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Search Results: 1 - 10 of 36977 matches for " YAN Guoquan "
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A case of giant primary osteoma cutis successfully treated with tissue expansion and surgical excision
Wu Minzhi,Wang Yan,Zhang Dawei,Jia Guoquan
Indian Journal of Dermatology, Venereology and Leprology , 2011,
Abstract:
Twisting products of Hopf algebras and quantum double
Guoquan Hu
Chinese Science Bulletin , 1999, DOI: 10.1007/BF02885478
Abstract: Let σ be a skew pairing on the pair (B,H) of Hopf algebras, and A a left (B, H) bicomodule algebra. A new algebraA σ , called the twisting product ofA, is obtained by alternating the multiplication ofA using σ and the coactions onA byB andH. σ induces a skew pairing $\hat \sigma $ on (B B ccp,H H cp), and the regular comodule structures ofB andH induce a left (B Bccp, H Hcp) bicomodule algebra structure onH H, and the associated twisting product (B H)σ- is a Hopf algebra, with the tensor coalgebra structure; moreover,A σ remains a left (B H)σ- comodule algebra. In particular, a description of the Drinfeld double is obtained from the twisting point of view. In addition, smash products appear as special cases. Dually, the construction of twisting coproducts is introduced by using copairings, and the Drinfeld quantum codouble and some smash coproducts are described.
Coxiella burnetii Induces Apoptosis during Early Stage Infection via a Caspase-Independent Pathway in Human Monocytic THP-1 Cells
Yan Zhang, Guoquan Zhang, Laura R. Hendrix, Vernon L. Tesh, James E. Samuel
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0030841
Abstract: The ability of Coxiella burnetii to modulate host cell death may be a critical factor in disease development. In this study, human monocytic THP-1 cells were used to examine the ability of C. burnetii Nine Mile phase II (NMII) to modulate apoptotic signaling. Typical apoptotic cell morphological changes and DNA fragmentation were detected in NMII infected cells at an early stage of infection. FACS analysis using Annexin-V-PI double staining showed the induction of a significant number of apoptotic cells at an early stage of NMII infection. Double staining of apoptotic cell DNA and intracellular C. burnetii indicates that NMII infected cells undergoing apoptosis. Interestingly, caspase-3 was not cleaved in NMII infected cells and the caspase-inhibitor Z-VAD-fmk did not prevent NMII induced apoptosis. Surprisingly, the caspase-3 downstream substrate PARP was cleaved in NMII infected cells. These results suggest that NMII induces apoptosis during an early stage of infection through a caspase-independent pathway in THP-1 cells. In addition, NMII-infected monocytes were unable to prevent exogenous staurosporine-induced apoptotic death. Western blot analysis indicated that NMII infection induced the translocation of AIF from mitochondria into the nucleus. Cytochrome c release and cytosol-to-mitochondrial translocation of the pore-forming protein Bax in NMII infected cells occurred at 24 h post infection. These data suggest that NMII infection induced caspase-independent apoptosis through a mechanism involving cytochrome c release, cytosol-to-mitochondrial translocation of Bax and nuclear translocation of AIF in THP-1 monocytes. Furthermore, NMII infection increased TNF-α production and neutralization of TNF-α in NMII infected cells partially blocked PARP cleavage, suggesting TNF-α may play a role in the upstream signaling involved in NMII induced apoptosis. Antibiotic inhibition of C. burnetii RNA synthesis blocked NMII infection-induced PARP activation. These results suggest that both intracellular C. burnetii replication and secreted TNF-α contribute to NMII infection-triggered apoptosis during an early stage of infection.
