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Search Results: 1 - 10 of 101095 matches for " Yu-Jen Wu "
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McDonaldisation and the Labour Process: Impacts and Resistance
Yu-Jen Wu
Asian Social Science , 2009, DOI: 10.5539/ass.v5n5p103
Abstract: McDonaldisation is the process by which the principles of the fast-food restaurant are coming to dominate more and more sectors of society. The aims of this article are to explore the possible impacts of McDonaldisation on the labour process and expose the hidden agenda behind McDonaldisation. My main argument is that McDonaldisation is the realization of an extremely instrumental rationality which deliberately develops a variety of strategies to achieve the interests of capital. The article indicates that, despite the prevailing of McDonaldisation, there might be two forces that seems likely to drive the labour process away from the McDonaldised working rules: subjectivity and flexibility. The article is concluded that McDonaldisation is gradually blurring and even eroding the boundary between the customers and the workers. The customers used to be the source of profit-making but are now asked to a part of the labour force, that is an exploitation of the customers to supplement McDonaldisation in the exploitation of labours.
The Influence of the Distance from the Contact Point to the Crest of Bone on the Presence of the Interproximal Dental Papilla.
Yu-Jen Wu,Yu-Kang Tu,Shay-Min Huang,Chiu-Po Chan
Chang Gung Medical Journal , 2003,
Abstract: Background: Loss of the interproximal dental papilla may cause functional and, especiallyin the maxillary anterior region, phonetic and severe esthetic problems. Thepurpose of this study was to investigate whether the distance from the contactpoint to the bone crest on standardized periapical radiographs of themaxillary anterior teeth could be correlated with the presence of the interproximalpapilla in Taiwanese patients.Methods: In total, 200 interproximal sites of maxillary anterior teeth in 45 randomlyselected patients were examined. Selected subjects were adult Taiwanesewith fully erupted permanent dentition. The presence of the interproximalpapilla was determined visually. If there was no visible space apical to thecontact area, the papilla was recorded as being present. The distance from thecontact point to the crest of bone was measured on standardized periapicalradiographs using a paralleling technique with a RinnXCP holder.Results: Data revealed that when the distance from the contact point to the bone creston standardized periapical radiographs was 5 mm or less, the papillae werealmost 100% present. When the distance was 6 mm, 51% of the papillaewere present, and when the distance was 7 mm or greater, only 23% of thepapillae were present.Conclusion: The distance from the contact point to the bone crest on standardized periapicalradiographs of the maxillary anterior teeth is highly associated with thepresence or absence of the interproximal papilla in Taiwanese patients, and isa useful guide for clinical evaluation.
A New Spatane Diterpenoid from the Cultured Soft Coral Sinularia leptoclados
Tsung-Chang Tsai,Yu-Jen Wu,Jui-Hsin Su,Wei-Tung Lin,Yun-Sheng Lin
Marine Drugs , 2013, DOI: 10.3390/md11010114
Abstract: A new spatane diterpenoid, leptoclalin A ( 1), along with two previously reported known norcembranoid diterpenes ( 2 and 3), were isolated from a cultured soft coral Sinularia leptoclados . The structures were determined by extensive spectroscopic analyses and by comparison with the spectral data of related known compounds. Metabolite 1 is rarely found in spatane skeletons reported from soft corals. In addition, compound 1 exhibited weak cytotoxicity towards human tumor cell lines T-47 D and K-562.
Oxygenated Cembranoids from the Soft Coral Sinularia flexibilis
Ching-Chyuan Su,Bing-Sang Wong,Chuen Chin,Yu-Jen Wu,Jui-Hsin Su
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms14024317
Abstract: Chemical examination of the Taiwanese soft coral Sinularia flexibilis led to the isolation of five cembrane-based diterpenoids 1– 5, including two new metabolites, 11-acetylsinuflexolide ( 1) and 11-acetyldihydrosinuflexolide ( 2). The structures of the new metabolites were determined based on extensive spectroscopic analysis, particularly mass spectrometry and 2D NMR ( 1H– 1H COSY, HMQC, HMBC, and NOESY) spectroscopy. Metabolites 1, 3 and 4 exhibited moderate to weak cytotoxicity to human tumor cell lines, HeLa, HEp-2, MCF-7 and MDA-MB-231.
