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Calidad de las semillas de accesiones de leguminosas colectadas en la región oriental de Cuba (Nota técnica)
González,Yolanda; Reino,J; Toral,Odalys;
Pastos y Forrajes , 2011,
Abstract: a collection mission was conducted during 2006 in the eastern region of cuba, which comprised zones of the provinces granma, guantánamo, holguín, bayamo, las tunas and camagüey. seeds from 95 legume accessions, 68 herbaceous ones and 27 trees, were collected. afterwards, a germination test was conducted, to know their quality (with seed coat cut). for that purpose, petri dishes were used on river sand. the number of seeds used was variable in each accession (according to the collected amount) and only one replication was used (without statistical design). the germination counts were made according to international rules. the best quality seeds corresponded to the tree species, although low germination was shown by cajanus cajan, cassia siamea, peltophorum ferrugianum and leucaena leucocephala, from the floro-pérez zone in the holguín province. they all showed 100% survival. seed quality depended on the time the seeds stayed in the field after the pod maturation stage. through this collection mission the germplasm of the eepf ?indio hatuey? increased with 64 new accessions. to continue collecting germplasm in other zones of the country is recommended, with emphasis on trees, which would allow achieving higher diversity from their use in livestock production systems, as well as to conduct the corresponding quality tests.
Dormancia y tratamientos pregerminativos en las semillas de Leucaena spp. cosechadas en suelo ácido
González,Yolanda; Reino,J; Machado,R;
Pastos y Forrajes , 2009,
Abstract: a trial was conducted with seeds of leucaena spp accessions harvested on acid soil and stored under ambient conditions, in order to determine the period and intensity of dormancy and its breaking. for that purpose a simple classification design and four replications were used, with the following treatments: 1) untreated seed (control); 2) coat cut; and 3) water at 80oc for 2 minutes. in each one of the accessions there were significant differences among the applied treatments and the highest germination values were found with the seed coat cut. all the accessions showed dormant state (between 10 and 98%), which was observed in the germination of the control and the viability with the seed coat cut. the highest dormancy values in the seeds harvested in 2006 were detected in l. macrophylla ciat 17233, l. macrophylla ciat 17231, l. diversifolia ciat 17270 and l. leucocephala ih-1140, which hardness was 98,0; 97,0; 80,0 and 74,7%, respectively; in the seeds harvested in 2003 and 2004, the hardness percentages were 89,0; 69,0 and 60,0% for l. lanceolata ciat 17252, l. macrophylla ciat 17238 and l. leucocephala cv. k-67, respectively. the seeds of the accessions were concluded to show postharvest dormancy, which varied between 10 and 98%. in addition, thermal scarification caused dormancy breaking, for which the application of this procedure before planting to leucaena spp seeds harvested on acid soil in cascajal, is recommended.
Calidad de las semillas de accesiones colectadas en las regiones occidental, oriental y central de Cuba (Nota técnica)
González,Yolanda; Reino,J; Toral,Odalys;
Pastos y Forrajes , 2011,
Abstract: two collection missions were conducted during 2007 (western and eastern regions) and 2009 (central region) in cuba, which comprised certain zones of the provinces granma, guantánamo, holguín, las tunas, camagüey, pinar del rio, matanzas, sancti spiritus and villa clara. seeds from 88 accessions (52 tree and 36 herbaceous accessions) were collected. afterwards, all of them went through a germination test in petri dishes with river sand, with a previous coat cut, to know their quality. the number of used seeds was variable in each accession, in correspondence with the sample size and only one replication was used. the germination counts were made according to international rules. high quality was obtained in the seeds of tree and shrub species (70-100%), which did not happen in swietenia sp. from granma (0 and 8%) or in jatropha curcas collected in pinar del río, guantánamo (0%) and banao (8%), or in albizia lebbeck from guantánamo or guazuma ulmifolia (24%) collected in villa clara. for the herbaceous legumes germination varied between 80 and 100%, except in macroptilium atropurpureum (60%) and in teramnus (12%) from granma, in neonotonia wightii (20%) collected in matanzas, in calopogonium sp. (52%) from banao and in glycine sp. (50%) from sancti spiritus. seed quality was concluded to depend on the time they were in the field after pod maturation. to continue the collection in other zones of the country is recommended, which will allow higher diversity in livestock production systems.
