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Search Results: 1 - 10 of 18514 matches for " Yuanji Han "
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Bifurcation of positive solutions for a semilinear equation with critical Sobolev exponent
Yuanji Cheng
Electronic Journal of Differential Equations , 2006,
Abstract: In this note we consider bifurcation of positive solutions to the semilinear elliptic boundary-value problem with critical Sobolev exponent $$displaylines{ -Delta u = lambda u - alpha u^p+ u^{2^*-1}, quad u >0 , quad hbox{in } Omega,cr u=0, quad hbox{on } partialOmega. }$$ where $Omega subset mathbb{R}^n$, $nge 3 $ is a bounded $C^2$-domain $lambda>lambda_1$, $1 0$ is a bifurcation parameter. Brezis and Nirenberg [2] showed that a lower order (non-negative) perturbation can contribute to regain the compactness and whence yields existence of solutions. We study the equation with an indefinite perturbation and prove a bifurcation result of two solutions for this equation.
The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants
Yuanji Zhang, Liangjiang Wang
BMC Evolutionary Biology , 2005, DOI: 10.1186/1471-2148-5-1
Abstract: We searched all publicly available sequence data for WRKY genes. A single copy of the WRKY gene encoding two WRKY domains was identified from Giardia lamblia, a primitive eukaryote, Dictyostelium discoideum, a slime mold closely related to the lineage of animals and fungi, and the green alga Chlamydomonas reinhardtii, an early branching of plants. This ancestral WRKY gene seems to have duplicated many times during the evolution of plants, resulting in a large family in evolutionarily advanced flowering plants. In rice, the WRKY gene family consists of over 100 members. Analyses suggest that the C-terminal domain of the two-WRKY-domain encoding gene appears to be the ancestor of the single-WRKY-domain encoding genes, and that the WRKY domains may be phylogenetically classified into five groups. We propose a model to explain the WRKY family's origin in eukaryotes and expansion in plants.WRKY genes seem to have originated in early eukaryotes and greatly expanded in plants. The elucidation of the evolution and duplicative expansion of the WRKY genes should provide valuable information on their functions.Transcriptional control is a major mechanism whereby a cell or organism regulates its gene expression. Sequence-specific DNA-binding transcription regulators, one class of transcription factors [1], play an essential role in modulating the rate of transcription of specific target genes. In this way, they direct the temporal and spatial expressions necessary for normal development and proper response to physiological or environmental stimuli. Comparative genome analysis reveals that genes for transcription regulators are abundantly present in plant and animal genomes, and the evolution and diversity of eukaryotes seem to be related to the expansion of lineage-specific transcription regulator families [2].WRKY proteins are recently identified transcriptional regulators comprising a large gene family [3]. The first cDNA encoding a WRKY protein, SPF1, was cloned from sweet p
Study on the Genetic Diversity of Osmanthus fragrans Cultivars
桂花品种资源的遗传多样性分析

Yuanji Han,Meifang Dong,Wangjun Yuan,Fude Shang,
韩远记
,董美芳,袁王俊,尚富德

植物学报 , 2008,
Abstract: We used morphological features and amplified fragment-length polymorphism (AFLP) markers to analyze the genetic diversity of 45 Osmanthus fragrans cultivars and found high levels of genetic diversity among the cultivars. Of the AFLP bands detected, 57.46% are polymorphic. We classified the cultivars biometrically according to their major morphological features and performed cluster analysis of the AFLP data by the unweighted pair-group method using arithmetic averages. The results of both approaches showed that O. fragrans cultivars of the Guilin region form a distinct subgroup, and flower color is aviable classification criterion. We discuss systems for O. fragrans cultivar classification.
Logistic approximations and their consequences to bifurcations patterns and long-run dynamics
Torsten Lindstr?m,Yuanji Cheng
Mathematics , 2014,
Abstract: On infinitesimally short time interval various processes contributing to population change tend to operate independently so that we can simply add their contributions (Metz and Diekmann (1986)). This is one of the cornerstones for differential equations modeling in general. Complicated models for processes interacting in a complex manner may be built up, and not only in population dynamics. The principle holds as long as the various contributions are taken into account exactly. In this paper we discuss commonly used approximations that may lead to dependency terms affecting the long run qualitative behavior of the involved equations. We prove that these terms do not produce such effects in the simplest and most interesting biological case, but the general case is left open.
Analysis of plant-derived miRNAs in animal small RNA datasets
Zhang Yuanji,Wiggins B,Lawrence Christina,Petrick Jay
BMC Genomics , 2012, DOI: 10.1186/1471-2164-13-381
Abstract: Background Plants contain significant quantities of small RNAs (sRNAs) derived from various sRNA biogenesis pathways. Many of these sRNAs play regulatory roles in plants. Previous analysis revealed that numerous sRNAs in corn, rice and soybean seeds have high sequence similarity to animal genes. However, exogenous RNA is considered to be unstable within the gastrointestinal tract of many animals, thus limiting potential for any adverse effects from consumption of dietary RNA. A recent paper reported that putative plant miRNAs were detected in animal plasma and serum, presumably acquired through ingestion, and may have a functional impact in the consuming organisms. Results To address the question of how common this phenomenon could be, we searched for plant miRNAs sequences in public sRNA datasets from various tissues of mammals, chicken and insects. Our analyses revealed that plant miRNAs were present in the animal sRNA datasets, and significantly miR168 was extremely over-represented. Furthermore, all or nearly all (>96%) miR168 sequences were monocot derived for most datasets, including datasets for two insects reared on dicot plants in their respective experiments. To investigate if plant-derived miRNAs, including miR168, could accumulate and move systemically in insects, we conducted insect feeding studies for three insects including corn rootworm, which has been shown to be responsive to plant-produced long double-stranded RNAs. Conclusions Our analyses suggest that the observed plant miRNAs in animal sRNA datasets can originate in the process of sequencing, and that accumulation of plant miRNAs via dietary exposure is not universal in animals.
