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Search Results: 1 - 10 of 53950 matches for " YING Xue-ping "
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Structural Characteristics of Normal and Abnormal Embryos Attachment of Eriocheir sinensis
中华绒螯蟹正常附着胚胎与流产胚胎的结构特点

YING Xue-ping,
应雪萍

动物学研究 , 2005,
Abstract: The structural features of normal and abnormal embryo attachment systems of the Chinese mitten crab Eriocheir sinensis were investigated by scanning electron microscopy. The results showed that the volume of in cubation fluid inthe brooding chamber increased 15 minutes after egg-laying, immersing the embryos in the incubation fluid and thus the egg envelop became very sticky immediately. The egg stalk was largely formed within 30 minutes and completed within 80 to 140 minutes after egg-laying, so that the egg envelop became strongly adherent to the ovigerous hairs. In normal embryos, the egg stalks were highly twisted and the embryos were attached to the ovigerous hair by the egg stalk or funiculus. Many villiform were found on egg stalks and a lot of mucus was also found on the ovigerous seta. In abnormal egg stalks, villiform were never found, though numerous epibionts were attached to the embryo surface and egg stalk. The mucus of ovigerous hair and the villiform of egg stalks may contribute to egg attachment. Because of the epibionts the structure of egg investment coat egg stalk and funiculus were changed, enhancing the attrition between embryos and making embryo respiration difficult. The death and loss of embryos occur because of the above reasons.
Ultrastructure and function of the hepatopancreas of Bullacta exarata
泥螺肝胰腺细胞的超微结构和功能

YING Xue-ping,
应雪萍

海洋科学 , 2007,
Abstract: The ultrastructure of the hepatopancreas of Bullacta exarata was studied through electronic microscopy.The result shows that the hepatopancreas of B.exarata are composed of four types of cells in the wall of tubules:the embryonic cell(E-cell),resorptive cell(R-cell),fibrillar cell(F-cell),and blister cell(B-cell).B-cell contains numerous digestive vesicle.R-cell.demonstrates a marked increase in lipid droplet size and number over F-cell.The F-cell has more rough sndoplasmic reticulum,mitochondria and free ribosomes.E-cell are polyhedral.The cytoplasm contains relatively sparse rough endoplasmic reticulum,small mitochondria.The ultrastructures of these cells were given and their functions were discussed.The differentiation sequences of hepatopancreas were suggested in this paper.
Ultrastructural Studies on Spermatogenesis of Bullacta exarata (Philippi)
泥螺精子发生的超微结构研究

YING Xue-ping,JIANG Nai-cheng,YANG Wan-xi,
应雪萍
,姜乃澄

动物学研究 , 2002,
Abstract: The ultrastructure of spermatogenesis of Bullacta exarata was studied by using of transmission electron microscopy.The results show that there is a series of considerable changes in morphology and structure during the spermatogenesis.These changes include gradual nuclear elongation,chromatin condensation,formation of acrosome,development and fusion of mitochondria,cytoplasmic sloughing and flagellar formation.Acrosome is formed from proacrosomal vesicle fused with vesicles secreted by Golgi apparatus.Axoneme is formed from centriole.Spermatid differentiation can be divided into three stages.During the differentiation,the morphology and structure of nucleus and chromation are different from other Mollusks.The nucleus of early spermatid is oval-shaped and comprises heterogeneous aggregations of flocculent granular chromatin.In the middle stage,it is kidney shaped and its chromatin condenses to finely fibrils-shaped.As the spermatid matures,the nucleus elongates in the anterior-posterior axis,and the chromatin condenses to long fibrils and then condenses to high and even electron-density till the formation of spermatozoon nucleus.At the same time,the nucleus transforms from cylindric shape to long cylindric shape.Significance of nucleus and cell organelle as taxonomic character in the spermatogenesis of B.exarata is roughly discussed.
Ultrastructure of the Spermatozoon of the Northern Grass Lizard (Takydromus septentrionalis) with Comments on the Variability of Sperm Morphology Among Lizard Taxa
北草蜥精子的超微结构——兼评不同类群蜥蜴精子形态的差异

