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Search Results: 1 - 10 of 73702 matches for " Yan-Feng Meng "
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Fabrication and Characterization of Manganese Ferrite Nanospheres as a Magnetic Adsorbent of Chromium
Li-Xia Yang,Feng Wang,Yan-Feng Meng,Qing-Hua Tang
Journal of Nanomaterials , 2013, DOI: 10.1155/2013/293464
Construction,expression,purification and identification of prokaryotic expression vector of MART-1 fusion protein
Zhao-ting MENG,Kai LI,Yan-feng YAN,Shun-chang JIAO
Medical Journal of Chinese People's Liberation Army , 2011,
Abstract: Objective To construct a prokaryotic expression plasmid containing a fusion gene of MART-1 expressing the His-MART-1 fusion protein in E.coli,and to purify the protein and identify the immunogenicity of His-MART-1.Methods The MART-1 coding sequence was amplified by polymerase chain reaction(PCR),and then cloned into the prokaryotic expression vector(pET-28b) containing His tag.The constructed vector,verified by restriction endonuclease digestion,PCR and DNA sequencing,was then transformed into E.coli for expression.The expression of MART-1 recombinant protein was induced by IPTG in E.coli,purified with Ni2+-NTA affinity chromatography method,and identified by SDS-PAGE and Western blotting.ELISA was used to detect the IFN-γ expression secreted by the His-MART-1 specific CD4+ T cells which recognized the His-MART-1 fusion protein presented by dendritic cells(DCs).Results The successful construction of recombinant plasmid was confirmed by restriction digestion,PCR and sequencing.The molecular weight of the purified fusion protein was identified as 13kD by SDS-PAGE,which was identical to the expected value.It was confirmed by western blotting that His-MART-1 fusion protein could be recognized by His monoclonal antibody.ELISA analysis showed that His-MART-1 fusion protein presented by DCs could induce IFN-γ secretion of MART-1 specific CD4+ T cells.Conclusion The recombinant plasmid of pET-28b-MART-1 has been successfully constructed.The expressed His-MART-1 fusion protein has been purified and the immunogenicity of inducing responses between DCs and CD4+ T cells has been determined.
Mth10b, a Unique Member of the Sac10b Family, Does Not Bind Nucleic Acid
Yan-Feng Liu,Nan Zhang,Hong-Wei Yao,Xian-Ming Pan,Meng Ge
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019977
Abstract: The Sac10b protein family is regarded as a group of nucleic acid-binding proteins that are highly conserved and widely distributed within archaea. All reported members of this family are basic proteins that exist as homodimers in solution and bind to DNA and/or RNA without apparent sequence specificity in vitro. Here, we reported a unique member of the family, Mth10b from Methanobacterium thermoautotrophicum ΔH, whose amino acid sequence shares high homology with other Sac10b family proteins. However, unlike those proteins, Mth10b is an acidic protein; its potential isoelectric point is only 4.56, which is inconsistent with the characteristics of a nucleic acid-binding protein. In this study, Mth10b was expressed in Escherichia coli and purified using a three-column chromatography purification procedure. Biochemical characterization indicated that Mth10b should be similar to typical Sac10b family proteins with respect to its secondary and tertiary structure and in its preferred oligomeric forms. However, an electrophoretic mobility shift analysis (EMSA) showed that neither DNA nor RNA bound to Mth10b in vitro, indicating that either Mth10b likely has a physiological function that is distinct from those of other Sac10b family members or nucleic acid-binding ability may not be a fundamental factor to the actual function of the Sac10b family.
Effects of the Chinese patent medicine, Honghua Injection, on platelet glycoprotein Ⅱb/Ⅲa receptors in patients with acute coronary syndrome: a randomized controlled trial
Yan-feng Zhu
Zhong Xi Yi Jie He Xue Bao , 2012,
Molecular Mechanism Underlying the Interaction of Typical Sac10b Family Proteins with DNA
Yan-Feng Liu, Nan Zhang, Xi Liu, Xinquan Wang, Zhi-Xin Wang, Yuanyuan Chen, Hong-Wei Yao, Meng Ge, Xian-Ming Pan
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0034986
Abstract: The Sac10b protein family is regarded as a family of DNA-binding proteins that is highly conserved and widely distributed within the archaea. Sac10b family members are typically small basic dimeric proteins that bind to DNA with cooperativity and no sequence specificity and are capable of constraining DNA negative supercoils, protecting DNA from Dnase I digestion, and do not compact DNA obviously. However, a detailed understanding of the structural basis of the interaction of Sac10b family proteins with DNA is still lacking. Here, we determined the crystal structure of Mth10b, an atypical member of the Sac10b family from Methanobacterium thermoautotrophicum ΔH, at 2.2 ?. Unlike typical Sac10b family proteins, Mth10b is an acidic protein and binds to neither DNA nor RNA. The overall structure of Mth10b displays high similarity to its homologs, but three pairs of conserved positively charged residues located at the presumed DNA-binding surface are substituted by non-charged residues in Mth10b. Through amino acids interchanges, the DNA-binding ability of Mth10b was restored successfully, whereas the DNA-binding ability of Sso10b, a typical Sac10b family member, was weakened greatly. Based on these results, we propose a model describing the molecular mechanism underlying the interactions of typical Sac10b family proteins with DNA that explains all the characteristics of the interactions between typical Sac10b family members and DNA.
Biodegradable and bioactive porous polyurethanes scaffolds for bone tissue engineering  [PDF]
Mei-Na Huang, Yuan-Liang Wang, Yan-Feng Luo
Journal of Biomedical Science and Engineering (JBiSE) , 2009, DOI: 10.4236/jbise.2009.21007
Abstract: Biodegradable porous polyurethanes scaffold have themselves opportunities in service, in-cluding controlled degradation rate, no-toxic degradation products. However, polyurethanes are lack of bioactive groups, which limits their application. This review gives the common modification methods, surface functionalization and blending modification. In finally, the review puts forward to the bulk modification as a new method to enhance the bioactivity of polyure-thanes.
African Maternal Origin and Genetic Diversity of Five Chinese Domestic Donkeys Breeds
Qian-Fu Gan,Shi-Xin Lin,Xue-Wu Liang,Hong-Li Meng,Hui Qiao,Yan-Feng Du,Fu-Rong Ke,Shu-Kai Zheng
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2011.3090.3094
Abstract: To clarify the origin of Chinese domestic donkeys, the researchers investigated the partial mitochondrial D-loop sequences of 145 samples from 5 native breeds. The results revealed two mitochondrial origins. In the analysis of sequences of 5 native breeds between previously published sequences from other countries/regions and sequences of Chinese domestic donkeys, the results indicated that the two lineages of Chinese domestic donkeys were from Africa and supported the African maternal origins of Chinese domestic donkeys. The population showed abundant mtDNA diversity.
Submilliampare threshold 1.3 μm vertical-cavity surface-emitting lasers

