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Search Results: 1 - 10 of 14478 matches for " Ximenes Filho "
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Intercoronary connection with bidirectional blood flow and concentric left ventricular hypertrophy
Meireles, George César Ximenes;Abreu Filho, Luciano Mauricio;
Sao Paulo Medical Journal , 1994, DOI: 10.1590/S1516-31801994000400008
Abstract: cardiac catheterization in a 55-year-old man, with a 6-month history of atypical chest pain and q-waves in d1, dili and avf, showed concentric left ventricular (lv) hypertrophy and a large intercoronary connection between right coronary artery (rca) and circumflex artery (cx), with bidirectional blood flow. although the rca and cx were normal, selective injection of cx filled rca retrogradely and in the same way selective injection of rca filled cx. possible mechanisms and literature are reviewed.
Production and characterization of an enzyme complex from a new strain of Clostridium thermocellum with emphasis on its xylanase activity
Vieira, Werner Bessa;Moreira, Leonora Rios de Souza;Monteiro Neto, Amadeu;Ferreira Filho, Edivaldo Ximenes;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000200009
Abstract: a new bacterial strain (iso ii) was isolated from manure cow and identified as phylogenetically close to the thermophilic cellulolytic bacterium clostridium thermocellum. the new strain produced extracellular xylanase, pectinase, mannanase and cellulase activities when grown in liquid culture medium containing banana stem as carbon source. the enzyme production profile after growth on banana stem showed that xylanase and cellulase activities were detected in different incubation periods. an enzyme complex containing xylanase, cellulase and mannanase activities was isolated from culture supernatant samples of strainiso ii. the complex was partially purified by ultrafiltration and gel filtration chromatography on sephacryl s-300. zymogram analysis after sds-page presented at least 05 subunits with xylanase activity. the enzyme showed single protein and xylanase activity bands after electrophoresis under non-denaturing conditions. the hydrolysis of xylan was optimal at temperature range of 55-75oc and ph 6.0. xylanase activity was quite stable at 65oc, retaining 80% of its original activity after 12 h incubation. the apparent km values, using insoluble and soluble arabinoxylans as substrates, were 1.54 and 11.53 mg/ml, respectively. xylanase was activated by dithiothreitol, l-tryptophan and l-cysteine and strongly inhibited by n-bromosuccinimide and cocl2. the characterization of mannanase showed km and temperature optimum of 0.846 mg/ml and 65oc, respectively and ph 8.0. by contrast to xylanase, it was less stable at 65oc with half-life of 2.5 h and inhibited by dithiothreitol and ca2+.
Purification and characterization of a new Xylanase from Humicola grisea var. Thermoidea
Lucena-Neto, Severino de Albuquerque;Ferreira-Filho, Edivaldo Ximenes;
Brazilian Journal of Microbiology , 2004, DOI: 10.1590/S1517-83822004000100014
Abstract: the thermophilic fungus humicola grisea var. thermoidea secretes extracellular xylanase when grown on solid and in liquid media containing wheat bran and banana plant residue as substrates, respectively. at 55oc, xylanase from the culture filtrate of h. grisea var. thermoidea grown on banana stalk retained 50% of its activity after 28 h of incubation. a xylanase (x2) was isolated from solid state cultures with wheat bran as the carbon source. it was purified to apparent homogeneity by ultrafiltration followed by ion-exchange and hydrophobic interaction chromatography on deae-sepharose and phenyl-sepharose resins, respectively. the enzyme had an apparent molecular weight of 29 kda, as determined by sds-page. the purified enzyme was most active at ph and temperature ranges of 4.5-6.5 and 55-60oc, respectively. in addition, x2 showed thermostability at 60oc with a half-life of approx. 5.5 h. the apparent km values, using soluble and insoluble arabinoxylans as substrates, were 10.87 and 11.20 mg/ml, respectively.
Production of xylan-degrading enzymes by a Trichoderma harzianum strain
Cacais, André O.Guerreiro;Silveira, Fabiane Q. de Paula;Ferreira Filho, Edivaldo Ximenes;
Brazilian Journal of Microbiology , 2001, DOI: 10.1590/S1517-83822001000200014
Abstract: trichoderma harzianum strain 4 produced extracellular xylan-degrading enzymes, namely b-xylanase, b-xylosidase and a-arabinofuranosidase, when grown in liquid medium cultures containing oat spelt xylan as inducer. cellulase activity was not detected. the pattern of xylan-degrading enzymes induction was influenced by the form of xylan present in the medium. they were detected in different incubation periods. electrophoretic separation of the proteins from liquid culture filtrates by sds-page showed a variety of bands with high and low molecular weights.
