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Search Results: 1 - 10 of 20 matches for " Yarimar Carrasquillo "
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Hemispheric lateralization of a molecular signal for pain modulation in the amygdala
Yarimar Carrasquillo, Robert W Gereau IV
Molecular Pain , 2008, DOI: 10.1186/1744-8069-4-24
Abstract: The amygdala is a forebrain multinuclear structure with a well-established role in emotional processing [1,2]. Increasing evidence supports the role of the central nucleus of the amygdala (CeA) as a neural modulator of pain perception [3]. Previous work from our laboratory has identified the extracellular signal-regulated kinases (ERKs) as key molecules for the modulation of pain by the CeA in mice [4]. Biochemical experiments showed that ERK is activated in the CeA during persistent inflammation. ERK activation in the CeA was shown to be necessary for inflammation-induced peripheral hypersensitivity because acute pharmacological blockade of ERK activation in this amygdala nucleus reduced inflammation-induced peripheral tactile hypersensitivity. Furthermore, ERK activation in the amygdala was shown to be not only necessary for inflammation-induced peripheral hypersensitivity but also sufficient to induce peripheral tactile hypersensitivity in the absence of tissue injury.Interestingly, the biochemical data from our previous study showed that inflammation-induced ERK activation occurs in the right CeA independent of the side of peripheral inflammation (right or left hindpaw) [4]. These results suggest that modulation of pain by ERK activation in the CeA might be functionally lateralized to the right hemisphere. We tested this hypothesis in the present study by comparing the effects of acute blockade of ERK activation in the right versus the left amygdala when inflammation was induced in the right or the left hind-paw.To induce peripheral inflammation in mice, a 5% formalin solution was injected subcutaneously into the right or the left hind-paw as previously described [4]. Two hours after formalin injection into the hind-paw, the MEK inhibitor U0126 (1.5 nmoles), the inactive structural analog U0124 (1.5 nmoles) or vehicle (50% DMSO/Saline) were infused into the right or the left CeA. Formalin-induced mechanical sensitivity was measured 1 hr after intra-amygdala drug
De la decisión a la acción: estudio sobre el imperium en Tomás de Aquino
Francisco J. Romero Carrasquillo
Diánoia , 2012,
Rese a de "Women, Creole Identity, and Intellectual Life in Early Twentieth-Century Puerto Rico" de Magali Roy-Féquière
Rosa E. Carrasquillo
Centro Journal , 2006,
Resistencia al Sexo: Cultura y Personalidad
Yarimar Rosa Rodríguez,José Toro Alfonso
Revista Interamericana de Psicología , 2011,
Abstract: La manera en que una mujer responde a un acercamiento sexual, está regulada por normas sociales explícitas e implícitas. Estas normas están relacionadas a los patrones culturales en los que crecemos y nos desarrollamos, e impuestas por las instituciones sociales. Uno de los patrones de respuesta ante acercamiento sexuales que pueden observase en relaciones heterosexuales es el de prueba de resistencia al sexo (PRS). Este ha sido definido como el rechazo de un encuentro sexual, por parte de una mujer, aún cuando esta tiene el deseo de sostener el mismo (Muehlenhard & Hollabaugh, 1988). El presente trabajo de investigación tuvo como objetivo evaluar la ocurrencia de PRS en una muestra de estudiantes universitarias, evaluar las razones por las que ocurre PRS, y determinar si hay alguna relación entre las características de personalidad, factores culturales, y la creencia de que la PRS se da en el contexto de citas heterosexuales. Los resultados revelan que el 55.7% de la muestra total, se vio involucrada en una situación de PRS en el a o previo al estudio. También se identificaron factores que pueden contribuir al uso de esta herramienta como una alternativa para ponderar las decisiones en el contexto de citas entre hombres y mujeres.
SNF8, a member of the ESCRT-II complex, interacts with TRPC6 and enhances its channel activity
Carrasquillo Robert,Tian Dequan,Krishna Sneha,Pollak Martin R
BMC Cell Biology , 2012, DOI: 10.1186/1471-2121-13-33
Abstract: Background Transient receptor potential canonical (TRPC) channels are non-selective cation channels involved in receptor-mediated calcium signaling in diverse cells and tissues. The canonical transient receptor potential 6 (TRPC6) has been implicated in several pathological processes, including focal segmental glomerulosclerosis (FSGS), cardiac hypertrophy, and pulmonary hypertension. The two large cytoplasmic segments of the cation channel play a critical role in the proper regulation of channel activity, and are involved in several protein-protein interactions. Results Here we report that SNF8, a component of the endosomal sorting complex for transport-II (ESCRT-II) complex, interacts with TRPC6. The interaction was initially observed in a yeast two-hybrid screen using the amino-terminal cytoplasmic domain of TRPC6 as bait, and confirmed by co-immunoprecipitation from eukaryotic cell extracts. The amino-terminal 107 amino acids are necessary and sufficient for the interaction. Overexpression of SNF8 enhances both wild-type and gain-of-function mutant TRPC6-mediated whole-cell currents in HEK293T cells. Furthermore, activation of NFAT-mediated transcription by gain-of-function mutants is enhanced by overexpression of SNF8, and partially inhibited by RNAi mediated knockdown of SNF8. Although the ESCRT-II complex functions in the endocytosis and lysosomal degradation of transmembrane proteins, SNF8 overexpression does not alter the amount of TRPC6 present on the cell surface. Conclusion SNF8 is novel binding partner of TRPC6, binding to the amino-terminal cytoplasmic domain of the channel. Modulating SNF8 expression levels alters the TRPC6 channel current and can modulate activation of NFAT-mediated transcription downstream of gain-of-function mutant TRPC6. Taken together, these results identify SNF8 as a novel regulator of TRPC6.