An Investigation into the Cytotoxic Effects of 13-Acetoxysarcocrassolide from the Soft Coral Sarcophyton crassocaule on Bladder Cancer Cells
Ching-Chyuan Su,Jui-Hsin Su,Jen-Jie Lin,Cheng-Chi Chen,Wen-Ing Hwang,Han Hsiang Huang,Yu-Jen Wu
Marine Drugs , 2011, DOI: 10.3390/md9122622
Abstract: Active compounds from natural products have been widely studied. The anti-tumor effects of 13-acetoxysarcocrassolide isolated from Formosan soft coral Sarcophyton crassocaule on bladder cancer cells were examined in this study. An MTT assay showed that 13-acetoxysarcocrassolide was cytotoxic to bladder female transitional cancer (BFTC) cells. We determined that the BFTC cells underwent cell death through apoptosis by flow cytometry. Due to the highly-migratory nature of the BFTC cells, the ability of 13-acetoxysarcocrassolide to stop their migration was assessed by a wound healing assay. To determine which proteins were affected in the BFTC cells upon treatment, a comparative proteomic analysis was performed. By LC-MS/MS analysis, we identified that 19 proteins were up-regulated and eight were down-regulated. Seven of the proteins were confirmed by western blotting analysis. This study reveals clues to the potential mechanism of the cytotoxic effects of 13-acetoxysarcocrassolide on BFTC cells. Moreover, it suggests that PPT1 and hnRNP F could be new biomarkers for bladder cancer. The results of this study are also helpful for the diagnosis, progression monitoring and therapeutic strategies of transitional cell tumors.
MCRS2 represses the transactivation activities of Nrf1
Jia-Long Wu, Young-Sun Lin, Chi-Chiang Yang, Yu-Jen Lin, Shan-Fu Wu, Ying-Ting Lin, Chien-Fu Huang
BMC Cell Biology , 2009, DOI: 10.1186/1471-2121-10-9
Abstract: To find other proteins interacting with the CNC bZIP domain of Nrf1, the CNC-bZIP region of Nrf1 was used as a bait in a yeast two-hybrid screening assay. MCRS2, a splicing variant of p78/MCRS1, was isolated as the Nrf1-interacting partner from the screenings. The interaction between Nrf1 and MCRS2 was confirmed in vitro by GST pull-down assays and in vivo by co-immunoprecipitation. Further, the Nrf1-MCRS2 interaction domains were mapped to the residues 354–447 of Nrf1 as well as the residues 314–475 of MCRS2 respectively, by yeast two-hybrid and GST pull-down assays. By immunofluorescence, MCRS2-FLAG was shown to colocalize with HA-Nrf1 in the nucleus and didn't result in the redistribution of Nrf1. This suggested the existence of Nrf1-MCRS2 complex in vivo. To further confirm the biological function, a reporter driven by CNC-bZIP protein binding sites was also shown to be repressed by MCRS2 in a transient transfection assay. An artificial reporter gene activated by LexA-Nrf1 was also specifically repressed by MCRS2.From the results, we showed MCRS2, a new Nrf1-interacting protein, has a repression effect on Nrf1-mediated transcriptional activation. This was the first ever identified repressor protein related to Nrf1 transactivation.Nrf1 (NF-E2 related factor1) belongs to the Cap'n'Collar-basic leucine zipper proteins (CNC-bZIP). The CNC-bZIP family is identified by its homology region, named the CNC domain, immediately N-terminal to the bZIP domain [1]. This family contains a basic domain interacting with sequence-specific DNA and a leucine zipper domain (bZIP) involved in protein-protein dimerization [2-4]. The members of Cap'n'Collar (CNC) family contain Nrf1, Nrf2, Nrf3, p45 NF-E2, Drosophila CNC protein, as well as C. elegans Skn-1 [5-9]. There are two known transcriptional roles of the CNC-bZIP family. First, they are involved in globin gene expression. Transcriptional control of the human beta-globin gene cluster is mediated by four DNase I hypersensitive si
Proteomic Analysis of Anti-Tumor Effects of 11-Dehydrosinulariolide on CAL-27 Cells
Chih-I Liu,Cheng-Chi Chen,Jiing-Chuan Chen,Jui-Hsin Su,Han Hsiang Huang,Jeff Yi-Fu Chen,Yu-Jen Wu