O trabalho em servi?os de saúde mental no contexto da reforma psiquiátrica: um desafio técnico, político e ético
Sampaio,José Jackson Coelho; Guimar?es,José Maria Ximenes; Carneiro,Cleide; Garcia Filho,Carlos;
Ciência & Saúde Coletiva , 2011, DOI: 10.1590/S1413-81232011001300017
Abstract: this paper deals with mental health taken within the context of brazilian psychiatric reform and profiling the transformations in the organization of work processes. this has occurred as a result of the advances with respect to the implementation of the services that replaced the classic psychiatric model and the reconfiguration of the scope of intervention and practices. from this standpoint, the paper seeks to pinpoint the contradictions and problems related to this process and its impact on the organization of work processes on the management of services and on worker health. lastly, strategies are prepared for the purpose of tackling the problem, chief among which are the following: the redefinition of spaces, practices, and the relationships among the different actors, namely managers, workers, and users; the adoption of co-management mechanisms; and clinical-institutional supervision.
Estudo da audi??o de crian?as de gestantes expostas ao ruído ocupacional: avalia??o por emiss?es otoacústicas - produto de distor??o
Rocha, Eduardo Bezerra;Azevedo, Marisa Frasson de;Ximenes Filho, Jo?o Arag?o;
Revista Brasileira de Otorrinolaringologia , 2007, DOI: 10.1590/S0034-72992007000300011
Abstract: aim: to detect early on a probable hearing loss in children of women exposed to occupational noise during their pregnancy and to verify if there is any difference between the children from those women exposed to occupational noise during their pregnancy and the ones from mothers that do not work under the same conditions. methods: children from women exposed to occupational noise during their pregnancy and children from women who were not exposed were evaluated through distortion product otoacoustic emissions, using the gsi 60 dpoea system equipment and the frequency-ratio f2/f1 equal to 1.2 and the geometric average of 2f1-f2. the intensity of the primary frequencies were kept steady with values of l1=65dbspl and l2=55dbspl for f1 and f2, respectively. student t test in paired samples and independent samples were used. results: there were no differences in the response amplitude of distortion product otoacoustic emissions between the control and the study groups. there was no statistically difference between male and female children in response amplitude for the two groups aforementioned; and there were no differences between right and left ears from each group. conclusion: we did not observe hearing impairment in children whose mothers were exposed to occupational noise during pregnancy when compared to the children from mothers who were not. there was no difference between the right and left ears, nor between male and female children in each group.
Residência médica em otorrinolaringologia no Ceará em 2003: oferta de vagas e perfil da concorrência
Ximenes Filho, Jo?o Arag?o;Silva, Marcelo Gurgel Carlos da;
Revista Brasileira de Otorrinolaringologia , 2006, DOI: 10.1590/S0034-72992006000600015
Abstract: aim: analyze and compare the openings for medical residency in otorhinolaryngology in ceará in 2003 along with the profile of the applicants. stud design: cross-sectional. methods: data on medical residency programs in ceará as of september 2003 were obtained electronically from the web page of the national commission of medical residency (cnrm). the information regarding the applicants was retrieved directly from the institutions offering medical residency programs in otorhinolaryngology in 2003 and complemented by searches in the databases of the ceará state regional council of medicine and the federal council of medicine. results: the total number of openings in otorhinolaryngology authorized by cnrm is 12. nine (75%) of the total and 4 (100%) of first year residence are currently filled. the hospital occupancy rate was 66.67% for hgf and 83.33% for huwc. competition per residency was 13.0 at both hospitals. the 48 applications received by the two hospitals were submitted by 37 doctors, 66.67% of whom were male. the largest number of candidates came from the medical schools of the federal universities of ceará (62.50%), paraíba (15.63%) and alagoas (9.38%). approximately 41% of the candidates had graduated in 2002. conclusion: this study presents a profile of medical residency in otorhinolaryngology.
