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Search Results: 1 - 10 of 44873 matches for " Xing-Quan Zhu "
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Teaching human parasitology in China
Guanghui Zhao, Shenyi He, Lin Chen, Na Shi, Yang Bai, Xing-Quan Zhu
Parasites & Vectors , 2012, DOI: 10.1186/1756-3305-5-77
The complete mitochondrial genomes for three Toxocara species of human and animal health significance
Ming-Wei Li, Rui-Qing Lin, Hui-Qun Song, Xiang-Yun Wu, Xing-Quan Zhu
BMC Genomics , 2008, DOI: 10.1186/1471-2164-9-224
Abstract: The sizes of the entire mt genome are 14,322 bp for T. canis, 14029 bp for T. cati and 14266 bp for T. malaysiensis, respectively. These circular genomes are amongst the largest reported to date for all secernentean nematodes. Their relatively large sizes relate mainly to an increased length in the AT-rich region. The mt genomes of the three Toxocara species all encode 12 proteins, two ribosomal RNAs and 22 transfer RNA genes, but lack the ATP synthetase subunit 8 gene, which is consistent with all other species of Nematode studied to date, with the exception of Trichinella spiralis. All genes are transcribed in the same direction and have a nucleotide composition high in A and T, but low in G and C. The contents of A+T of the complete genomes are 68.57% for T. canis, 69.95% for T. cati and 68.86% for T. malaysiensis, among which the A+T for T. canis is the lowest among all nematodes studied to date. The AT bias had a significant effect on both the codon usage pattern and amino acid composition of proteins. The mt genome structures for three Toxocara species, including genes and non-coding regions, are in the same order as for Ascaris suum and Anisakis simplex, but differ from Ancylostoma duodenale, Necator americanus and Caenorhabditis elegans only in the location of the AT-rich region, whereas there are substantial differences when compared with Onchocerca volvulus,Dirofiliria immitis and Strongyloides stercoralis. Phylogenetic analyses based on concatenated amino acid sequences of 12 protein-coding genes revealed that the newly described species T. malaysiensis was more closely related to T. cati than to T. canis, consistent with results of a previous study using sequences of nuclear internal transcribed spacers as genetic markers.The present study determined the complete mt genome sequences for three roundworms of human and animal health significance, which provides mtDNA evidence for the validity of T. malaysiensis and also provides a foundation for studying th
Toxoplasma gondii infection in humans in China
Peng Zhou, Zhaoguo Chen, Hai-Long Li, Haihong Zheng, Shenyi He, Rui-Qing Lin, Xing-Quan Zhu
Parasites & Vectors , 2011, DOI: 10.1186/1756-3305-4-165
Abstract: Toxoplasmosis, a cosmopolitan disease in humans and most mammals, is caused by the opportunistic protozoan Toxoplasma gondii mainly through peroral infections, bloodstream infections and congenital acquired infections. It has been estimated that one third of the world population has been infected [1,2]. In most adults it does not cause serious illness, however, blindness and mental retardation can be caused in congenitally infected children and severe diseases in those with compromised immunity. A recent study indicated that infection with T. gondii is associated with abdominal hernia [3].Toxoplasma gondii needs both definitive hosts and intermediate hosts to complete its sexual and the asexual replication phases in life cycle. The sexual phase only occurs in the intestine of the definitive hosts, felids. All the warm-blooded animals, the intermediate hosts, become infected mainly by consuming food or drink contaminated by oocysts evacuated from felids and tissue cysts from other intermediate hosts [4]. Acute infection happens in the initial few days, with the rapidly growing replication of the tachyzoites. Tachyzoites switch to bradyzoites as time goes by and form tissue cysts parasitizing in host cells. It would be lethal in Toxoplasma infected immune-compromised patients if bradyzoites revert to tachyzoites. In addition to felids, intermediate hosts carried with tachyzoites or tissue cysts are also responsible for the spread of T. gondii. Peroral infection, congenital and blood infection are three major ways for the transmission of this parasite [5].The first human case of toxoplasmosis in China was report in 1964 in Jiangxi Province [6]. Many human cases were reported in China since the first epidemic survey on toxoplasmosis was carried out in Guangxi Province in 1978 [7]. However, toxoplasmosis cases in China are hardly recognized by western clinicians, for very little information from China was published in English. The rising prevalence of T. gondii infection
Sensitive and Specific Target Sequences Selected from Retrotransposons of Schistosoma japonicum for the Diagnosis of Schistosomiasis
Jun-Jie Guo equal contributor,Hua-Jun Zheng equal contributor,Jing Xu,Xing-Quan Zhu,Sheng-Yue Wang ,Chao-Ming Xia
PLOS Neglected Tropical Diseases , 2012, DOI: 10.1371/journal.pntd.0001579
Abstract: Background Schistosomiasis japonica is a serious debilitating and sometimes fatal disease. Accurate diagnostic tests play a key role in patient management and control of the disease. However, currently available diagnostic methods are not ideal, and the detection of the parasite DNA in blood samples has turned out to be one of the most promising tools for the diagnosis of schistosomiasis. In our previous investigations, a 230-bp sequence from the highly repetitive retrotransposon SjR2 was identified and it showed high sensitivity and specificity for detecting Schistosoma japonicum DNA in the sera of rabbit model and patients. Recently, 29 retrotransposons were found in S. japonicum genome by our group. The present study highlighted the key factors for selecting a new perspective sensitive target DNA sequence for the diagnosis of schistosomiasis, which can serve as example for other parasitic pathogens. Methodology/Principal Findings In this study, we demonstrated that the key factors based on the bioinformatic analysis for selecting target sequence are the higher genome proportion, repetitive complete copies and partial copies, and active ESTs than the others in the chromosome genome. New primers based on 25 novel retrotransposons and SjR2 were designed and their sensitivity and specificity for detecting S. japonicum DNA were compared. The results showed that a new 303-bp sequence from non-long terminal repeat (LTR) retrotransposon (SjCHGCS19) had high sensitivity and specificity. The 303-bp target sequence was amplified from the sera of rabbit model at 3 d post-infection by nested-PCR and it became negative at 17 weeks post-treatment. Furthermore, the percentage sensitivity of the nested-PCR was 97.67% in 43 serum samples of S. japonicum-infected patients. Conclusions/Significance Our findings highlighted the key factors based on the bioinformatic analysis for selecting target sequence from S. japonicum genome, which provide basis for establishing powerful molecular diagnostic techniques that can be used for monitoring early infection and therapy efficacy to support schistosomiasis control programs.
