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Search Results: 1 - 10 of 40924 matches for " YIN You-Ping "
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Joint Statement of Establishing Chinese Clinical Trial Registration and Publishing System
You-Ping LI
Zhong Xi Yi Jie He Xue Bao , 2006,
Abstract:
Cloning and expression analysis of an hydrophobin gene Nrhyd in Nomuraea rileyi
莱氏野村菌疏水蛋白基因Nrhyd的克隆及其表达特征分析

HUANG Shan,WANG Zhong-Kang,CHEN Huan,WANG Meng,YIN You-Ping,
黄姗
,王中康,陈环,王萌,殷幼平

菌物学报 , 2012,
Abstract: The full-length cDNA of a hydrophobin gene Nrhyd gene was cloned from the insect pathogenic fungus Nomuraea rileyi using SMART RACE RT-PCR. The expression of Nrhyd gene by N. rileyi under different culture conditions was analyzed using Realtime-PCR, and the effects of different culture conditions on expression of Nrhyd gene on mRNA level were explored. Sequence analysis shows that the cDNA contains an open reading frame of 339bp which encoding a polypeptide with 111 amino acids. The precursor protein has a molecular mass of 10.6 and a calculated pI of 6.19. The RT-qPCR results showed that the expression of Nrhyd gene was suppressed under liquid culture condition. While under solid agar culture condition, the expression level of Nrhyd gene increased along with the formation of conidia and reached the highest level at day 8 of post-inoculation, and then decreased gradually. It is inferred therefore that the Nrhyd gene might play an important role in the formation of conidia in N. rileyi. Phylogenetics analysis of Nrhyd gene with other fungal hydrophobin indicates the gene is most closely related to the homologues from Metarhizium spp.
Adjusting-controlling on gassing and energy for high power excimer laser based on intelligence
基于智能的大功率准分子激光器配气与能量的调控

FAN You-ping,HUANG Xi-yue,YU Yin-shan,
樊友平
,黄席樾,余吟山

控制理论与应用 , 2002,
Abstract: The system, which adopts gas and energy as controlling measure, is a nonlinear and variable delay time system. To confirm such a system's accurate mathematical model and optimum answer is very difficult. A new method is presented to form a set of fuzzy control rules by means of human operator's control actions and proposes an intelligence control method based on the knowledge/reasoning regulation. It is guided with the knowledge base ,to realize fast reaction and high precision. This device has the features of easy to operate, flexible control and small error. Measurements show that the device is stable and practicable.
Isolation of facultative anaerobic entophytic bacterial companioned Ca Las infected citrus tissues and evaluation of dominant bacterial populations
亚洲韧皮杆菌兼性厌氧型伴生细菌鉴定及优势菌群分析

LI Yan-Fang,YIN You-Ping,WANG Yu-Xi,LI Jia,CHEN Shi-Wei,WANG Zhong-Kang,
李颜方
,殷幼平,王玉玺,李佳,陈世伟,王中康

微生物学通报 , 2011,
Abstract: To analysis the entophytic bacterial diversity of citrus and find the companion bacteria populations associated with Huanglongbing pathogen-infected and healthy citrus plant tissues for decipher the co-cultivation of HLB pathogen, we selected varied parts of citrus tissues collected from different loca-tions of citrus planted area. The facultative anaerobic entophytic bacteria were isolated and purified based on bacterial morphology, physiology, biochemistry characteristics and the molecular method of PCR-DGGE (Denaturing gradient gel electrophoresis) analysis based on the sequence of 16S rRNA V6-V8 fragment gene. By the directional isolation of the facultative anaerobic entophytic bacteria and 16S rDNA amplification, total 12 genera of bacteria were identified from 19 cultivable bacterial popula-tions. The dominant bacterial population in infected citrus plants were Curtobacterium sp. (IF: 29.07%), Bacillus sp. (IF: 23.12%), Microbacterium sp. (IF: 21.09%) while in healthy citrus tissues belonged to Bacillus sp. (IF: 21.03%), Planococcus sp. (IF: 20.69%), Pseudomonas sp. (IF: 17.44%). From 50 target bands obtainded by the DGGE approach, 9 genera of cultivable bacteria were recognized. The dominant bacterium population belonged to Serratia sp. (IF: 28%) and Pantoea sp. (IF: 14%) followed by it. Can-didatus Liberibacter asiaticus was only found in tangerine pith of deformed orange fruit, which sug-gested that the content (>1%) of Huanglongbing was more in diseased fruits and other tissues of citrus had low abundance percentage. The density and species of entophytic bacteria were also observed in re-markable difference between infected and healthy citrus plant from the PCR-DGGE profiles.
Isolation and characterization of endophytic bacteria from huanglongbing’s hidden host plant- Murraya paniculata
柑橘黄龙病隐症寄主九里香内生细菌分离及功能鉴定

