oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 23 )

2018 ( 206 )

2017 ( 196 )

2016 ( 184 )

Custom range...

Search Results: 1 - 10 of 11394 matches for " Xuetao Cao "
All listed articles are free for downloading (OA Articles)
Page 1 /11394
Display every page Item
Immune activation of erythroleukemia cells induced by interleukin 12
Yong Zhao,Xuetao Cao
Science China Life Sciences , 1998, DOI: 10.1007/BF02895109
Abstract: To investigate the antitumor activity of IL-12, the induction of differentiation of IL-12 was observed using erythroleukemia cells (FBL-3) as model. After incubation with 200 U/mL IL-12 for 48 h, DNA synthesis of FBL-3 cells in S phase decreased significantly; the expression of CD14 which is the specific marker of monocyte increased, the rate of NBT+ cells was apparently higher than that of the untreated FBL-3 cells. After treating FBL-3 cells with IL-12 for 72 h, the expression of 33D1 and NLDC145 which are the specific markers of dendritic cells increased markedly, the surface molecules such as MHC-11, B7-1, B7-2, and VCAM-1 were up-regulated; morphological observation showed two kinds of cells: some cells had a ruffled surface and plentiful lysosome; the others had many dendritic projections on the surface, and contained numerous mitochondria. Functionally, the IL-12-treated FBL-3 cells could apparently stimulate the proliferation of allogeneic and autologous T lymphocytes, and improve the specific cytotoxic activity of CTL on FBL-3 cells. These results indicated that erythroleukemia cells were induced by IL12 to differentiate into the monocytes and dendritic cells, then exhibited the antigen-presenting function. The data outline a new mechanism for IL-12 to treat leukemia.
Regulation of Toll-like receptor signaling in the innate immunity
HuaZhang An,Cheng Qian,XueTao Cao
Science China Life Sciences , 2010, DOI: 10.1007/s11427-010-0011-x
Abstract: Toll-like receptors sense invading pathogens by recognizing a wide variety of conserved pathogen-associated molecular patterns (PAMPs). The members of TLR family selectively utilize adaptor proteins MyD88, TRIF, TIRAP and TRAM to activate overlapping but distinct signal transduction pathways which trigger production of different panels of mediators such as proinflammatory cytokines and type I interferon. These mediators not only control innate immunity but also direct subsequently developed adaptive immunity. TLR activation is strictly and finely regulated at multiple levels of the signal transduction pathways.
Immune activation of erythroleukemia cells induced by interleukin 12

ZHAO Yong,CAO Xuetao,

中国科学C辑(英文版) , 1998,
Abstract: To investigate the antitumor activity of IL 12, the induction of differentiation of IL 12 was observed using erythroleukemia cells (FBL 3) as model. After incubation with 200 U/mL IL 12 for 48 h, DNA synthesis of FBL 3 cells in S phase decreased significantly; the expression of CD14 which is the specific marker of monocyte increased, the rate of NBT + cells was apparently higher than that of the untreated FBL 3 cells. After treating FBL 3 cells with IL 12 for 72 h, the expression of 33D1 and NLDC145 which are the specific markers of dendritic cells increased markedly, the surface molecules such as MHC II,B7 1, B7 2, and VCAM 1 were up regulated; morphological observation showed two kinds of cells: some cells had a ruffled surface and plentiful lysosome; the others had many dendritic projections on the surface, and contained numerous mitochondria. Functionally, the IL 12 treated FBL 3 cells could apparently stimulate the proliferation of allogeneic and autologous T lymphocytes, and improve the specific cytotoxic activity of CTL on FBL 3 cells. These results indicated that erythroleukemia cells were induced by IL 12 to differentiate into the monocytes and dendritic cells, then exhibited the antigen presenting function. The data outline a new mechanism for IL 12 to treat leukemia.
The plasticity of adult stem cells and their application in myocardial regenerative medicine
Feng CAO,Guoliang JIA,Lili NIU,Yunfang WANG,Xuetao PEI,
Feng CAO
,Guoliang JIA,Lili NIU,Yunfang WANG,Xuetao PEI,Feng CAO,Guoliang JIA,Lili NIU

