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Search Results: 1 - 10 of 95073 matches for " YANG Jing-Liang "
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Application of Gene Bioaugmentation in Treatment of Recalcitrant Chemicals
基因强化在难降解污染物生物处理和修复中的应用

LIU Chun,HUANG Xia,YANG Jing-Liang,
刘 春
,黄 霞,杨景亮

微生物学报 , 2008,
Abstract: One of the new approaches for bioaugmentation treatment of recalcitrant chemicals is gene bioaugmentation, which is helpful to improve the bioaugmentation stability when genetically engineered microorganisms are used. The mechanism of gene bioaugmentation, viz. horizontal gene transfer within microbial community, is introduced. The influence factors of gene bioaugmentation: gene vector, cell touch conditions and environment conditions, are discussed. The applications in soil bioremediation and wastewater treatment of gene bioaugmentation are also summarized. The limitation of gene bioaugmentation is also mentioned.
Application of Gene Bioaugmentation in Treatment of Recalcitrant Chemicals
基因强化在难降解污染物生物处理和修复中的应用

LIU Chun,HUANG Xia,YANG Jing-Liang,
刘 春
,黄 霞,杨景亮

微生物学通报 , 2008,
Abstract: One of the new approaches for bioaugmentation treatment of recalcitrant chemicals is gene bioaugmentation, which is helpful to improve the bioaugmentation stability when genetically engineered microorganisms are used. The mechanism of gene bioaugmentation, viz. horizontal gene transfer within microbial community, is introduced. The influence factors of gene bioaugmentation: gene vector, cell touch conditions and environment conditions, are discussed. The applications in soil bioremediation and wastewater treatment of gene bioaugmentation are also summarized. The limitation of gene bioaugmentation is also mentioned.
Leakage and Survival of Genetically Engineered Microorganism in the Environment Applied for Wastewater Bioaugmentation Treatment
废水生物强化中基因工程菌的流失和环境生存状况研究

LIU Chun,HUANG Xia,YANG Jing-liang,
刘春
,黄霞,杨景亮

环境科学 , 2008,
Abstract: Abstract: Genetically engineered microorganism (GEM) leaking from bioreactors to natural environment when applied for wastewater bioaugmentation treatment, will lead to potential ecological risk. An atrazine-degrading GEM was used in a conventional activated sludge bioreactor (CAS) and a membrane bioreactor (MBR) to investigate leaking density of GEM in the effluent. Survival of GEM in the simulated natural environments after leakage was also explored. The results showed that the maximum leakage happened at the initial time of GEM inoculation. When inoculating density was 1010 CFU/mL, the maximum leaking density from CAS was close to inoculating density as well as the maximum leaking density from MBR was only 102 CFU/mL. Leaking density was the key factor influencing GEM survival in the simulated environments. When leaking density from CAS reached to 1010 CFU/mL, GEM with high density would survive in the simulated water and soil environments for a long time (more than 30 days), which would lead to high potential ecological risk. On the contrary, when leaking density from MBR was 102 CFU/mL, GEM would disappear quickly in the simulated environments, which meant low potential ecological risk. Environmental conditions also affected GEM survival. Increasing water content and organic compounds content of soil as well as creating environmental selective pressure (adding atrazine) were profitable for GEM survival.
Analysis of methanogenic community of anaerobic granular sludge based on mcrA gene
基于mcrA基因的厌氧颗粒污泥产甲烷菌群分析

Liu Chu,Li Liang,Ma Jun-Ke,Wu Gen,Yang Jing-Liang,
Liu C
,Li L,Ma JK,Wu G,Yang JL

环境科学 , 2011,
Abstract: The methanogenic community in anaerobic granular sludge from a full-scale UASB treating avernectin wastewater was analyzed based on mcrA gene, compared to 16S rRNA gene. The results indicated that the diversity indices of methanogenic community, including Shannon diversity index, Margalef richness index and Berger-Parker dominance index, were no difference between mcrA gene-based and 16S rRNA gene-based PCR products analysis by DGGE, although their DGGE band patterns were different, implying that the diversity analysis of methanogenic community based on mcrA genes was consistent with 16S rRNA gene. The phylogenetic analysis of dominant methanogenic populations based on these two target genes also showed resemble and Methanobacteriales and Methanosarcinales were determined to be the main orders of methanogenic populations in anaerobic granular sludge. On the other hand, the difference in phylogenetic analysis suggested simultaneously some group-specific of the two target genes. The hybridization of methanogenic community in FISH analysis based on two target genes was almost identical except a little different hybridization areas. The average relative abundance of methanogenic community was 24.25%±6.47% detected by FISH based on mcrA gene, lower than that based on 16S rRNA gene (33.42%±2.34%). Then it could be concluded that the analysis of methanogenic community based on mcrA gene and 16S rRNA gene exhibited high resemblance and mcrA gene could used to be target gene for methanogenic community, as an alternative of 16S rRNA gene.
Interception Characteristics of MBR for Genetically Engineered Microorganism (GEM) at the Beginning of Operation
MBR运行初期对基因工程菌的截留特性研究