Lao Yan-Feng,Cao Chun-Fang,Wu Hui-Zhen,Cao Meng,Gong Qian,

物理学报 , 2009,
Abstract: 设计并研制了室温连续工作的单模13 μm垂直腔面发射激光器(VCSEL),阈值电流为051 mA,最高连续工作温度达到82℃,斜率效率为029 W/A.采用InAsP/InGaAsP应变补偿多量子阱作为有源增益区,由晶片直接键合技术融合InP基谐振腔和GaAs基GaAs/Al(Ga)As分布布拉格下反射腔镜,并由电子束蒸发法沉积SiO2/TiO2介质薄膜上反射腔镜形成13 μm VCSEL结构.讨论并分析了谐振腔模式与量子阱增益峰相对位置对器件性能的影响.

CAO Meng,WU Hui-Zhen,LAO Yan-Feng,CAO Chun-Fang,LIU Cheng,

红外与毫米波学报 , 2008,
Abstract: 采用气态源分子束外延系统生长了InAsP/InP应变多量子阱,研究了H 注入对量子阱光致发光谱的影响以及高温快速退火对离子注入后的量子阱发光谱的影响.发现采用较低H 注入能量(剂量)时,量子阱发光强度得到增强;随着H 注入能量(剂量)的增大,量子阱发光强度随之减小.H 注入过程中,部分隧穿H 会湮灭掉量子阱结构界面缺陷,同时H 也会对量子阱结构带来损伤,两者的竞争影响量子阱发光强度的变化.高温快速退火处理后,离子注入后的量子阱样品发光峰位在低温10K相对于未注入样品发生蓝移,蓝移量随着H 注入能量或剂量的增大而增加.退火过程中缺陷扩散以及缺陷扩散导致的阱层和垒层之间不同元素互混是量子阱发光峰位蓝移的原因.

刘艳鹏, 马生明, 朱立新, 曹达旺, 乐成生
LIU Yan-Feng
, MA Sheng-Meng, SHU Li-Xin, CAO Da-Wang, LE Cheng-Sheng

- , 2015, DOI: 10.13745/j.esf.2015.04.016
Abstract: 安徽兆吉口铅锌矿床位于长江中下游成矿带安庆—贵池矿集区南侧的东至县杨老尖—龙门尖地区,这一矿床的发现使皖西南地区地质找矿实现重大突破。文中利用Grant的Isocon图解法,研究活动元素的分布特征和迁移规律,以奠定兆吉口地区的地球化学勘查指标及成矿环境指标的研制基础,发现成矿元素Pb、Zn、Cu、Au、Ag、Mo及其伴生元素As、Bi、Sb和矿化剂元素S等从围岩向矿体部位带入程度增加,常量元素SiO2、Fe2O3、MgO、CaO、Na2O、K2O等在青白口系带出,于矿体部位带入。矿床系统的质量是净带入的,平均质量变化为4%;青白口系质量是净带出的,质量变化为-18%。依据元素迁移规律,试制元素迁移量三维地球化学图,认为研究区进一步找矿工作应当沿断裂带向北东和南西方向拓展,结合已有地质资料,对元素迁移进行了解释,并对矿床成因进行了讨论,认为试验矿床是由区域变质构造运动热液作用形成的,试验矿床只是区域变质构造运动热液活动共同形成的矿床(系列)的一部分,研究区具有良好的找矿前景。
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