Dormancia y tratamientos pregerminativos en las semillas de Leucaena spp. cosechadas en suelo ácido Dormancy and pregerminative treatments in Leucaena spp. seeds harvested on acid soil
Yolanda González,J Reino,R Machado
Pastos y Forrajes , 2009,
Abstract: Se desarrolló un experimento con semillas de accesiones de Leucaena spp. cosechadas en suelo ácido y almacenadas bajo condiciones ambientales, para determinar el período e intensidad de la dormancia y su ruptura. Para ello se empleó un dise o de clasificación simple y cuatro réplicas, con los siguientes tratamientos: 1) semilla sin tratar (control); 2) corte de cubierta; y 3) agua a 80°C durante 2 minutos. En cada una de las accesiones hubo diferencias significativas entre los tratamientos aplicados y los mayores valores de germinación se encontraron con el corte de cubierta. Todas las accesiones mostraron estado dormático (entre 10 y 98%), el cual se apreció en la germinación del control y en la viabilidad con el corte de cubierta. Los mayores valores de dormancia en las semillas cosechadas en el 2006 se detectaron en L. macrophylla CIAT 17233, L. macrophylla CIAT 17231, L. diversifolia CIAT 17270 y L. leucocephala IH-1140, cuya dureza fue de 98,0; 97,0; 80,0 y 74,7%, respectivamente; en las cosechadas en el 2003 y 2004, los porcentajes de dureza fueron de 89,0; 69,0 y 60,0% para L. lanceolata CIAT 17252, L. macrophylla CIAT 17238 y L. leucocephala cv. K-67, respectivamente. Se concluye que las semillas de las accesiones presentaron dormancia poscosecha, que varió entre 10 y 98%. Además la escarificación térmica provocó su ruptura, por lo que se recomienda aplicar este procedimiento antes de la siembra a las semillas de Leucaena spp. que se cosechen en suelo ácido de Cascajal. A trial was conducted with seeds of Leucaena spp accessions harvested on acid soil and stored under ambient conditions, in order to determine the period and intensity of dormancy and its breaking. For that purpose a simple classification design and four replications were used, with the following treatments: 1) untreated seed (control); 2) coat cut; and 3) water at 80oC for 2 minutes. In each one of the accessions there were significant differences among the applied treatments and the highest germination values were found with the seed coat cut. All the accessions showed dormant state (between 10 and 98%), which was observed in the germination of the control and the viability with the seed coat cut. The highest dormancy values in the seeds harvested in 2006 were detected in L. macrophylla CIAT 17233, L. macrophylla CIAT 17231, L. diversifolia CIAT 17270 and L. leucocephala IH-1140, which hardness was 98,0; 97,0; 80,0 and 74,7%, respectively; in the seeds harvested in 2003 and 2004, the hardness percentages were 89,0; 69,0 and 60,0% for L. lanceolata CIAT 17252, L. macrophylla CIAT
Calidad de las semillas de accesiones de leguminosas colectadas en la región oriental de Cuba (Nota técnica) Seed quality of legume accessions collected in the eastern region of Cuba (Technical note)
Yolanda González,J Reino,Odalys Toral
Pastos y Forrajes , 2011,
Abstract: Se efectuó una misión de colecta durante el 2006 en la región oriental de Cuba, que comprendió zonas de las provincias de Granma, Guantánamo, Holguín, Bayamo, Las Tunas y Camagüey. Se colectaron semillas de 95 accesiones de leguminosas, de ellas 68 herbáceas y 27 arbóreas. Posteriormente se hizo una prueba de germinación, para conocer su calidad (con corte de cubierta). Para ello se utilizaron placas de Petri sobre arena de río. El número de semillas utilizadas fue variable en cada accesión (según la cantidad colectada) y solo se empleó una réplica (sin dise o estadístico). Los conteos de germinación se realizaron según las normas internacionales. Las semillas de mejor calidad correspondieron a las especies arbóreas, aunque presentaron baja germinación las de Cajanus cajan, Cassia siamea, Peltophorum ferrugianum y Leucaena leucocephala, procedentes de la zona de Floro-Pérez en la provincia de Holguín. Todas mostraron 100% de sobrevivencia. La calidad de las semillas dependió del tiempo que estuvieron en el campo después de la etapa de maduración de las legumbres. A través de esta misión de colecta se incrementó el germoplasma de la EEPF Indio Hatuey con 64 nuevas accesiones. Se recomienda la continuidad de la colecta de germoplasma en otras zonas del país, con énfasis