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Search Results: 1 - 10 of 36 matches for " Yohtaroh Takagaki "
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Cell Shape and Cardiosphere Differentiation: A Revelation by Proteomic Profiling
Nanako Kawaguchi,Mitsuyo Machida,Kota Hatta,Toshio Nakanishi,Yohtaroh Takagaki
Biochemistry Research International , 2013, DOI: 10.1155/2013/730874
Abstract: Stem cells (embryonic stem cells, somatic stem cells such as neural stem cells, and cardiac stem cells) and cancer cells are known to aggregate and form spheroid structures. This behavior is common in undifferentiated cells and may be necessary for adapting to certain conditions such as low-oxygen levels or to maintain undifferentiated status in microenvironments including stem cell niches. In order to decipher the meaning of this spheroid structure, we established a cardiosphere clone (CSC-21E) derived from the rat heart which can switch its morphology between spheroid and nonspheroid. Two forms, floating cardiospheres and dish-attached flat cells, could be switched reversibly by changing the cell culture condition. We performed differential proteome analysis studies and obtained protein profiles distinct between spherical forms and flat cells. From protein profiling analysis, we found upregulation of glycolytic enzymes in spheroids with some stress proteins switched in expression levels between these two forms. Evidence has been accumulating that certain chaperone/stress proteins are upregulated in concert with cellular changes including proliferation and differentiation. We would like to discuss the possible mechanism of how these aggregates affect cell differentiation and/or other cellular functions. 1. Introduction Two epoch accomplishments in the first decade of 21st century are changing the scope of biomedical research. The first was the completion of the human genome project [1], which enabled the onset of “Omics” or the integrative approach (System Biology) [2]. The second was the discovery of adult stem cells in human [3] followed by induction of pluripotency by Yamanaka factors (Oct3/4, Sox, Klf4, and c-Myc) in both mouse and human somatic cells [4, 5]. Adult stem cells are undifferentiated cells found throughout the body after development. They have the ability to self-renew indefinitely and have the developmental potential to generate many other cell types due to cell fate switching induced by extracellular environmental signals [3]. Plasticity of stem cells as well as the induction and reprogramming of somatic cells ignited the hope of discovering cellular therapy for the regeneration of damaged body parts. The revelation of the involvement of extracellular factors in switching cell types resulted in paradigm shift from “genetic determinism”, the paradigm that all biological processes follow the one-way instruction stored in genomes to an “environment-genome interaction” understanding. Studies on the regulatory molecular mechanisms
Le discours direct avec guillemets sans verbe introducteur dans les textes traduits du japonais
Takagaki Yumi
SHS Web of Conferences , 2012, DOI: 10.1051/shsconf/20120100154
Abstract: Le présent article traite des phénomènes concernant le discours direct avec guillemets sans verbe introducteur. La spécificité de notre étude tient dans nos exemples extraits de textes traduits du japonais. Le choix de ce corpus n’est pas arbitraire. Notre objectif n’est pas d’entrer dans la linguistique contrastive des deux langues, mais de mettre en évidence des cas périphériques du fran ais. Car, le japonais dispose des formes du discours rapporté qui sont peu exploitées en fran ais, dont celle de discours direct avec guillemets sans verbe introducteur. Dans la traduction, la volonté d’être fidèle aux originaux donne un fran ais moins naturel, qui permettra pourtant de dégager des usages peu disponibles dans les exemples attestés. C’est dans cette perspective que nous étudions la forme de discours direct mis entre guillemets sans verbe introducteur. Avec des textes littéraires traduits du japonais, nous mettons en évidence les conditions qui favorisent l’apparition de cette forme. L’observation attentive des données montre que cette forme n’est possible que lorsqu’il y a une continuité thématique entre les paroles citées et le cotexte amont. Or dans les textes japonais, elle peut appara tre plus librement : la présence d’une expression de perception justifie son introduction. Cette restriction constitue une caractéristique de la logique de la textualisation fran aise.
