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Search Results: 1 - 10 of 11870 matches for " YIN Guangxiao "
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The Application of NdFeB in the Magnetic Force Actuator
DONG Enyuan,YIN Guangxiao,WANG Yongxing,DUAN Xiongying
TELKOMNIKA : Indonesian Journal of Electrical Engineering , 2012, DOI: 10.11591/telkomnika.v10i6.1410
Abstract: In this paper, NdFeB is used to design a new type of magnetic force actuator (MFA) with simple structure and high reliability. The permanent magnets are fixed on the static iron-core to generate a magnetic field, while the movable part locates within the magnetic field. It can drive the arc extinguishing unit powered by the Lorentz force, and this can be applied to the operation of the long-stroke high voltage circuit breaker (HVCB). At the open and closed position, the PMs generate holding force for the moving iron-core to keep the static state. Then, the finite element method(FEM) and prototype test are adopted to study the properties of PM and characteristics of the actuator. The simulation concludes that the material type and structure size of PM, end cap material and processing deviation of the actuator will impact the static characteristic of the actuator. The results of the test on prototype show that MFA using NdFeB can achieve the high power output, which is conductive for electronic control as well as the displacement tracking. Due to its stable performance, NdFeB is reliable in the running of the magnetic force actuator.
Oxidization Resistance in Vivo for Raspberry Flavone  [PDF]
Jinxu Sun, Huixia Zhu, Guangxiao Dong
Engineering (ENG) , 2013, DOI: 10.4236/eng.2013.510B093

The experiments on the effect of oxidization resistancein vivoforraspberryflavone extract manifests that the extract ofraspberryflavone could effectively increase CAT, SOD and GSH-Px enzyme activity in rats’ liver, kidney, blood and skeletal muscle and decrease decomposition product of lipid peroxidization, with a significant antioxidant effect.

The Exploitation and the Development Perspectives of New Environmental Foliage Fiber
Guangxiao Wang,Yi Zhang
Journal of Sustainable Development , 2009, DOI: 10.5539/jsd.v2n2p187
Abstract: In this paper, the author reviewed the development of the natural fabrics, expounding the actuality of the exploitation on new type environmental natural fabrics, analyzing the production and the traits of the natural fabrics and the chemical fiber, and pointed out the significance of empoldering new environmental foliage fibers to meet the demand of textile machining. The author introduced several kinds of new environmental foliage fibers, and discussed making good use of the natural sources and the trash from the agriculture has been an obvious characteristic of developing green fiber and eco-textile in this paper as while. This paper further analyzes the development and market potential of the new environmental foliage fiber.
Coexpression of the High Molecular Weight Glutenin Subunit 1Ax1 and Puroindoline Improves Dough Mixing Properties in Durum Wheat (Triticum turgidum L. ssp. durum)
Yin Li, Qiong Wang, Xiaoyan Li, Xin Xiao, Fusheng Sun, Cheng Wang, Wei Hu, Zhijuan Feng, Junli Chang, Mingjie Chen, Yuesheng Wang, Kexiu Li, Guangxiao Yang, Guangyuan He
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0050057
Abstract: Wheat end-use quality mainly derives from two interrelated characteristics: the compositions of gluten proteins and grain hardness. The composition of gluten proteins determines dough rheological properties and thus confers the unique viscoelastic property on dough. One group of gluten proteins, high molecular weight glutenin subunits (HMW-GS), plays an important role in dough functional properties. On the other hand, grain hardness, which influences the milling process of flour, is controlled by Puroindoline a (Pina) and Puroindoline b (Pinb) genes. However, little is known about the combined effects of HMW-GS and PINs on dough functional properties. In this study, we crossed a Pina-expressing transgenic line with a 1Ax1-expressing line of durum wheat and screened out lines coexpressing 1Ax1 and Pina or lines expressing either 1Ax1 or Pina. Dough mixing analysis of these lines demonstrated that expression of 1Ax1 improved both dough strength and over-mixing tolerance, while expression of PINA detrimentally affected the dough resistance to extension. In lines coexpressing 1Ax1 and Pina, faster hydration of flour during mixing was observed possibly due to the lower water absorption and damaged starch caused by PINA expression. In addition, expression of 1Ax1 appeared to compensate the detrimental effect of PINA on dough resistance to extension. Consequently, coexpression of 1Ax1 and PINA in durum wheat had combined effects on dough mixing behaviors with a better dough strength and resistance to extension than those from lines expressing either 1Ax1 or Pina. The results in our study suggest that simultaneous modulation of dough strength and grain hardness in durum wheat could significantly improve its breadmaking quality and may not even impair its pastamaking potential. Therefore, coexpression of 1Ax1 and PINA in durum wheat has useful implications for breeding durum wheat with dual functionality (for pasta and bread) and may improve the economic values of durum wheat.