en las plantas arbóreas, lo que permitirá lograr una mayor diversidad a partir de su uso en los sistemas de producción agropecuaria, así como llevar a cabo las pruebas de calidad correspondientes. A collection mission was conducted during 2006 in the eastern region of Cuba, which comprised zones of the provinces Granma, Guantánamo, Holguín, Bayamo, Las Tunas and Camagüey. Seeds from 95 legume accessions, 68 herbaceous ones and 27 trees, were collected. Afterwards, a germination test was conducted, to know their quality (with seed coat cut). For that purpose, Petri dishes were used on river sand. The number of seeds used was variable in each accession (according to the collected amount) and only one replication was used (without statistical design). The germination counts were made according to international rules. The best quality seeds corresponded to the tree species, although low germination was shown by Cajanus cajan, Cassia siamea, Peltophorum ferrugianum and Leucaena leucocephala, from the Floro-Pérez zone in the Holguín province. They all showed 100% survival. Seed quality depended on the time the seeds stayed in the field after the pod maturation stage. Through this collection mission the germplasm of the EEPF Indio Hatuey increased with 64 new accessions. To continue collectin
Calidad de las semillas de accesiones colectadas en las regiones occidental, oriental y central de Cuba (Nota técnica) Seed quality of accessions collected in the western, eastern and central regions of Cuba (Technical note)
Yolanda González,J Reino,Odalys Toral
Pastos y Forrajes , 2011,
Abstract: Se efectuaron dos misiones de colecta durante el 2007 (regiones occidental y oriental) y el 2009 (región central) en Cuba, que comprendió zonas puntuales de las provincias de Granma, Guantánamo, Holguín, Bayamo, Las Tunas, Camagüey, Pinar del Río, Matanzas, Sancti Spiritus y Villa Clara. Se colectaron semillas de 88 accesiones de leguminosas (52 arbóreas y 36 herbáceas). Posteriormente a todas se les realizó una prueba de germinación en placas de Petri con arena de río, previo corte de la cubierta, para conocer su calidad. El número de semillas utilizadas fue variable en cada accesión, en correspondencia con el tama o de la muestra, y solo se empleó una réplica. Los conteos de germinación se realizaron según las normas internacionales. Se obtuvo una alta calidad en las semillas de las especies arbóreas y arbustivas (70-100%), lo cual no ocurrió en las caobas (Swietenia sp.) de Granma (0 y 8%) ni en Jatropha curcas colectada en Pinar del Río, Guantánamo (0%) y Banao (8%), tampoco en Albizia lebbeck procedente de Guantánamo y en Guazuma ulmifolia (24%) de Villa Clara. Para las leguminosas herbáceas la germinación varió entre 80 y 100%, excepto en Macroptilium atropurpureum (60%) y en Teramnus (12%) procedentes de Granma, en Neonotonia wightii (20%) de Matanzas, en Calopogonium sp. (52%) de Banao y en Glycine sp. (50%) de Sancti Spíritus. Se concluye que la calidad de las semillas estuvo en dependencia del tiempo que estuvieron en el campo después de la maduración de las legumbres. Se recomienda la continuidad de la colecta en otras zonas del país, lo que permitirá una mayor diversidad en los sistemas de producción agropecuarios. Two collection missions were conducted during 2007 (western and eastern regions) and 2009 (central region) in Cuba, which comprised certain zones of the provinces Granma, Guantánamo, Holguín, Las Tunas, Camagüey, Pinar del Rio, Matanzas, Sancti Spiritus and Villa Clara. Seeds from 88 accessions (52 tree and 36 herbaceous accessions) were collected. Afterwards, all of them went through a germination test in Petri dishes with river sand, with a previous coat cut, to know their quality. The number of used seeds was variable in each accession, in correspondence with the sample size and only one replication was used. The germination counts were made according to international rules. High quality was obtained in the seeds of tree and shrub species (70-100%), which did not happen in Swietenia sp. from Granma (0 and 8%) or in Jatropha curcas collected in Pinar del Río, Guantánamo (0%) and Banao (8%), or in Albizia lebbeck from Guantánamo
Temperatura óptima de germinación y patrones de imbibición de las semillas de Albizia lebbeck, Gliricidia sepium y Bauhinia purpurea
Reino,J; González,Yolanda; Sánchez,J. A;
Pastos y Forrajes , 2008,