Unilateral pedicle screws asymmetric tethering: an innovative method to create idiopathic deformity
Yonggang Zhang, Yan Wang, Guoquan Zheng, Xuesong Zhang, Ruyi Zhang, Wei Zhang
Journal of Orthopaedic Surgery and Research , 2007, DOI: 10.1186/1749-799x-2-18
Abstract: Various methods are performed to create idiopathic deformity. Among these methods, posterior asmmetric tethering of the spine shows satisfying result, but some drawbacks related to the current posterior asymmetric tether were still evident.Unilateral pedicle screws asymmetric tethering was performed to 14 female goats (age: 5–8 week-old, weight: 6–8 kg) in concave side in combination with convex rib resection. Dorsoventral and lateral plain radiographs were taken of each thoracic spine in the frontal and sagittal planes right after the surgery and later every 4 weeks.All animals ambulated freely after surgery. For technical reasons, 2 goats were excluded (one animal died for anesthetic during the surgery, and one animal was lost for instrumental fail due to postoperative infection). Radiography showed that 11 goats exhibited scoliosis with convex toward to the right side, and as the curve increased with time, only 1 goat showed nonprogressive. The initial scoliosis generated in the progressors after the procedures measured 29.0° on average (range 23.0°–38.5°) and increased to 43.0° on average (range 36.0°–58.0°) over 8 to 10 weeks. The average progression of 14.0° was measured. The curvature immediately after tethering surgery (the initial Cobb angle) did have a highly significant correlation with the final curvature (p < 0.001). The progressive goats showed an idiopathic-like deformity not only by radiography, but in general appearance.Unilateral pedicle screws asymmetric tethering is a practical method to create experimental scoliosis, especially for those who would like to study the correction of this deformity.Till today, the etiology of the scoliosis is still uncertain. Many theories have been proposed to explain its occurrence, and many attempts have been made to establish a suitable experimental model of scoliosis. People have been interested in the animal models not only suitable to investigate the pathogenesy and the development, but also to study correctio
Proteomic analysis of prolactinoma cells by immuno-laser capture microdissection combined with online two-dimensional nano-scale liquid chromatography/mass spectrometry
Yingchao Liu, Jinsong Wu, Guoquan Yan, Ruiping Hou, Dongxiao Zhuang, Luping Chen, Qi Pang, Jianhong Zhu
Proteome Science , 2010, DOI: 10.1186/1477-5956-8-2
Abstract: Proteins from immuno-LCM captured prolactin cells were digested; resulting peptides were separated by two dimensional-nanoscale liquid chromatography (2D-nanoLC/MS) and characterized by tandem mass spectrometry. All MS/MS spectrums were analyzed by SEQUEST against the human International Protein Index database and a specific prolactinoma proteome consisting of 2243 proteins was identified. This collection of identified proteins by far represents the largest and the most comprehensive database of proteome for prolactinoma. Category analysis of the proteome revealed a widely unbiased access to various proteins with diverse functional characteristics.This manuscript described a more comprehensive proteomic profile of prolactinomas compared to other previous published reports. Thanks to the application of immuno-LCM combined with online two-dimensional nano-scale liquid chromatography here permitted identification of more proteins and, to our best knowledge, generated the largest prolactinoma proteome. This enlarged proteome would contribute significantly to further understanding of prolactinoma tumorigenesis which is crucial to the management of prolactinomas.Prolactinomas are the most common pituitary tumors, representing 25%-44% of all pituitary adenoma cases [1]. Although most are pathologically benign and grow slowly, prolactinomas show many symptoms in patients: amenorrhea, galactorrhea and dysgenesis in female patients and infertility and erectile dysfunction in male. Moreover, a number of prolactinomas belie their histology by perisellar invasion and postoperative recurrence. Comprehensive molecular dissection of prolactinoma pathogenesis is demanded for further understanding of this kind of tumors. Increasing evidences suggest that characterization at DNA or RNA level alone would not be sufficient to elucidate the mechanisms of this disease as lots of posttranslational modifications exist and pituitary adenoma proteomics would offer an efficient means for a com
Discovery and Confirmation of O-GlcNAcylated Proteins in Rat Liver Mitochondria by Combination of Mass Spectrometry and Immunological Methods
Weiqian Cao, Jing Cao, Jiangming Huang, Jun Yao, Guoquan Yan, Haoqi Xu, Pengyuan Yang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0076399
