number of squares on Pfister’s interpretation, these results finally lead to the main result (Theorem 10) such that the generalization of pythagorean fields coincides with the generalization of Hilbert’s 17th Problem.

Observation of Environmental Stress Cracking in Polymethylmethacrylate by Using the Chemiluminescence Method  [PDF]
Yuji Higuchi
Materials Sciences and Applications (MSA) , 2015, DOI: 10.4236/msa.2015.611107
Abstract: Polymethylmethacrylate (PMMA) is highly regarded for its transparency, and is used in such products as cameras and Video Tape Recorders as plastic lenses to take advantage of its excellent optical properties. Also, it is used in numerous other industrial fields like automobile lamp lenses, billboards, and lighting equipment. The phenomenon of environmental stress cracking is known to occur in PMMA due to ethanol, and there are cases when this may become a factor which causes damage of molded products. In the present paper, upon close observation by using the method of chemiluminescence in order to elucidate the mechanism by which this environmental stress cracking occurs, we report that we are able to capture the formation of a radical at the moment of cracking.
The Change in Self-Esteem among Middle School Students in Japan, 1989-2002  [PDF]
Yuji Ogihara
Psychology (PSYCH) , 2016, DOI: 10.4236/psych.2016.711136
Abstract: This paper examined temporal changes in self-esteem among middle school students in Japan from 1989 to 2002. Previous research showed that self-esteem decreased among middle school students between 1999 and 2006. However, it was unclear whether such a decrease was also present in other periods of time. To obtain a better understanding of how people adapt to socio-economic environments, it is important to gain a more comprehensive view of such long-term cultural/social changes. Therefore, to supplement the findings of the existing research on changes in self-esteem, we analyzed large-sample time-series data collected in Japan in 1989 and 2002. We found that middle schoolers’ self-esteem decreased from 1989 to 2002, consistent with previous research. Thus, our study extends the previous research by presenting additional data showing the decrease in self-esteem in Japan.
Rheological Behavior of Nanosilica Suspensions in Poly(Ethylene Oxide) Solutions with Sodium Chloride  [PDF]
Yuji Hirose
Materials Sciences and Applications (MSA) , 2017, DOI: 10.4236/msa.2017.85022
Abstract: Suspensions of silica nanoparticles showed shear-thickening profiles under steady shear conditions up on addition of a small amount of poly(ethylene oxide) (PEO). The suspensions turned into gels upon shaking and their fluidity was recovered several minutes after resting. We studied the rheological properties of these shake gels with small amounts of sodium chloride (NaCl). Gelation occurred at lower shear rates upon addition of small amounts of NaCl. In addition, the time taken by the gelated samples to recover their original viscosity increased with the increasing NaCl content. The weakened repulsive interactions between the silica particles upon NaCl addition lead to particles in closer proximity, and three-dimensional networks of PEO chains are easily formed as the electric double layer of the particles becomes thinner.
Orbital Phase Theory and the Diastereoselectivity of Some Organic Reactions  [PDF]
Yuji Naruse
Journal of Materials Science and Chemical Engineering (MSCE) , 2018, DOI: 10.4236/msce.2018.67005
The orbital phase refers to the relationship between orbitals that originates from their wave character. We show here that the orbital phase essentially determines the diastereoselectivity of the following three organic reactions. 1) Torquoselectivity of the electrocyclicring-opening reaction of 3-substituted cyclobutenes; 2) Contradictory torquoselectivity of the retro-Nazarov reaction; 3) Diastereoselectivity in electrophilic addition to substituted ethylenes.
Effect of Collagen Type I or Human Fibronectin on Imatinib Cytotoxicity in Oral Squamous Cell Carcinoma  [PDF]
Masahiko Morioka, Mai Hazekawa, Tomoyo Kawakubo-Yasukochi, Takuya Nishinakagawa, Seiji Nakamura, Manabu Nakashima
Pharmacology & Pharmacy (PP) , 2016, DOI: 10.4236/pp.2016.77032
Abstract: Extracellular matrix (ECM) components are critical for all aspects of cell proliferation, adhesion, and morphological alteration. Recent progress has yielded multiple molecular drugs that specifically target gene products which are expressed at high levels in tumor cells. We investigated whether the sensitivity of tumor cells to molecular target drugs could be altered when cells were cultured on surfaces with various coating conditions such as lysine, laminin, Matrigel, collagen type I, and human fibronectin (HFN). This study evaluates the IC50 values of imatinib in oral squamous cell carcinoma (OSCC) cell lines when cells are cultured on plates coated with ECM components such as collagen type I and HFN. Four OSCC cell lines—SQUU-A, SQUU-B, SAS, and NA— are used. Cell proliferation was assessed using WST-8 reagent. Collagen type I and HFN significantly enhanced OSCC cell proliferation compared with control. Imatinib cytotoxicity was demonstrated following culture of OSCCs in culture plates coated with collagen type I or HFN. However, there were no significant changes in imatinib IC50 values between collagen type I and HFN. These results indicate that some molecular target drugs exhibit cancer cell cytotoxicity without being influenced by cell environment factors such as the ECM. These results may aid in the search for molecular target drugs to apply in the clinical chemotherapy of OSCC.
Estimation of Growth Area of Aquatic Macrophytes Expanding Spontaneously in Lake Shinji Using ASTER Data  [PDF]
Yuji Sakuno, Hidenobu Kunii
International Journal of Geosciences (IJG) , 2013, DOI: 10.4236/ijg.2013.46A1001

In this study, we estimated the growth area of aquatic macrophytes that have expanded spontaneously in Lake Shinji, located in eastern Shimane Prefecture, Japan, using Terra satellite Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) data. Visible and near infrared ASTER data from April, August, and September 2012 were used. The water depth at which ASTER can detect submersed aquatic macrophytes using in situ spectral reflectance of aquatic macrophytes and a bio-optical model was also examined. As a result, when the threshold value of a normalized difference vegetation index (NDVI) was set to 0, only aquatic macrophytes up to a depth of approximately 10 cm could be detected. The growth area of aquatic macrophytes detected by NDVI from ASTER data was in relatively good agreement with the growth area as observed by aerial photography.

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