ZHANG Yong-pu,YING Xue-ping,JI Xiang,
张永普
,应雪萍,计翔

动物学研究 , 2005,
Abstract: We studied the ultrastructure of the spermatozoon of northern grass lizards ( Takydromus septentrionalis , n = 5) collected in April 2003 from a population in Wenzhou, Zhejiang, eastern China. The mature spermatozoa are characterized by: A circular acrosome; an acrosomal vesicle divided into cortex and medulla; the unilateral ridge of the acrosome vesicle divided into cortex and medulla, the electron-lucent zone between them; a prenuclear perforatorium, absence of the perforatorium base plate; presence of the subacrosomal space; an elongated nucleus, absence of a endonuclear canal; absence of epinuclear lucent zone; rounded nuclear shoulders. A bilateral stratified laminar structure within the neck region. A short midpiece; absence of multilaminar membranes; in longitudinal section, two tiers of mitochondria, in transverse section, six mitochondria with linear cristae; the presence of two dense body groups, the ring structure consisting of the complete ring; the arrangement pattern of the ring structure and mitochondria in rsl/mil and rs2/mi2; a fibrous sheath in the midpiece; presence of an annulus. A thin granular zone of cytoplasm at the anterior portion of the principal piece; the enlarged fiber 3 and fiber 8 disappearing at the anterior portion of the principal piece; the axoneme complex having an usual 9 + 2 pattern. Within lacertid lizards, sperms differ among species in the number of mitochondria, but in all species studied so far, there are two groups of dense bodies. However, lizards of different taxa differ, in various degrees, in such sperm morphological traits as acrosome vesicle, subacrosomal space, epinuclear lucent zone, perforatorium base plate, nuclear shoulder and number and arrangement of mitochondria and dense bodies. These differences suggest that sperm morphology provides additional information which one may use to study the phylogeny of lizards.
Morphological variation in three geographical populations of Estellarca olivacea
橄榄蚶三个地理群体的形态差异分析

ZHANG Yong-pu,YING Xue-ping,WANG Tie-gan,CHAI Xue-liang,
张永普
,应雪萍,王铁杆,柴雪良

海洋科学 , 2009,
Abstract: Based on nine morphological characters of populations of Estellarca olivacea from Zhejiang Lingkun, Zhejiang Sanmen and Jiangsu Tongzhou, the morphological variations of three geographical populations were studied by means of multivariate morphometrics. The results of cluster analysis and principal component analysis revealed that the morphological characters of E. olivacea from Zhejiang Sanmen population and Zhejiang Lingkun population were similar to each other but were much more different from the character of Jiangsu Tongzhou population. In the principal component analysis, two principal components were constructed. The contributory ratios of the two principal components were 47.73% and 13.56% respectively and the cumulative contributory ratio was 61.29%. The shell protrusion or flat, shell thick and fore-and-aft shape determined the morphological variations of different geographical populations of E. olivacea, which were related with the water temperature and substrate.
EFFECTS OF DIETHYLSTILBESTROL EXPOSURE IN EARLY LIFE STAGE ON DEVELOPMENT AND REPRODUCTION IN RARE MINNOW, GOBIOCYPRIS RARUS
稀有鮈鲫生命早期的己烯雌酚暴露对生长发育与繁殖的影响

ZHONG Xue-Ping,XU Ying,LIANG Yong,LIAO Tao,WANG Jian-Wei,
钟雪萍
,徐盈,梁勇,廖涛,王剑伟

水生生物学报 , 2005,
Abstract: 研究了己烯雌酚(DES)对稀有鲫(Gobiocypris rarus)生命早期暴露的影响。经10μg/L和100μg/L己烯雌酚暴露26d后,稀有鲫死亡率升高,生长发育迟缓,鱼体内卵黄蛋白原(VTG)的诱导明显。经过一段时间清水养殖后,暴露组中雌鱼比例增加,雌雄鱼生长较对照组有显著变化。雌鱼性腺发育及产卵量与对照组相比虽无显著差异,但暴露组成鱼所繁育后代与对照组相比受精率、孵化率显著下降,死亡率、畸形率明显上升。这些结果说明己烯雌酚生命早期暴露影响稀有鲫的生长发育及生殖,稀有鲫生命早期暴露实验可以用于评价水生态系统中内分泌干扰物的生态影响。
Domestication of the high-sugar-tolerant Mortierella alpina on arachidonic acid (ARA) production
花生四烯酸产生菌高山被孢霉的高糖驯化研究

ZENG Si-Yu,LING Xue-Ping,ZHANG Chang-Jie,LU Ying-Hua,
曾思钰
,凌雪萍,张长杰,卢英华

微生物学通报 , 2012,
Abstract: 【目的】提高花生四烯酸(Arachidonic acid, ARA)产量, 克服ARA产生菌高山被孢霉(Mortierella alpina)在长期的保存及使用过程中易受到外界条件影响发生退化, 从而导致菌种耗糖量降低、影响菌种摄入营养的能力和不利于工业化生产的缺点。【方法】首先采用固体培养基驯化, 将菌种逐级涂布于梯度高糖PDA平板(含糖量分别为2%、5%、7%、10%和15%)培养, 挑选经固体驯化后能耐受10%高糖浓度平板的菌种, 转接到两种含不同氮源的梯度高糖(含糖量分别为3%、4%、5%和6%)液体培养基中进行驯化, 最后对驯化后的菌种进行2 L发酵罐放大实验。【结果】当培养基中以酵母粉为氮源时, 驯化后菌体的最高耗糖量由3 g/(L·d)提高到12 g/(L·d); 当培养基中以玉米浆为氮源时, 驯化后菌体的最高耗糖量由7 g/(L·d)提高到12 g/(L·d)。摇瓶驯化实验结果表明以玉米浆为氮源驯化的菌种发酵效果较好, 发酵罐实验结果显示菌体生物量为50 g/L, 总油脂为18 g/L, 目的产物ARA产量为8 g/L。相比未驯化之前的发酵结果, 生物量和总油脂含量提高了近3倍, ARA产量提高了近4倍。【结论】经过高糖驯化, 菌种的耗糖能力得到提高, 生物量、总油脂及ARA的产量也都有所增加, 从而可以使菌种在保存和使用过程中不易退化, 保持稳定。
Lifelong Vocational Education for Sustainable Development
面向可持续发展的终身职业教育