Multiple Insulin Degrading Enzyme Variants Alter In Vitro Reporter Gene Expression
Olivia Belbin, Michael Crump, Gina D. Bisceglio, Minerva M. Carrasquillo, Kevin Morgan, Steven G. Younkin
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0021429
Abstract: The insulin degrading enzyme (IDE) variant, v311 (rs6583817), is associated with increased post-mortem cerebellar IDE mRNA, decreased plasma β-amyloid (Aβ), decreased risk for Alzheimer's disease (AD) and increased reporter gene expression, suggesting that it is a functional variant driving increased IDE expression. To identify other functional IDE variants, we have tested v685, rs11187061 (associated with decreased cerebellar IDE mRNA) and variants on H6, the haplotype tagged by v311 (v10; rs4646958, v315; rs7895832, v687; rs17107734 and v154; rs4646957), for altered in vitro reporter gene expression. The reporter gene expression levels associated with the second most common haplotype (H2) successfully replicated the post-mortem findings in hepatocytoma (0.89 fold-change, p = 0.04) but not neuroblastoma cells. Successful in vitro replication was achieved for H6 in neuroblastoma cells when the sequence was cloned 5′ to the promoter (1.18 fold-change, p = 0.006) and 3′ to the reporter gene (1.29 fold change, p = 0.003), an effect contributed to by four variants (v10, v315, v154 and v311). Since IDE mediates Aβ degradation, variants that regulate IDE expression could represent good therapeutic targets for AD.
Online measurements of the emissions of intermediate-volatility and semi-volatile organic compounds from aircraft
E. S. Cross,J. F. Hunter,A. J. Carrasquillo,J. P. Franklin
Atmospheric Chemistry and Physics Discussions , 2013, DOI: 10.5194/acpd-13-8065-2013
Abstract: A detailed understanding of the climate and air quality impacts of aviation requires detailed measurements of the emissions of intermediate-volatility and semi-volatile organic compounds (I/SVOCs) from aircraft. Currently both the amount and chemical composition of aircraft I/SVOC emissions remain poorly characterized. Here we characterize I/SVOC emissions from aircraft, using a novel instrument for the online, quantitative measurement of the mass loading and composition of low-volatility organic vapors. Emissions from the NASA DC8 aircraft were sampled on the ground, 143 m downwind of the engines and characterized as a function of engine power from ground idle (~4% maximum rated thrust) through 85% power. Results show that I/SVOC emissions are highest during engine-idle operating conditions, with decreasing but non-zero I/SVOC emissions at higher engine powers. Comparison of I/SVOC emissions with total hydrocarbon (THC) measurements, VOC measurements, and an established emissions profile indicates that I/SVOCs comprise 10–20% of the total organic gas phase emissions at idle, and an increasing fraction of the total gas phase organic emissions at higher powers. Positive matrix factorization of online mass spectra is used to identify three distinct types of I/SVOC emissions: aliphatic, aromatic and oxygenated. The volatility and chemical composition of the emissions suggest that unburned fuel is the dominant source of I/SVOCs at idle, while pyrolysis products make up an increasing fraction of the I/SVOCs at higher powers. Oxygenated I/SVOC emissions were detected at lower engine powers (≤30%) and may be linked to cracked, partially oxidized or unburned fuel components.
Construction and validation of Experiences Questionnaire on Violence in Couple and Family Relations in University Students [Desarrollo del Cuestionario de Experiencias de Violencia en las Relaciones de Pareja y Familia en Estudiantes Universitarios]
Angel A. Villafa?e Santiago,Maria Isabel Jimenez Chafey,Damaris De Jesus Carrasquillo,Robinson A. Vázquez Ramos
Universitas Psychologica , 2012,
Abstract: This study describes the process of developing the Experiences of Violence in Couple and Family Relationships in University Students Questionnaire, its psychometric properties and the results of the pilot study. The research design used for this study was a nonexperimental, transversal co relational design. The nonrandomized sample consisted of 267 students. The final version of the questionnaire consisted of 41 items and four sub-scales which measured experiences with violence in a relationship as an Aggressor and as a Victim, Observed between the Parents and in the Parent-child relationship as a victim. The total scale and the subscales obtained adequate reliability indexes. On average, the sample reported ten experiences with violence in different contexts. The results of this study contribute data on the prevalence of violence in college students’ romantic and family relationships which in turn, provide valuable information for planning prevention and early intervention efforts with this population.