YIN You-ping,LI Ji,LIN Ya-yu,CHEN Shi-wei,WANG Zhong-kang,
殷幼平
,李佳,林亚玉,陈世伟,王中康

微生物学通报 , 2012,
Abstract: 【目的】分析黄龙病高发区的九里香植株叶及茎干中内生细菌,为寻找具有抗柑橘黄龙病的内生细菌奠定基础。【方法】利用平板培养法及基于16S rDNA的限制性酶切长度多态性(Restriction fragment length polymorphism,RFLP)序列分子鉴定法,对九里香植物内生细菌进行多样性分析。【结果】在兼性厌氧的生长环境下,从九里香植株中分离获得可培养内生细菌26株,分属于9个细菌属的14个种,其中肠杆菌属(Enterobacter sp.)(IF=19.23%)、芽胞杆菌属(Bacillus sp.)(IF=38.46%)为九里香可培养内生细菌的优势菌属。茎干中内生细菌丰度高于叶中内生菌丰度。建立了九里香内生细菌16S rDNA文库,对文库质量检测显示,该克隆文库的覆盖度(Coverage C)为94.97%,结合Rarefaction曲线分析,表明所构建的克隆文库是相对充分的。对文库中179个阳性克隆进行HaeШ、MspⅠ、RsaⅠ3种限制性内切酶分析,得到20个不同的操作分类单元(Operational taxonomic units,OTUs),其中沙雷氏菌属(Serratia sp.)为九里香植株中内生细菌的绝对优势菌属。测定了14株内生细菌的功能,其中9株菌能产生吲哚-3-乙酸(IAA);具有抗生素(phlD)合成能力的内生细菌有4株;结合nifH和NFb固氮培养基确定有3株内生细菌具有固氮能力;1株内生细菌具有ACC脱氨酶合成能力;8株内生细菌具有铁细胞合成能力;3株内生细菌具有淀粉水解能力;2株内生细菌显示强阳性的蛋白酶合成能力,4株内生细菌具有以上4种功能。【结论】九里香植株中内生细菌具有丰富的多样性,并且可能对九里香植株生长发育及抗生物和非生物胁迫有着重要的生理功能。
Isolation and identification of endophytic fungi with antimicrobial activity from Fagopyrum dibotrys and Fagopyrum tataricum
金荞麦和苦荞麦抗菌活性内生真菌的筛选及鉴定

ZHANG Chuan-Bo,CHEN Rong-Lin,YIN You-Ping,WANG Zhong-Kang,
张传博
,陈荣林,殷幼平,王中康

微生物学通报 , 2011,
Abstract: A total of 62 endophytic fungi were isolated from the plant tissues (root, stem, leaf and flower) of medicinal plants Fagopyrum dibotrys and Fagopyrum tataricum. The antimicrobial activity from isolated endophytic fungi were detected using agar diffusion method with Staphylococcus aureus, Es-cherichia coli, Bacillus subtilis CMCC (B) 63501], Fusarium graminearum, Fusarium oxysporum f. sp. cucumerinum and Pythium aphanidermatum as indicator microorganisms. The results showed that the ethanol extracts of three isolated entophytic fungi strain KQH-01, KQH-02 and JQY-1 had relatively strong antibiotic activity to some indicator microorganisms. By using morphological and molecular identification, strain KQH-01was determined to belong to the genus Xylaria sp., and strains KQH-02 and JQY-1 were identified as Chaetomium globosum and Botryosphaeria dothidea respectively.
Cloning and characterization of the neutral trehalase gene in Metarhizium anisopliae CQMa102
金龟子绿僵菌中性海藻糖酶基因的克隆及其表达特性分析

HU Zong-Li,WANG Zhong-Kang,PENG Guo-Xiong,YIN You-Ping,XIA Yu-Xian,CAI Shao-xi,
胡宗利
,王中康,彭国雄,殷幼平,夏玉先

微生物学报 , 2005,
Abstract: 根据中性海藻糖酶NTL基因的同源序列设计引物,PCR扩增出杀蝗专一菌株———金龟子绿僵菌CQMa102NTL基因片段,利用5′_RACE和3′_RACE扩增出NTLcDNA的5′和3′端序列,经拼接得到CQMa102NTL基因cDNA全长。根据其全长cDNA序列,设计引物PCR扩增出CQMa102NTL的完整基因。为了解该基因的上游调控信息,采用PanhandlePolymeraseChainReactionAmplification方法扩增其上游序列。序列分析表明,CQMa102NTL全长DNA3484bp,cDNA全长2385bp,编码737个氨基酸的蛋白,推测蛋白分子量为83.1kD;含有3个内含子,包含一个依赖于cAMP的磷酸化作用位点(RRGS)和一个钙附着位点(DTDGNMQITIED);上游序列含有一个压力反应元件(CCCCT);与金龟子绿僵菌广谱性菌株ME1NTL的核苷酸序列和氨基酸序列分别具有93%和99%同源性,由此确定该序列为金龟子绿僵菌中性海藻糖酶基因序列。Southern杂交表明,NTL基因在CQMa102基因组中为单拷贝。Northern杂交表明,NTL基因转录出约2.5kb的mRNA单带,在液体培养条件下,对数生长前期表达水平最高,对数生长后期降到最低,进入稳定生长期后表达水平又有所提高。金龟子绿僵菌CQMa102中性海藻糖酶基因DNA全长和cDNA全长登录GenBank,登录号分别为:AY557613,AY557612。
Selection of differential DNA fragment of Tilletia controversa Kühn and establishment of molecular detection approach
小麦矮腥黑穗菌差异片段筛选与分子检测体系的建立