Sequence Variation in ROP9 Gene among Toxoplasma gondii Strains from Different Hosts and Geographical Locations
Jia Chen,Su-Fang Fang,Zhong-Yuan Li,Dong-Hui Zhou,Guo-Hua Liu,Xing-Quan Zhu
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.4288.4291
Abstract: Toxoplasma gondii infects a wide range of animals and humans causing toxoplasmosis. T. gondii rhoptry protein 9 (TgROP9) is expressed only in tachyzoite stage that might be involved in the early stages of invasion and this soluble protein is constituted with putative B-cell epitope but also can trigger a T cell response and exclusively react with CD4 + T cell clone. In this study, researchers examined sequence variation in ROP9 gene among seven T. gondii strains from different hosts and geographical localities by PCR amplification, sequence analysis and phylogenetic reconstruction using Maximum Parsimony (MP). The results showed that the entire genome sequence of the TgROP9 gene was 4555 bp in length and no size variation was detected among all the strains. In addition, there were 26 (0-0.7%) variable nucleotide positions among all isolates with 9 variations of these being in the coding region and the other 11 were distributed among the 4 introns. Phylogenetic analyses revealed that the TgROP9 sequences were not a suitable genetic marker to differentiate T. gondii strains of different genotypes from different hosts and geographical locations. This study determined the TgROP9 genomic sequences of seven T. gondii strains and demonstrated the existence of low sequence variation in TgROP9 gene, suggesting that T. gondii ROP9 may represent a good vaccine candidate against toxoplamosis.
Research advancement of agroecosystem health and its management

WU Lan-Fang,OUYANG Zhu,CHENG Wei- Xin,ZHANG Xing-Quan,

中国生态农业学报 , 2003,
Abstract: Ecosystem health is the goal of ecosystem management. The concept, evaluation indicators and evaluation method of agroecosystem health are stated in this paper. Its evaluation indicators and criteria should combine the society-economic indicators and resource-environmental indicators according to its structure and function. To appraise the capacity of agricultural resource and to determine the direction of agricultural production are the purpose of the assessment.
Ubiquitous Mining with Interactive Data Mining Agents
Xin-Dong Wu,Xing-Quan Zhu,Qi-Jun Chen,Fei-Yue Wang,

计算机科学技术学报 , 2009,
Abstract: Due to the increasing availability and sophistication of data recording techniques, multiple information sources and distributed computing are becoming the important trends of modern information systems. Many applications such as security informatics and social computing require a ubiquitous data analysis platform so that decisions can be made rapidly under distributed and dynamic system environments. Although data mining has now been popularly used to achieve such goals, building a data mining system is, however, a nontrivial task, which may require a complete understanding on numerous data mining techniques as well as solid programming skills. Employing agent techniques for data analysis thus becomes increasingly important, especially for users not familiar with engineering and computational sciences, to implement an effective ubiquitous mining platform. Such data mining agents should, in practice, be intelligent, complete, and compact. In this paper, we present an interactive data mining agent — OIDM (online interactive data mining), which provides three categories (classification, association analysis, and clustering) of data mining tools, and interacts with the user to facilitate the mining process. The interactive mining is accomplished through interviewing the user about the data mining task to gain efficient and intelligent data mining control. OIDM can help users find appropriate mining algorithms, refine and compare the mining process, and finally achieve the best mining results. Such interactive data mining agent techniques provide alternative solutions to rapidly deploy data mining techniques to broader areas of data intelligence and knowledge informatics.
