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Search Results: 1 - 10 of 15760 matches for " Yevgeniya Le "
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Immunocamouflage of latex surfaces by grafted methoxypoly(ethylene glycol) (mPEG): Proteomic analysis of plasma protein adsorption
Yevgeniya Le,Li Li,DunCheng Wang,Mark D. Scott
Science China Life Sciences , 2012, DOI: 10.1007/s11427-012-4290-2
Abstract: Grafting of methoxypoly(ethylene glycol) (mPEG) to cells and biomaterials is a promising non-pharmacological immunomodulation technology. However, due to the labile nature of cells, surface-plasma interactions are poorly understood; hence, a latex bead model was studied. PEGylation of beads resulted in a density and molecular weight dependent decrease in total adsorbed protein with a net reduction from (159.9±6.4) ng cm 2 on bare latex to (18.4±0.8) and (52.3±5.3) ng cm 2 on PEGylated beads (1 mmol L 1 of 2 or 20 kD SCmPEG, respectively). SDS-PAGE and iTRAQ-MS analysis revealed differential compositions of the adsorbed protein layer on the PEGylated latex with a significant reduction in the compositional abundance of proteins involved in immune system activation. Thus, the biological efficacy of immunocamouflaged cells and materials is mediated by both biophysical obfuscation of antigens and reduced surface-macromolecule interactions.
隐形微球表面吸附血浆蛋白的蛋白质组学分析
Yevgeniya Le, 李丽, 王敦成, Mark D. Scott
中国科学 生命科学 , 2012,
Abstract: 细胞和生物材料表面的甲氧基聚乙二醇(mPEG)修饰是一项具有广阔应用前景的非药理性免疫调节技术,但由于细胞的不稳定性,目前对其表面与血浆间相互作用的认识还不够深入.本实验采用相对稳定的乳胶微球作为模型,来研究其表面对血浆蛋白的吸附.结果显示,血浆总蛋白的吸附水平随着mPEG修饰浓度和分子量的增加而降低,未修饰的乳胶微球表面蛋白吸附量为(159.9±6.4)ng/cm2,mPEG修饰后,蛋白吸附量分别下降至(18.4±0.8)ng/cm2(1mmol/L2kDSCmPEG)和(52.3±5.3)ng/cm2(1mmol/L20kDSCmPEG).聚炳烯酰胺凝胶电泳(SDS-PAGE)和同位素标记相对和绝对定量的质谱技术(iTRAQ-MS)分析表明,在PEG修饰微球表面的蛋白质吸附层中,参与免疫系统活化的蛋白质丰度明显下降.因此,免疫修饰细胞和材料的生物学效应是通过降低抗原性及减弱表面与大分子间相互作用来实现的.
Representing the Translator: Making Sense of Translation in Cross-Language Qualitative Research. A Review of Bogusia Temple’s Recent Investigations in Research and Translation
Yevgeniya Traps
Graduate Journal of Social Science , 2009,
Abstract:
Development of a Methodology of Assessment in Accounting: Institutional Approach Развитие методологии оценки в бухгалтерском учете: институциональный подход
Popko Yevgeniya Yu.
Business Inform , 2013,
Abstract: The article thoroughly studies development of a methodology of assessment in accounting. The goal of the article is to justify priority nature of institutional interest in economic essence of specific assessment and reducing dependence of development of methodology of assessment from institutional competition of economic agents and institutional groups of the socio-economic environment. It shows that modern perception of assessment in accounting is narrow and requires re-consideration due to a set of basic provisions of institutional theory. Institutional approach allows making a conclusion that the problem of development of methodology of cost assessment goes beyond the limits of accounting system and lies not only in selection of a method of its carrying out, but also in understanding of what stimulates this selection and what are its consequences. It proves that any assessment contains interest of a certain economic agent (institutional group), which is the institutional motivation of development of methodology of assessment. В статье комплексно исследуется развитие методологии оценки в бухгалтерском учете. Целью статьи является обоснование приоритетности институционального интереса в экономической сущности учетной оценки и доведения зависимости развития методологии оценки от институциональной конкуренции экономических агентов и институциональных групп социально-экономической среды. Обосновано, что современное восприятие оценки в бухгалтерском учете является зауженным и требует переосмысления из-за призмы базовых положений институциональной теории. Институциональный подход позволяет осознать, что проблема развития методологии стоимостной оценки выходит за пределы учетной системы и заключается не столько в выборе способа ее осуществления, сколько в понимании, что стимулирует этот выбор и каковы его последствия. Доказано, что в любой оценке всегда заложен интерес определенного экономического агента (институциональной группы), который и является институциональной мотивацией развития методологии оценки.
