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Search Results: 1 - 10 of 127051 matches for " XingYi Li "
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A New Cost-Sensitive Decision Tree with Missing Values
Xingyi Liu
Asian Journal of Information Technology , 2012,
Abstract: Cost-sensitive learning is popular during the process of classification. Most researches focus on two costs for building cost-sensitive decision trees, such as, misclassification costs, test costs. In this study, a novel splitting attributes criterion is proposed firstly. And a test strategy combining discount costs for decreasing the misclassification cost is presented with missing values in test set after the cost-sensitive decision tree are constructed with missing values in training sets. Finally, the experimental results show our method outperform the existed methods in terms of the decrease of misclassification cost.
Preparation of alginate coated chitosan microparticles for vaccine delivery
XingYi Li, XiangYe Kong, Shuai Shi, XiuLing Zheng, Gang Guo, YuQuan Wei, ZhiYong Qian
BMC Biotechnology , 2008, DOI: 10.1186/1472-6750-8-89
Abstract: The prepared alginate coated BSA loaded chitosan microparticles had loading efficiency (LE) of 60% and loading capacity (LC) of 6% with mean diameter of about 1 μm. When the weight ratio of alginate/chitosan microparticles was greater than 2, the stable system could be obtained. The rapid charge inversion of BSA loaded chitosan microparticles (from +27 mv to -27.8 mv) was observed during the coating procedure which indicated the presence of alginate layer on the chitosan microparticles surfaces. According to the results obtained by scanning electron microscopy (SEM), the core-shell structure of BSA loaded chitosan microparticles was observed. Meanwhile, in vitro release study indicated that the initial burst release of BSA from alginate coated chitosan microparticles was lower than that observed from uncoated chitosan microparticles (40% in 8 h vs. about 84% in 0.5 h). SDS-polyacrylamide gel electrophoresis (SDS-PAGE) assay showed that alginate coating onto chitosan microparticles could effectively protect the BSA from degradation or hydrolysis in acidic condition for at least 2 h. The structural integrity of alginate modified chitosan microparticles incubated in PBS for 24 h was investigated by FTIR.The prepared alginate coated chitosan microparticles, with mean diameter of about 1 μm, was suitable for oral mucosal vaccine. Moreover, alginate coating onto the surface of chitosan microparticles could modulate the release behavior of BSA from alginate coated chitosan microparticles and could effectively protect model protein (BSA) from degradation in acidic medium in vitro for at least 2 h. In all, the prepared alginate coated chitosan microparticles might be an effective vehicle for oral administration of antigens.Development of an oral antigens (protein, and etc) delivery system for mucosal vaccine is a meaningful challenge for pharmaceutical scientists. The instability and poor absorption of antigens in gastrointestinal tract is major obstacles in the development
Investigation of an Adenovirus-Induced Respiratory Disease Outbreak  [PDF]
Xingyi Geng, Ji Zhang, Guoliang Yang
Advances in Infectious Diseases (AID) , 2013, DOI: 10.4236/aid.2013.34039
Abstract:

Objective: An epidemiological investigation was carried out in school X in Jinan, Shandong Province, China, to identify the cause, epidemiology, and etiological characteristics of a febrile respiratory disease outbreak; and therefore to control the dissemination. Methods: Both field epidemiological investigations and laboratory examinations were carried out. Results: Forty cases were identified, in which 38 cases were students and two were teachers. Clinical manifestations included fever, coughing, headache, and sore throat. A total of 21 pharyngeal swab specimens were collected and 18 tested positive for adenovirus. The adenovirus hexon gene was sequenced in three of the 18 positive specimens and the results showed a 100% homology with the standard HAdV-55 HEXO. Conclusions: The outbreak originated from an adenovirus-infected student, who spread the pathogen to her classmates and teacher. The teacher then further disseminated the disease within the school which led to 40 febrile respiratory infections.

 

