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Search Results: 1 - 10 of 240787 matches for " YUAN Xiao-Fan ZHAO Bing WANG Yu-Chun "
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Application of Rare Earth Elements in Medicinal Plant Cell and Tissue Culture
稀土元素在药用植物细胞和组织培养中的应用

YUAN Xiao-Fan ZHAO Bing WANG Yu-Chun,
袁晓凡
,赵兵,王玉春

植物学报 , 2005,
Abstract: Here we discussed the effects of rare earth elements on the growth of medicinal plant cell and tissue cultured in vitro and biosynthesis of secondary metabolites in the cultured materials.Increased data has shown that the application of rare earth elements has splendid prospects in medicinal plant cell and tissue culture.
Promotion of Cell Growth and Flavonoids Production in Saussurea medusa Cell Suspension Cultures by Rare Earth Elements
稀土元素对水母雪莲细胞生长及黄酮类化合物合成的影响

YUAN Xiao-fan,WANG Qian,ZHAO Bing,WANG Yu-chun,
袁晓凡
,王谦,赵兵,王玉春

过程工程学报 , 2004,
Abstract: Cell suspension culture of Saussurea medusa was cultivated with either Ce3+, La3+, Nd3+ or a mixture of rare earth elements (MRE) supplemented. After 20 d culture, moderate concentrations of Ce3+, La3+ and MRE can promote cell growth and flavonoids biosynthesis, while Nd3+ presented an inhibitting effect. Ce3+ at 0.025 mmol/L, gave the highest cell dry weight (17.7 g/L) and flavonoids production (942 mg/L) with both NAA and 6-BA in the culture medium. They were 34% and 67% higher than those without Ce3+ addition, respectively. Ce3+ at 0.005~0.05 mmol/L or the mixture of rare earth elements at 0.01~0.025 mmol/L, can substitute for 6-BA in promoting cell growth and the biosynthesis of total flavonoids of S. medusa while without 6-BA in the culture medium. When NAA were removed from the culture medium, none of the four rare earth elements can substitute for it in promoting S. medusa cell growth and the flavonoids biosynthesis. The kinetic curves of cell biomass, flavonoids production, residual sugar, NH4+ and NO3- concentration with or without 0.025 mmol/L Ce3+ in the culture medium were also obtained. The mechanism of the promotion effect of Ce3+ on cell growth and the biosynthesis of total flavonoids of Saussurea medusa was explained in some degree.
Analysis of Land Utilization, Energy Consumption, Environmental Effect and Water Consumption in Production of Fuel Ethanol with Biomass
燃料乙醇生产用生物原料的土地使用、能耗、环境影响和水耗分析

KONG De-zhu,WANG Yu-chun,SUN Jian,YUAN Xiao-fan,WANG Xiao-dong,XIAO Jie,ZHAO Bing,
孔德柱
,王玉春,孙健,袁晓凡,王晓东,肖杰,赵兵

过程工程学报 , 2011,
Abstract: 在引入生物质分配比率的基础上,从土地使用率、能耗、环境和耗水量4个方面分析了玉米秸秆、木薯和甜高粱3种生物质原料生产燃料乙醇的过程,得到了3种生物质发展潜力数据. 结果表明,玉米秸秆作为原料,按产出乙醇能计,土地使用率最高可达563.40 GJ/ha,投入能量最低,仅为22.68 MJ/GJ,环境影响最小,耗水量最少,只有8 m3/GJ,是最有开发前景的原料. 甜高粱总体上优于木薯,但耗水量太大,不利于长远发展.
Research Progress in Culture Conditions and Fermentation Technology of Bacillus mucilaginosus
胶质芽孢杆菌培养条件及发酵工艺的研究进展

WANG Xue,YUAN Xiao-fan,ZHAO Bing,WANG Xiao-dong,WANG Yu-chun,
王雪
,袁晓凡,赵兵,王晓东,王玉春

过程工程学报 , 2010,
Abstract: 对胶质芽孢杆菌在微生物肥料、矿物分解和生物絮凝领域中培养条件及发酵工艺的研究进展进行了综述. 在微生物肥料方面综述了以高产量芽孢为目的的培养工艺及生物反应器发酵的研究;在矿物分解方面综述了以分解矿物和浸出矿物元素为目的的土壤矿物分解和矿石分解条件的研究;在生物絮凝方面综述了以提高絮凝率为目的的培养工艺和絮凝环境条件的研究. 在对现有研究总结的基础上,指出通过发酵模型的建立、流变学特性的研究指导发酵过程调控及将胶质芽孢杆菌应用于土壤改良是进一步研究和探索的方向.
Effect of Submerged/Unsubmerged Period on Cell Culture of Saussurea medusa in a Novel Air-lift Bioreactor
新型气升式反应器中浸没/非浸没周期对水母雪莲细胞培养的影响

YUAN Xiao-fan,CHEN Shu-an,ZHAO Bing,WANG Yu-chun,LIU De-ming,
袁晓凡,陈书安,赵兵,王玉春,刘德明

过程工程学报 , 2003,
Abstract: The alternant submerged/unsubmerged cell culture of Saussurea medusa in a novel 1.8 L periodically submerged air-lift bioreactor was investigated. The bioreactor contains two layers of meshes. They provide support for cells in unsubmerged culture, but when being pulled slant the culture can be released by circulating medium from the meshes for submerged culture. The effects of the submerged/unsubmerged period on cell growth, biosynthesis of flavonoids and cell aggregate were studied. It was found that 5 min/4 h was the optimum submerged/unsubmerged period when the culture was carried out at air flow rate of 40 L/h and an inoculum (DW) of 2-3 g/L. After 20 d culture, the maximum biomass and production of flavonoids reached 9.6 g/L and 338 mg/L respectively, and the distribution of cell aggregates was the optimal as well.
Rapid and High Frequent Induction of Saffron Corms
番红花球茎的快速高频诱导

