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Search Results: 1 - 10 of 120097 matches for " Yangjunqi Wang "
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In Situ Detection of the p53-E6 and pRb-E7 Complex Formation in EC109 Cell by Proximity Ligation Assay  [PDF]
Yangjunqi Wang, Jintao Li, Fan Li, Shuying Li, Rugang Zhong
Journal of Cancer Therapy (JCT) , 2019, DOI: 10.4236/jct.2019.104027
Abstract: Human papillomavirus is an important cancer factor in many cancers. E6E7 is the important viral oncogene and plays an important role. It is known that its function is to regulate p53 and pRb. In this paper, in situdetection was performed to determine the interaction between them; the research provides visual evidence of their interaction. Research methods: In situ PLA reaction was used to detect the relationship between two different protein. Results: The quantity of the HPV18E6 expression is much higher than the expression of HPV18E7 protein in HeLa and EC109 cells; the interaction between p53-E6 and pRb-E7 was clearly observed and this effect could be visualized by this method. Conclusion: Interaction of the HPV18E6 protein combined with p53 protein and HPV18E7 protein combined with pRb protein could be visualized in cell.
HPV18 E6 and E7 Intratumour Heterogeneity in Esophageal Cancer  [PDF]
Sara Khodahemmati, Maliha Gaffar, Jintao Li, Yangjunqi Wang, Xiaoli Wang, Zhixiang Zhou, Yi Zeng
Journal of Cancer Therapy (JCT) , 2019, DOI: 10.4236/jct.2019.105029
Abstract:

The development of esophageal cancer accompanied by the presence of human papillomavirus (HPV) DNA into the host genome. By evaluating the expression of this virus for tumor cell origin and also their cell grows and migrations, we examined esophageal cancer clonality in the context of intra-tumor heterogeneity. In this research, we have checked the expression of HPV18 E6 and E7 in different single cell clones by the manual cell picking method in the HPV positive esophageal cancer (EC109), EC109 cell line used as a negative control, and Hela cell line used as the positive control. Quantitative real-time PCR (QRT-PCR) was run to detect the expression levels of HPV E6 and E7, Cell Counting Kit-8 (CCK-8) assay was used to examine cell proliferation, invasion assays performed using Costar chambers and wounding assay to study cell migrations in vitro. We investigated the intra-tumor heterogeneity of HPV E6 and E7 in esophageal cancer and the evaluation of the growth and migrations at the clonal level, using 10 single cell clones. In particular clones, C7 & C10 displayed a highly variable expression in both HPV E6 and E7 and weak in four clones (C1, C3, C4, and C9) consequently, the cell invasion, proliferation, and migration increase with increasing the level of HPV expression and inverse. In conclusion, the resulting based on single cell cloning showed the relationship between HPV and cell growth and migration in esophageal cancer. Future study in HPV DNA integration needed to explore the mains specific integration site of HPV DNA in esophageal cancer and molecular monitoring of the HPV for future prevention researches and also effective therapeutic strategies.

Expressions of the Cell Cycle Relative Proteins in Esophageal Cancer Tissues  [PDF]
Sara Khodahemmati, Jintao Li, Hongwei Liu, Maliha Ghaffar, Yangjunqi Wang, Zhixiang Zhou, Yi Zeng
Journal of Cancer Therapy (JCT) , 2018, DOI: 10.4236/jct.2018.911071
Abstract: The aim of the research is to investigate the expression of the cell cycle relative proteins (P53, P16, Cyclin D1, and Ki67) in Esophageal Cancer (EC) patients of the Chaoshan area, China. In China, Chaoshan has the high incidence of EC. Different areas have shown different rate for expression of these proteins in EC. We investigated the expression of p53, p16, cyclinD1, and ki67 for the first time in Chaoshan. In this research, DNA was extracted from formalin fixed and paraffin embedded tissues of esophageal cancer (EC) patients. The expression level of proteins cycle was detected by using immunohistochemistry (IHC). And the data was checked by χ2 test or Fisher’s exact test of SPSS17.0. The positive immunohistochemical staining of p53, p16, cyclinD1, and ki67 were observed in 65.7% 39.2%, 69.1%, and 83.5% specimens respectively. There was a positive correlation between p53 positive staining and p16, cyclinD1, ki67 staining at p < 0.05. CyclinD1 has the high correlation with ki67 at p < 0.05. A significant inverse correlation was considered between the expression of p16 and cyclinD1 and there was no correlation observed between p16 and ki67. In Conclusion, this study demonstrated the high expression of p53, Cyclin D1 and Ki67 and low expression of P16 and the association of these cell cycle relative proteins in esophageal cancer are new data in Chaoshan area of China. Geographical distribution of EC on the molecular basis is revealed in this research.
Software Industry Cluster be Disagreement on Theory and Practice  [PDF]
Yang Wang, Luqian Wang
Intelligent Information Management (IIM) , 2010, DOI: 10.4236/iim.2010.210069
Abstract: In the view of traditional industry cluster theory, it is easy to copy the software industry cluster pattern, or it is easy to copy another Silicon Valley, due to low reliability of the resources and the guidance factors of locations in software industry. But it is much more difficult to copy a Silicon Valley mode practically than imaginatively and the difficulties of bringing up and supporting high-tech initiatives is more than theoretic anticipation. In China, the software companies have just gathered together geographically and therefore no initiative center can be formed. All these above signify that software industry cluster is distinct from the traditional industry clusters, but the cognition of the reasons of software industry cluster is not clear yet. Furthermore, reasonable explanations of the bewilderment in the economical practice of software industry cluster are urgently needed.
