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Search Results: 1 - 10 of 99477 matches for " YANG Feng-jie "
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From Web directories to lightweight ontology: natural language processing and formalization

JU Qi,YANG Feng-jie,

计算机应用研究 , 2010,
Abstract: 为了解决以自然语言表示节点标签的分类树很难通过自动软件agents来进行自动推理的问题,通过词性标志、词义辨析、连接词辨析和受约束的自然语言定义及转换等步骤,将分类树中每一个节点对应的自然语言标签转换成了机器能够识别的逻辑表达式,从而使整个分类树转换成了一个轻量级本体,它适合应用在数据整合的语义匹配、文档分类和语义搜索等方面的自动推理,从而促进了本体知识的自动化推理,为以后文本自动检索奠定基础。
Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD
Da-Ming Huang,Tian-Zhen Zhang,Feng-Jie Cui,Wen-Jing Sun,Li-Ming Zhao,Meng-Yi Yang,Ya-Juan Wang
Journal of Biomedicine and Biotechnology , 2011, DOI: 10.1155/2011/917232
Abstract: A procedure for simultaneous identification and quantification of canrenone and its biotransformed product 11-α-hydroxy-canrenone by high-performance liquid chromatography with ultraviolet detector (HPLC-UVD) and mass spectrometry (LC-MS) methods was proposed. The optimal determination variables on the HPLC-UVD or LC-MS coupled with a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μm) were set as follows: detection wavelength of 280 nm, mobile phase of water and methanol gradient elution, temperature for the chromatographic column of 30°C, flow rate of mobile phase of 0.8 mL/min, sample injection volume of 5 μL, and elution time of 40 min. The MS conditions were set as follows: the flow rate of sheath gas, aux gas, and sweep gas were kept at 35 arb, 5 arb, and 0 arb, respectively. The temperature of capillary was held at 300°C, and capillary voltage was set at 30.00 V. Tube lens were performed at 100.00 V. The proposed method was validated by linearity (2 ≥ 0.9910), average recovery (94.93%, RSD1.21%), precision (RSD ≤ 1.31%), limit of detection, and limit of quantification (LOD 0.1~0.12 mg/L, LOQ 0.5~0.67 mg/L), which proved to be affordable for simultaneously determining canrenone and its bio-transformed product 11-α-hydroxy-canrenone.
Rapid differentiation and identification of five species of Salmonella by FT-IR spectroscopy

YANG Li-jun,LI Zhao-jie,SONG Xiao-hu,WANG Jing,LIU Yu-min,CUI Feng-jie,

微生物学通报 , 2013,
Abstract: Objective] To establish a standard fourier transform infrared (FT-IR) spectral library and a FT-IR method of differentiation and identification of five species of Salmonella, viz Salmonella typhimurium, Salmonella enteritidis, Salmonella choleraesuis, Salmonella arizonae and Salmonella potsdam. Methods] FT-IR fingerprint absorption spectra of five species of Salmonella were collected and analyzed by chemometric methods. Results] A standard FT-IR spectral library was created and could be used to compare with those of the target Salmonella and identify them; two cluster models of principal component analysis (PCA) and hierarchical cluster analysis (HCA) were established and could differentiate and identify well the five species of Salmonella. Conclusion] As a rapid, easy-to-use and accurate technique, FT-IR spectroscopy is an effective tool to differentiate and identify five species of Salmonella studied here.
Effect of Extra-cellular Polymeric Substances on Filtration of Modified Non-woven Fabric in Membrane Bio-reactor

