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Search Results: 1 - 10 of 34952 matches for " YONG Qiang "
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Effect of melittin on apoptsis and necrosis of U2OS cells
CHEN Yong-Qiang
Zhong Xi Yi Jie He Xue Bao , 2004,
Abstract: Objective: To study the effect of melittin on apoptsis and necrosis of osteosarcoma cell line U2OS in vitro. Methods: Osteosarcoma cell line U2OS was treated with melittin. The growth and proliferation was observed by MTT assay and cell counting, and the necrosis was estimated by Trypan blue staining. The cell apoptsis, Fas and Apo2.7expression were detected by cytometer. Results: The data showed that melittin could inhibit the proliferation of U2OS dose-dependently at 16 and 64 mg/L. Cell apoptsis was detected by cytometer, when the cells were treated by 16 mg/L and 32 mg/L of melittin respectively, and the percentages of Fas and Apo2.7 positive cells were increased. Conclusion: Melittin inhibits the proliferation of osterosarcoma cell line through up-regulating Fas expression and inducing apoptsis.
Cost-Effective Strategy for the Investigation and Remediation of Polluted Soil Using Geostatistics and a Genetic Algorithm Approach  [PDF]
Yong-Qiang Cui, Minoru Yoneda, Yoko Shimada, Yasuto Matsui
Journal of Environmental Protection (JEP) , 2016, DOI: 10.4236/jep.2016.71010
Abstract: The geostatistical technique of Kriging has extensively been used for the investigation and delineation of soil heavy metal pollution. Kriging is rarely used in practical circumstances, however, because the parameter values are difficult to decide and relatively optimal locations for further sampling are difficult to find. In this study, we used large numbers of assumed actual polluted fields (AAPFs) randomly generated by unconditional simulation (US) to assess the adjusted total fee (ATF), an assessment standard developed for balancing the correct treatment rate (CTR) and total fee (TF), based on a traditional strategy of systematic (or uniform) grid sampling (SGS) and Kriging. We found that a strategy using both SGS and Kriging was more cost-effective than a strategy using only SGS. Next, we used a genetic algorithm (GA) approach to find optimal locations for the additional sampling. We found that the optimized locations for the additional sampling were at the joint districts of polluted areas and unpolluted areas, where abundant SGS data appeared near the threshold value. This strategy was less helpful, however, when the pollution of polluted fields showed no spatial correlation.
Self-Coexistence Strategy in Heterogeneous Cognitive Radio Networks  [PDF]
Qiang Zhang, Yong Zhang, Mei Song, Dawei Dang
Int'l J. of Communications, Network and System Sciences (IJCNS) , 2017, DOI: 10.4236/ijcns.2017.105B031
Different from general cognitive wireless networks, there is no centralized scheduling and management infrastructure among heterogeneous cognitive networks. Multiple cells may operate in the same vicinity resulting in unfair spectrum occupation time (when the cells belong to different industries) and degraded performance of the cellular networks. A distributed self-coexistence mechanism is necessary. In this paper, we take the self-coexistence of multi users in heterogeneous scenarios as the problem of spectrum allocation in non-cooperative mode. Hence we propose Fair Self-Coexistence Strategy (FSCS). In this strategy, not only the fairness of occupation time is considered, but also different competitive priority metric based on Quality of Service (QoS) is adopted. Each cognitive cell independently completes the spectrum allocation process, by use of sensing techniques and perceptual information about neighboring network cells. The simulation experiment results show that our spectrum allocation strategy guarantees the fairness among the heterogeneous secondary networks. And in the resource scarce environment, our strategy can effectively achieve the differentiation competition results.
Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate  [PDF]
Jun-Jun Zhu, Qiang Yong, Yong Xu, Shang-Xing Chen, Shi-Yuan Yu
Natural Science (NS) , 2009, DOI: 10.4236/ns.2009.11009
Abstract: Yeast Pichia stipitis CBS 5776 was developed through adaptation fermentation step by step in steam exploded corn stover prehydrolyzate because high concentration of weak acids and other inhibitors present in the prehydrolyzate could degrade the fermentability. However, the adaptability of Pichia stipitis CBS 5776 in the prehydrolyzate was so limited that steam strip-ping and overliming were applied to remove these inhibitors from it. Corn stover was steam exploded; the filtrate of steam exploded corn stover was hydrolyzed with dilute sulfuric acid, and then the acid hydrolyzate was detoxified and fermented by Pichia stipitis CBS 5776. Steam stripping could remove volatile com-pounds from the acid hydrolyzate and the fil-trate. At a steam stripping time of 120min, 81% acetic acid and 59% formic acid were removed from the acid hydrolyzate, 77% acetic acid and 45% formic acid were removed from the filtrate, while furfural was stripped off completely from the acid hydrolyzate and the filtrate. Overliming could reduce the contents of furfural and phe-nolics present in the acid hydrolyzate, however, sugars, especially pentoses, were also removed partially. It was necessary to detoxify the acid hydrolyzate in order to ferment the sugars to ethanol. Acid hydrolyzate detoxified with a combination of steam stripping for 120 min and overliming at pH11 and 60℃ for 90 min, its fer-mentability was significantly improved. Xylose was consumed nearly completely in 24h with an ethanol yield of 15.92g/l, 80.34% of theoretical.