Marine Drugs , 2011, DOI: 10.3390/md9071254
Abstract: The anti-tumor effects of 11-dehydrosinulariolide, an active ingredient isolated from soft coral Sinularia leptoclados, on CAL-27 cells were investigated in this study. In the MTT assay for cell proliferation, increasing concentrations of 11-dehydrosinulariolide decreased CAL-27 cell viability. When a concentration of 1.5?μg/mL of 11-dehydrosinulariolide was applied, the CAL-27 cells viability was reduced to a level of 70% of the control sample. The wound healing function decreased as the concentration of 11-dehydrosinulariolide increased. The results in this study indicated that treatment with 11-dehydrosinulariolide for 6 h significantly induced both early and late apoptosis of CAL-27 cells, observed by flow cytometric measurement and microscopic fluorescent observation. A comparative proteomic analysis was conducted to investigate the effects of 11-dehydrosinulariolide on CAL-27 cells at the molecular level by comparison between the protein profiling (revealed on a 2-DE map) of CAL-27 cells treated with 11-dehydrosinulariolide and that of CAL-27 cells without the treatment. A total of 28 differential proteins (12 up-regulated and 16 down-regulated) in CAL-27 cells treated with 11-dehydrosinulariolide have been identified by LC-MS/MS analysis. Some of the differential proteins are associated with cell proliferation, apoptosis, protein synthesis, protein folding, and energy metabolism. The results of this study provided clues for the investigation of biochemical mechanisms of the anti-tumor effects of 11-dehydrosinulariolide on CAL-27 cells and could be valuable information for drug development and progression monitoring of oral squamous cell carcinoma (OSCC).
Amniotic Fluid Stem Cells with Low γ-Interferon Response Showed Behavioral Improvement in Parkinsonism Rat Model
Yu-Jen Chang, Tsung-Yen Ho, Mei-Ling Wu, Shiaw-Min Hwang, Tzyy-Wen Chiou, Ming-Song Tsai
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0076118
Abstract: Amniotic fluid stem cells (AFSCs) are multipotent stem cells that may be used in transplantation medicine. In this study, AFSCs established from amniocentesis were characterized on the basis of surface marker expression and differentiation potential. To further investigate the properties of AFSCs for translational applications, we examined the cell surface expression of human leukocyte antigens (HLA) of these cells and estimated the therapeutic effect of AFSCs in parkinsonian rats. The expression profiles of HLA-II and transcription factors were compared between AFSCs and bone marrow-derived mesenchymal stem cells (BMMSCs) following treatment with γ-IFN. We found that stimulation of AFSCs with γ-IFN prompted only a slight increase in the expression of HLA-Ia and HLA-E, and the rare HLA-II expression could also be observed in most AFSCs samples. Consequently, the expression of CIITA and RFX5 was weakly induced by γ-IFN stimulation of AFSCs compared to that of BMMSCs. In the transplantation test, Sprague Dawley rats with 6-hydroxydopamine lesioning of the substantia nigra were used as a parkinsonian-animal model. Following the negative γ-IFN response AFSCs injection, apomorphine-induced rotation was reduced by 75% in AFSCs engrafted parkinsonian rats but was increased by 53% in the control group after 12-weeks post-transplantation. The implanted AFSCs were viable, and were able to migrate into the brain’s circuitry and express specific proteins of dopamine neurons, such as tyrosine hydroxylase and dopamine transporter. In conclusion, the relative insensitivity AFSCs to γ-IFN implies that AFSCs might have immune-tolerance in γ-IFN inflammatory conditions. Furthermore, the effective improvement of AFSCs transplantation for apomorphine-induced rotation paves the way for the clinical application in parkinsonian therapy.
