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Maturation Rate of Ovine Oocytes from Different Reproductive Status and Maturation Medium
ARIEF BOEDIONO,YULNAWATI,MOHAMAD AGUS SETIADI
HAYATI Journal of Biosciences , 2006,
Abstract: The aim of the present study was to determine the number of follicles, oocyte quality and maturation rate of oocytes from pairs of ovary with different reproductive status in two maturation medium, TCM-199 as control and CR1aa as treatment. The pairs of ovary were classified into four groups: (i) ovaries with corpus luteum (CL) and dominant follicle (DF), (ii) ovaries with CL, without DF, (iii) ovaries with DF, without CL, (iv) ovaries without both CL and DF. Results of the experiment revealed that the greatest number of follicles was observed from ovary with CL without DF (15.88 + 10.68), although not significantly different (P > 0.05) with other status of ovaries. The lowest number (P < 0.05) of A grade oocytes was found from ovary with DF without CL (1.20 + 1.10). The percentage of Metaphase II was highest in TCM-199 (75.51%) with oocytes from ovaries with CL and DF, and the lowest with oocytes from ovaries with DF without CL in TCM-199 and CR1aa (42.86 and 30.95%). The study suggested that the number of oocytes with A grade were influenced by the reproductive status of ovaries. The maturation rate of A grade oocytes was influenced by the quality of oocytes and the composition of maturation medium.
Quality of Epididymal and Ejaculated Sperms of Spotted Buffalo in Dextrose Supplemented Extender
YULNAWATI,MUHAMMAD GUNAWAN,HERA MAHESHWARI,MUHAMMAD RIZAL
HAYATI Journal of Biosciences , 2010,
Abstract: Quality of epididymal and ejaculated sperms of spotted buffalo in three different extenders (i.e. Andromed, Andromed supplemented with 0.2% dextrose, and Andromed supplemented with 0.4% dextrose) was studied. The results showed that there was no significantly different (P > 0.05) quality of ejaculated and epididymal sperms. The motility percentage of post thawing epididymal and ejaculated sperms was 41-46% and 41-45%, respectively. And percentage of cytoplasmic membrane integrity of the post thawing epididymal and ejaculated sperms was 66-67% and 47-54% respectively. There was also no significant different (P > 0.05) in the percentage of cytoplasmic membrane integrity between the extenders of both ejaculated and epididymal sperms. This result suggested that epididymal sperms were reliable for artificial insemination as good as ejaculated sperms.
VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER
YULNAWATI,H. MAHESHWARI,HERDIS,M. RIZAL
BIOTROPIA : the Southeast Asian Journal of Tropical Biology , 2009,
Abstract: h e objective of this study was to obtain the viability and plasma membrane integrity of the spotted buff alo epididymal sperm after addition of dextrose into Andromed extender. Spermatozoa that have been collected from cauda epididymis were diluted with Andromed extender as control (K) and Andromed + 0.2% dextrose (P1) and Andromed + 0.4% dextrose (P2) as treatments. h e results showed that the quality of epididymal spermatozoa decreased during cryopreservation process. h e percentage of motility after thawing in P1 (46%) and P2 (46.67%) were signifi cantly higher (P<0.05) compared to K (41%) as well as the percentage of live sperm in P1 (58.8%) and P2 (60%) compared to K (52.2%). h e percentage of membrane integrity in P1, P2 and K were 67.4; 66.8 and 68 %, respectively. In conclusion, the addition of 0.2 and 0.4% of dextrose into Andromed acted as an extra cellular cryoprotectant and could maintain the viability and membrane integrity of the spotted buff alo epididymal spermatozoa after thawing.
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