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Search Results: 1 - 10 of 16452 matches for " Yuhua Qi "
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Evaluating China’s Oil Security and Overseas Oil Investment  [PDF]
Yuhua Zheng
American Journal of Industrial and Business Management (AJIBM) , 2017, DOI: 10.4236/ajibm.2017.78069
Abstract: Oil security is the major concern of China’s energy policy due to the no effective substitute nature of oil and the heavy reliance on internationally imported crude oil. In this paper, a synthesized indicator system was established to analyze China’s oil security from four aspects and Chinese government’s oil security policies were also discussed. From this analysis, we concluded that the main risk in China’s oil security was the gaps between domestic oil supply and demand, and the main means that Chinese government employed on securing oil safety was oil importing diversification, improving transnational pipeline conditions, and enhancing stable domestic oil production. Furthermore, the multiple roles of overseas equity oil production from Chinese National Oil Companies in securing oil safety were discussed and the possible measures and policies on further improving oil safety were also suggested.
Altered serum microRNAs as biomarkers for the early diagnosis of pulmonary tuberculosis infection
Qi Yuhua,Cui Lunbiao,Ge Yiyue,Shi Zhiyang
BMC Infectious Diseases , 2012, DOI: 10.1186/1471-2334-12-384
Abstract: Background Pulmonary tuberculosis (TB) is a highly lethal infectious disease and early diagnosis of TB is critical for the control of disease progression. The objective of this study was to profile a panel of serum microRNAs (miRNAs) as potential biomarkers for the early diagnosis of pulmonary TB infection. Methods Using TaqMan Low-Density Array (TLDA) analysis followed by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) validation, expression levels of miRNAs in serum samples from 30 patients with active tuberculosis and 60 patients with Bordetella pertussis (BP), varicella-zoster virus (VZV) and enterovirus (EV) were analyzed. Results The Low-Density Array data showed that 97 miRNAs were differentially expressed in pulmonary TB patient sera compared with healthy controls (90 up-regulated and 7 down-regulated). Following qRT-PCR confirmation and receiver operational curve (ROC) analysis, three miRNAs (miR-361-5p, miR-889 and miR-576-3p) were shown to distinguish TB infected patients from healthy controls and other microbial infections with moderate sensitivity and specificity (area under curve (AUC) value range, 0.711-0.848). Multiple logistic regression analysis of a combination of these three miRNAs showed an enhanced ability to discriminate between these two groups with an AUC value of 0.863. Conclusions Our study suggests that altered levels of serum miRNAs have great potential to serve as non-invasive biomarkers for early detection of pulmonary TB infection.
Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
Cui, Lunbiao;Ge, Yiyue;Qi, Yuhua;Shi, Zhiyang;Jiao, Yongjun;Qi, Xian;Zhai, Xiangjun;Wang, Hua;
Brazilian Journal of Microbiology , 2010, DOI: 10.1590/S1517-83822010000200036
Abstract: the pres1 region of the l protein is important in cell attachment and consequently in hepatitis b virus (hbv) infectivity. to identify novel pres1 interacting protein, we performed glutathione-s-transferase (gst) pull-down, two-dimensional gel electrophoresis (2-de) and mass spectrometry assays. glucose-regulated proteins (grp) 78 and 75 were found to bind pres1. the interactions between pres1 and grp75 were confirmed by a co-immunoprecipitation experiment. grp78 and grp75 may play important roles in mediating hbv virion entering into hepatocyte and regulating proper folding of the l protein due to their critical functions in protein folding and trafficking. the finding of novel pres1 binding protein enriches our knowledge about molecular mechanism of hbv infection.
Development and Evaluation of a SYBR Green-Based Real Time RT-PCR Assay for Detection of the Emerging Avian Influenza A (H7N9) Virus
Zheng Zhu, Huan Fan, Xian Qi, Yuhua Qi, Zhiyang Shi, Hua Wang, Lunbiao Cui, Minghao Zhou
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0080028
Abstract: Most recently a novel avian-origin influenza A (H7N9) virus emerged in China and has been associated with lots of human infection and fatal cases. Genetic analysis of the viral genome revealed that this reassortant virus might be better adapted to humans than other avian influenza viruses. Molecular diagnostic methods are thus urgently needed in public health laboratories. In this study, a SYBR green-based one-step real time reverse transcription-PCR (RT-PCR) was developed to detect the novel H7N9 virus. The primer pairs on the basis of the hemagglutinin and neuraminidase gene sequences of H7N9 viruses amplified subtype-specific fragments with Tm values of 80.77±0.06°C for H7 and 81.20±0.17°C for N9 respectively. The standard curves showed a dynamic linear range across 6 log units of RNA copy number (106 to 101 copies/ μl) with a detection limit of 10 copies per reaction for both H7 and N9 assays by using serial ten-fold diluted in-vitro transcribed viral RNA. In addition, no cross-reactivity was observed with seasonal H1N1, H1N1 pdm09, H3N2, H5N1 and H9N2 viruses as well as other human respiratory viruses. When the assay was further evaluated in H7N9 virus infected clinical samples, positive amplification signals were obtained in all of the specimens with the accordance between H7 and N9 assays. Therefore, the established SYBR green-based real time RT-PCR assay could provide a rapid, sensitive, specific and reliable alternative approach with lower costs for high throughput screening of suspected samples from humans, animals and environments in first line public health laboratories.