The Economic Growth Effect of Logistics Industry FDI Analysis  [PDF]
Yang Wang, Luqian Wang
iBusiness (IB) , 2010, DOI: 10.4236/ib.2010.24049
Abstract: Based on the direction of the international FDI changed, from manufacturing to service industries, we should concern about the influence of the introduction of China’s logistics industry FDI to the national economy. The paper sets up regression model to examine logistics FDI and GDP in two aspects of time series and growth rate, we find that the logistics FDI reaches a high correlation with GDP, and logistics FDI is one of the major driving forces of economic development. Therefore, China should expand the introduction of logistics FDI, improve the quality of foreign investment, develop the positive economic growth effect of logistics FDI and promote the change of China’s economic growth pattern to ensure the development of China’s economy.
The Predictive Effects of Online Communication on Well-Being among Chinese Adolescents  [PDF]
Jinliang Wang, Haizhen Wang
Psychology (PSYCH) , 2011, DOI: 10.4236/psych.2011.24056
Abstract: Based on theories and previous studies on online communication, we proposed two hypotheses to better under- stand the effects of online communication on subjective well-being with a sample of Chinese adolescents (n = 190). The results of liner regression analysis found that online communication was positively related to adoles- cents’ subjective well-being. For boys, the coefficient of determination of online communication on subjective well-being was .143, and for girls the coefficient of determination was .040, indicating that boys benefit more from online communication than do girls.
The Impact of Radical Prostatectomy on the Survival of Patients with Carcinosarcoma of the Prostate  [PDF]
Jue Wang, Fenwei Wang
Journal of Cancer Therapy (JCT) , 2011, DOI: 10.4236/jct.2011.24064
Abstract: Background: The objective of this study was to evaluate the impact of radical prostatectomy on carcinosarcoma of the prostate. Methods: Patients diagnosed with carcinosarcoma of the prostate from 1977 through 2007 were identified in the Surveillance, Epidemiology, and End Results (SEER) database. Outcomes were examined with Kaplan-Meier survival analysis and Cox models. The association between clinical and demographic characteristics and survival of carcinosarcoma of the prostate was examined. Results: A total of fifty-four cases of histology confirmed carcinosarcoma of the prostate were identified. Median age of the patients was 74 years (range 28 - 94). Of the patients with a known tumor stage, all but one patient had a locally advanced or distant stage; all of the patients with known histology grade had poorly or undifferentiated histology. Twenty (37%) patients underwent transurethral resection only and Fourteen (25.9%) patients underwent radical prostatectomy. Eight (14%) patients received radiation therapy in combination with surgery. The median cancer specific survival was 16 months (95% CI 0 - 32 months). 1-, 3-, and 5- year cancer specific survival rate were 55.1%, 32.3% and 21.1%. In a multivariate analysis, radical prostatectomy was found to be a significant prognostic factor for cancer-specific survival. Conclusions: Carcinosarcoma of the prostate commonly occurs in older patients and associated with aggressive disease and poor prognosis. Radical prostatectomy is the only treatment modality that significantly improves survival. Emphasis on early diagnosis and detection and multimodality therapy of this disease is needed to improve the outcome for patients with this malignancy.
A Research on the Relationship between FDI and Jiangsu’s Regional Economy Disparity  [PDF]
Baoqian Wang, Cheng Wang
Technology and Investment (TI) , 2012, DOI: 10.4236/ti.2012.33018
Abstract: Through reading and summarizing relevant articles, the authors analyze two different kinds of effects that FDI influence the regional economy. Location and digesting ability reflects such non-equilibrium. By using regression analysis, the authors discover, in south Jiangsu, which the unit FDI increase contributes most to the GDP development, with middle and north Jiangsu lagging behind. The regression coefficient is 1.198, 0.429 and 0.572 respectively. Considering the index of digestion ability, unit FDI increase and unit GDP contribution rate still share strong relevant relationship. However, the coefficients change. The effects that FDI stimulate GDP growth are weakened, been made up by the endogenous ability. The granger test shows that in south Jiangsu, there exists a cycle of accumulative effect, namely both the FDI increase and the GDP growth do well with each other. However, in the rest of Jiangsu province, such effect is not obvious. Considering the elements of location and digesting ability, this paper offers tactics for Jiangsu for a better coordinate of future development.
Causes of and Remedies for Chinglish in Chinese College Students’ Writings  [PDF]
Ping Wang, Weiping Wang
Open Journal of Modern Linguistics (OJML) , 2012, DOI: 10.4236/ojml.2012.22009
Abstract: Based on Interlanguage Theory and other theories, this study examines data from samples of Chinese college students’ writings and interviews with the students to explore the causes of and remedies for Chinglish (a term used to refer English with Chinese characteristics). It claims that Chinglish is mainly caused by syntactic transfer from Chinese, the influence of Chinese thought patterns, inadequate exposure to authentic English and insufficient practice in English writing. The interviews with the students showed that the proportion of L1 thinking decreased with the writer’s L2 development. The recommended remedies for Chinglish include raising students’ awareness of distinctions between English and Chinese, increasing their exposure to authentic English and practice in English writing, and adjusting their thought patterns.
An Entanglement Criterion for States in Infinite Dimensional Bipartite Quantum Systems  [PDF]
Yinzhu Wang, Danxia Wang
Journal of Quantum Information Science (JQIS) , 2012, DOI: 10.4236/jqis.2012.23013
Abstract: In this paper, an entanglement criterion for states in infinite dimensional bipartite quantum systems is presented. We generalize some of separability criterion that was recently introduced by Wu and Anandan in (Phys. Lett. A, 2002, 297, 4-8) to infinite dimensional bipartite quantum systems. In addition, we give an example aimed to illustrate the application of the theorem.
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