CHEN Wen-hao,OU Yuan,ZHAO Bing,WANG Xiao-dong,WANG Yu-chun,
陈文浩
,欧元,赵兵,王晓东,王玉春

过程工程学报 , 2007,
Abstract: The effects of exogenous plant hormones and activated carbon (AC) on the development of saffron corms in vitro were studied. Both hormone concentration and AC level influenced corm induction. When 1/2 Murashige and Skoog medium was supplemented with low concentration of hormones, corms were induced quickly while big corms formed slowly at high concentration of hormones. The addition of AC decreased the concentration of free hormones and the time of corm development significantly, while the slow release of hormones raised corm size. In the medium with optimal 0.5 g/L AC, high induction rate (85.7%) and big corms (0.53 g average weight) were obtained in 5 weeks when 5 mg/L naphthalene acetic acid and 5 mg/L 6-benzylaminopuine were added to the medium.
Effects of Light Quality on Maca Callus Growth and Differentiation
光质对玛咖愈伤组织生长、分化的影响

WANG Ya-li,WANG Xiao-dong,ZHAO Bing,WANG Yu-chun,
王亚丽
,王晓东,赵兵,王玉春

过程工程学报 , 2007,
Abstract: 研究了在组织培养过程中光质对玛咖愈伤组织生长、分化及细胞内糖代谢关键酶的影响.在红光、黄光和白光下培养25 d, 玛咖愈伤组织的干重明显高于在绿光和蓝光下.在分化培养基上,红光、黄光和白光条件下玛咖愈伤组织的出芽率达78%~82%,而在绿光和蓝光下出芽率几乎为0.进一步研究表明,红光、黄光和白光条件下糖代谢中3种关键酶(葡萄糖-6-磷酸酶、己糖激酶、苹果酸脱氢酶)的活性明显高于绿光和蓝光条件下,与愈伤组织生长、分化情况相关联,说明细胞内的糖代谢受光质调节并参与了对愈伤组织生长和分化的调节.
Screening of Crocus sativus L. Callus Lines for Crocin Production
产藏红花素1(crocin)愈伤组织的诱导及其细胞系的筛选

CHEN Shu-An WANG Xiao-Dong ZHAO Bing WANG Yu-Chun,
陈书安
,王晓东,赵兵,王玉春

植物学报 , 2004,
Abstract: 对藏红花(Crocus sativus L.) 愈伤组织的诱导条件进行了优化。结果表明:MS 是藏红花芽愈伤组织的最佳诱导培养基,而B5是叶子和花愈伤组织的最佳培养基。藏红花芽、叶和花愈伤组织的最佳诱导温度分别是18℃、25℃和21℃。光照是叶子愈伤组织诱导的有利因素,但不利于芽和花愈伤组织的诱导。1.5~2.0 mg.L-1 NAA和0.25 mg.L-1 6-BA是愈伤组织诱导的最佳激素组合。通过目视法和HPLC方法, 从229株细胞系中筛选出细胞系Corm1,其藏红花素1 的含量是1 677 mg.g-1,生长较快,且不易褐化。为采用植物细胞工程法解决藏红花素1资源短缺问题打下了基础。
Cryopreservation of Cistanche deserticola Callus
肉苁蓉愈伤组织的超低温保藏方法

GE Feng,WANG Xiao-dong,ZHAO Bing,WANG Yu-chun,
葛锋
,王晓东,赵兵,王玉春

过程工程学报 , 2006,
Abstract: 提出了一个优化的简单的超低温保藏方法,成功地运用于肉苁蓉愈伤组织的低温保藏. 为了获得最佳的实验结果,肉苁蓉愈伤组织首先在添加了6%二甲亚砜的B5培养基中进行预培养,然后用玻璃化保护剂在25℃处理20 min,最后投入液氮中进行冷冻. 玻璃化保护剂的成分为30%(ψ)甘油 15%(ψ)乙二醇 10%(ψ)二甲亚砜 0.5 mol/L蔗糖. 冷冻后的愈伤组织在30℃的水浴中迅速解冻,接着用25℃的1.0 mol/L蔗糖溶液洗净愈伤组织上附着的玻璃化保护剂,最后在B5培养基上对愈伤组织进行恢复性培养. 经过上述冻存处理的肉苁蓉愈伤组织存活率可达86%. 恢复培养5个月后,愈伤组织中苯乙醇糖甙类化合物的含量和产量分别达到冷冻前的97%和95%.
Induction and Regulation of Crocus sativus L. Embryogenic Callus
藏红花胚性愈伤组织的发生及其调控

CHEN Shu-an,WANG Xiao-dong,ZHAO Bing,WANG Yu-chun,
陈书安
,王晓东,赵兵,王玉春

过程工程学报 , 2007,
Abstract: 为提高藏红花胚性愈伤组织的繁殖系数和出芽率,促进其生长和分化,以建立藏红花离体快繁体系,解决藏红花资源短缺问题,研究了藏红花胚性愈伤组织的发生及其调控.结果表明,获得的藏红花球茎1细胞系具有良好的胚性愈伤组织发生能力.胚性愈伤组织生长的优化条件为:在添加3.0mg/L 6-BA,0.25mg/L NAA和400mg/L CH的B5固体培养基上,22℃下全时暗培养25d,繁殖系数达到9g/g.胚性愈伤组织出芽的优化条件为:在添加3.0mg/L6-BA,0.25mg/LNAA和400mg/L CH的1/2 B5固体培养基上,在22℃及光照强度31.74μmol/(m2·s)条件下,每天光照10h,暗培养14h,培养45d出芽率达到44.7%,高于国外报道的20%.
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