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Search Results: 1 - 10 of 120069 matches for " XinLe Wang "
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FFLO state with angle dependent Gap in Asymmetric Nuclear Matter
Xinle Shang,Pei Wang,Peng Yin,Wei Zuo
Physics , 2013, DOI: 10.1088/0954-3899/42/5/055105
Abstract: We consider the Fulde-Ferrell-Larkin-Ovchinnikov (FFLO) state with an angle dependent-gap (ADG) for the arbitrary angle \theta_0 between the direction of the Cooper pair momentum and the symmetry axis of the ADG in asymmetric nuclear matter. We find two kinds of locally stable states, i.e., the FFLO-ADG-orthogonal and FFLO-ADG-parallel states, which correspond to \theta_0=\pi/2 and \theta_0=0, respectively. Furthermore, the-FFLO-ADG-orthogonal state is located at small asymmetry, whereas the FFLO-ADG-parallel state is favored for large asymmetry. The critical isospin asymmetry \alpha_c, where the superfluid vanishes, is enhanced largely by considering the Cooper pair momentum with an ADG.
Green fluorescent protein (GFP) as a vital marker for studying the interaction of Phytophthora sojae and soybean
XiaoRen Chen,BaoPing Cheng,XinLe Wang,SuoMeng Dong,YongLin Wang,XiaoBo Zheng,YuanChao Wang
Chinese Science Bulletin , 2009, DOI: 10.1007/s11434-009-0417-7
Abstract: Transgenic Phytophthora sojae strains that produce green fluorescent protein (GFP) were obtained after stable DNA integration using the Hsp70 promoter and the Ham34 terminator of Bremia lactucae. The expression of GFP during different developmental stages of P. sojae was observed using fluorescent microscopy. Based on this reporter system, the histopathologic events caused by the pathogen in soybean leaves, hypocotyls and roots were monitored. Meanwhile, the difference in resistance between different soybean cultivars against P. sojae was analyzed microscopically in roots. The results indicate that GFP can be stably expressed in zoosporangia, zoospores, cysts, hyphae and oospores of P. sojae. Using the GFP marker, the infecting pathogens in leaves, hypocotyls and roots of host could be distinctly visualized. The germ tube length of cysts germinating on the roots of resistant cultivar Nannong 8848 was longer than that on the roots of susceptible cultivar Hefeng 35. These results show for the first time that this eukaryotic reporter can be used in P. sojae as a stable and vital marker, allowing the study of genetics of this hemibiotrophic pathogen.
Angle-dependent Gap state in Asymmetric Nuclear Matter
Xinle Shang,Wei Zuo
Physics , 2013, DOI: 10.1103/PhysRevC.88.025806
Abstract: We propose an axisymmetric angle-dependent gap (ADG) state with the broken rotational symmetry in isospin-asymmetric nuclear matter. In this state, the deformed Fermi spheres of neutrons and protons increase the pairing probabilities along the axis of symmetry breaking near the average Fermi surface. We find that the state possesses lower free energy and larger gap value than the angle-averaged gap state at large isospin asymmetries. These properties are mainly caused by the coupling of different m_{j} components of the pairing gap. Furthermore, we find the transition from the ADG state to the normal state is of second order and the ADG state vanishes at the critical isospin asymmetry m_{j} where the angle-averaged gap vanishes.
Determination of residues of cyromazine and its metabolite melamine in chickens by gas chromatography-mass spectrometry
气相色谱-质谱法测定鸡肉中残留的环丙胺嗪及其代谢物三聚氰胺