NIAN Si-ji,YIN You-ping,YUAN Qing,XIA Yu-xian,WANG Zhong-kang,
年四季
,殷幼平,袁青,夏玉先,王中康

微生物学报 , 2007,
Abstract: A reliable and simple polymerase chain reaction method for TCK pathogen was established firstly. A 1322bp unique fragment of TCK was amplified and identified by the technique of semi-specific random amplified polymorphism (RM-PCR). Two pairs of species-specific primers CQUK_2/ CQUK_3 and CQUK_4/ CQUK_5 were designed according to the unique fragment of TCK. The first pair primers were capable to stably amplify target DNA band of 747bp from chromosomal DNA of 18 strains of TCK isolates without any DNA bands obtained from 29 strains of TCT. The second pair primers could produce a 200bp target DNA band stably, while no band was amplified from teliospore or mycelium DNA of TCT of strains. Tilletia genus primers were used as internal control of molecular detection system, which can detect whether the PCR inhibitors exist in testing sample or avoid pseudo-negative and pseudo-positive of PCR reaction. The molecular detection approach could rapidly, accurately detect and identify the DNA of teliospore or mycelium of TCK from wheat tissues.
High efficient expression and bio-activity assay of recombinant antibody for citrus bacterial canker disease
柑桔溃疡病菌重组单链抗体的高效表达及活性检测

CHEN Gang,YIN You-ping,YUAN Qing,XIA Yu-xian,WANG Zhong-kang,
陈刚
,殷幼平,袁青,夏玉先,王中康

微生物学报 , 2007,
Abstract: Citrus bacterial canker disease caused by the gram negative bacterium Xanthomonas axonopodis pv. citri (XAC) is a severe bacterial disease of most commercial citrus species and cultivars around the world. Single chain variable fragment (ScFv) is artificial construction small molecular antibody produced by genetic engineering which may be used to identify target pathogens and prevent plant diseases including XAC. To express ScFv against XAC and obtain functional ScFv, single-chain antibody fragments (ScFv 95) selected by ribosome display was amplified using an assembly of polymerase chain reaction (PCR), and a recombined plasmid pET30a( )-XAC-ScFv was constructed by inserting the single chain Fv gene into bacterial expression vector pET30a( ). PET30a( )-XAC-ScFv was transformed into Escherichia coli BL21 (DE3) and expressed which was induced by IPTG. Products were purified though Ni-NTA His Bind resin. The collected antibodies were refolded by gel filtration chromatography, and activity assaying process was done. The results showed that ScFv recombined antibody of XAC with a molecular of 32kDa was expressed successfully as inclusion bodies and the functional ScFv was obtained through purification and renaturation. Meanwhile, the Biacore analysis indicates that XAC-ScFv-95 showed significant affinity to LPS of Xac, which paves a new way for immunization diagnosis and exploration of integrated control of citrus bacterial canker disease.
Construction of Two Standard Molecules for Detection of Tilletia indica Mitra and Tilletia walkeri Castl.
小麦印度腥黑穗病菌和黑麦草腥黑粉菌检测标准分子构建

LI Xiao-Jiao,WANG Zhong-Kang,ZHANG Gui-Ming,CHEN Zhi-Nan,YIN You-Ping,CHENG Ying-Hui,WANG Ying,XIANG Cai-Yu,MIU Jian-Kun,
李小焦
,王中康,章桂明,陈枝楠,殷幼平,程颖慧,王颖,向才玉,缪建锟

微生物学通报 , 2010,
Abstract: Two standard molecules were constructed by molecular clone technology of Tilletia indica Mitra and Tilletia walkeri Castl.. The standard molecule for Tilletia indica Mitra contained two DNA fragments, one about 2297 bp DNA sequence from mitochondrial DNA, the other ITS sequence 710 bp. The standard molecule for Tilletia walkeri Castl. contained two DNA fragments, one about 2.3 kb DNA sequence from mitochondrial DNA, the other ITS sequence 710 bp. In this research, the two standard molecules were detected, the results show good specificity, homogeneity and stability which can satisfy the need of molecular detection of Tilletia indica Mitra and Tilletia walkeri Castl..
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