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Search Results: 1 - 10 of 84 matches for " Yaima; Lucangioli "
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Determinación de residuos de deoxicolato de sodio en formulaciones vacunales por cromatografía electrocinética micelar
Yaima Merchán,Silvia Lucangioli,Clyde Carducci,Esther María Fajardo
Vaccimonitor , 2011,
Abstract: El deoxicolato de sodio (DCNa) es el surfactante por excelencia empleado en la industria biofarmacéutica para la solubilización de vesículas de membrana externa. Es bien conocida la importancia que reviste el control de este metabolito en materiales biológicos, debido a su alta toxicidad para el organismo humano. Para demostrar la presencia de bajas concentraciones de este metabolito en formulaciones vacunales es necesario el empleo de una metodología altamente selectiva, sensible, específica y reproducible. En el presente reporte se utilizó la cromatografía electrocinética micelar (MEKC) en un analizador capilar de iones (Water corp. Milford MA), con una detección a 185 nm, con lámpara de mercurio. Se empleó un capilar de sílica fundida (Waters Corp. Milford MA;se evaluó la pureza de dos lotes de deoxicolato de sodio y se analizaron 15 muestras de vesículas purificadas,ingrediente farmacéutico activo de formulaciones vacunales. Los datos fueron registrados y procesados con el software MillenniumTM (Waters Corp. Milford MA). Se determinó que los lotes de deoxicolato de sodio contenían 1,19% y 0,44% de ácido cólico contaminante y que el 93% de las muestras de vesículas purificadas tenían de 0 a 2,44 μg DCNa/100 μg de proteína. Los resultados obtenidos por MEKC fueron comparados con una modificaciónde una prueba cinética empleada para determinar ácidos biliares en sangre (Merckotest). El sistema MEKC mostró mejores resultados con respecto al Merkotest.
Determinación de residuos de deoxicolato de sodio en formulaciones vacunales por cromatografía electrocinética micelar
Merchán,Yaima; Lucangioli,Silvia; Carducci,Clyde; Fajardo,Esther María; Rico,Jeannete; Tamayo,Yolexis; Delgado,Ileana;
Vaccimonitor , 2011,
Abstract: the sodium deoxycholate (dcna) source is the surfactant used in the biopharmaceutical industry for the solubilization of outer membrane vesicles. it is well known the importance of control of this metabolite in biological materials due to its high toxicity for humans. to demonstrate significant small variations of this metabolite in vaccine formulations it is necessary to use a methodology highly selective, sensitive, specific and reproducible. in this report we used the micellar electrokinetic chromatography (mekc) in a capillary ion analyzer (water corp. milford ma) detection at 185 nm mercury lamp. it employed a fused silica capillary uncoated (waters corp. milford ma). we assessed the purity of 2 lots of sodium deoxycholate and analyzed 15 samples of purified vesicles active pharmaceutical ingredient vaccine formulations. data were recorded and processed with software millennium tm (waters corp. milford ma). it was found that lots of sodium deoxycholate containing 1.19 and 0.44% cholic acid and contaminate that 93% of the purified vesicles samples were from 0 to 2.44 mg protein dcna/100 μg. meck's results were compared with a kinetic test used to determine bile acids in blood (merckotest). meck system showed better results regarding the merkotest.