Computational Modeling of Induction Hardening Process of Machine Parts
Yutaka Toi,Masakazu Takagaki
Lecture Notes in Engineering and Computer Science , 2008,
Avan os no tratamento do cancer pulmonar Advances in the treatment of lung cancer
Teresa Yae Takagaki
Jornal Brasileiro de Pneumologia , 2005, DOI: 10.1590/s1806-37132005000600002
Combinatorial expressions of the solutions to initial value problems of the discrete and ultradiscrete Toda molecules
Shuhei Kamioka,Tomoaki Takagaki
Physics , 2013, DOI: 10.1088/1751-8113/46/35/355203
Abstract: Combinatorial expressions are presented to the solutions to initial value problems of the discrete and ultradiscrete Toda molecules. For the discrete Toda molecule, a subtraction-free expression of the solution is derived in terms of non-intersecting paths, for which two results in combinatorics, Flajolet's interpretation of continued fractions and Gessel--Viennot's lemma on determinants, are applied. By ultradiscretizing the subtraction-free expression, the solution to the ultradiscrete Toda molecule is obtained. It is finally shown that the initial value problem of the ultradiscrete Toda molecule is exactly solved in terms of shortest paths on a specific graph.
Momentum transfer and flow induction in a dielectric barrier discharge plasma actuator
T. Abe,M. Takagaki,K. Yamada
AIP Advances , 2012, DOI: 10.1063/1.4768802
Abstract: The flow induction process resulting from a dielectric barrier discharge plasma actuator was experimentally investigated by a flow visualization method. This method enables a detailed examination of the flow induction process. The overall behavior as well as the detailed temporal and spatial behavior of the flow induction process was clarified. The induced flow originated from the vicinity of an exposed electrode edge, and similar to a wall jet, it smoothly spread over the surface until a vortex was generated at the tip of the wall jet. Even after the vortex disappeared, the wall jet continued to extend. This wall jet was classified into two distinct regions. The first region contained the wall jet extending from the electrode edge to the location where the vortex was generated. In this region, active momentum coupling occurred; therefore, the flow was actively induced. During active momentum transfer in the acceleration region, the periodic inflation of the wall jet thickness was synchronized with the decreasing phase of the applied high-voltage input, which suggested different mechanisms of momentum transfer during the increasing and decreasing phases of the high-voltage input. The second region was an extension of the first one and was formed through the inertia of the flow originating from the first region.
Rice Green Revolution and Climatic Change in East Africa: An Approach from the Technical Efficiency of Rainfed Rice Farmers in Uganda  [PDF]
Atsushi Maruyama, Yusuke Haneishi, Stella E. Okello, Godfrey Asea, Tatsushi Tsuboi, Michiko Takagaki, Masao Kikuchi
Agricultural Sciences (AS) , 2014, DOI: 10.4236/as.2014.54035

In East Africa where a drastic improvement in food security is an urgent need, rice, a non-traditional crop in most of countries in the region, has emerged as an important food crop that could extend the Green Revolution to the region following the introduction of New Rice for Africa (NERICA) in the early 2000s. Using data collected through a nationwide survey, this paper examines the possibility of rice green revolution by estimating the technical efficiency (TE) of rainfed rice farmers in Uganda and simulates how unfavorable climate changes affect it. The estimated stochastic frontier yield function showed that the mean TE was 65% for lowland and 60% for upland, and that the potential yield of rainfed rice cultivation was as high as 3 t·ha-1. However, the stochastic simulation of rainfall and rice yield revealed that unfavorable climate changes could erase the high potential in crop yield. Rainfed rice cultivation could be a leading sector for realizing Green Revolution in East Africa. It plays a critical role in this process to improve rice farmers’ TE, which is lower in the region than in Asia. Worsening climatic conditions, if occur, make this need even more imperative.