Analysis of Specific Features of Development of Engineering Industry in Ukraine Анализ особенностей развития машиностроительной отрасли Украины
Nabok Yevgeniya V.
Business Inform , 2013,
Abstract: The article analyses the current state of engineering industry in Ukraine. It conducts analysis of the volume of production of engineering in Ukraine. It considers dynamics of the structure of industry and dynamics of investments into fixed assets of engineering industry in Ukraine. It analyses export and import of goods of the engineering industry. It considers reasons that influenced development of domestic engineering enterprises. It reveals problems of engineering enterprises. It offers ways of increase of competitiveness of products of engineering industry of Ukrainian enterprises. It proves that development of engineering enterprises is a necessary prerequisite of formation of potential of the country’s development in general. It marks main modern characteristics of engineering industry in Ukraine on the basis of the conducted analysis. В статье проанализировано текущее состояние машиностроительной отрасли Украины. Проведен анализ объема производства машиностроения Украины. Рассмотрена динамика структуры промышленности и динамика инвестиций в основной капитал машиностроительной области Украины. Проанализирован экспорт и импорт товаров машиностроительной промышленности. Рассмотрены причины, повлиявшие на развитие отечественных машиностроительных предприятий. Выявлены проблемы, с которыми сталкиваются машиностроительные предприятия. Предложены пути повышения конкурентоспособности продукции машиностроительной промышленности предприятий Украины. Доказано, что развитие машиностроительных предприятий является необходимой предпосылкой формирования потенциала развития страны в целом. На основе проведенного анализа указаны основные современные характеристики машиностроительной области Украины.
Remote Laboratory for Supporting e-Studies in Electronics
Vladimir M Cvjetkovic,Yevgeniya S Sulema
International Journal of Online Engineering (iJOE) , 2006,
Abstract: ¢ € ” E-learning becomes more and more popular today. It gives an opportunity for studying to persons who are not able to attend traditional courses and lectures for some reasons. In areas such as humanities or economical education, E-trainings provide a student with practically the same quality of learning materials. But in case of technical education a student must have traditional laboratory lessons to obtain practical knowledge on the subject. It concerns chemistry, physics, electronics and other technical disciplines. However, modern network technologies allow creating remote laboratories for supporting e-studies. The idea of using the remote lab for support of e-teaching electronics is discussed in the article. The structure and functionality of the remote laboratory are described. The software and hardware parts of the remote lab are considered and presented, as well.
Agarics and other macrobasidiomycetes from Ussuri River Valley
乌苏里江流域的伞菌及其它大型担子菌

BAU Tolgor,Yevgeniya M BULAKH,ZHUANG Jian-Yun,LI Yu,
图力古尔
,Yevgeniya M. BULAKH,庄剑云,李玉

菌物学报 , 2007,
Abstract: A checklist of 328 species in 132 genera of agarics and other macrobasidiomycetes from Ussuri River Valley in northeast of China and Far East of Russia is provided. Among them,Amanita baccata,Basidioradulum radula,Clitocybe fuscosquamula,Conocybe cyanopus,Crepidotus calolepis,Crepidotus subsphaerosporus,Crepidotus subverrucisporus,Datronia scutellata,Entoloma byssisedum,Entoloma pleopodium,Gymnopus hariolorum,Hygrocybe insipida,Hygrocybe virginea,Inocybe curvipes,Inocybe obscurobadia,Inocybe tenebrosa,Lepiota tomentella,Mycena acicula,Mycena adonis,Mycena amicta,Mycena meliigena,Mycena niveipes,Psathyrella spadicea,Resinomycena japonica,Rhodophyllus dysthales,Rhodophyllus strigosissimus,and Russula pelargonia are new to China.
Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and NOi Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway
Miensheng Chu,Yevgeniya Koshman,Rekha Iyengar,Taehoon Kim,Brenda Russell,Allen M. Samarel
Journal of Signal Transduction , 2012, DOI: 10.1155/2012/473410
Abstract: Intracellular nitric oxide (NOi) is a physiological regulator of excitation-contraction coupling, but is also involved in the development of cardiac dysfunction during hypertrophy and heart failure. To determine whether contractile activity regulates nitric oxide synthase (NOS) expression, spontaneously contracting, neonatal rat ventricular myocytes (NRVM) were treat with L-type calcium channel blockers (nifedipine and verapamil) or myosin II ATPase inhibitors (butanedione monoxime (BDM) and blebbistatin) to produce contractile arrest. Both types of inhibitors significantly reduced iNOS but not eNOS expression, and also reduced NOi production. Inhibiting contractile activity also reduced focal adhesion kinase (FAK) and AKT phosphorylation. Contraction-induced iNOS expression required FAK and phosphatidylinositol 3-kinase (PI(3)K), as both PF573228 and LY294002 (10 μM, 24 h) eliminated contraction-induced iNOS expression. Similarly, shRNAs specific for FAK (shFAK) caused FAK knockdown, reduced AKT phosphorylation at T308 and S473, and reduced iNOS expression. In contrast, shRNA-mediated knockdown of PYK2, the other member of the FAK-family of protein tyrosine kinases, had much less of an effect. Conversely, overexpression of a constitutively active form of FAK (CD2-FAK) or AKT (Myr-AKT) reversed the inhibitory effect of BDM on iNOS expression and NOi production. Thus, contraction-induced iNOS expression and NOi production in NRVM are mediated via a FAK-PI(3)K-AKT signaling pathway.
Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cells
Wu Ma, Tara Tavakoli, Eric Derby, Yevgeniya Serebryakova, Mahendra S Rao, Mark P Mattson
BMC Developmental Biology , 2008, DOI: 10.1186/1471-213x-8-90
Abstract: A reproducible protocol was used to generate highly homogenous neural progenitors or a mixed population of neural progenitors and neurons from hESCs. This defined adherent culture system allowed us to examine the effect of ECM molecules on neural differentiation of hESCs. hESC-derived differentiating embryoid bodies were plated on Poly-D-Lysine (PDL), PDL/fibronectin, PDL/laminin, type I collagen and Matrigel, and cultured in neural differentiation medium. We found that the five substrates instructed neural progenitors followed by neuronal differentiation to differing degrees. Glia did not appear until 4 weeks later. Neural progenitor and neuronal generation and neurite outgrowth were significantly greater on laminin and laminin-rich Matrigel substrates than on other 3 substrates. Laminin stimulated hESC-derived neural progenitor expansion and neurite outgrowth in a dose-dependent manner. The laminin-induced neural progenitor expansion was partially blocked by the antibody against integrin α6 or β1 subunit.We defined laminin as a key ECM molecule to enhance neural progenitor generation, expansion and differentiation into neurons from hESCs. The cell-laminin interactions involve α6β1 integrin receptors implicating a possible role of laminin/α6β1 integrin signaling in directed neural differentiation of hESCs. Since laminin acts in concert with other ECM molecules in vivo, evaluating cellular responses to the composition of the ECM is essential to clarify further the role of cell-matrix interactions in neural derivation of hESCs.Increasing evidence has shown that stem cell development requires a niche – a local microenvironment housing stem cells that regulates their self-renewal and fate in developing tissues or organs [1-5]. The regulatory signals from a niche are provided by niche cells, soluble factors and the extracellular matrix (ECM). Despite many studies showing that soluble factors such as FGFs, BMPs and Wnts can regulate stem cell behavior, the role of cell-m
Biological variability dominates and influences analytical variance in HPLC-ECD studies of the human plasma metabolome
Yevgeniya I Shurubor, Wayne R Matson, Walter C Willett, Susan E Hankinson, Bruce S Kristal
BMC Clinical Pathology , 2007, DOI: 10.1186/1472-6890-7-9
Abstract: Human plasma samples were biochemically analyzed using HPLC separations coupled with coulometric electrode array detection.We identified these markers/metabolites in human plasma, and then used them to determine which human samples represent blinded duplicates with 100% accuracy (N = 30 of 30). At least 47 of 61 metabolites tested were sufficiently stable for use even after 48 hours of exposure to shipping conditions. Stability of some metabolites differed between individuals (N = 10 at 0, 24, and 48 hours), suggesting the influence of some biological factors on parameters normally considered as analytical.Overall analytical precision (mean median CV, ~9%) and total between-person variation (median CV, ~50–70%) appear well suited to enable use of metabolomics markers in human clinical trials and epidemiological studies, including studies of the effect of caloric intake and balance on long-term cancer risk.After tobacco, over-nutrition is, arguably, the major cause of excess morbidity in developed countries, affecting a broad spectrum of diseases including cancer, cardio-/cerebrovascular disease, and type II diabetes. This association may be seen in both broad demographic groups, such as the American Cancer Society study group (900,000 U.S. adults)[1] and in more narrowly defined demographic groups, such as the Nurses' Health Study (NHS) group (122,000 U.S. female registered nurses) [2]. The difficulty of accurately assessing caloric intake and energy expenditure [3] has hampered studies relating to energy restriction, caloric balance, and caloric intake in both epidemiology and clinical nutrition. Several of the major hurdles in identifying biomarkers to address this and similar epidemiological problems are related to analytical (the lack of useful measurement standards) and methodological (the inability to distinguish individual physiology) issues [4-13]. Recent results have suggested the advantage of metabolomics approaches in clarifying these situations, at least
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