ZHANG Chun-hua,YANG Feng-lin,WANG Wen-jun,AN Xiao-wen,ZHANG Feng-jie,

环境科学 , 2008,
Abstract: The effect of extra-cellular polymeric substances (EPS) on filtration of polyvinyl alcohol modified polypropylene non-woven in submerged membrane bioreactor (SMBR) was investigated by statistical method. The results show that soluble extra-cellular polymeric substances (EPSs) of activated sludge on the non-woven modules surface, components (protein/carbohydrate, P/C) of EPSs and relative hydrophobicity (RH) have a significant influence on filtration performance of module B, the Pearson's correlation coefficient (r(p)) related to membrane fouling resistance are 0.868, 0.840, 0.890, respectively. Modified module can effectively restrict the adsorption of EPSs, can reduce the ratio of P/C in EPSs and can decrease the accumulation of activated sludge. After hydrophilic modification of non-woven, the filtration performance is improved obviously and the un-fouling performance is increased.
Biogeochemistry Responses and Spectral Characteristics of Rhus Chinensis Mill under Heavy Metal Contamination Stress

LI Qing-ting,YANG Feng-jie,ZHANG Bing,ZHANG Xi,ZHOU Guang-zhu,

遥感学报 , 2008,
Abstract: 利用遥感生物地球化学的原理和方法,分析了德兴铜矿的重金属污染状况和植物盐肤木的生物地球化学效应,对盐肤木生物地球化学效应的波谱特征进行了系统的提取和分析.研究发现酸、铜、镉等是德兴铜矿地区主要的环境污染因子,野外调查及样品的化验分析表明盐肤木对铜元素呈现一定的富集作用和很强的位移效应,是适合铜矿复垦的植被.利用导数光谱、包络线去除、红边效应、植被指数等光谱信息处理方法对盐肤木的野外波谱分析表明,随着叶片中Cu等金属元素含量的增大,其产生的毒化效应的波谱特征越明显,盐肤木叶片光谱反射率明显升高,波形蓝移,红边陡坡斜率增大,叶绿素吸收深度变浅,吸收中心稍有蓝移,水的吸收深度变浅,吸收中心位置红移,绿度指数变化明显.对波谱特征及其与重金属含量的相关关系综合分析后认为,红边特征、植被指数NDVI、叶绿素吸收深度与叶片铜含量关系显著,可以作为植被铜污染遥感图像特征提取的参考.

ZHOU Guang-Zhu,WANG Cui-Zhen,YANG Feng-Jie,LI Yin-Ming,

红外与毫米波学报 , 2009,
Abstract: The objects' spectrum is often contaminated by noise when it is collected in the open air.According to the principle of the spectrum collection,the noise was considered as one kind of multiplicative compound noise.By theoretical derivation,the combination of logarithm transform and wavelet transform was introduced into noise reduction.Multiplicative noise simulation test was carried out.And the results show that the spatial correlation algorithm is best suited for spectral data denoising,modulus maxima algo...

LI Yan,GUO Heng-Chang,AN Ran,QI Feng-Jie,YANG Hong,JIANG Hong-Bing,GONG Qi-Huang,