ZHU Xinle,LIU Qi,LI Dan,WANG Shuhuai,WANG Xia,
朱馨乐
,刘琪,李丹,王树槐,汪霞

色谱 , 2009,
Abstract: 建立了鸡肉中残留的环丙胺嗪及其代谢物三聚氰胺的气相色谱-质谱(GC-MS)检测方法。样品经酸化的乙腈-水溶液提取、二氯甲烷去脂、混合型的阳离子交换(MCX)固相萃取柱净化、N,O-双三甲基硅基-三氟乙酰胺(BSTFA)衍生化后进行GC-MS测定。环丙胺嗪采用外标法定量,三聚氰胺以三聚氰胺-15N3为内标定量。结果表明,环丙胺嗪与三聚氰胺的线性范围均为100~1000 μg/L,定量限为20 μg/kg。在20,40,80 μg/kg 3个添加浓度下目标化合物的平均回收率为75%~110%,批内、批间相对标准偏差分别小于10%和15%。该方法样品前处理简单,测定灵敏度高,定性准确,选择性好,可实现鸡肉中环丙胺嗪与三聚氰胺残留的同时测定。
Characterization of a FGF19 Variant with Altered Receptor Specificity Revealed a Central Role for FGFR1c in the Regulation of Glucose Metabolism
Hongfei Ge, Helene Baribault, Steven Vonderfecht, Bryan Lemon, Jennifer Weiszmann, Jonitha Gardner, Ki Jeong Lee, Jamila Gupte, Paramita Mookherjee, Minghan Wang, Jackie Sheng, Xinle Wu, Yang Li
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0033603
Abstract: Diabetes and associated metabolic conditions have reached pandemic proportions worldwide, and there is a clear unmet medical need for new therapies that are both effective and safe. FGF19 and FGF21 are distinctive members of the FGF family that function as endocrine hormones. Both have potent effects on normalizing glucose, lipid, and energy homeostasis, and therefore, represent attractive potential next generation therapies for combating the growing epidemics of type 2 diabetes and obesity. The mechanism responsible for these impressive metabolic effects remains unknown. While both FGF19 and FGF21 can activate FGFRs 1c, 2c, and 3c in the presence of co-receptor βKlotho in vitro, which receptor is responsible for the metabolic activities observed in vivo remains unknown. Here we have generated a variant of FGF19, FGF19-7, that has altered receptor specificity with a strong bias toward FGFR1c. We show that FGF19-7 is equally efficacious as wild type FGF19 in regulating glucose, lipid, and energy metabolism in both diet-induced obesity and leptin-deficient mouse models. These results are the first direct demonstration of the central role of the βKlotho/FGFR1c receptor complex in glucose and lipid regulation, and also strongly suggest that activation of this receptor complex alone might be sufficient to achieve all the metabolic functions of endocrine FGF molecules.
Pineal Photoreceptor Cells Are Required for Maintaining the Circadian Rhythms of Behavioral Visual Sensitivity in Zebrafish
Xinle Li, Jake Montgomery, Wesley Cheng, Jung Hyun Noh, David R. Hyde, Lei Li
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040508
Abstract: In non-mammalian vertebrates, the pineal gland functions as the central pacemaker that regulates the circadian rhythms of animal behavior and physiology. We generated a transgenic zebrafish line [Tg(Gnat2:gal4-VP16/UAS:nfsB-mCherry)] in which the E. coli nitroreductase is expressed in pineal photoreceptor cells. In developing embryos and young adults, the transgene is expressed in both retinal and pineal photoreceptor cells. During aging, the expression of the transgene in retinal photoreceptor cells gradually diminishes. By 8 months of age, the Gnat2 promoter-driven nitroreductase is no longer expressed in retinal photoreceptor cells, but its expression in pineal photoreceptor cells persists. This provides a tool for selective ablation of pineal photoreceptor cells, i.e., by treatments with metronidazole. In the absence of pineal photoreceptor cells, the behavioral visual sensitivity of the fish remains unchanged; however, the circadian rhythms of rod and cone sensitivity are diminished. Brief light exposures restore the circadian rhythms of behavioral visual sensitivity. Together, the data suggest that retinal photoreceptor cells respond to environmental cues and are capable of entraining the circadian rhythms of visual sensitivity; however, they are insufficient for maintaining the rhythms. Cellular signals from the pineal photoreceptor cells may be required for maintaining the circadian rhythms of visual sensitivity.
Test on Robustness of H∞ Autopilot by Sea Trials
H∞自动舵鲁棒性的海试验证