Development of Amine Capillary Column Applied to the Analysis of Basic Compounds by Electrochromatography  [PDF]
Sabrina Flor, Juan Martin Huala, Valeria Tripodi, Silvia Lucangioli
Journal of Analytical Sciences, Methods and Instrumentation (JASMI) , 2015, DOI: 10.4236/jasmi.2015.54008
Abstract: An amine capillary column was developed in a fused-silica capillary as stationary phase and applied to separation of basic compounds by capillary electrochromatography (CEC). The functionalized capillary was prepared by inmobilization of 3-Aminopropyl)triethoxysilane (APTES). The CEC conditions including APTES and buffer concentration, pH and applied voltage were investigated to obtain the optimal CEC system for the separation of three anesthetics as basic test molecules. The capillary column provided an efficiency of up to 20,000 plates/m. Lidocaine, ketamine and xilacine were baseline separated under the running conditions with 10 mM Na2HPO4 pH 9.0 as BGE. The applied voltage was 5 kV temperature was set at 25oC and UV detection was performed. The resolutions were 4.97 and 1.53 for ketamine, lidocaine and xilacine, respectively. The column used in CEC mode showed better separation of the anesthetics compared with those used in the capillary zone mode. The comparison with reversed stationary phases used in HPLC in terms of resolution (Rs), sensitivity (LOD), efficiency (N), precision (RSD), asymmetry (T) was also performed. This study provided an alternative way for the CEC separation of basic compounds and demonstrated the improvement in the chromatographic parameters.
Uso de la acupuntura en el ni?o tartamudo
Figueras,Yaima; Leiva,Damayantis;
Revista Cubana de Enfermer?-a , 2000,
Abstract: the incidence of stutterer patients and the importance of their incorporation to society, are observed during the logophoniatric practice in the different medical services, therefore the present investigative work is performed with the objective of assessing the importance of acupuncture in the rehabilitation of these patients. 33 patients with diagnosis of defined stuttering, as investigative group were chosen as well as 22 patients (control group) with similar diagnosis and general characteristics of the logopaedia and phoniatry service of the pediatric hospital of centro habana municipality. partial conclusions were reached since only the first phase of the research was analyzed. the favorable results that can be reached by applying in unison both treatments, logophoniatry and acupuncture are expressed.
Uso de la acupuntura en el ni o tartamudo
Yaima Figueras,Damayantis Leiva
Revista Cubana de Enfermer?-a , 2000,
Abstract: Se observa durante la práctica logofoniátrica en los diferentes servicios médicos, la incidencia de pacientes tartamudos y la importancia de su incorporación a la sociedad, por lo que se realiza este trabajo investigativo con el objetivo de valorar la importancia de la acupuntura en la rehabilitación de estos pacientes. Se escogieron 33 pacientes con diagnóstico de tartamudez definida, como grupo investigativo y como grupo control 22 pacientes con igual diagnóstico y características generales del servicio de logopedia y foniatría del Hospital Pediátrico de Centro Habana. Se llegó a conclusiones parciales ya que solamente se analiza la primera fase de la investigación. Se expresan los resultados favorables que pueden alcanzarse aplicando al unísono ambos tratamientos, logofoniátricos y acupuntural. The incidence of stutterer patients and the importance of their incorporation to society, are observed during the logophoniatric practice in the different medical services, therefore the present investigative work is performed with the objective of assessing the importance of acupuncture in the rehabilitation of these patients. 33 patients with diagnosis of defined stuttering, as investigative group were chosen as well as 22 patients (control group) with similar diagnosis and general characteristics of the logopaedia and phoniatry service of the Pediatric Hospital of Centro Habana municipality. Partial conclusions were reached since only the first phase of the research was analyzed. The favorable results that can be reached by applying in unison both treatments, logophoniatry and acupuncture are expressed.