Issues and Challenges in Implementing China’sGreen Public Procurement Program  [PDF]
Yuhua Qiao, Conghu Wang
Journal of Environmental Protection (JEP) , 2011, DOI: 10.4236/jep.2011.28119
Abstract: Sustainable development and environmental protection are the themes of the day. One popular policy tool that government uses to promote sustainable development and to protect the environment is green public procurement. Chinese government established its public procurement system in the late 1990s. It has moved to implement green procurement since 2004. In this paper, the authors will first trace the development of Chinese green public procurement program. The authors will then examine the issues involved in its implementation and make suggestions as to how to make green public procurement program more effective. This is one of the first papers examining China’s green public procurement program.
Notes on the Global Attractors for Semigroup  [PDF]
Lan Xu, Yuhua Shi
International Journal of Modern Nonlinear Theory and Application (IJMNTA) , 2013, DOI: 10.4236/ijmnta.2013.24030
Abstract:

First we introduce two necessary and sufficient conditions which ensure the existence of the global attractors for semigroup. Then we recall the concept of measure of noncompactness of a set and recapitulate its basic properties. Finally, we prove that these two conditions are equivalent directly.

Sign-Changing Solutions for Discrete Dirichlet Boundary Value Problem  [PDF]
Yuhua Long, Baoling Zeng
Journal of Applied Mathematics and Physics (JAMP) , 2017, DOI: 10.4236/jamp.2017.511182
Abstract: Using invariant sets of descending flow and variational methods, we establish some sufficient conditions on the existence of sign-changing solutions, positive solutions and negative solutions for second-order nonlinear difference equations with Dirichlet boundary value problem. Some results in the literature are improved.
Anal HPV Infection in HIV-Positive Men Who Have Sex with Men from China
Lei Gao,Feng Zhou,Xiangwei Li,Yu Yang,Yuhua Ruan,Qi Jin
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0015256
Abstract: Anal HPV infection, which contributes to the development of anal warts and anal cancer, is well known to be common among men who have sex with men (MSM), especially among those HIV positives. However, HIV and anal HPV co-infection among MSM has not been addressed in China.
Integrating Overlapping Structures and Background Information of Words Significantly Improves Biological Sequence Comparison
Qi Dai, Lihua Li, Xiaoqing Liu, Yuhua Yao, Fukun Zhao, Michael Zhang
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0026779
Abstract: Word-based models have achieved promising results in sequence comparison. However, as the important statistical properties of words in biological sequence, how to use the overlapping structures and background information of the words to improve sequence comparison is still a problem. This paper proposed a new statistical method that integrates the overlapping structures and the background information of the words in biological sequences. To assess the effectiveness of this integration for sequence comparison, two sets of evaluation experiments were taken to test the proposed model. The first one, performed via receiver operating curve analysis, is the application of proposed method in discrimination between functionally related regulatory sequences and unrelated sequences, intron and exon. The second experiment is to evaluate the performance of the proposed method with f-measure for clustering Hepatitis E virus genotypes. It was demonstrated that the proposed method integrating the overlapping structures and the background information of words significantly improves biological sequence comparison and outperforms the existing models.
Computational Study on the Conformation and Vibration Frequencies of β-Sheet of ε-Polylysine in Vacuum
Shiru Jia,Zhiwen Mo,Yujie Dai,Xiuli Zhang,Hongjiang Yang,Yuhua Qi
International Journal of Molecular Sciences , 2009, DOI: 10.3390/ijms10083358
Abstract: Two oligomers, each containing 3 L-lysine residues, were used as model molecules for the simulation of the β-sheet conformation of ε-polylysine (ε-PLL) chains. Their C terminals were capped with ethylamine and N terminals were capped with α-L-aminobutanoic acid, respectively. The calculations were carried out with the hybrid two-level ONOIM (B3LYP/6-31G:PM3) computational chemistry method. The optimized conformation was obtained and IR frequencies were compared with experimental data. The result indicated that the two chains were winded around each other to form a distinct cyclohepta structure through bifurcated hydrogen bonds. The groups of amide and α-amidocyanogen coming from one chain and the carbonyl group from the other chain were involved in the cyclohepta structure. The bond angle of the bifurcated hydrogen bonds was 66.6°. The frequency analysis at ONIOM [B3LYP/6-31G (d):PM3] level showed the IR absorbances of the main groups, such as the amide and amidocyanogen groups, were in accordance with the experimental data.
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