ZHANG Xianku,JIA Xinle,JIANG Dandong,
张显库
,贾欣乐,蒋丹东

控制理论与应用 , 2000,
Abstract: 给出了H∞鲁棒自动舵在阳澄湖号油轮上实际海试的结果,证明了该自动舵能够抑制海况干扰,具有良好的控制性能,对航速度化等产生的船舶模型摄动,具有良好的鲁棒性能。
三种不同磁共振扩散加权成像模型在鉴别乳腺良恶性病灶中的价值研究
Different models of diffusion-weighted magnetic resonance imaging in differential diagnosis of benign and malignant breast lesions

夏冰清,黎鑫乐,孙 琨,
XIA Bingqing
, LI Xinle, SUN Kun, et al

- , 2018, DOI: 10.19401/j.cnki.1007-3639.2018.05.007
Abstract: 背景与目的:近年来扩散加权成像(diffusion-weighted imaging,DWI)技术的开展及应用,大大提高了磁共振(magnetic resonance,MR)的特异度,其中体素内不相干运动(intravoxel incoherent motion,IVIM)模型和扩散峰度成像(diffusion kurtosis imaging,DKI)模型作为新兴技术,已在临床研究中取得一定的进展。该研究探讨DWI的单指数模型、IVIM模型和DKI模型在乳腺良恶性病灶中的鉴别诊断价值。方法:该研究为前瞻性研究,纳入标准:超声或X线BI-RADS 4类及以上患者。排除标准:① 乳腺MR检查前已进行穿刺检查、新辅助化疗或手术的患者;② 图像运动伪影较重。所有患者术前均行双侧乳腺MR检查,扫描序列包括快速反转恢复(turbo inversion recovery magnitude,TIRM)、多b值DWI(RS-EPI)和T1W动态增强扫描。选取病灶实性成分最大层面且避开明显坏死、囊变液化区绘制感兴趣区(region of interest,ROI),分别测量单指数模型参数表观弥散系数(apparent diffusion coefficient,DADC)值、IVIM模型参数[真实扩散系数(tissue diffusivity coefficient,DDT)、灌注相关扩散系数(perfusion-related diffusivity coefficient,D*)、灌注分数(perfusion fraction,f)]和DKI模型参数[峰度系数(kurtosis coefficient,K)、扩散系数(diffusivity coefficient,DDK)]。采用独立样本t检验分别比较乳腺良恶性病灶组织上述参数的差异。采用受试者工作特征(receiver operating characteristic,ROC)曲线评价3种模型参数的诊断效能。采用Z检验比较各参数曲线下面积(area under curve,AUC)的差异。结果:依据上述标准共纳入80例患者(83个病灶),其中良性病灶38个,恶性病灶45个。3种不同扩散模型中DADC值、DDT值、K值及DDK值在鉴别乳腺良恶性病灶中差异均有统计学意义(P均<0.05),其最佳阈值分别为DADC值1.08×10-3 mm2/s、DDT值1.06×10-3 mm2/s、K值0.756及DDK值1.36×10-3 mm2/s。而D*值和f值在良恶性病灶之间存在较大重叠,差异无统计学意义(P>0.05)。ROC曲线显示,K值和DDT值在鉴别乳腺良恶性病灶的AUC值最高,分别为0.956和0.947,K值的灵敏度和特异度为91.1%和89.5%,DDT值的灵敏度和特异度为93.3%和84.2%;DADC值和DDK值其次,AUC分别为0.933和0.923,DADC值的灵敏度和特异度为88.9%和84.2%,DDK值的灵敏度和特异度为91.1%和84.2%。最后,DADC值、DDT值、K值及DDK值在鉴别乳腺良恶性病灶中的ROC曲线的AUC差异均无统计学意义(P均>0.05)。结论:三种不同扩散加权成像模型在鉴别乳腺良恶性病灶中均有较好的诊断价值,其中IVIM和DKI的诊断效能较单指数模型略高,但 三者间差异无统计学意义。单指数模型扫描时间短,后处理简单,在临床应用价值很高。
我国禾谷缢管蚜微卫星位点扩增稳定性及遗传多样性
Amplifying stability and genetic diversity of microsatellite loci in different Rhopalosiphum padi (Linnaeus) populations in China