Establishing an In Vivo Assay System to Identify Components Involved in Environmental RNA Interference in the Western Corn Rootworm
Keita Miyata, Parthasarathy Ramaseshadri, Yuanji Zhang, Gerrit Segers, Renata Bolognesi, Yoshinori Tomoyasu
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0101661
Abstract: The discovery of environmental RNA interference (RNAi), in which gene expression is suppressed via feeding with double-stranded RNA (dsRNA) molecules, opened the door to the practical application of RNAi-based techniques in crop pest management. The western corn rootworm (WCR, Diabrotica virgifera virgifera) is one of the most devastating corn pests in North America. Interestingly, WCR displays a robust environmental RNAi response, raising the possibility of applying an RNAi-based pest management strategy to this pest. Understanding the molecular mechanisms involved in the WCR environmental RNAi process will allow for determining the rate limiting steps involved with dsRNA toxicity and potential dsRNA resistance mechanisms in WCR. In this study, we have established a two-step in vivo assay system, which allows us to evaluate the involvement of genes in environmental RNAi in WCR. We show that laccase 2 and ebony, critical cuticle pigmentation/tanning genes, can be used as marker genes in our assay system, with ebony being a more stable marker to monitor RNAi activity. In addition, we optimized the dsRNA dose and length for the assay, and confirmed that this assay system is sensitive to detect well-known RNAi components such as Dicer-2 and Argonaute-2. We also evaluated two WCR sid1- like (sil) genes with this assay system. This system will be useful to quickly survey candidate systemic RNAi genes in WCR, and also will be adaptable for a genome-wide RNAi screening to give us an unbiased view of the environmental/systemic RNAi pathway in WCR.
Solving Large Scale Nonlinear Equations by a New ODE Numerical Integration Method  [PDF]
Tianmin Han, Yuhuan Han
Applied Mathematics (AM) , 2010, DOI: 10.4236/am.2010.13027
Abstract: In this paper a new ODE numerical integration method was successfully applied to solving nonlinear equations. The method is of same simplicity as fixed point iteration, but the efficiency has been significantly improved, so it is especially suitable for large scale systems. For Brown’s equations, an existing article reported that when the dimension of the equation N = 40, the subroutines they used could not give a solution, as compared with our method, we can easily solve this equation even when N = 100. Other two large equations have the dimension of N = 1000, all the existing available methods have great difficulties to handle them, however, our method proposed in this paper can deal with those tough equations without any difficulties. The sigularity and choosing initial values problems were also mentioned in this paper.
Solving Load Flow Problems of Power System by Explicit Pseudo-Transient Continuation (E-ψtc) Method  [PDF]
Tianmin Han, Yuhuan Han
Energy and Power Engineering (EPE) , 2016, DOI: 10.4236/epe.2016.82009
Abstract: This paper is a further study of two papers [1] and [2], which were related to Ill-Conditioned Load Flow Problems and were published by IEEE Trans. PAS. The authors of this paper have some different opinions, for example, the 11-bus system is not an ill-conditioned system. In addition, a new approach to solve Load Flow Problems, E-ψtc, is introduced. It is an explicit method; solving linear equations is not needed. It can handle very tough and very large systems. The advantage of this method has been fully proved by two examples. The authors give this new method a detailed description of how to use it to solve Load Flow Problems and successfully apply it to the 43-bus and the 11-bus systems. The authors also propose a strategy to test the reliability, and by solving gradient equations, this new method can answer if the solution exists or not.
Proteomic Analyses Reveal Common Promiscuous Patterns of Cell Surface Proteins on Human Embryonic Stem Cells and Sperms
Bin Gu,Jiarong Zhang,Ying Wu,Xinzong Zhang,Zhou Tan,Yuanji Lin,Xiao Huang,Liangbiao Chen,Kangshou Yao,Ming Zhang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019386
Abstract: It has long been proposed that early embryos and reproductive organs exhibit similar gene expression profiles. However, whether this similarity is propagated to the protein level remains largely unknown. We have previously characterised the promiscuous expression pattern of cell surface proteins on mouse embryonic stem (mES) cells. As cell surface proteins also play critical functions in human embryonic stem (hES) cells and germ cells, it is important to reveal whether a promiscuous pattern of cell surface proteins also exists for these cells.
Characterization of Unique Small RNA Populations from Rice Grain
Sara E. Heisel, Yuanji Zhang, Edwards Allen, Liang Guo, Tracey L. Reynolds, Xiao Yang, David Kovalic, James K. Roberts
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0002871
Abstract: Small RNAs (~20 to 24 nucleotides) function as naturally occurring molecules critical in developmental pathways in plants and animals [1], [2]. Here we analyze small RNA populations from mature rice grain and seedlings by pyrosequencing. Using a clustering algorithm to locate regions producing small RNAs, we classified hotspots of small RNA generation within the genome. Hotspots here are defined as 1 kb regions within which small RNAs are significantly overproduced relative to the rest of the genome. Hotspots were identified to facilitate characterization of different categories of small RNA regulatory elements. Included in the hotspots, we found known members of 23 miRNA families representing 92 genes, one trans acting siRNA (ta-siRNA) gene, novel siRNA-generating coding genes and phased siRNA generating genes. Interestingly, over 20% of the small RNA population in grain came from a single foldback structure, which generated eight phased 21-nt siRNAs. This is reminiscent of a newly arising miRNA derived from duplication of progenitor genes [3], [4]. Our results provide data identifying distinct populations of small RNAs, including phased small RNAs, in mature grain to facilitate characterization of small regulatory RNA expression in monocot species.
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