Sistema cromatográfico miniaturizado para la determinación de coenzima Q10 en plasma, músculo y plaquetas
Contin,Mario; Martinefski,Manuela; Lucangioli,Silvia; Tripodi,Valeria;
Acta bioqu?-mica cl?-nica latinoamericana , 2011,
Abstract: coenzyme q10 (coq10) is an essential component of the respiratory chain involved in cellular energy production. moreover, it is considered a potent antioxidant that acts in the prevention of oxidative dna, biological membrane and lipoprotein damage. recently, it has been demonstrated that its deficiency is implicated in several diseases such as mitochondrial, heart and muscle diseases, cancer and during treatment with statins. since coq10 therapy is beneficial in these diseases, their levels should be monitored during treatment. the determination of coq10 levels in deficiency diagnosis and post-treatment control is a major analytical challenge since it is a highly hydrophobic molecule that iseasily oxidized, and its endogenous concentration is extremely low even in control subjects. as a result, an analytical method with high sensitivity and an adequate sample preparation to avoid losses during processing must be used. although coq10 is often analyzed in plasma, it has recently been shown that in some cases, plasma coq10 does not accurately reflect the intracellular concentration; and it would be necessary to determine it in other matrices such as platelets and muscle. in this paper, adequate sample preparations were determined for the determination of coq10 in plasma, platelets and muscle that require small quantities of sample using a miniaturized chromatographic analytical system, that is simple and has high sensitivity, precision and accuracy that will enable the diagnosis and monitoring of the diseases involving coq10 deficiency.
Sistema cromatográfico miniaturizado para la determinación de coenzima Q10 en plasma, músculo y plaquetas Sistema cromatográfico miniaturizado para a determina o de coenzima Q10 em plasma, músculo e plaquetas Miniaturized chromatographic system for determination of coenzyme Q10 in plasma, muscle and platelets
Mario Contin,Manuela Martinefski,Silvia Lucangioli,Valeria Tripodi
Acta bioqu?-mica cl?-nica latinoamericana , 2011,
Abstract: La coenzima Q10 (CoQ10) es un componente esencial de la cadena respiratoria que interviene en la producción celular de energía. A su vez, es considerada un potente antioxidante que actúa en la prevención del da o oxidativo del ADN, membranas biológicas y lipoproteínas. En los últimos tiempos se ha demostrado que su deficiencia está implicada en varias patologías como enfermedades mitocondriales, cardíacas y musculares, cáncer y durante el tratamiento con estatinas. Como la terapia con CoQ10 resulta beneficiosa en dichas patologías, sus niveles deben ser monitoreados durante el tratamiento. La determinación de los niveles de CoQ10 para el diagnóstico de su deficiencia y control post tratamiento representa un gran desafío analítico puesto que es una molécula de elevada hidrofobicidad, fácil oxidación y su concentración endógena es extremadamente peque a aun en sujetos controles. Debido a ello, es necesario utilizar un método analítico de elevada sensibilidad y una preparación de muestra adecuada para evitar pérdidas durante su procesamiento. Si bien CoQ10 es frecuentemente analizada en plasma, recientemente se ha demostrado que, en algunos casos, la CoQ10 plasmática no refleja exactamente la concentración intracelular, por lo que sería necesario realizar su determinación en otras matrices como plaquetas y músculo. En el presente trabajo se han desarrollado preparaciones de muestras adecuadas para la determinación de CoQ10 en plasma, plaquetas y músculo que requieren peque as cantidades de muestra utilizando un sistema analítico cromatográfico miniaturizado, sencillo, de elevada sensibilidad, precisión y exactitud que permitirá un diagnóstico y seguimiento apropiado de las patologías que involucran la deficiencia de CoQ10. A coenzima Q10 (CoQ10) é um componente essencial da cadeia respiratória que intervém na produ o celular da energia. Por sua vez, é considerada um potente antioxidante que age na preven o do dano oxidativo ao DNA, membranas biológicas e lipoproteínas. Nos últimos tempos se tem demonstrado que sua deficiência está envolvida em várias patologias como doen as mitocondriais, cardíacas e musculares, cancer e durante o tratamento com estatinas. Como a terapia com CoQ10 resulta benéfica em tais patologias, seus níveis devem ser monitorados durante o tratamento. A determina o dos níveis de CoQ10 para o diagnóstico de sua deficiência e controle pós-tratamento representa um grande desafio analítico visto que é uma molécula de elevada hidrofobicidade, fácil oxida o e sua concentra o endógena é extremamente pequena mesmo em sujeitos controle. Por