红外与毫米波学报 , 2005,
Abstract: Near infrared femtosecond laser micro/nano-fabrication using in transparent condensed matters like glass and polymers was reported. Femtosecond laser induced refractive index change was used for fabrication of gratings and other elements inside silica glass. Drilling from rear surface in contact with water, microholes with large aspect ratio were machined in soda-lime glass; With the change of laser focus inside two-photon absorption polymer, straight and wavy lines are polymerized.
Evolutionary Patterns in the Sequence and Structure of Transfer RNA: Early Origins of Archaea and Viruses
Feng-Jie Sun,Gustavo Caetano-Anollés
PLOS Computational Biology , 2008, DOI: 10.1371/journal.pcbi.1000018
Abstract: Transfer RNAs (tRNAs) are ancient molecules that are central to translation. Since they probably carry evolutionary signatures that were left behind when the living world diversified, we reconstructed phylogenies directly from the sequence and structure of tRNA using well-established phylogenetic methods. The trees placed tRNAs with long variable arms charging Sec, Tyr, Ser, and Leu consistently at the base of the rooted phylogenies, but failed to reveal groupings that would indicate clear evolutionary links to organismal origin or molecular functions. In order to uncover evolutionary patterns in the trees, we forced tRNAs into monophyletic groups using constraint analyses to generate timelines of organismal diversification and test competing evolutionary hypotheses. Remarkably, organismal timelines showed Archaea was the most ancestral superkingdom, followed by viruses, then superkingdoms Eukarya and Bacteria, in that order, supporting conclusions from recent phylogenomic studies of protein architecture. Strikingly, constraint analyses showed that the origin of viruses was not only ancient, but was linked to Archaea. Our findings have important implications. They support the notion that the archaeal lineage was very ancient, resulted in the first organismal divide, and predated diversification of tRNA function and specificity. Results are also consistent with the concept that viruses contributed to the development of the DNA replication machinery during the early diversification of the living world.
Evolutionary Patterns in the Sequence and Structure of Transfer RNA: A Window into Early Translation and the Genetic Code
Feng-Jie Sun, Gustavo Caetano-Anollés
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0002799
Abstract: Transfer RNA (tRNA) molecules play vital roles during protein synthesis. Their acceptor arms are aminoacylated with specific amino acid residues while their anticodons delimit codon specificity. The history of these two functions has been generally linked in evolutionary studies of the genetic code. However, these functions could have been differentially recruited as evolutionary signatures were left embedded in tRNA molecules. Here we built phylogenies derived from the sequence and structure of tRNA, we forced taxa into monophyletic groups using constraint analyses, tested competing evolutionary hypotheses, and generated timelines of amino acid charging and codon discovery. Charging of Sec, Tyr, Ser and Leu appeared ancient, while specificities related to Asn, Met, and Arg were derived. The timelines also uncovered an early role of the second and then first codon bases, identified codons for Ala and Pro as the most ancient, and revealed important evolutionary take-overs related to the loss of the long variable arm in tRNA. The lack of correlation between ancestries of amino acid charging and encoding indicated that the separate discoveries of these functions reflected independent histories of recruitment. These histories were probably curbed by co-options and important take-overs during early diversification of the living world.
The ancient history of the structure of ribonuclease P and the early origins of Archaea
Feng-Jie Sun, Gustavo Caetano-Anollés
BMC Bioinformatics , 2010, DOI: 10.1186/1471-2105-11-153
Abstract: To study the evolution of this complex, we constructed rooted phylogenetic trees of RPR molecules and substructures and estimated RPP age using a cladistic method that embeds structure directly into phylogenetic analysis. The general approach was used previously to study the evolution of tRNA, SINE RNA and 5S rRNA, the origins of metabolism, and the evolution and complexity of the protein world, and revealed here remarkable evolutionary patterns. Trees of molecules uncovered the tripartite nature of life and the early origin of archaeal RPRs. Trees of substructures showed molecules originated in stem P12 and were accessorized with a catalytic P1-P4 core structure before the first substructure was lost in Archaea. This core currently interacts with RPPs and ancient segments of the tRNA molecule. Finally, a census of protein domain structure in hundreds of genomes established RPPs appeared after the rise of metabolic enzymes at the onset of the protein world.The study provides a detailed account of the history and early diversification of a fundamental ribonucleoprotein and offers further evidence in support of the existence of a tripartite organismal world that originated by the segregation of archaeal lineages from an ancient community of primordial organisms.With few exceptions [1], ribonuclease P (RNase P) is one of two universal ribozymes (the other is the ribosome) that are present in all living organisms. This ribonucleoprotein is generally composed of an RNA subunit, the RNase P RNA (RPR), and one or more protein subunits, the RNase P proteins (RPPs) [2]. RNase P functions as a phosphodiesterase carrying out the 5' endonucleolytic cleavage of transfer RNA (tRNA) precursor transcripts (pre-tRNA) to form mature functional tRNAs [3-5]. Regions of the RPR that contribute to the recognition of the substrate cleavage sites [the tRNA pseudouridine (TΨC) loop and CCA tail] are well studied. Remarkably, the catalytic function can be conducted by the RNA subunit indepen
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