LIU Chun,YANG Jing-Liang,LI Zai-Xing,GUO Jian-Bo,
刘 春
,杨景亮,李再兴,郭建博

微生物学报 , 2008,
Abstract: The leakage of genetically engineered microorganism (GEM) at the beginning of operation was the important content for ecological risk assessment when GEM was inoculated in a membrane bioreactor (MBR) for bioaugmentation. The effects of different operating conditions on leaking density and intercepting efficiency of GEM were investigated in a submerged microfiltration MBR at the beginning of operation. The interception characteristics were also discussed. The results showed that different operating conditions had different influences on intercepting efficiency: higher sludge concentration was profit for interception, while higher aeration intensity and flux had opposite effects on interception. When the inoculated density of GEM was 1.0×1010 CFU/mL, the leaking densities varied from 1.0×102 CFU/mL to 2.5×102 CFU/mL under different operating conditions at the beginning of operation and the maximum intercepting efficiency could be higher than 8 lg. The main factors determining intercepting efficiency at the beginning of operation were membrane module interception, sludge adsorption as well as suspended GEM transfer inhibition and their contribution shares under certain conditions were 82.3%, 14.9% and 2.8%, respectively. Gel layer formation during MBR stable operation was helpful to increase intercepting efficiency. The contribution shares for GEM interception of membrane module, sludge and gel layer were 75.3%, 10.7% and 14.0%, respectively, under certain conditions.
Interception Characteristics of MBR for Genetically Engineered Microorganism (GEM) at the Beginning of Operation
MBR运行初期对基因工程菌的截留特性研究

LIU Chun,YANG Jing-Liang,LI Zai-Xing,GUO Jian-Bo,
刘 春
,杨景亮,李再兴,郭建博

微生物学通报 , 2008,
Abstract: The leakage of genetically engineered microorganism (GEM) at the beginning of operation was the important content for ecological risk assessment when GEM was inoculated in a membrane bioreactor (MBR) for bioaugmentation. The effects of different operating conditions on leaking density and intercepting efficiency of GEM were investigated in a submerged microfiltration MBR at the beginning of operation. The interception characteristics were also discussed. The results showed that different operating conditions had different influences on intercepting efficiency: higher sludge concentration was profit for interception, while higher aeration intensity and flux had opposite effects on interception. When the inoculated density of GEM was 1.0×1010 CFU/mL, the leaking densities varied from 1.0×102 CFU/mL to 2.5×102 CFU/mL under different operating conditions at the beginning of operation and the maximum intercepting efficiency could be higher than 8 lg. The main factors determining intercepting efficiency at the beginning of operation were membrane module interception, sludge adsorption as well as suspended GEM transfer inhibition and their contribution shares under certain conditions were 82.3%, 14.9% and 2.8%, respectively. Gel layer formation during MBR stable operation was helpful to increase intercepting efficiency. The contribution shares for GEM interception of membrane module, sludge and gel layer were 75.3%, 10.7% and 14.0%, respectively, under certain conditions.
Bioaugmented Treatment of Atrazine by Genetically Engineered Microorganism in Different Bioreactors
不同生物反应器中基因工程菌生物强化处理阿特拉津研究

GUO Yuan-ming,LIU Chun,GUO Ya-nan,YANG Jing-liang,LI Liang,MA Jun-ke,
郭渊明
,刘春,郭亚楠,杨景亮,李亮,马俊科

环境科学 , 2011,
Abstract: Removal of atrazine was investigated when genetically engineered microorganism (GEM) was inoculated into membrane bioreactor (MBR) and hybrid bioreactor for bioaugmentation.The performances of atrazine removal in two bioreactors were explored.The variations of GEM density and atzA gene abundance in two bioreactors were also determined.The results indicated that removal activities of COD and ammonia nitrogen were inhibited a little by atrazine and recovered after bioaugmentation by inoculated GEM.The better removal performance of COD and ammonia nitrogen was obtained in MBR.The biological removal efficiency of atrazine was improved significantly when bioaugmented treatment by GEM was applied.The atrazine removal increased gradually and the average removal rates reached up to 38.94% in MBR and 29.36% in hybrid bioreactor in the later running period.After inoculated,GEM densities in two bioreactors decreased rapidly and then tended to be constant.The stable GEM densities in MBR,suspended sludge and adherent biofilm of hybrid bioreactor were 5×103 CFU/mL,1.1×103 CFU/mL and 0.4×103 CFU/mL,respectively.Fluorescence in situ hybridization (FISH) was used to detect atzA gene in two bioreactors and the result indicated that the average relative abundances of atzA gene decreased initially and increased subsequently.The largest average relative abundance of atzA gene was obtained in MBR.The average relative abundance of atzA gene in adherent biofilm is larger than that in suspended sludge in the hybrid bioreactor.The horizontal transfer of atzA gene was the possible important reason responsible for high gene abundance.
Variation of functional bacteria during start-up and operation of partial nitrification process
短程硝化启动运行中功能菌群变化研究