Transcriptional regulatory programs underlying barley germination and regulatory functions of Gibberellin and abscisic acid
Yong-Qiang An, Li Lin
BMC Plant Biology , 2011, DOI: 10.1186/1471-2229-11-105
Abstract: The studies examined transcriptomes of barley representing six distinct and well characterized germination stages and revealed that the transcriptional regulatory program underlying barley germination was composed of early, late, and post-germination phases. Each phase was accompanied with transcriptional up-regulation of distinct biological pathways. Cell wall synthesis and regulatory components including transcription factors, signaling and post-translational modification components were specifically and transiently up-regulated in early germination phase while histone families and many metabolic pathways were up-regulated in late germination phase. Photosynthesis and seed reserve mobilization pathways were up-regulated in post-germination phase. However, stress related pathways and seed storage proteins were suppressed through the entire course of germination. A set of genes were transiently up-regulated within three hours of imbibition, and might play roles in initiating biological pathways involved in seed germination. However, highly abundant transcripts in dry barley and Arabidopsis seeds were significantly conserved. Comparison with transcriptomes of barley aleurone in response to GA and ABA identified three sets of germination responsive genes that were regulated coordinately by GA, antagonistically by ABA, and coordinately by GA but antagonistically by ABA. Major CHO metabolism, cell wall degradation and protein degradation pathways were up-regulated by both GA and seed germination. Those genes and metabolic pathways are likely to be important parts of transcriptional regulatory networks underlying GA and ABA regulation of seed germination and seedling growth.The studies developed a model depicting transcriptional regulatory programs underlying barley germination and GA and ABA regulation of germination at gene, pathway and systems levels, and established a standard transcriptome reference for further integration with various -omics and biological data to
Effects of rat serum containing Chinese herbal medicine Sangen Decoction on osteoclastogenesis and bone resorption of osteoclasts induced by polymethylmethacrylate particles
Shu-qiang Wang,Yong-qiang Chen
Zhong Xi Yi Jie He Xue Bao , 2011,
Abstract: Objective: To investigate the effects of Sangen Decoction, a compound Chinese herbal medicine, on osteoclastogenesis and bone resorption function of osteoclasts induced by polymethylmethacrylate particles in vitro.Methods: Macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL) were used to induce differentiation of bone marrow-derived macrophages (BMMs) towards osteoclasts. BMMs and polymethylmethacrylate particles with ratio of 1︰3 were added to the 24-well plate and 96-well plate with bone slices respectively. A total of 50 male SD rats were divided into 5 groups randomly with each group containing 10 rats. After being treated with different drugs, serum samples of rats in each group were extracted, i.e., the blank serum, Western medicine (ibandronate) serum and high-, medium-, and low-dose Sangen Decoction serum and were added to the medium respectively. The tartrate-resistant acid phosphatase (TRAP) staining was used to identify the differentiation of BMMs and for counting of osteoclasts. Area of lacuna induced by osteoclast bone resorption on the bone slices was measured by computer image processing.Results: Numbers of osteoclasts of treatment groups were less than that of blank group by TRAP staining (P<0.05); numbers of osteoclasts of positive control group and high-dose Sangen Decoction group were much lower than those of medium- and low-dose Sangen Decoction groups (P<0.05), and no difference was found between Western medicine group and high-dose Sangen Decoction group (P>0.05). In bone resorption assay, area of lacuna of blank group was larger than those of treatment groups (P<0.05); areas of lacuna of Western medicine group and high-dose Sangen Decoction group were much smaller than those of medium- and low-dose Sangen Decoction groups (P<0.05), and no difference was found between Western medicine group and high-dose Sangen Decoction group (P>0.05).Conclusion: Sangen Decoction can inhibit osteoclastogenesis induced by polymethylmethacrylate particles as well as bone resorption function of osteoclasts.