Inhibition of Melanogenesis by Gallic Acid: Possible Involvement of the PI3K/Akt, MEK/ERK and Wnt/β-Catenin Signaling Pathways in B16F10 Cells
Tzu-Rong Su,Jen-Jie Lin,Chi-Chu Tsai,Tsu-Kei Huang,Zih-Yan Yang,Ming-O Wu,Yu-Qing Zheng,Ching-Chyuan Su,Yu-Jen Wu
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms141020443
Abstract: Gallic acid is one of the major flavonoids found in plants. It acts as an antioxidant, and seems to have anti-inflammatory, anti-viral, and anti-cancer properties. In this study, we investigated the effects of gallic acid on melanogenesis, including the activation of melanogenesis signaling pathways. Gallic acid significantly inhibited both melanin synthesis and tyrosinase activity in a dose- and time-dependent manner, and decreased the expression of melanogenesis-related proteins, such as microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP1), and dopachrome tautomerase (Dct). In addition, gallic acid also acts by phosphorylating and activating melanogenesis inhibitory proteins such as Akt and mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK). Using inhibitors against PI3K/Akt (LY294002) or MEK/ERK-specific (PD98059), the hypopigmentation effect was suppressed, and the gallic acid-initiated activation of MEK/ERK and PI3K/Akt was also revoked. Gallic acid also increased GSK3β and p-β-catenin expression but down-regulated p-GSK3β. Moreover, GSK3β-specific inhibitor (SB216763) restored gallic acid-induced melanin reduction. These results suggest that activation of the MEK/ERK, PI3K/Akt, and inhibition of Wnt/β-catenin signaling pathways is involved in the melanogenesis signaling cascade, and that activation by gallic acid reduces melanin synthesis via down-regulation of MITF and its downstream signaling pathway. In conclusion, gallic acid may be a potentially agent for the treatment of certain skin conditions.
Induction of Apoptosis by 11-Dehydrosinulariolide via Mitochondrial Dysregulation and ER Stress Pathways in Human Melanoma Cells
Tzu-Rong Su,Feng-Jen Tsai,Jen-Jie Lin,Han Hsiang Huang,Chien-Chih Chiu,Jui-Hsin Su,Ya-Ting Yang,Jeff Yi-Fu Chen,Bing-Sang Wong,Yu-Jen Wu
Marine Drugs , 2012, DOI: 10.3390/md10081883
Abstract: In this study the isolated compound 11-dehydrosinulariolide from soft coral Sinularia leptoclados possessed anti-proliferative, anti-migratory and apoptosis-inducing activities against A2058 melanoma cells. Anti-tumor effects of 11-dehydrosinulariolide were determined by MTT assay, cell migration assay and flow cytometry. Growth and migration of melanoma cells were dose-dependently inhibited by 2–8 μg/mL 11-dehydrosinulariolide. Flow cytometric data indicated that 11-dehydrosinulariolide induces both early and late apoptosis in melanoma cells. It was found that the apoptosis induced by 11-dehydrosinulariolide is relevant to mitochondrial-mediated apoptosis via caspase-dependent pathways, elucidated by loss of mitochondrial membrane potential (?Ym), release of cytochrome C, activation of caspase-3/-9 and Bax as well as suppression of Bcl-2/Bcl-xL. The cleavage of PARP-1 suggested partial involvement of caspase-independent pathways. Immunoblotting data displayed up-regulations of PERK/eIF2α/ATF4/CHOP and ATF6/CHOP coupling with elevation of ER stress chaperones GRP78, GRP94, calnexin, calreticulin and PDI, implicating the involvement of these factors in ER stress-mediated apoptosis induced by 11-dehydrosinulariolide. The abolishment of apoptotic events after pre-treatment with salubrinal indicated that ER stress-mediated apoptosis is also induced by 11-dehydrosinulariolide against melanoma cells. The data in this study suggest that 11-dehydrosinulariolide potentially induces apoptosis against melanoma cells via mitochondrial dysregulation and ER stress pathways.
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