CHEN Xiao-Xuan,LIU Chun,YANG Jing-Liang,ZHANG Run,YANG Hui-Na,
陈晓轩
,刘春,杨景亮,张润,杨辉娜

微生物学通报 , 2012,
Abstract: Objective] Partial nitrification-anammox is considered as the shortest?process for biological nitrogen removal and partial nitrification is the important part of this process. Methods] The variation of functional bacteria during start-up and stable operation of partial nitrification process was investigated in a SBR bioreactor in this study. Results] The results indicated that ammonia-oxidizing bacteria (AOB) population was expanded significantly and nitrite-oxidizing bacteria (NOB) population was inhibited when DO concentration was controlled lower than 1 mg/L and ammonia loading of the influent increased gradually. As a result, start-up and stable operation of partial nitrification process was realized. When ammonia volumetric loading of the influent was 0.055 kg/(m3·d), the average ammonia removal volumetric loading and sludge loading were 0.043 kg/(m3·d) and 0.16?kg/(kg·d), respectively. In addition, the average nitrite accumulation rate was 83.4% at this time. AOB population density and relative abundance increased from 4.5×104 CFU/mL to 1.5×107CFU/mL and from 0.18% to 7.25%, respectively, during start-up and stable operation of partial nitrification process. At the same time, NOB population density and relative abundance decreased from 2.0×105 CFU/mL to 1.5×104 CFU/mL?and from 5.51% to 2.14%, respectively. Conclusion] The expansion of AOB population was responsible for realization of partial nitrification and ammonia removal. High ammonia concentration and loading?also caused the activity inhibition of partial nitrification.
A Method for Total DNA Extraction of Sludge Samples from Full-scale Wastewater Treatment Bioreactors
工业化废水处理反应器污泥总DNA提取方法

ZHANG Su,LIU Chun,YANG Jing-Liang,GUO Jian-Bo,LI Zai-Xing,
张 苏
,刘 春,杨景亮,郭建博,李再兴

微生物学通报 , 2008,
Abstract: According to the characteristics of sludge samples from full-scale wastewater treatment bioreactors, the essential total DNA extraction method for most environmental samples, lysozyme-SDS-phenol/ chloroform method, was modified to improve sample pretreatment, intensify cell lysis and enhance the efficiency of impurity removal. Obtain a general total DNA extraction method for industrial sludge samples. Such a method was applied for total DNA extraction of sludge samples from several running full-scale anaerobic or aerobic bioreactors in Shijiazhuang, China. The results indicated that the modified method was suitable for all the sludge samples in this study, showing the satisfying generality. The extracted total DNA of all sludge samples were pure, with about 1.8 of A260/ A280 ratio. The method was also efficient; with average total DNA yield of over 0.7 mg/g and maximum yield of 0.85 mg/g. Moreover, all the extracted total DNA samples could serve as templates directly to amplify 16S rDNA by PCR. The PCR products could be separated well by denaturing gradient gel electrophoresis (DGGE) and the DGGE band patterns were clear enough to be used for further analysis. All these facts indicated that the total DNA extraction method provided in this study could meet the requirements of sludge samples research, from full-scale wastewater treatment bioreactors, using molecular biology technologies.
Analysis of Methanogenic Community of Anaerobic Granular Sludge in a Full-scale UASB Treating Avermectin Wastewater
阿维菌素废水工业化UASB颗粒污泥产甲烷菌群分析

LIU Chun,MA Jun-ke,WU Gen,YANG Jing-liang,GUO Jian-bo,LI Zai-xing,
刘春
,马俊科,吴根,杨景亮,郭建博,李再兴

环境科学 , 2010,
Abstract: Methanogens is considered to be important functional microbial population in anaerobic granular sludge.Fluorescence in situ hybridization was used to analyze methanogens of anaerobic granular sludge in a full-scale UASB treating avermectin wastewater.The results indicated that the distribution forms of methanogens,Methanobacteriales and Methanosarcinales were identical on the surface and inner face of granular sludge samples in different formation stages(with different diameters),although the relative abund...
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