Abstract: O-linked β-N-acetylglucosamine (O-GlcNAc) is an important post-translational modification (PTM) consisting of a single N-acetylglucosamine moiety attached via an O-β-glycosidic linkage to serine and threonine residues. Glycosylation with O-GlcNAc occurs on myriad nuclear and cytosolic proteins from almost all functional classes. However, with respect to O-GlcNAcylated proteins special in mitochondria, little attention has been paid. In this study, we combined mass spectrometry and immunological methods to perform global exploration of O-GlcNAcylated proteins specific in mitochondria of rat liver. First, highly purified mitochondrial proteins were obviously shown to be O-GlcNAcylated by immunoblot profiling. Then, β-elimination followed by Michael Addition with Dithiothreitol (BEMAD) treatment and LC-MS/MS were performed to enrich and identify O-GlcNAcylated mitochondrial proteins, resulting in an unambiguous assignment of 14 O-GlcNAcylation sites, mapping to 11 O-GlcNAcylated proteins. Furthermore, the identified O-GlcNAcylated mitochondrial proteins were fully validated by both electron transfer dissociation tandem mass spectrometry (ETD/MS/MS) and western blot. Thus, for the first time, our study definitely not only identified but also validated that some mitochondrial proteins in rat liver are O-GlcNAcylated. Interestingly, all of these O-GlcNAcylated mitochondrial proteins are enzymes, the majority of which are involved in a wide variety of biological processes, such as urea cycle, tricarboxylic acid cycle and lipid metabolism, indicating a role for protein O-GlcNAcylation in mitochondrial function.
Porous Ti6Al4V Scaffold Directly Fabricated by Sintering: Preparation and In Vivo Experiment
Xuesong Zhang,Guoquan Zheng,Jiaqi Wang,Yonggang Zhang,Guoqiang Zhang,Zhongli Li,Yan Wang
Journal of Nanomaterials , 2013, DOI: 10.1155/2013/205076
Abstract: The interface between the implant and host bone plays a key role in maintaining primary and long-term stability of the implants. Surface modification of implant can enhance bone ingrowth and increase bone formation to create firm osseointegration between the implant and host bone and reduce the risk of implant losing. This paper mainly focuses on the fabricating of 3-dimensiona interconnected porous titanium by sintering of Ti6Al4V powders, which could be processed to the surface of the implant shaft and was integrated with bone morphogenetic proteins (BMPs). The structure and mechanical property of porous Ti6Al4V was observed and tested. Implant shaft with surface of porous titanium was implanted into the femoral medullary cavity of dog after combining with BMPs. The results showed that the structure and elastic modulus of 3D interconnected porous titanium was similar to cancellous bone; porous titanium combined with BMP was found to have large amount of fibrous tissue with fibroblastic cells; bone formation was significantly greater in 6 weeks postoperatively than in 3 weeks after operation. Porous titanium fabricated by powders sintering and combined with BMPs could induce tissue formation and increase bone formation to create firm osseointegration between the implant and host bone. 1. Introduction The hip joint is a spherical joint between the femoral head and the acetabulum in the pelvis. It is a diarthrosis or synovial joint wrapped in a capsule that contains the synovial fluid (SF). The hip joint can transmit high dynamic loads and accommodate a wide range of movements because of the presence of the SF and the ball-in-socket geometry. Though its remarkable characteristics, the hip joint can be affected, more often in aged people, by chronic pain and diseases such as osteoarthritis, rheumatoid arthritis, bone tumors, or traumas. In these cases, the best clinical solution is the total hip replacement, a surgical procedure that replaces the unhealthy hip joint with an implant, preserving the synovial capsule [1–3]. Metallic materials are widely used for joint replacement and orthopedic and dental implants. Metals are more suitable for loading-bearing applications when compared with ceramics or polymeric materials because of their excellent mechanical property. Among various metallic biomaterials, titanium and its alloys are the most attractive metallic biomaterials for orthopedic and dental implants due to their excellent mechanical properties, biocompatibility, processability, and good corrosion resistance in recent years. However, a major problem
Design and Movement Simulation to the Cam of the Testing Device for Capacitor Encapsulation Equipment  [PDF]
Hongwei Fang, Guoquan Zhang, Jiaqing Jian
Open Journal of Modelling and Simulation (OJMSi) , 2014, DOI: 10.4236/ojmsi.2014.24015
Abstract: In this paper, in order to design a cam mechanism be up to the mustard, a set of methods are put forward that using the Visual Basic programming language based on solidworks to draw cam contour line and then get its 3D models and generate the cam motion simulation by the solidworks motion. In the end, it’s proved that the cam designed though this method met the requirement.