WU Xue-ping,
吴雪萍

浙江大学学报(人文社会科学版) , 2007,
Abstract: Promoting the employment of the work force and the development of human resources is an important issue that needs to be resolved in China.It is also crucial to building a well-off society.Premier Wen Jiabao emphasizes the importance of vocational education in a speech delivered at the National Conference on Vocational Education.China's economic development and employment is facing two great changes: both employment of the work force and optimization of the industrial structure are in need of skilled workers.Therefore,China will have to devote great effort to developing vocational education.Developing lifelong vocational education plays an important role in promoting the development of human resources,the employment of labor force,the sustainable development and enhancing the country's competitive power.The idea of lifelong vocational education comes from the idea of lifelong education.It is a new perception of vocational education.It breaks the restrictions of time and space in the narrow sense of vocational education.According to the idea of lifelong vocational education,vocational education should run throughout one's life to provide help for one's whole career development and should be an integration of all kinds of vocational education in different places.Lifelong education has the features of continuation,integration,flexibility,openness and change.Developing lifelong vocational education is a complex work that needs social participation.The basic strategies for developing lifelong vocational education include law assurance,policy control,flexible fund raising,social participation and encouragement for lifelong learning.In law assurance,regulations for lifelong vocational education may be made according to the Vocational Education Act of the P.R.China and local acts may be enacted.In policy-making,there are many aspects as establishing and coordinating the institutions of lifelong vocational education,strengthening the functions of educational departments,building opening schools and providing learning holidays.In flexible fund raising,funding system that combines special grants from governments,funds from industries, funds from society and individual learning accounts may be established.In social participation,platform for participation can be set up and activities for creating learning organizations can be launched.The main forms of lifelong learning encouragement include providing opportunities for development,spiritual awards,economic benefits and qualification accreditation.
Smoothness and high energy asymptotics of the spectral shift function in many-body scattering
Andras Vasy,Xue-Ping Wang
Physics , 2001,
Abstract: Let H=\Delta+\sum_{#a=2} V_a be a 3-body Hamiltonian, H_a the subsystem Hamiltonians, \Delta the positive Laplacian of the Euclidean metric on X_0=R^n, V_a real-valued. Buslaev and Merkurev have shown that, if the pair potentials decay sufficiently fast, for \phi smooth and compactly supported, the operator \phi(H)-\phi(H_0)-\sum_{#a=2}(\phi(H_a)-\phi(H_0)) is trace class. Hence, one can define a modified spectral shift function \sigma, as a distribution on R, by taking its trace. In this paper we show that if V_a are Schwartz, then \sigma is in fact smooth away from the thresholds, and obtain its high energy asymptotics. In addition, we generalize this result to N-body scattering, N arbitrary.
Determination of gamma-aminobutyric acid and seventeen free amino acids in Pinus massoniana Needles by reversed-phase high performance
柱前衍生反相高效液相色谱法测定马尾松(Pinus massoniana )针叶中γ-氨基丁酸和17种游离氨基酸

DENG Wen-Hong,SHEN Ying-Bai,LI Zhen-Yu,HANG Shu-Jing,LIU Xue-Ping,
邓文红
,沈应柏,李镇宇,张淑静,刘雪萍

生态学报 , 2008,
Abstract: 建立了自动在线柱前衍生反相高效液相色谱法同时测定γ-氨基丁酸(GABA)和17种游离氨基酸含量的方法.以邻苯二甲醛-9-芴基甲基氯甲酸酯(OPA-FMOC)为衍生试剂进行衍生,Agilent Hypersil AA-ODS-C18色谱柱分离,梯度洗脱,二极管阵列检测器检测,在19min内分离测定了马尾松苗木针叶中GABA 和17种游离氨基酸的含量.该方法测定氨基酸的回收率高于90.1%,精密度和重现性均较好(相对标准偏差为0.21%~2.81%),经测定,发现马尾松被马尾松毛虫取食后,所测18种氨基酸总量明显降低,从418.3μg · g-1降低到310.4μg · g-1鲜重.
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