Serum homocysteine concentrations of Chinese intellectuals and the influential factors concerned  [PDF]
Yue Hou, Yan Hong, Wei-Qiang Chen, Dong-Lan Wang, Yi-Yong Cheng
Health (Health) , 2009, DOI: 10.4236/health.2009.12014
Abstract: Objective: To observe the concentration of se-rum homocysteine in intellectuals and the re-lated influential factors. Methods: The concen-trations serum homocysteine and saliva cortisol were measured in 138 intellectuals from three cities, Tianjin, Guangzhou and Chengdu in China. All the subjects had senior titles of tech-nical post, aged 40-69 years. Results: The mean value of serum homocysteine concentration in intellectuals was 20.6±0.8μmol/L, higher than the reference value. With the increase of cortisol levels the homocysteine concentrations rise (P <0.05). The mean value of homocysteine con-centration was highest in 40-49 years old group. Men had higher homocysteine level than women in this investigation. According to the mean value of homocysteine concentration among different cities, Tianjin was highest, Chengdu medium, Guangzhou lowest. Conclusion: The serum homocysteine concentration of intellec-tuals is higher than the reference value. The stress level, gender and resident cities might contribute to the differences in serum homo-cysteine concentration in Chinese intellectuals.
Melamine Nitrate: A Novel and Efficient Reagent for Regioselective Nitration of Phenols
Yong-qiang Chen,Hong Jiang
Organic Chemistry International , 2011, DOI: 10.1155/2011/753142
Abstract: Melamine nitrate (MN) as a novel nitration reagent was easily prepared. Regioselective nitration of phenols to their corresponding o-nitrophenols occurred using MN with p-toluenesulfonic acid as catalyst in good to excellent yields. A distinct advantage of this method is the easy separation of products by simple filtration. 1. Introduction Nitration of aromatic compounds is one of the most important and widely studied reactions and industrial processes. The typical nitration procedure requires use of mixed acids such as concentrated nitric acid and sulfuric acid. Nitrophenols are important intermediates for the manufacture of drugs and pharmaceuticals [1]. But phenols are highly reactive; therefore the nitration of phenols by mixed acids is always associated with the formation of dinitro compounds, oxidized products, and unspecified resinous materials. So a lot of mild nitration processes for phenols have been developed to overcome these shortcomings. Especially, in recent years, various nitrate salts for phenols have been reported [2–10]. However, some of the nitrating reagents are poorly regioselective and uneconomical. Considering these concerns, there is still a good scope for research towards finding economic, mild reagents for regioselective nitration of phenols. Melamine is a widely used fire retarder in polymers. The amino groups of melamine are stable to oxidation condition such as H2O2, in which it can form stable adduct with H2O2 [11, 12]. This inspired us to think that melamine may be stable in oxidative acid such as nitric acid. So in this paper we prepared the melamine nitric acid complex (MN) (Figure 1) and used it as nitration reagent. Figure 1 Herein we report this efficient and facile nitration procedure for phenols using MN (Scheme 1). The preparation of melaminium nitrate is simple by the direct reaction of melamine with nitric acid at room temperature. Scheme 1 2. Results and Discussions Table 1 summarized the results of nitration of phenol by MN in different solvents such as CCl4, CH2Cl2, and CHCl3; the highest yield of 2-nitrophenol was isolated in acetone (Table 1, entry 1). So in the following reactions, acetone was used as solvent. Different other catalytic acids like, acetic acid, benzoic acid, and sulfuric acid were also tested but they either gave trace product (in acetic acid or benzoic acid) or very complicated products (in sulfuric acid); so p-toluenesulfonic acid was chosen as catalytic acid. Table 1: Nitration of phenol with different solvents by MN. The results of nitration reactions of other phenolic compounds by
Progress in artificial control system for gene expression
Yong Zhang,Nanming Zhao,Qiang Liu
Chinese Science Bulletin , 2000, DOI: 10.1007/BF02886189
Abstract: Along with the increasingly wide application of transgenic techniques, new stricter criteria have been raised for controlling the expression of exogenous genes. For these demands, a series of artificial control systems for gene expression have been developed and testified in recent years, which can control exogenous genes expression in exact time and certain level by administration of a specific drug or hormone. The successful construction of these systems offers a practicable method to control precise expression of exogenous gene in organisms, and raises the feasibility of wide application of gene therapy.
Plant protein kinase genes induced by drought, high salt and cold stresses
Qiang Liu,Yong Zhang,Shouyi Chen
Chinese Science Bulletin , 2000, DOI: 10.1007/BF02886068
Abstract: Drought, high salt and cold are three different kinds of environment stresses that severely influence the growth, development and productivity of crops. They all decrease the water state of plant cells, and consequently result in the harm of plant from water deficit. Several genes encoding protein kinases and induced by drought, high salt and low temperature have been isolated fromArabidopsis. These protein kinases include receptor protein kinase (RPK), MAP kinases, ribosomal-protein kinases and transcription-regulation protein kinase. The expression features of these genes and the regulatory roles of these protein kinases in stress response and signal transduction are discussed.
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