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Search Results: 1 - 10 of 127057 matches for " Xinglin Li "
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Quality Analysis on the Engineering Master of Biotechnology Specialty from Enterprise  [PDF]
Xinglin Li, Yang Han, Aijie Cao, Jun Zhao, Liming Zhang, Shuli Man, Wenyuan Gao
Creative Education (CE) , 2012, DOI: 10.4236/ce.2012.37B022
Abstract: In order to express the engineering master’s culture status of biotechnology from some enterprises, by questionnaire, 27 engineering masters from some enterprises and 22 academic masters from two universities, were investigated on the graduate students’ origin, their tutors’ title, the enterprise size and classify, their dissertation theme emphasis and classify, and the experiment condition for their dissertations. The results indicated that, the engineering masters were better than the academic masters on the practice condition and the practice chances, whereas, the academic masters markedly excelled the engineering masters on the tutors’ level, the test condition, and the innovation environment of science and technology.
OSW-1 Induced Apoptosis in Hepatocellular Carcinoma through Generation of ROS, Cytochrome C and Noxa Activation Independent of p53 with Non-Activation of Caspase-3  [PDF]
Xiaochen Liu, Jingchao Liang, Jichun Jin, Haiyang Li, Bosheng Mei, Xinglin Jin
Chinese Medicine (CM) , 2017, DOI: 10.4236/cm.2017.81001
Abstract: Aim: To study the antitumor mechanism of OSW-1 in hepatocellular carcinoma. Materials and Methods: The expression profiling microarray was carried out to extract RNA from SK-Hep-1 which suffered from OSW-1. ρ0-SK-Hep-1 was maintained SK-Hep-1 in MEM containing 100 μg/L ethidium bromide (EB), 1 mM sodium pyruvate and 50 μg/ml uridine for 40 days. Then confirmed COX-I and COX-II of mitochondrial DNA were knocked out. Cells suffered from OSW-1 or doxorubicin. Then cells were washed twice with cold PBS and incubated with DCFH-DA. Fluorescent signal was recorded by using Infinite 200 Pro multimode Plate readers. Results: OSW-1 elevates generation of ROS and Cytochrome C which are associated with the induction of apoptosis in SK-Hep-1 cells. We also demonstrate that OSW-1 does not depend on p53 to up-regulate the BH3-only protein Noxa. What is more noteworthy that the Caspase-9 and FADD are down-regulated in above process. Conclusion: OSW-1 induced special apoptosis is different from the mitochondrial death pathway and the death receptor pathway and final result is not Caspase family’s activating. This provides a novel theory that nonmalignant cells are significantly less sensitive to OSW-1 than cancer cell lines.
Selecting Wheat Seeds of Moderate Phytate Using Colorimetric Method
Xinglin Li,Dandan Wang,Jie Gao,Xinyan Li
Journal of Agricultural Science , 2011, DOI: 10.5539/jas.v3n2p267
Abstract: A simple and effective method of determining the phytate content of a single wheat seed was considered necessary for selecting the materials of moderate phytate during early generations breeding. 23 wheat genotypes (16 winter wheat cultivars and 7 spring wheat cultivars) were firstly used as determining samples of phytate content (PC) and inorganic phosphorus content (IPC) of the whole meal, secondarily they were planted in two locations for two times (2004 and 2005). The all single plants were harvested by a plot from those sites, then 6 individual plants were randomly selected to save their seeds for each plot, and the seeds of other plants were grinded into whole meal to assay their PC and IPC. Subsequently, every seed from per individual plant was cut into two semi-grain seeds: the semi-grain seed without embryo (SGSWOE) and the semi-grain seed with embryo (SGSWE); the SGSWOE was carried out to assay colorimetric scoring values by inorganic phosphorus content (CSVIPC), while according to the CSVIPC index, the satisfied SGSWEs were saved to plant at next generation or the dissatisfied SGSWEs were eliminated. The results showed that there was significantly negative correlation between PC and IPC of the wheat meal from either the first samples or the materials harvested at those sites, and for the latter there was significantly negative correlation between PC and CSVIPC. There was no significant difference of CSVIPC among the SGSWEOs and SGSWE of the spikes and individual plants from the same plot or the same genotype at any sites. In conclusion the CSVIPC of the SGSWOEs can indirectly express PC level of the wheat meal: the higher the former was, the lower the latter was. So the CSVIPC of SGSWOE can be applied to assaying the level of corresponding SGSWE according to their index, and screening the individual plant of moderation phytate. Moreover, this method is characterized by high efficiency during breeding processing.