Mitochondrial Genome of the Eyeworm, Thelazia callipaeda (Nematoda: Spirurida), as the First Representative from the Family Thelaziidae
Guo-Hua Liu,Robin B. Gasser ,Domenico Otranto,Min-Jun Xu,Ji-Long Shen,Namitha Mohandas,Dong-Hui Zhou,Xing-Quan Zhu
PLOS Neglected Tropical Diseases , 2013, DOI: 10.1371/journal.pntd.0002029
Abstract: Human thelaziosis is an underestimated parasitic disease caused by Thelazia species (Spirurida: Thelaziidae). The oriental eyeworm, Thelazia callipaeda, infects a range of mammalian definitive hosts, including canids, felids and humans. Although this zoonotic parasite is of socio-economic significance in Asian countries, its genetics, epidemiology and biology are poorly understood. Mitochondrial (mt) DNA is known to provide useful genetic markers to underpin fundamental investigations, but no mt genome had been characterized for any members of the family Thelaziidae. In the present study, we sequenced and characterized the mt genome of T. callipaeda. This AT-rich (74.6%) mt genome (13,668 bp) is circular and contains 12 protein-coding genes, 22 transfer RNA genes and two ribosomal RNA genes, but lacks an atp8 gene. All protein-coding genes are transcribed in the same direction; the gene order is the same as those of Dirofilaria immitis and Setaria digitata (Onchocercidae), but distinct from Dracunculus medinensis (Dracunculidae) and Heliconema longissimum (Physalopteridae). Phylogenetic analyses of the concatenated amino acid sequence data for all 12 protein-coding genes by Bayesian inference (BI) showed that T. callipaeda (Thelaziidae) is related to the family Onchocercidae. This is the first mt genome of any member of the family Thelaziidae and should represent a new source of genetic markers for studying the epidemiology, ecology, population genetics and systematics of this parasite of humans and other mammals.
Mitochondrial and Nuclear Ribosomal DNA Evidence Supports the Existence of a New Trichuris Species in the Endangered Fran?ois’ Leaf-Monkey
Guo-Hua Liu, Robin B. Gasser, Peter Nejsum, Yan Wang, Qiang Chen, Hui-Qun Song, Xing-Quan Zhu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0066249
Abstract: The whipworm of humans, Trichuris trichiura, is responsible for a neglected tropical disease (NTD) of major importance in tropical and subtropical countries of the world. Whipworms also infect animal hosts, including pigs, dogs and non-human primates, cause clinical disease (trichuriasis) similar to that of humans. Although Trichuris species are usually considered to be host specific, it is not clear whether non-human primates are infected with T. trichiura or other species. In the present study, we sequenced the complete mitochondrial (mt) genome as well as the first and second internal transcribed spacers (ITS-1 and ITS-2) of Trichuris from the Fran?ois’ leaf-monkey (langur), and compared them with homologous sequences from human- and pig-derived Trichuris. In addition, sequence comparison of a conserved mt ribosomal gene among multiple individual whipworms revealed substantial nucleotide differences among these three host species but limited sequence variation within each of them. The molecular data indicate that the monkey-derived whipworm is a separate species from that of humans. Future work should focus on detailed population genetic and morphological studies (by electron microscopy) of whipworms from various non-humans primates and humans.
Identification and characterization of microRNAs in Clonorchis sinensis of human health significance
Min-Jun Xu, Quan Liu, Alasdair J Nisbet, Xian-Quan Cai, Chao Yan, Rui-Qing Lin, Zi-Guo Yuan, Hui-Qun Song, Xian-Hui He, Xing-Quan Zhu
BMC Genomics , 2010, DOI: 10.1186/1471-2164-11-521
Abstract: Here we report the use of this approach to identify and clone 6 new and 62,512 conserved C. sinensis miRNAs which belonged to 284 families. There was strong bias on families, family members and sequence nucleotides in C. sinensis. Uracil was the dominant nucleotide, particularly at positions 1, 14 and 22, which were located approximately at the beginning, middle and end of conserved miRNAs. There was no significant "seed region" at the first and ninth positions which were commonly found in human, animals and plants. Categorization of conserved miRNAs indicated that miRNAs of C. sinensis were still innovated and concentrated along three branches of the phylogenetic tree leading to bilaterians, insects and coelomates. There were two miRNA strategies in C. sinensis for its parasitic life: keeping a large category of miRNA families of different animals and keeping stringent conserved seed regions with high active innovation in other places of miRNAs mainly in the middle and the end, which were perfect for the parasite to perform its complex life style and for host changes.The present study represented the first large scale characterization of C. sinensis miRNAs, which have implications for understanding the complex biology of this zoonotic parasite, as well as miRNA studies of other related species such as Opisthorchis viverrini and Opisthorchis felineus of human and animal health significance.Fish-borne clonorchiasis, caused by the oriental liver fluke Clonorchis sinensis, is endemic in many Asian countries and over 35 million people globally are infected with C. sinensis, including an estimated 15 million in China [1]. The parasite has major socioeconomic impacts in other parts of Asia as well. In Korea, infection in humans is one of the most prevalent [2]. While in Vietnam, the prevalence reaches 79% in the Haiphong and Hanoi area [3]. This infection is also becoming increasingly common in non-endemic regions and in developed countries due to growing international ma
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