Atmospheric Pressure Room Temperature Plasma Jets Facilitate Oxidative and Nitrative Stress and Lead to Endoplasmic Reticulum Stress Dependent Apoptosis in HepG2 Cells
Shasha Zhao, Zilan Xiong, Xiang Mao, Dandan Meng, Qian Lei, Yin Li, Pengyi Deng, Mingjie Chen, Min Tu, Xinpei Lu, Guangxiao Yang, Guangyuan He
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0073665
Abstract: Atmospheric pressure room temperature plasma jets (APRTP-Js) that can emit a mixture of different active species have recently found entry in various medical applications. Apoptosis is a key event in APRTP-Js-induced cellular toxicity, but the exact biological mechanisms underlying remain elusive. Here, we explored the role of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in APRTP-Js-induced apoptosis using in vitro model of HepG2 cells. We found that APRTP-Js facilitated the accumulation of ROS and RNS in cells, which resulted in the compromised cellular antioxidant defense system, as evidenced by the inactivation of cellular antioxidants including glutathione (GSH), superoxide dismutase (SOD) and catalase. Nitrotyrosine and protein carbonyl content analysis indicated that APRTP-Js treatment caused nitrative and oxidative injury of cells. Meanwhile, intracellular calcium homeostasis was disturbed along with the alteration in the expressions of GRP78, CHOP and pro-caspase12. These effects accumulated and eventually culminated into the cellular dysfunction and endoplasmic reticulum stress (ER stress)-mediated apoptosis. The apoptosis could be markedly attenuated by N-acetylcysteine (NAC, a free radical scavenger), which confirmed the involvement of oxidative and nitrative stress in the process leading to HepG2 cell apoptosis by APRTP-Js treatment.
The Interactive Effects of Transgenically Overexpressed 1Ax1 with Various HMW-GS Combinations on Dough Quality by Introgression of Exogenous Subunits into an Elite Chinese Wheat Variety
Xiang Mao, Yin Li, Shasha Zhao, Jian Zhang, Qian Lei, Dandan Meng, Fengyun Ma, Wei Hu, Mingjie Chen, Junli Chang, Yuesheng Wang, Guangxiao Yang, Guangyuan He
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0078451
Abstract: Seed storage proteins in wheat endosperm, particularly high-molecular-weight glutenin subunits (HMW-GS), are primary determinants of dough properties, and affect both end-use quality and grain utilization of wheat (Triticum aestivum L). In order to investigate the interactive effects between the transgenically overexpressed 1Ax1 subunit with different HMW-GS on dough quality traits, we developed a set of 8 introgression lines (ILs) overexpressing the transgenic HMW-glutenin subunit 1Ax1 by introgression of this transgene from transgenic line B102-1-2/1 into an elite Chinese wheat variety Chuanmai107 (C107), using conventional crossing and backcrossing breeding technique. The donor C107 strain lacks 1Ax1 but contains the HMW-GS pairs 1Dx2+1Dy12 and 1Bx7+1By9. The resultant ILs showed robust and stable expression of 1Ax1 even after five generations of self-pollination, and crossing/backcrossing three times. In addition, overexpression of 1Ax1 was compensated by the endogenous gluten proteins. All ILs exhibited superior agronomic performance when compared to the transgenic parent line, B102-1-2/1. Mixograph results demonstrated that overexpressed 1Ax1 significantly improved dough strength, resistance to extension and over-mixing tolerance, in the targeted wheat cultivar C107. Further, comparisons among the ILs showed the interactive effects of endogenous subunits on dough properties when 1Ax1 was overexpressed: subunit pair 17+18 contributed to increased over-mixing tolerance of the dough; expression of the Glu-D1 allele maintained an appropriate balance between x-type and y-type subunits and thereby improved dough quality. It is consistent with ILs C4 (HMW-GS are 1, 17+18, 2+12) had the highest gluten index and Zeleny sedimentation value. This study demonstrates that wheat quality could be improved by using transgenic wheat overexpressing HMW-GS and the feasibility of using such transgenic lines in wheat quality breeding programs.
TaCIPK29, a CBL-Interacting Protein Kinase Gene from Wheat, Confers Salt Stress Tolerance in Transgenic Tobacco
Xiaomin Deng, Wei Hu, Shuya Wei, Shiyi Zhou, Fan Zhang, Jiapeng Han, Lihong Chen, Yin Li, Jialu Feng, Bin Fang, Qingchen Luo, Shasha Li, Yunyi Liu, Guangxiao Yang, Guangyuan He
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0069881
Abstract: Calcineurin B-like protein-interacting protein kinases (CIPKs) have been found to be responsive to abiotic stress. However, their precise functions and the related molecular mechanisms in abiotic stress tolerance are not completely understood, especially in wheat. In the present study, TaCIPK29 was identified as a new member of CIPK gene family in wheat. TaCIPK29 transcript increased after NaCl, cold, methyl viologen (MV), abscisic acid (ABA) and ethylene treatments. Over-expression of TaCIPK29 in tobacco resulted in increased salt tolerance, which was demonstrated by higher germination rates, longer root lengths and better growth status of transgenic tobacco plants compared to controls when both were treated with salt stress. Physiological measurements indicated that transgenic tobacco seedlings retained high K+/Na+ ratios and Ca2+ content by up-regulating some transporter genes expression and also possessed lower H2O2 levels and reduced membrane injury by increasing the expression and activities of catalase (CAT) and peroxidase (POD) under salt stress. Moreover, transgenic lines conferred tolerance to oxidative stress by increasing the activity and expression of CAT. Finally, TaCIPK29 was located throughout cells and it preferentially interacted with TaCBL2, TaCBL3, NtCBL2, NtCBL3 and NtCAT1. Taken together, our results showed that TaCIPK29 functions as a positive factor under salt stress and is involved in regulating cations and reactive oxygen species (ROS) homeostasis.