Physicochemical properties of starches from two different yam (Dioscorea opposita Thunb.) residues
Wang, Yugao;Zhang, Liming;Li, Xinglin;Gao, Wenyuan;
Brazilian Archives of Biology and Technology , 2011, DOI: 10.1590/S1516-89132011000200004
Abstract: the starches obtained from two different yam residues, which were treated with alkali(starch-a) or enzyme (starch-e), were studied and compared with yam starch isolated using ordinary method (starch-o) for morphological, crystalline pattern, thermal, and pasting properties. the results revealed that the amylose content of three starches ranged from 19.47 to 22.17%. the granule surfaces of starch-a and starch-e were as smooth as that of starch-o. the crystalline pattern of the three starches was a c-type. the transition temperatures (to, tp and tc) varied from 70.11 to 73.64, 79.23 to 81.74, and 84.30 to 86.65 oc, respectively. the starch-e showed the highest δ hgel, followed by the starch-a, while it was lowest for the starch-o. according to the viscosity measurement, starch-o had the lowest pasting temperature, highest peak viscosity and breakdown viscosity, which were contrary to those of starch-e.
Disruption of the BMEI0066 gene attenuates the virulence of Brucella melitensis and decreases its stress tolerance
Xinglin Zhang, Jie Ren, Na Li, Wenjuan Liu, Qingmin Wu
International Journal of Biological Sciences , 2009,
Abstract: Brucella melitensis is a facultative intracellular pathogen. An operon composed of BMEI0066, which encodes a two-component response regulator CenR, and BMEI0067, which encodes a cAMP-dependent protein kinase regulatory subunit, has been predicted to exist in many bacterial species. However, little is known about the function of this operon. In order to characterize this operon and assess its role in virulence, we constructed a marked deletion mutant of BMEI0066. The mutant was less able to withstand hyperosmotic conditions than wild-type (16M), but showed no significant difference with 16M when challenged by H2O2. The mutant also showed increased sensitivity to elevated temperature (42°C) and a reduced survival ratio under acidic conditions compared with 16M. The mutant failed to replicate in cultured murine macrophages and was rapidly cleared from the spleens of experimentally infected BALB/c mice. These findings suggest that these operon products make an important contribution to pathogenesis in mice, probably by allowing B. melitensis to adapt to the harsh environment encountered within host macrophages.
Ag85B DNA vaccine suppresses airway inflammation in a murine model of asthma
Jian Wu, Jun Xu, Chuang Cai, Xinglin Gao, Li Li, Nanshan Zhong
Respiratory Research , 2009, DOI: 10.1186/1465-9921-10-51
Abstract: In this study, the Ag85B gene was cloned into pMG plasmids to yield the pMG-Ag85B plasmid. The expression of Ag85B gene in murine bronchial epithelia cells was detected by Western blotting and immunohistochemical staining after intranasal immunization with reconstructed pMG-Ag85B plasmids. The protective effect of pMG-Ag85B plasmids immunization in airway inflammation was evaluated by histological examination and bronchoalveolar lavage (BAL). IL-4 and IFN-γ levels in the BAL and supernatant from splenocyte culture were determined using ELISA kits.The Ag85B gene was successfully expressed in murine bronchial epithelia cells by intranasal immunization with reconstructed pMG-Ag85B plasmids. Using a murine model of asthma induced by ovalbumin (OVA), pMG-Ag85B immunization significantly inhibited cellular infiltration across the airway epithelium with a 37% decrease in the total number of cells (9.6 ± 2.6 × 105/ml vs. 15.2 ± 3.0 × 105/ml, p < 0.05) and a 74% decrease in the number of eosinophils (1.4 ± 0.2 × 105/ml vs. 5.4 ± 1.1 × 105/ml, p < 0.01) compared with the OVA-sensitized control group. There was no difference in the number of neutrophils in BAL fluid between the pMG-Ag85B group, the OVA-sensitized control group and the empty pMG group. IL-4 production was significantly decreased in the BAL