Chitosan-Alginate Sponge: Preparation and Application in Curcumin Delivery for Dermal Wound Healing in Rat
Mei Dai,XiuLing Zheng,Xu Xu,XiangYe Kong,XingYi Li,Gang Guo,Feng Luo,Xia Zhao,YuQuan Wei,Zhiyong Qian
Journal of Biomedicine and Biotechnology , 2009, DOI: 10.1155/2009/595126
Abstract: A biodegradable sponge, composed of chitosan (CS) and sodium alginate (SA), was successfully obtained in this work. The sponge was ethereal and pliable. The chemical structure and morphology of the sponges was characterized by FTIR and SEM. The swelling ability, in vitro drug release and degradation behaviors, and an in vivo animal test were employed to confirm the applicability of this sponge as a wound dressing material. As the chitosan content in the sponge decreased, the swelling ability decreased. All types of the sponges exhibited biodegradable properties. The release of curcumin from the sponges could be controlled by the crosslinking degree. Curcumin could be released from the sponges in an extended period for up to 20 days. An in vivo animal test using SD rat showed that sponge had better effect than cotton gauze, and adding curcumin into the sponge enhanced the therapeutic healing effect.
MPprimer: a program for reliable multiplex PCR primer design
Zhiyong Shen, Wubin Qu, Wen Wang, Yiming Lu, Yonghong Wu, Zhifeng Li, Xingyi Hang, Xiaolei Wang, Dongsheng Zhao, Chenggang Zhang
BMC Bioinformatics , 2010, DOI: 10.1186/1471-2105-11-143
Abstract: A program named MPprimer was developed to help users for reliable multiplex PCR primer design. It employs the widely used primer design program Primer3 and the primer specificity evaluation program MFEprimer to design and evaluate the candidate primers based on genomic or transcript DNA database, followed by careful examination to avoid primer dimerization. The graph-expanding algorithm derived from the greedy algorithm was used to determine the optimal primer set combinations (PSCs) for multiplex PCR assay. In addition, MPprimer provides a virtual electrophotogram to help users choose the best PSC. The experimental validation from 2× to 5× plex PCR demonstrates the reliability of MPprimer. As another example, MPprimer is able to design the multiplex PCR primers for DMD (dystrophin gene which caused Duchenne Muscular Dystrophy), which has 79 exons, for 20×, 20×, 20×, 14×, and 5× plex PCR reactions in five tubes to detect underlying exon deletions.MPprimer is a valuable tool for designing specific, non-dimerizing primer set combinations with constrained amplicons size for multiplex PCR assays.Multiplex polymerase chain reaction (PCR), defined as the simultaneous amplification of multiple regions of a DNA template or multiple DNA templates through the use of multiple primer sets (PS, comprising a forward primer and a reverse primer) in one tube, has been widely used in diagnostic applications of clinical [1,2] and environmental microbiology studies [3]. The key step in running a successful multiplex PCR reaction is to design an optimal primer set combination (PSC, a group of PSs, PSCs for primer set combinations). It is well known that for conventional PCR, the optimal PS has the following standards or properties: 1) primer size: 18-30 bp; 2) product size: 100-500 bp; 3) melting temperature (Tm) of both forward and reverse primers: 58-65°C, with a temperature difference of less than 3°C; 4) GC content of primers: 40-60%; 5) ΔG (Gibbs free energy) of the last five resi
Reliability analysis of the Ahringer Caenorhabditis elegans RNAi feeding library: a guide for genome-wide screens
Wubin Qu, Changhong Ren, Yuan Li, Jinping Shi, Jiye Zhang, Xiaolei Wang, Xingyi Hang, Yiming Lu, Dongsheng Zhao, Chenggang Zhang
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-170
Abstract: Here we performed a reliability analysis on the Ahringer C. elegans RNAi feeding library, which contains 16,256 bacterial strains, using a bioinformatics approach. Results demonstrated that most (98.3%) of the bacterial strains in the library are reliable. However, we also found that 2,851 (17.54%) bacterial strains need to be re-annotated even they are reliable. Most of these bacterial strains are the clones having the retired gene names. Besides, 28 strains are grouped into unreliable category and 226 strains are marginal because of probably expressing unrelated double-stranded RNAs (dsRNAs). The accuracy of the prediction was further confirmed by direct sequencing analysis of 496 bacterial strains. Finally, a freely accessible database named CelRNAi (http://biocompute.bmi.ac.cn/CelRNAi/ webcite) was developed as a valuable complement resource for the feeding RNAi library by providing the predicted information on all bacterial strains. Moreover, submission of the direct sequencing result or any other annotations for the bacterial strains to the database are allowed and will be integrated into the CelRNAi database to improve the accuracy of the library. In addition, we provide five candidate primer sets for each of the unreliable and marginal bacterial strains for users to construct an alternative vector for their own RNAi studies.Because of the potential unreliability of the Ahringer C. elegans RNAi feeding library, we strongly suggest the user examine the reliability information of the bacterial strains in the CelRNAi database before performing RNAi experiments, as well as the post-RNAi experiment analysis.Kamath and Ahringer constructed an important RNA interference (RNAi) feeding library of bacterial strains corresponding to roughly 86% of the estimated 19,000 predicted genes in C. elegans in 2003 [1-3]. This RNAi feeding library has contributed largely to genome-wide functional studies of the C. elegans genes, including embryonic development [4], aging [5,6],
A proteomic view of Caenorhabditis elegans caused by short-term hypoxic stress
Hualing Li, Changhong Ren, Jinping Shi, Xingyi Hang, Feilong Zhang, Yan Gao, Yonghong Wu, Langlai Xu, Changsheng Chen, Chenggang Zhang
Proteome Science , 2010, DOI: 10.1186/1477-5956-8-49
