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A novel method of measuring the positive-sequence capacitance
of T-connection transmission lines is proposed. The mathematical model of the
new method is explained in detail. In order to obtain enough independent
equations, three independent operation modes of T-connection transmission lines
during the line measurement are introduced. The digital simulation results and
field measurement results are shown. The simulation and measurement results
have validated that the new method can meet the needs of measuring the
positive-sequence capacitance of T-connection transmission lines. This method
has been implemented in the newly developed measurement instrument.
The broken efficiency of cell wall and the
release amount of Pichia pastoris intracellular protein under different cell
breaking conditions were investigated in this paper. The results showed that
broken efficiency using hot alkali combined with high-pressure homogenizing
method was higher than that of enzyme hydrolysis, hot alkali treatment and
high-pressure homogenation, respectively. Suspended medium had little effect on
the broken efficiency of yeast cell, but had significant effect on the protein
release yield. The results indicated that optimal condition for intracellular
proteins extraction was 30% (wet weight, w/v) of yeast cells suspend in 50 mM
phosphate buffer (pH 10.0), water bathed at 60?C for 2 hours, homogenized twice
at 100 MPa pressure. The broken efficiency of Pichia pastoris cell could reach
87.6% and the protein yield was 35.48 g per 100 g cells.
In this analysis, the single crystal of schiff base has been synthesized and the purity of material has been increased by repeated recrystallization process. Single crystal was grown by adopting the method growing in a slow evaporation solution using ethanol as solvent at room temperature. A new fluorescent molecule based on Schiff base has been synthesised and its binding properties investigated by fluorescence spectroscopy to show that it can selectively bind Cu2+ with fluorescence quenching.