In Search of an Accurate Evaluation of Intrahepatic Cholestasis of Pregnancy
Manuela Martinefski,Mario Contin,Silvia Lucangioli,Maria Beatriz Di Carlo
Scientifica , 2012, DOI: 10.6064/2012/496489
Abstract:
Bioremediation: a tool for the management of oil pollution in marine ecosystems
Barrios San Martín,Yaima;
Biotecnolog?-a Aplicada , 2011,
Abstract: during the last decades the course of the anthropogenic contamination of marine ecosystems has paralleled that of the oil industry. spills of crude oil and its derivatives can have short, medium and long-term negative consequences, and the elimination of pollutants by natural means may take years or even longer. bioremediation is an emergent branch of environmental biotechnology that is often used to accelerate this process and guarantees the reparation of damaged ecosystems, based on harnessing the metabolic capabilities of bacteria, fungi, yeast, algae and microbial mats to degrade oil hydrocarbons. bioremediation follows two main strategies: the stimulation of indigenous microbial populations, known as biostimulation, and bioaugmentation, the introduction of viable microbial populations. choosing one or another depends on the analysis of abiotic and biotic factors influencing the biodegradation process; the former refers to factors related with the pollutant and environmental conditions, while the latter encompasses all factors that have to do with microbial populations. the development of bioremediation has led to the appearance of commercially available products for spill cleanup: fertilizers containing biostimulating nutrients, bioproducts based on microorganisms, and chemical products to stimulate the growth of the microbial populations involved in the process of biodegradation.
In Search of an Accurate Evaluation of Intrahepatic Cholestasis of Pregnancy
Manuela Martinefski,Mario Contin,Silvia Lucangioli,Maria Beatriz Di Carlo,Valeria Tripodi
Scientifica , 2012, DOI: 10.6064/2012/496489
Abstract: Until now, biochemical parameter for diagnosis of intrahepatic cholestasis of pregnancy (ICP) mostly used is the rise of total serum bile acids (TSBA) above the upper normal limit of 11?μM. However, differential diagnosis is very difficult since overlapped values calculated on bile acids determinations, are observed in different conditions of pregnancy including the benign condition of pruritus gravidarum. The aim of this work was to determine the better markers in ICP for a precise diagnosis together with parameters associated with severity of symptoms and treatment evaluation. Serum bile acid profiles were evaluated using capillary electrophoresis in 38 healthy pregnant women and 32 ICP patients and it was calculated the sensitivity, specificity, accuracy, predictive values and the relationships of certain individual bile acids in pregnant women in order to replace TSBA determinations. The evaluation of the results shows that LCA and UDCA/LCA ratio provided information for a more complete and accurate diagnosis and evaluation of ICP than calculation of solely TSBA levels in pregnant women. 1. Introduction Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific liver disease and it takes place in the second or third trimester of gestation and it spontaneously disappears after delivery [1, 2]. ICP is characterized by generalized skin pruritus and abnormal liver function, and it is associated with increased fetal distress, premature deliveries, and perinatal mortality and morbidity. Therefore, an early and accurate diagnosis of a risky pregnancy produced by ICP is essential [3–5]. Usually, diagnosis of ICP is based on pruritus with mild or moderate elevated levels of amino transferases and/or raised total serum bile acids (TSBAs) [6]. However, it is often difficult to accomplish an accurate diagnosis by performing solely routine laboratory tests because they are also altered in some other conditions of pregnant women. In fact, the existence of subclinical cholestasis during pregnancy may also impair the identification of the disease. Moreover, pruritus in pregnancy is a common symptom but it could be the only evidence in ICP, so it is necessary to discriminate women with ICP from those with benign condition of pruritus gravidarum [5, 7]. Furthermore, a subgroup of asymptomatic pregnant women with high levels of TSBA, and normal liver function tests but not showing pruritus, has recently been classified as asymptomatic hypercholanemia of pregnancy (AHP) [7–10]. In agreement with some authors [4, 5, 7, 11], we have reported that the individual
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