Evaluation of the Estrogenic Activity of Pueraria (Kudzu) Flower Extract and Its Major Isoflavones Using ER-Binding and Uterotrophic Bioassays  [PDF]
Tomoyasu Kamiya, Akira Takano, Yayoi Kido, Yuki Matsuzuka, Mayu Sameshima-Kamiya, Masahito Tsubata, Motoya Ikeguchi, Kinya Takagaki, Junei Kinjo
Pharmacology & Pharmacy (PP) , 2013, DOI: 10.4236/pp.2013.42036
Abstract: Pueraria flower extract (PFE) is a hot water extract of the Kudzu flower (Pueraria thomsonii). Tea made from dried Kudzu flower is widely used inChina, and PFE is utilized as a nutritional supplement inJapan. PFE contains unique isoflavones such as 6-hydroxygenistein 6,7-di-O-glucoside (6HGDG), tectorigenin 7-O-xylosylglucoside (TGXG), and tectoridin. 6HGDG is known to be metabolized into 6-hydroxygenistein, and TGXG and tectoridin are known to be metabolized into tectorigenin in the digestive tract. Isoflavones typically mimic the effects β-estradiol has on estrogen receptors (ERs) and may influence the female genital system in the case of excessive intake. As a result, the upper limit of safe daily consumption of soy isoflavones has been enforced inJapan. In the present study, ER-binding assays were performed using the EnBio estrogen receptor/cofactor assay system to compare the estrogenic activity of 6-hydroxygenistein and tectorigenin to that of the soy isoflavone genistein. In addition, uterotrophic bioassays were performed to investigate the estrogenic effects of PFE in vivo. The ER-binding assays revealed that the ER-binding affinities of 6-hydroxygenistein and tectorigenin were approximately 0.01 - 0.04 that of genistein. Soy isoflavone products also induced an increase
Hipercoagulabilidade e cancer de pulm?o
Marinho, Felipe Costa de Andrade;Takagaki, Teresa Yae;
Jornal Brasileiro de Pneumologia , 2008, DOI: 10.1590/S1806-37132008000500011
Abstract: the relationship between cancer and coagulopathy was suggested by trousseau nearly 150 years ago. later, it became more evident that oncologic patients are at a higher risk of experiencing thromboembolic events. this can be due to activation of the coagulation system either by neoplastic cells or by prescribed therapies (chemotherapy or surgical procedures). in fact, these events can constitute the first manifestation of cancer, and their recurrence, despite efficient anticoagulation, has been described. the coagulation system is normally activated in order to provide healing. in the presence of neoplasms, this complex system is activated as a response to multiple stimuli and seems to contribute to cancer progression. activation of the coagulation system has a greater effect on metastatic foci than on the primary tumor. however, most cancer victims die from complications caused by metastasis, which underscores the importance of this theme. in this area, various mechanisms have been described, creating promising perspectives for future treatments. the current success in using low-molecular-weight heparins against small cell lung cancer is encouraging. although the knowledge of those mechanisms is relatively incipient, many basic research and clinical studies are underway.
Nocardiose pulmonar e cutanea em paciente usuário de corticosteróide
Baldi, Bruno Guedes;Santana, Alfredo Nicodemos Cruz;Takagaki, Teresa Yae;
Jornal Brasileiro de Pneumologia , 2006, DOI: 10.1590/S1806-37132006000600019
Abstract: nocardiosis is a localized or disseminated infection caused by gram-positive bacteria of the genus nocardia. the infection most commonly affects the lungs, skin and central nervous system. nocardiosis principally occurs in individuals with cellular immunodeficiency and should be considered in the differential diagnosis when such individuals present respiratory, cutaneous or neurological alterations. herein, we report a case of pulmonary and cutaneous nocardiosis in a patient receiving oral corticosteroids to treat bronchiolitis obliterans accompanied by organizing pneumonia of unknown origin. after long-term treatment with sulfamethoxazole-trimethoprim, the clinical and radiological profile improved.
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