Abstract: Here, we utilized a quantitative proteomic approach to evaluate changes in the expression patterns of proteins during the early response to hypoxia in C. elegans. Two-dimensional difference gel electrophoresis (2D-DIGE) was used to compare the proteomic maps of wild type C. elegans strain N2 under a 4-h hypoxia treatment (0.2% oxygen) and under normoxia (control). A subsequent analysis by MALDI-TOF-TOF-MS revealed nineteen protein spots that were differentially expressed. Nine of the protein spots were significantly upregulated, and ten were downregulated upon hypoxic stress. Three of the upregulated proteins were involved in cytoskeletal function (LEV-11, MLC-1, ACT-4), while another three upregulated (ATP-2, ATP-5, VHA-8) were ATP synthases functionally related to energy metabolism. Four ribosomal proteins (RPL-7, RPL-8, RPL-21, RPS-8) were downregulated, indicating a decrease in the level of protein translation upon hypoxic stress. The overexpression of tropomyosin (LEV-11) was further validated by Western blot. In addition, the mutant strain of lev-11(x12) also showed a hypoxia-sensitive phenotype in subsequent analyses, confirming the proteomic findings.Taken together, our data suggest that altered protein expression, structural protein remodeling, and the reduction of translation might play important roles in the early response to oxygen deprivation in C. elegans, and this information will help broaden our knowledge on the mechanism of hypoxia response.Hypoxic stress can induce apoptosis but also trigger adaptive mechanisms for cell survival. Mammalian cells respond to hypoxia by changes in the expression of numerous genes and proteins to increase anaerobic energy production, protect cells from hypoxic stress, and increase local angiogenesis [1,2]. Recently, the nematode Caenorhabditis elegans (C. elegans) has been proven to be an valuable model organism for studying the molecular response to hypoxia [3,4]. Although C. elegans is sensitive to hypoxic stress, r
The Research on Current Situation and Countermeasure of University Science Park in Gulou District of Nanjing, China  [PDF]
Songqiang Wu, Xiao Xiao, Wang Lu, Xingyi Shen, Xianting Tao
Technology and Investment (TI) , 2015, DOI: 10.4236/ti.2015.62010
Abstract: So far, the development of State University Science Park has become important force to promote regional economy development. This paper theoretically makes judgment over the effects of regional economy development of State University Science Park. Thus, taking State University Science Park of Gulou District in Nanjing as an example, the paper respectively elaborated the influence of State University Science Park on regional economic brands, services and information from the aspects of brand building, technology trade, and scientific and technical activities undertaking. In order to further improve and popularize the practices of State University Science Park of Gulou District in Nanjing, the paper proposed the corresponding policy inspiration: constantly stuck to the law of economic development and innovated the service function and approaches; made use of science and technology entering the park to organize innovation union to promote the capacity of regional scientific and technical innovation and broke the bottleneck of State University Science Park development to provide the regional economy with sustainable service.
Transcription and splicing regulation in human umbilical vein endothelial cells under hypoxic stress conditions by exon array
Xingyi Hang, Peiyao Li, Zhifeng Li, Wubin Qu, Ying Yu, Hualing Li, Zhiyong Shen, Hao Zheng, Yan Gao, Yonghong Wu, Minghua Deng, Zhixian Sun, Chenggang Zhang
BMC Genomics , 2009, DOI: 10.1186/1471-2164-10-126
Abstract: Human umbilical vein endothelial cells (HUVECs) were treated with cobalt chloride (CoCl2) both to mimic hypoxia and to induce cell apoptosis and alternative splicing responses. Cell apoptosis rate analysis indicated that HUVECs exposed to 300 μM CoCl2 for 24 hrs were initially counterbalancing apoptosis with cell survival. We therefore used the Affymetrix exon array system to determine genome-wide transcript- and exon-level differential expression. Other than 1583 differentially expressed transcripts, 342 alternatively spliced exons were detected and classified by different splicing types. Sixteen alternatively spliced exons were validated by RT-PCR. Furthermore, direct evidence for the ongoing balance between HUVEC survival and apoptosis was provided by Gene Ontology (GO) and protein function, as well as protein domain and pathway enrichment analyses of the differentially expressed transcripts. Importantly, a novel molecular module, in which the heat shock protein (HSP) families play a significant role, was found to be activated under mimicked hypoxia conditions. In addition, 46% of the transcripts containing stress-modulated exons were differentially expressed, indicating the possibility of combinatorial regulation of transcription and splicing.The exon array system effectively profiles gene expression and splicing on the genome-wide scale. Based on this approach, our data suggest that transcription and splicing not only regulate gene expression, but also carry out combinational regulation of the balance between survival and apoptosis of HUVECs under mimicked hypoxia conditions. Since cell survival following the apoptotic challenge is pivotal in angiogenesis during the development of many vascular diseases, our results may advance the knowledge of multilevel gene regulation in endothelial cells under physiological and pathological conditions.The balance between endothelial cell (EC) survival and apoptosis is an important cellular process involved in preserving blo
FAST CALCULATION OF THERMAL ACTION OF ACTUAL BEAM DURING THE LASER HEAT TREATMENT
激光热处理中实际光束热作用的快速分析

LI Junchang,LI Xingyi,CHEN Qinghua,FAN Zebin,CHEN Jinbo,
李俊昌
,李行一,陈庆华,樊则宾,陈劲波

材料研究学报 , 1998,
Abstract: This paper describes concisely the fast calculation method and the experimental test of the temperature field of an arbitrarily given beam during the laser infusible heat treatment, and discusses theoreticaIIy necessity for detecting laser power density.
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