段辛乐,乔宪凤,彭雄,左亚运,刘高鸣,陈茂华,Duan Xinle,Qiao Xianfeng,Peng Xiong,Zuo Yayun,Liu Gaoming,Chen Maohua
- , 2015, DOI: 10.13802/j.cnki.zwbhxb.2015.03.002
Abstract: 为筛选用于我国禾谷缢管蚜种群遗传学研究的微卫星位点,从8个省(市)共9个地区采集282头试虫,采用微卫星PCR产物荧光标记与自动扫描分型方法,研究了8个欧洲禾谷缢管蚜微卫星位点在试虫个体中的扩增稳定性和遗传多样性.结果显示:位点R1.35在9个种群中均不能扩增;位点R5.29b只在7个种群的少数样本中能扩增;位点R2.73、R3.171、R5.10、R5.138、R5.50和R6.3在各种群中均能稳定扩增,这6个位点的效等位基因频率为0.0044~0.2663,平均等位基因数为2.9~9.3个,观测杂合度为0.047~0.912,其中位点R6.3具有较低的观测杂合度(0.047)和等位基因数(2.9),不适合用于种群遗传多样性研究,而位点R2.73、R3.171、R5.10、R5.138和R5.50均具有较高的杂合度和等位基因数,可用于我国禾谷缢管蚜的种群遗传学研究.
In order to screen polymorphic microsatellite loci which can be used for the population genetics research of Rhopalosiphum padi populations in China, the amplifying stability and genetic diversity of eight previously reported microsatellite loci were analyzed in 282 individuals sampled in nine regions of eight provinces in China. The PCR fragments of the microsatellite loci were labeled fluorescently and scanned automatically. The results showed that the locus R1.35 could not be amplified in any samples of the nine populations, and the locus R5.29b could only be amplified in a few samples of seven populations. Loci R2.73, R3.171, R5.10, R5.138, R5.50 and R6.3 could be amplified stably in samples of all populations. The null allele frequency (Na) of the six stably amplified loci was between 0.0044 and 0.2663; the mean number of allele (NA) ranged from 2.9 to 9.3, and the observed heterozygosity (Ho) was from 0.047 to 0.912. Locus R6.3 showed lower observed heterozygosity (0.047) and mean number of allele (2.9) than other loci, indicating that it could not be used for further population genetics analysis in China. Loci R2.73, R3.171, R5.10, R5.138 and R5.50 showed high observed heterozygosity and mean number of allele, suggesting that they are usable for the genetic diversity and genetic structure research of R. padi populations in China.
FGF21 Can Be Mimicked In Vitro and In Vivo by a Novel Anti-FGFR1c/β-Klotho Bispecific Protein
Richard Smith, Amy Duguay, Alice Bakker, Peng Li, Jennifer Weiszmann, Melissa R. Thomas, Benjamin M. Alba, Xinle Wu, Jamila Gupte, Li Yang, Jennitte Stevens, Agnes Hamburger, Stephen Smith, Jiyun Chen, Renee Komorowski, Kevin W. Moore, Murielle M. Véniant, Yang Li
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0061432
Abstract: The endocrine hormone FGF21 has attracted considerable interest as a potential therapeutic for treating diabetes and obesity. As an alternative to the native cytokine, we generated bispecific Avimer polypeptides that bind with high affinity and specificity to one of the receptor and coreceptor pairs used by FGF21, FGFR1c and β-Klotho. These Avimers exhibit FGF21-like activity in in vitro assays with potency greater than FGF21. In a study conducted in obese male cynomolgus monkeys, animals treated with an FGFR1c/β-Klotho bispecific Avimer showed improved metabolic parameters and reduced body weight comparable to the effects seen with FGF21. These results not only demonstrate the essential roles of FGFR1c and β-Klotho in mediating the metabolic effects of FGF21, they also describe a first bispecific activator of this unique receptor complex and provide validation for a novel therapeutic approach to target this potentially important pathway for treating diabetes and obesity.
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