fluid (32.0 ± 7.6 pg/ml vs. 130.8 ± 32.6 pg/ml, p < 0.01) and in the splenocyte supernatant (5.1 ± 1.6 pg/ml vs. 10.1 ± 2.3 pg/ml, p < 0.05) in the pMG-Ag85B group compared with the OVA-sensitized control group, while IFN-γ production was increased in the BAL fluid (137.9 ± 25.6 pg/ml vs. 68.4 ± 15.3 pg/ml, p < 0.05) and in the splenocyte supernatant (20.1 ± 5.4 pg/ml vs. 11.3 ± 3.2 pg/ml, p < 0.05).In a murine model of asthma induced by OVA, intranasal immunization with pMG-Ag85B significantly reduced allergic airway inflammation with less eosinophil infiltration. This protective effect was associated with decreased IL-4 and increased IFN-γ production in the BAL fluid and in
Synchronization Control for Reticle Stage and Wafer Stage Based on Iterative Learning Control
Zhipeng Wua,Xinglin Chen
Information Technology Journal , 2012,
Abstract: In order to resolve the problem that the reticle stage and the wafer stage must move synchronously during the scanning process, a synchronization controller is designed based on iterative learning control theory. The control system is a typical master-slave synchronization structure and the reticle stage is chosen as the slave system. An open-loop type synchronization learning controller is added to the feedback control system of the reticle stage and the convergence in the iteration domain is proved. Simulation results show that the synchronization learning method can reduce the synchronization error evidently.
Performance of Series Connected GaAs Photovoltaic Converters under Multimode Optical Fiber Illumination
Tiqiang Shan,Xinglin Qi
Advances in OptoElectronics , 2014, DOI: 10.1155/2014/824181
Abstract: In many military and industrial applications, GaAs photovoltaic (PV) converters are connected in series in order to generate the required voltage compatible with most common electronics. Multimode optical fibers are usually used to carry high-intensity laser and illuminate the series connected GaAs PV converters in real time. However, multimode optical fiber illumination has a speckled intensity pattern. The series connected PV array is extremely sensitive to nonuniform illumination; its performance is limited severely by the converter that is illuminated the least. This paper quantifies the effects of multimode optical fiber illumination on the performance of series connected GaAs PV converters, analyzes the loss mechanisms due to speckles, and discusses the maximum illumination efficiency. In order to describe the illumination dependent behavior detailedly, modeling of the series connected PV array is accomplished based on the equivalent circuit for PV cells. Finally, a series of experiments are carried out to demonstrate the theory analysis. 1. Introduction An increasing application of GaAs PV converters is the conversion of monochromatic light into electrical power [1–5]. A typical application of this technology is the optical transmission of energy or power-by-light. Electrical energy delivery in power-by-light systems represents a promising alternative for copper wires [6]. In such systems, the laser light is mostly transmitted through an optical fiber and a PV receiver converts the optical energy into electrical energy. These systems are used in places where the use of electrical energy is not recommendable, taking advantage of the properties of optical fiber such as electrical insulation, immunity from EMI, RF, and lightning. Examples of these applications are found in fields such as the oil industry, remote sensing, aerospace, explosions, high voltage power lines, and nuclear plants [7, 8]. In these applications, the required voltage of the electronic circuit is generally higher than that supplied by a single GaAs PV converter; this value is about 1?V. Therefore, GaAs converters are always connected in series to match the required voltage. One of the principal advantages to pursuing this technology is that electric power can be generated in a far smaller volume than required by a DC-DC converter. This option is more compact and has the possibility of integrating the GaAs PV converters with an electronic circuit. Fiber illumination is important because some of today’s optical power transfer applications do not have line of sight between the