Overexpression of Avenin-Like b Proteins in Bread Wheat (Triticum aestivum L.) Improves Dough Mixing Properties by Their Incorporation into Glutenin Polymers
Fengyun Ma, Miao Li, Tingting Li, Wei Liu, Yunyi Liu, Yin Li, Wei Hu, Qian Zheng, Yaqiong Wang, Kexiu Li, Junli Chang, Mingjie Chen, Guangxiao Yang, Yuesheng Wang, Guangyuan He
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0066758
Abstract: Avenin-like b proteins are a small family of wheat storage proteins, each containing 18 or 19 cysteine residues. The role of these proteins, with high numbers of cysteine residues, in determining the functional properties of wheat flour is unclear. In the present study, two transgenic lines of the bread wheat overexpressing avenin-like b gene were generated to investigate the effects of Avenin-like b proteins on dough mixing properties. Sodium dodecyl sulfate sedimentation (SDSS) test and Mixograph analysis of these lines demonstrated that overexpression of Avenin-like b proteins in both transgenic wheat lines significantly increased SDSS volume and improved dough elasticity, mixing tolerance and resistance to extension. These changes were associated with the increased proportion of polymeric proteins due to the incorporation of overexpressed Avenin-like b proteins into the glutenin polymers. The results of this study were critical to confirm the hypothesis that Avenin-like b proteins could be integrated into glutenin polymers by inter-chain disulphide bonds, which could help understand the mechanism behind strengthening wheat dough strength.
AP2/ERF Transcription Factor in Rice: Genome-Wide Canvas and Syntenic Relationships between Monocots and Eudicots
Muhammad Rashid, He Guangyuan, Yang Guangxiao, Javeed Hussain and Yan Xu
Evolutionary Bioinformatics , 2012, DOI: 10.4137/EBO.S9369
Abstract: The transcription factor family intimately regulates gene expression in response to hormones, biotic and abiotic factors, symbiotic interactions, cell differentiation, and stress signalling pathways in plants. In this study, 170 AP2/ERF family genes are identified by phylogenetic analysis of the rice genome (Oryza sativa l. japonica) and they are divided into a total of 11 groups, including four major groups (AP2, ERF, DREB, and RAV), 10 subgroups, and two soloists. Gene structure analysis revealed that, at position-6, the amino acid threonine (Thr-6) is conserved in the double domain AP2 proteins compared to the amino acid arginine (Arg-6), which is preserved in the single domain of ERF proteins. In addition, the histidine (His) amino acid is found in both domains of the double domain AP2 protein, which is missing in single domain ERF proteins. Motif analysis indicates that most of the conserved motifs, apart from the AP2/ERF domain, are exclusively distributed among the specific clades in the phylogenetic tree and regulate plausible functions. Expression analysis reveals a widespread distribution of the rice AP2/ERF family genes within plant tissues. In the vegetative organs, the transcripts of these genes are found most abundant in the roots followed by the leaf and stem; whereas, in reproductive tissues, the gene expression of this family is observed high in the embryo and lemma. From chromosomal localization, it appears that repetition and tandem-duplication may contribute to the evolution of new genes in the rice genome. In this study, interspecies comparisons between rice and wheat reveal 34 rice loci and unveil the extent of collinearity between the two genomes. It was subsequently ascertained that chromosome-9 has more orthologous loci for CRT/DRE genes whereas chromosome-2 exhibits orthologs for ERF subfamily members. Maximum conserved synteny is found in chromosome-3 for AP2 double domain subfamily genes. Macrosynteny between rice and Arabidopsis, a distant, related genome, uncovered 11 homologs/orthologs loci in both genomes. The distribution of AP2/ERF family gene paralogs in Arabidopsis was most frequent in chromosome-1 followed by chromosome-5. In Arabidopsis, ERF subfamily gene orthologs are found on chromosome-1, chromosome-3, and chromosome-5, whereas DRE subfamily genes are found on chromosome-2 and chromosome-5. Orthologs for RAV and AP2 with double domains in Arabidopsis are located on chromosome-1 and chromosome-3, respectively. In conclusion, the data generated in this survey will be useful for conducting genomic research to determine the precise role of the AP2/ERF gene during stress responses with the ultimate goal of improving crops.
AP2/ERF Transcription Factor in Rice: Genome-Wide Canvas and Syntenic Relationships between Monocots and Eudicots
Muhammad Rashid,Guangyuan He,Yang Guangxiao,Javeed Hussain
Evolutionary Bioinformatics , 2012,
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