老年心脏病学杂志(英文版) , 2004,
Abstract: Current therapies for myocardial infarction and congestive heart failure are limited in efficacy or in applicability.The plasticity of adult stem cells and cellular transplantation offer a novel therapeutic approach to im- prove cardiac function.This review describes the latest progress in research,summarizes recent studies of adult stem cells and their application in myocardial regenerative medicine in China and abroad,and discusses the future direc- tions of cell transplantation as a new therapy to repair injured hearts.(J Geriatr Cardiol 2004;1(2):77-82.)
Inducible MicroRNA-223 Down-Regulation Promotes TLR-Triggered IL-6 and IL-1β Production in Macrophages by Targeting STAT3
Qingyun Chen, Hui Wang, Yang Liu, Yinjing Song, Lihua Lai, Quan Han, Xuetao Cao, Qingqing Wang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0042971
Abstract: MicroRNAs are small non-coding RNA molecules that regulate gene expression by either translational inhibition or mRNA degradation. MicroRNAs play pivotal roles in the regulation of both innate and adaptive immune responses, including TLR-triggered inflammatory response. Here we reported that the expression of microRNA-223 (miR-223) was significantly decreased in murine macrophages during activation by lipopolysaccharide (LPS) or poly (I:C) stimulation. The inducible miR-223 down-regulation resulted in the activation of signal transducer and activator of transcription 3 (STAT3), which is directly targeted by miR-223, thus promoting the production of pro-inflammatory cytokines IL-6 and IL-1β, but not TNF-α. Interestingly, IL-6 was found to be a main factor in inducing the decrease in miR-223 expression after LPS stimulation, which formed a positive feedback loop to regulate IL-6 and IL-1β. Herein, our findings provide a new explanation characterizing the molecular mechanism responsible for the regulation of IL-6 production after TLR-triggered macrophage activation.
Therapeutic effects on experimental metastatic tumor-bearing mice by vaccination with GM-CSF gene-modified and tumor antigen-pulsed macrophages
Yizhi Yu,Xuetao Cao,Hong Lei,Minghui Zhang,Weiping Zhang,Xuejun Zhu,Tianxing Ye,Jianli Wang
Science China Life Sciences , 1998, DOI: 10.1007/BF02882713
Abstract: Macrophages, with potent cytotoxic and antigen-presenting activities, can be used in cancer treatment. The biological characteristics and antitumor effect of GM-CSF gene-modified and tumor antigen-pulsed macrophages were investigated. The high levels of GM-CSF could be detected in the supernatants of macrophages after gene transfer. The cytotoxicity and the expression of MHC class II molecules of the gene-modified macrophages increased significantly and the antigen-presenting ability was enhanced. The gene-modified macrophages were then pulsed with tumor antigen and used to treat the experimental pulmonary metastastic mice. The number of pulmonary metastases was reduced significantly and the cytotoxicity of the CTL induced from the splenocytes of the tumor-bearing mice also increased. The results demonstrated that adenovirus-mediated GM-CSF gene transfer can activate macrophages to some extent and GM-CSF gene-modified, antigen-pulsed macrophages may be a new type of effective effector cells in the immunogene therapy of cancer.
Enhanced antitumor effects of tumor antigen-pulsed dendritic cells by their transfection with GM-CSF gene
Xuetao Cao,Weiping Zhang,Shihua Ma,Minghui Zhang,Jianli Wang,Tianxing Ye
Science China Life Sciences , 1997, DOI: 10.1007/BF03183594
Abstract: To investigate the biological characterization and antitumor activities of GM-CSF gene-transfected dendritic cells, the splenic dendritic cells were infected with GM-CSF recombinant replication-deficient adenovirusesin vitro. Their enhanced expression of B7 was demonstrated by FACS analysis, and more potent stimulatory activity was confirmed by allogeneic MLR. Immunization of dendritic cells pulsed with irradiated B16 melanoma cells induced significant CTL and enabled host to resist the challenge of wild-type B16 cells. When they were transfected with GM-CSF gene subsequently, the induced CTL activity was higher, and the produced protection against B16 cell challenge and therapeutic effect on the mice with preestablished pulmonary metastases more effective. These data suggest that the dendritic cells pulsed with tumor antigen then transfected with GM-CSF gene can be used as an effective vaccine in tumor immunotherapy.
Effects of anesthesia-induced modest hypothermia on cellular radiation sensitivity
Yingsong Xiang,Gusheng Tang,Xiongfei Xu,Rujun Yang,Jianming Cai,Minghui Zhang,Xuetao Cao
Science China Life Sciences , 2002, DOI: 10.1360/02yc9009