Electrochemical Capacitance DNA Sensing at Hairpin-Modified Au Electrodes
Joel Rivera-Gandía,Maria del Mar Maldonado,Yarimar De La Torre-Meléndez,Edwin O. Ortiz-Quiles,Nella M. Vargas-Barbosa,Carlos R. Cabrera
Journal of Sensors , 2011, DOI: 10.1155/2011/735279
Abstract: An interfacial capacitance measurement electrochemical technique has been used for the sensing of self-assembled DNA hairpin probes (M. tuberculosis and B. anthracis) attached to Au electrodes. The double-layer capacitance ( ) was determined with electrochemical perturbations from 0.2?V to 0.5?V versus Ag/AgCl at a Au/M. tuberculosis DNA hairpin probe at surface coverage Au electrodes. The capacitance study was done at pH 7, which was necessary to maintain the M. tuberculosis and B. anthracis DNA probes closed during the electrochemical perturbation. Detailed experimental analysis carried out by repetitively switching the electrochemical potential between 0.2 and 0.5?V (versus Ag/AgCl) strongly supports the use of capacitance measurements as a tool to detect the hybridization of DNA targets. A large change in the capacitance deference between 0.2 and 0.5?V was observed in the DNA hybridization process. Therefore, no fluorophores or secondary transducers were necessary to sense a DNA target for both DNA hairpins. 1. Introduction The behavior of DNA attached onto metallic and nonmetallic surfaces via self-assembly with various chemistries (e.g., Au-S) may have applications in biomedical devices. For example, single-stranded DNA (ssDNA) self-assembled on a metallic interface such as gold [1, 2] or on nonmetals such as carbon nanotubes [3] and diamond [4–6] has potential use in DNA microarrays [7]. In addition, detection of DNA hybridization has been possible with techniques using different types of reporting, including fluorescence [8–11], chronocoulometry [12–14], surface plasmon resonance (SPR) [15, 16], colloidal labeling [17–19], and polarization modulation infrared reflection absorption spectroscopy [20]. Electroactive molecules can also be used to monitor the electron transfer mechanism during the hybridization process [21]. Here, we present a nonfaradaic electrochemical method based on capacitive measurement to sense DNA hairpin modification and hybridization. A nucleic acid probe has been developed to recognize specific DNA targets in solution [22]. These probes, called molecular beacons, are DNA hairpins with a fluorophore-quencher pair, which is completely unable to fluoresce when the two components are in close proximity (i.e., closed molecular beacon). When the molecular beacon spontaneously changes its conformation (like during hybridization), the fluorophore attached to one end of the molecule is no longer quenched as the quencher moves away. The capacity of this DNA hairpin has shown to discriminate between alleles with high specificity when
Evaluation of the Role of SNCA Variants in Survival without Neurological Disease
Michael G. Heckman, Alexandra I. Soto-Ortolaza, Nancy N. Diehl, Minerva M. Carrasquillo, Ryan J. Uitti, Zbigniew K. Wszolek, Neill R. Graff-Radford, Owen A. Ross
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0042877
Abstract: Background A variety of definitions of successful aging have been proposed, many of which relate to longevity, freedom from disease and disability, or preservation of high physical and cognitive function. Many behavioral, biomedical, and psychological factors have been linked with these various measures of successful aging, however genetic predictors are less understood. Parkinson's disease (PD) is an age-related neurodegenerative disorder, and variants in the α-synuclein gene (SNCA) affect susceptibility to PD. This exploratory study examined whether SNCA variants may also promote successful aging as defined by survival without neurological disease. Methods We utilized 769 controls without neurological disease (Mean age: 79 years, Range: 33–99 years) and examined the frequency of 20 different SNCA variants across age groups using logistic regression models. We also included 426 PD cases to assess the effect of these variants on PD risk. Results There was a significant decline in the proportion of carriers of the minor allele of rs10014396 as age increased (P = 0.021), from 30% in controls younger than 60 to 14% in controls 90 years of age or older. Findings were similar for rs3775439, where the proportion of carriers of the minor allele declined from 32% in controls less than 60 years old to 19% in those 90 or older (P = 0.025). A number of SNCA variants, not including rs10014396 or rs3775439, were significantly associated with susceptibility to PD. Conclusions In addition to its documented roles in PD and α-synucleinopathies, our results suggest that SNCA has a role in survival free of neurological disease. Acknowledging that our findings would not have withstood correction for multiple testing, validation in an independent series of aged neurologically normal controls is needed.
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