Combination of matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry for the analysis of intact glycopeptides from horseradish peroxidase
基质辅助激光解吸电离质谱和电喷雾电离质谱在辣根过氧化物酶糖肽结构分析中的应用

CHEN Yaohan,YAN Guoquan,ZHOU Xinwen,YANG Pengyuan,
陈瑶函
,晏国全,周新文,杨芃原

色谱 , 2010,
Abstract: 糖链结构的质谱解析是今后糖蛋白分析中的重要研究内容,其中完整糖肽的分析,由于可以同时获得糖基化位点和对应糖链的结构信息,更具有重要意义和研究前景。本工作对质谱软电离技术在完整糖肽分析中的应用进行了研究,其中包括了基质辅助激光解吸电离(matrix-assisted laser desorption ionization, MALDI)和电喷雾电离(electrospray ionization, ESI)技术。通过平行使用两种串联质谱(tandem mass spectrometry, MS/MS)分析策略: MALDI-MS/MS和ESI-MS/MS对目标糖蛋白——辣根过氧化物酶进行分析,并讨论了其互补性。结果表明,MALDI和ESI技术各有优劣,结合串联质谱分析,可获得糖肽的糖链结构信息;两条路线互补使用,在揭示蛋白质糖基化修饰(位点和结构)的研究中十分必要。
A Polymorphism (rs2295080) in mTOR Promoter Region and Its Association with Gastric Cancer in a Chinese Population
Ming Xu, Guoquan Tao, Meiyun Kang, Yan Gao, Haixia Zhu, Weida Gong, Meilin Wang, Dongmei Wu, Zhengdong Zhang, Qinghong Zhao
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0060080
Abstract: Background As an imperative part of PI3K/Akt/mTOR pathway, mammalian target of rapamycin (mTOR) has been demonstrated to increase in gastric cancer cells and tumors. Our research explored the relationship between single nucleotide polymorphism (SNP) rs2295080 in mTOR promoter region and the risk of gastric cancer (GC). Methods Seven hundred and fifty-three (753) gastric adenocarcinoma patients and 854 matched healthy subjects were recruited in the cancer association study and 60 tissues were used to test the expression of mTOR. Unconditional logistic regression was selected to evaluate the association between the rs2295080 T>G polymorphism and GC risk. We then examined the functionality of this promoter genetic variant by luciferase assay and EMSA. Results Individuals with G allele had a 23% decreased risk of GC, comparing with those carrying T allele (adjusted OR = 0.77, 95% CI = 0.65–0.92). This protective effect of G allele stood out better in male group. Meanwhile, GC patients carrying TG/GG genotype also displayed a decreased mRNA level of mTOR (P = 0.004). In luciferase assay, T allele tended to enhance the transcriptional activity of mTOR with an approximate 0.5-fold over G allele. Furthermore, EMSA tests explained that different alleles of rs2295080 displayed different affinities to some transcriptional factor. Conclusion The mTOR promoter polymorphism rs2295080 was significantly associated with GC risk. This SNP, which effectively influenced the expression of mTOR, may be a new biomarker of early diagnosis of gastric cancer and a suitable indicator of utilizing mTOR inhibitor for treatment of GC.
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