Abstract: fresh and aged seeds of the trees albizia lebbeck, gliricidia sepium and bauhinia purpurea from the experi-mental station of pastures and forages ?indio hatuey?, matanzas, cuba, were used. a simple classification experiment and five replications were designed for the germinative response to different temperatures of the substratum (constant 25oc and three alternate ones: 25/30oc, 25/35oc and 25/40oc). before seeding the seeds of a. lebbeck were scarified (water at 80oc/2'). for the imbibition pattern the seeds were put on filter paper moist with purified water according to the optimum germination temperature, under white fluorescent light and at different imbibition times. in the fresh seeds of all the species the highest percentages of final germination were obtained at alternate temperature of 25/35oc and in the aged ones at 25/30oc. with regards to the imbibition pattern they all followed a three-phase pattern of water absorption. the fresh and aged seeds of g. sepium showed a very similar imbibition pattern, however the fresh seeds of the other species presented higher hydration rate than the aged ones and consequently they reached faster the start of phase iii of the imbibition pattern (i.e. visible germination). the range of optimum temperature for the germination of aged seeds was concluded to be 25/30 oc and for the fresh ones it was 25/35 oc and both showed a three-phase pattern of water absorption when the seeding was carried out at optimum germination temperature.
Calidad de las accesiones colectadas en la región centro-oriental de Cuba (Nota técnica)
Reino,J; González,Yolanda; Toral,Odalys; Iglesias,J. M;
Pastos y Forrajes , 2010,
Abstract: a prospection was made in 2005, in the central-eastern region of cuba and seeds from 43 accessions (36 tree legumes and seven herbaceous ones) were collected. afterwards, a germination test was conducted in order to learn the quality of the seeds (with seed coat cut), in petri dishes on river sand. the number of seeds used in each accession was different (according to the collected ones) and only one replication was used (without statistical design). the best-quality seeds corresponded to the accessions bauhinia acuminata, bauhinia purpurea (from nuevitas and vertientes), cassia fistula, albizia kalkora, centrosema sp. and centrosema brasilianum (100% germination), as well as calopogonium caeruleum (90%) and albizia lucida (91,6%). seven accessions did not germinate. most of them achieved high germination (in bags), although in such species as bauhinia hookeli, b. acuminata, a. lebbeck, c. caeruleum, c. brasilianum, desmodium sp., and teramnus sp. it was low and two accessions did not survive. the germplasm of the eepf ?indio hatuey? increased in 34 accessions. the importance of knowing the seed quality and the relation between this indicator and their deterioration was proven, due to the large variability shown by the germination percentage in seeds collected in the same time period.
Calidad de las accesiones colectadas en la región centro-oriental de Cuba (Nota técnica) Quality of accessions collected in the central-eastern region of Cuba (Technical note)
J Reino,Yolanda González,Odalys Toral,J. M Iglesias
Pastos y Forrajes , 2010,
Abstract: Se efectuó una prospección en el 2005, en la región centro-oriental de Cuba, y se colectaron semillas de 43 accesiones (36 de leguminosas arbóreas y siete de herbáceas). Posteriormente se realizó una prueba de germinación para conocer la calidad de las semillas (con corte de cubierta), en placas de petri sobre arena de río. El número de semillas utilizadas en cada accesión fue diferente (según las colectadas) y solo se empleó una réplica (sin dise o estadístico). Las semillas de mejor calidad correspondieron a las accesiones Bauhinia acuminata, Bauhinia purpurea (de Nuevitas y Vertientes), Cassia fistula, Albizia kalkora, Centrosema sp. y Centrosema brasilianum (100% de germinación), así como Calopogonium caeruleum (90%) y Albizia lucida (91,6%). Siete accesiones no germinaron. La mayoría logró una alta sobrevivencia (en bolsas), aunque en especies como Bauhinia hookeli, B. acuminata, Albizia lebbeck, C. caeruleum, C. brasilianum, Desmodium sp. y Teramnus sp. fue baja y dos accesiones no sobrevivieron. El germoplasma de la EEPF Indio Hatuey se incrementó en 34 accesiones. Se demostró la importancia de conocer la calidad de las semillas y la relación