TRAF6 Affects RAC1 Expression and Apoptosis in SK-Hep1 Cells  [PDF]
Shanlei Zhang, Changshi Qian, Xiaochen Liu, Shengjun Piao, Xinglin Jin
Chinese Medicine (CM) , 2018, DOI: 10.4236/cm.2018.94011
Abstract:

RAC1 is a small-molecule G protein that regulates multiple cell cycle, cytoskeletal reorganization, cell migration, and apoptosis. FADD-dependent TRAIL can promote tumor metastasis through RAC1 and PI3K, and down-regulating RAC1 expression can reduce FasL-induced apoptosis. In addition, RIP1 bound to GTP acts as an activating protein for RAC1 and is involved in cytoskeletal reorganization. TRAF6 promotes migration and metastasis by regulating the RAS pathway in tumors. Thus, it is necessary to understand the interaction between RAC1 and TRAF6 as well as FADD and RIP1. In this study, we cultured hepatoma SK-Hep1 cells in vitro, specifically blocked the necroptosis pathway with Nec-1, and silenced FADD, RIP1 and TRAF6 gene expression using RNAi technology. At the same time, the expression of RAC1 was evaluated separately using RT-PCR and Western blot. The hepatoma SK-Hep1 cells survival rate was highest when the concentration of Nec-1 was 60 μM and the concentration of Z-vad-fmk was 20 μM. And the apoptosis rate of the transfected RAC1 siRNA cells was 3.59% compared with transfected siRNA cells 10.01% which was significantly decreased (P < 0.01). RAC1 could promote the occurrence of apoptosis in SK-Hep1 cells. RAC1 expression was suppressed in both protein and gene level in SK-Hep1 cells when the TRAF6 gene was silenced, but there was no significant change in RAC1 gene and protein expression when FADD and RIP1 genes were silenced. TRAF6 affects RAC1 expression and apoptosis in SK-Hep1 cells, while the FADD and RIP1 genes do not affect the role of RAC1. The TRAF6 gene is an important target in liver cancer cells.

Effect of IRAK1 on Apoptosis and Necroptosis of Hepatoma Cell Line SK-Hep1  [PDF]
Zhijia Pan, Xiaolei Zhang, Changshi Qian, Xiaochen Liu, Xinglin Jin
Chinese Medicine (CM) , 2019, DOI: 10.4236/cm.2019.101003
Abstract: Interleukin I receptor associated kinase 1 (IRAK1) is a downstream signal molecule of activated MyD88 recruitment, which can activate Fas associated death domain protein (FADD) to induce apoptosis. IRAK1 can also activate tumor necrosis factor-related factor 6 (TRAF6) and induce the expression of a series of downstream specific genes. IRAK1 is an essential factor in the induction of mitochondrial division and necroptosis. In the current study, RNAi technique was used to silence IRAK1, and the apoptosis and necroptosis rate of SK-Hep1 cells were detected by flow cytometry. The apoptosis and the necroptosis pathway of hepatoma SK-Hep1 cells were blocked separately, and the expressions of FADD, RIP1 and TRAF6 genes were silenced separately. The results showed when the expression of IRAK1 was down-regulated, the apoptosis and necroptosis rate of SK-Hep1 cells were significantly increased. With silenced FADD, RIP1 and TRAF6, respectively, the expression of IRAK1 protein had no significant change. However, the expression of IRAK1 mRNA decreased significantly (p < 0.01) after the silencing of RIP1 and TRAF6 genes, while the IRAK1 mRNA did not change significantly after the silencing of FADD genes; when z-VAD-FMK was interfered, the expression of IRAK1 mRNA decreased significantly after the silencing of TRAF6 genes, while the IRAK1 mRNA did not change significantly after the silencing of FADD and RIP1genes. The study shows that RAK1 gene inhibits apoptosis and necroptosis in SK-Hep1 cells. TRAF6 gene affected the role of IRAK1 in apoptosis and necroptosis, RIP1 gene affected the role of IRAK1 in apoptosis, while FADD gene did not affect the role of IRAK1 in apoptosis and necroptosis.
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