Abstract: To assess the mechanisms of modest hypothermia (MH) and its effects on cellular radiation response, a model of anesthesia-induced modest hypothermia (AIMH) in the adult mice and a model of pure MH in the newborn mice were established. The survival rate of lethally irradiated mice was increased to 72% through AIMH before irradiation. Both apoptosis and necrosis of human fetal bone marrow CD34+ hematopoietic stem cells cultured under MH were significantly decreased as detected by MTT and flow cytometry, with three-color labeled by PE-CD34 +/ FITC-AnnexinV /7AAD. The survival and proliferation of mouse bone marrow MNC treated with MH after irradiation were also increased. The MH exerted similar protective effects on the leukemia cell lines A20, HL60, K562 to the normal bone marrow cells, but it enhanced the radiation sensitivity of leukemia cell line FBL3 and mouse melanoma B16F10. No effects have been found on the radiation sensitivity of those cells treated with MH before irradiation. The results also showed that MH mediated the effects on radiation sensitivity, in addition to increasing the oxygen tension. These results show different effects of MH on different cells: (i) AIMH exerts a protective effect on the normal hematopoietic stem cells, some leukemia cell lines A20, HL60, K562, and some neoplasma 3LL, LOVO. And MH exhibits a synthetic effect with anesthetic. (ii) MH enhances the radiation sensitivity of another leukemia and neoplasma cell lines FBL3, B16F10 and CT26. Therefore, AIMH has a potential to enhance the effects of radiation-therapy and decrease side effects on some tumors.
In vivo distribution and gene expression of genetically modified hepatocytes after intrasplenic transplantation
Weiping Zhang,Xuetao Cao,Xin Huang,Jianli Wang,Qun Tao,Tianxing Ye
Science China Life Sciences , 1997, DOI: 10.1007/BF03183596
Abstract: To investigate the feasibility and efficacy of liver gene therapy mediated by intrasplenic transplantation of genetically modified hepatocytes, the normal mouse liver cell line BNL CL. 2 cells were introduced with Neo-resistant (NeoR) gene or interleukin-2 (IL-2) genein vitro, and transplanted intrasplenically into normal syngeneic mice (2 x 106 cell/mouse); subsequently, the expressions of the introduced genesin vim were detected. The RT-PCR results showed that NeoR mRNA expressions were detectable in livers 24 h after transplantation and lasted over 11 weeks. Moreover, The NeoR mRNA was detected to be expressed temporarily in spleens (24 h–1 week) and lungs (24–96 h) after transplantation. After intrasplenic transplantation of IL-2 gene-modified BNL CL.2 cells, the stable expressions of IL-2 mRNA in the livers of transplanted mice were detectable by RT-PCR (24 h–11 weeks), and certain levels of IL-2 (5–40 pg/mL) remained in the peripheral blood. When IL-2 gene-modified BNL CL. 2 cells were transplanted intrasplenically to treat the metastatic liver colon carcinoma-bearing mice, the survival time of the treated mice was significantly prolonged. The data indicate that intrasplenic transplantation of genetically modified hepatocytes could allow for oriental distribution in host livers and long-term survival of the transplanted liver cells, and effective expression of exogenous genesin vim, suggesting that this can be a candidate approach to liver-directed gene therapy.
Cloning and characterization of a novel deletion mutant of heterogeneous nuclear ribonucleoprotein M4 from human dendritic cells
Xin Huang,Zhongliang Zhao,Zhenglong Yuan,Minghui Zhang,Xuejun Zhu,Guoyou Chen,Xuetao Cao
Science China Life Sciences , 2000, DOI: 10.1007/BF02882286
Abstract: To identify differentially expressed genes from antigen-stimulated human dendritic cells (DC), subtractive cloning was adopted and more than ten novel genes differentially expressed were cloned. One is a deletion mutant of heterogeneous nuclear ribonucleoprotein (hnRNP) M4 in which the residues from 159 to 197 of hnRNP M4 have been absent. The deletion mutant was shown to be co-expressed with hnRNP M4 in cell lines. The mutant was expressed in antigen-stimulated DC but not in normal DC. Northern blot analysis revealed the presence of a major hnRNP M4 deletion mutant mRNA transcript of 2.4 kilobase with the highest levels in peripheral lymphocytes, lung, liver and spleen. It was also expressed in bone marrow-derived stromal cells (BMSC), BMSC treated with several cytokines but not in BMSC treated with TNF-α. The results revealed a new member of hnRNP family and suggested that hnRNP would participate in antigen process and presentation.
Page 1 /11394
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.