Papilomatose laríngea recorrente: experiência de 10 anos
Ximenes Filho, Jo?o Arag?o;Simoceli, Lucinda;Imamura, Rui;Tsuji, Domingos Hiroshi;Sennes, Luiz Ubirajara;
Revista Brasileira de Otorrinolaringologia , 2003, DOI: 10.1590/S0034-72992003000500003
Abstract: introduction: airway papillomas are benign tumors with high recurrence and induced by virus. papillomatosis's etiology is related with papilloma human virus infection. two clinical forms are described: juvenile and adult. objective: the aim of this study was to compare the two clinical forms of the papillomatosis, juvenile and adult, observing if there are epidemiological or clinical differences between them. study design: historical cohort. material and method: we studied the patients followed in the ent department of hc-fmusp with diagnosis of laryngeal papilomatose between 1990 and 1999. fifty-one patients were identified, but seven did not confirm the diagnosis. thus, 44 individuals were the base of this study, being 47,72% of juvenile form and 52,27% of adult form. results: there was no prevalence between gender (p=.98). the mean age at the beginning of the symptoms was of 5,3 years in juvenile form and 42,6 years in adult form. dyspnea was more prevalent in juvenile form (p=.03). general recurrence was 66%, being of 76,2% in juvenile form and 56,5% in adult form (p=.17). the incidence of early recurrence (<3 months) was higher in juvenile form (p<.001). we observed 13% of malign transformation in adult form of the disease. conclusions: in juvenile form, we have identified early recurrences associated with dyspnea, requiring multiple interventions. in adult form, we have also observed elevated recurrences, with high index of malign transformation. treatments that increase the time between recurrences are necessary in two forms of papillomatosis.
Sympathetic overactivity and arrhythmias in tetanus: electrocardiographic analysis
Henriques Filho, Gustavo Trindade;Lacerda, Heloisa Ramos;Albuquerque, Afonso;Ximenes, Ricardo Arraes de Alencar;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2007, DOI: 10.1590/S0036-46652007000100004
Abstract: as a result of the advances in the control of pulmonary insufficiency in tetanus, the cardiovascular system has increasingly been shown to be a determining factor in morbidity and mortality but detailed knowledge of the cardiovascular complications in tetanus is scanty. the 24h-holter was carried out in order to detect arrhythmias and sympathetic overactivity in 38 tetanus patients admitted to an icu. the sdnn index (standard deviation from the normal r-to-r intervals), was useful in detecting adrenergic tonus, and ranged from 64.1 ± 27 in the more severe forms of tetanus to 125 ± 69 in the milder ones. sympathetic overactivity occurred in 86.2% of the more severe forms of the disease, but was also detected in 33% of the milder forms. half the patients had their sympathetic overactivity detected only by the holter. the most frequent arrhythmias were isolated supraventricular (55.2%) and ventricular (39.4%) extrasystoles. there was no association of the arrhythmias with the clinical form of tetanus or with the presence of sympathetic overactivity. the present study demonstrated that major cardiovascular dysfunction, particularly sympathetic overactivity, occurs in all forms of tetanus, even in the milder ones. this has not been effectively detected with traditional monitoring in icu and may not be properly treated.
Purification and characterization of a low molecular weight xylanase from solid-state cultures of Aspergillus fumigatus Fresenius
Silva, Claudio Henrique Cerri e;Puls, Jurgen;Sousa, Marcelo Valle de;Ferreira Filho, Edivaldo Ximenes;
Revista de Microbiologia , 1999, DOI: 10.1590/S0001-37141999000200005
Abstract: a xylan-degrading enzyme (xylanase ii) was purified to apparent homogeneity from solid-state cultures of aspergillus fumigatus fresenius. the molecular weight of xylanase ii was found to be 19 and 8.5 kda, as estimated by sds-page and gel filtration on fplc, respectively. the purified enzyme was most active at 55 °c and ph 5.5. it was specific to xylan. the apparent km and vmax values on soluble and insoluble xylans from oat spelt and birchwood showed that xylanase ii was most active on soluble birchwood xylan. studies on hydrolysis products of various xylans and xylooligomers by xylanase ii on hplc showed that the enzyme released a range of products from xylobiose to xylohexaose, with a small amount of xylose from xylooligomers, and presented transferase activity.
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