entre este indicador y su deterioro, debido a la gran variabilidad que mostró el porcentaje de germinación en semillas colectadas en un mismo período de tiempo. A prospection was made in 2005, in the central-eastern region of Cuba and seeds from 43 accessions (36 tree legumes and seven herbaceous ones) were collected. Afterwards, a germination test was conducted in order to learn the quality of the seeds (with seed coat cut), in Petri dishes on river sand. The number of seeds used in each accession was different (according to the collected ones) and only one replication was used (without statistical design). The best-quality seeds corresponded to the accessions Bauhinia acuminata, Bauhinia purpurea (from Nuevitas and Vertientes), Cassia fistula, Albizia kalkora, Centrosema sp. and Centrosema brasilianum (100% germination), as well as Calopogonium caeruleum (90%) and Albizia lucida (91,6%). Seven accessions did not germinate. Most of them achieved high germination (in bags), although in such species as Bauhinia hookeli, B. acuminata, A. lebbeck, C. caeruleum, C. brasilianum, Desmodium sp., and Teramnus sp. it was low and two accessions did not survive. The germplasm of the EEPF Indio Hatuey increased in 34 accessions. The importance of knowing the seed quality and the relation between this indicator and their deterioration was proven, due to the large variability shown by the germination percentage in see
Temperatura óptima de germinación y patrones de imbibición de las semillas de Albizia lebbeck, Gliricidia sepium y Bauhinia purpurea Optimum germination temperature and imbibition patterns of the seeds from Albizia lebbeck, Gliricidia sepium and Bauhinia purpurea
J Reino,Yolanda González,J. A Sánchez
Pastos y Forrajes , 2008,
Abstract: Se utilizaron semillas frescas y envejecidas de las plantas arbóreas Albizia lebbeck, Gliricidia sepium y Bauhinia purpurea, procedentes de la Estación Experimental de Pastos y Forrajes Indio Hatuey , Matanzas, Cuba. Se dise ó un experimento de clasificación simple y cinco réplicas para la respuesta germinativa a diferentes temperaturas del sustrato (constante a 25oC y tres alternas: 25/30oC, 25/35oC y 25/40oC). Antes de la siembra las semillas de A. lebbeck se escarificaron (agua a 80oC/2'). Para el patrón de imbibición las semillas se colocaron sobre papel de filtro humedecido con agua destilada, según la temperatura óptima de germinación, en luz blanca fluorescente y en diferentes tiempos de imbibición. En las semillas frescas de todas las especies los mayores porcentajes de germinación final se obtuvieron a temperatura alterna de 25/35oC y en las enveje-cidas a 25/30oC. Con respecto al patrón de imbibición, todas siguieron un patrón trifásico de absorción de agua. Las semillas frescas y envejecidas de G.. sepium presentaron un patrón de imbibición muy similar; sin embargo, las semillas frescas de las otras especies tuvieron una mayor velocidad de hidratación que las enve-jecidas y, por consiguiente, alcanzaron el comienzo de la fase III del patrón de imbibición (i.e., germinación visible) más rápidamente. Se concluye que el rango de temperatura óptima para la germinación de las semillas envejecidas fue de 25/30oC y para las frescas fue de 25/35oC y ambas presentaron un patrón trifásico de absorción de agua cuando la siembra se realizó en la temperatura óptima de germinación. Fresh and aged seeds of the trees Albizia lebbeck, Gliricidia sepium and Bauhinia purpurea from the Experi-mental Station of Pastures and Forages Indio Hatuey , Matanzas, Cuba, were used. A simple classification experiment and five replications were designed for the germinative response to different temperatures of the substratum (constant 25oC and three alternate ones: 25/30oC, 25/35oC and 25/40oC). Before seeding the seeds of A. lebbeck were scarified (water at 80oC/2'). For the imbibition pattern the seeds were put on filter paper moist with purified water according to the optimum germination temperature, under white fluorescent light and at different imbibition times. In the fresh seeds of all the species the highest percentages of final germination were obtained at alternate temperature of 25/35oC and in the aged ones at 25/30oC. With regards to the imbibition pattern they all followed a three-phase pattern of water absorption. The fresh and aged seeds of G. sepium showed
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