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Search Results: 1 - 10 of 28675 matches for " XiaoYun Sun "
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Medical Image Segmentation with Improved Gradient Vector Flow
Jinyong Cheng,Xiaoyun Sun
Research Journal of Applied Sciences, Engineering and Technology , 2012,
Abstract: In this study, we discover some deficiencies of GVF and GGVF Snake such as it can not capture boundaries like “U” and “Ω” completely because of the counteraction of some external forces and the influence of the local minimum external forces. Based on analyzing force distribution rules of gradient vector flow, a standard is introduced to distinguish every control point is true or false. An additional control force is added to GVF Snake model. The direction of control force is gained by tracking the force field and the motion of snake control points. Experimentation proves that the new GVF Snake model can solve the problem that GVF and GGVF Snake model can not detect the boundaries like “U” and “Ω” and the new algorithm can improve GVF snake model’s ability to capture thin boundary indentation like the boundary of brain image.
Probabilistic Analysis of Life Cycle Cost for Power Transformer  [PDF]
Jianpeng Bian, Xiaoyun Sun, Mingming Wang, Haiqing Zheng, Hui Xing
Journal of Power and Energy Engineering (JPEE) , 2014, DOI: 10.4236/jpee.2014.24066
Abstract: The selection of power transformer is very important to power sector. Most methods are utilized according to the initial cost and don’t consider the synthetical evaluation of economy and technology. Based on previous research, this paper addresses a new practical probabilistic life cycle cost model. Then, in order to demonstrate the practicability of probabilistic life cycle cost for the
power transformer, illustrative investment alternatives of actual power transformers are discussed. From the result of the numerical investigation, it may be positively stated that the optimum investment alternative for the power transformer based on the probabilistic life cycle cost model proposed in this study will lead to a more rational, economical and effective procedure compared with the conventional method only considering the initial cost.
Antibodies against outer-capsid proteins of grass carp reovirus expressed in E. coli are capable of neutralizing viral infectivity
Ling Shao, Xiaoyun Sun, Qin Fang
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-347
Abstract: Recombinant plasmids of GCRV outer capsid proteins VP5 and VP7 were constructed and expressed in prokaryotic cells in our previous work. In this study, we prepared GCRV Antibody (Ab), VP5Ab and VP7Ab generated from purified native GCRV, recombinant VP5 and VP7 respectively. Immunoblotting analysis showed that the prepared antibodies were specific to its antigens. In addition, combined plaque and cytopathic effect (CPE)-based TCID50 (50% tissue culture infective dose) assays showed that both VP5Ab and VP7Ab were capable of neutralizing viral infectivity. Particularly, the neutralizing activity of VP7Ab was 3 times higher than that of VP5Ab, suggesting that VP7 might be a dominating epitope. Moreover, the combination of VP5Ab and VP7Ab appeared to enhance GCRV neutralizing capacity.The results presented in this study indicated that VP7 protein was the major epitope of GCRV. Furthermore, VP5Ab and VP7Ab in combination presented an enhanced capacity to neutralize the GCRV particle, suggesting that the VP5 and VP7 proteins may cooperate with each other during virus cell entry. The data can be used not only to further define the surface epitope domain of GCRV but may also be applicable in the designing of vaccines.Grass carp reovirus (GCRV), a member of genus Aquareovirus in the family Reoviridae[1], was the first viral pathogen to be identified from aquatic animals in China; this virus was identified twenty years ago during an acute epidemic characterized by symptoms of hemorrhagic disease in fingerling and yearling grass carp [2,3]. In an attempt to control the spread of this disease, progress has been made using a crude inactive vaccine preparation, but the agent is far from being effective in the prevention of GCRV viral infection. In addition, GCRV has been recognized as the most pathogenic of the isolated aquareoviruses reported to date [4,5]. Therefore, it is important to develop an effective vaccine for better prevention and control of fatal outbreaks of hemorrhag
Radiative Forcing of SO2 and NOx: A Case Study in Beijing
Radiative Forcing of SO2 and NOx: A Case Study in Beijing

SUN Zhian,WANG Xiaoyun,ZENG Xianning,
SUN Zhian
,WANG Xiaoyun,ZENG Xianning

大气科学进展 , 2006,
Abstract: A case study was performed in Beijing in 2000 to observe concentrations of SO2 and NOx in the atmosphere and to evaluate their radiative impact. It was found that the concentrations of these gases are usually high in the morning due to a temperature inversion in the boundary layer. The average concentrations obtained from the observations are much higher than those used in the McClatchey reference atmosphere. The radiative impacts of these gases are calculated using a line-by-line radiative transfer model. The results show that the radiative forcing at the surface due to SO2 is 0.0576 W m-2 and that due to NOx is 0.0032 W m-2. These figures are almost compatible with that due to CFC11.
Astrocyte Proliferation Following Stroke in the Mouse Depends on Distance from the Infarct
George E. Barreto, Xiaoyun Sun, Lijun Xu, Rona G. Giffard
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0027881
Abstract: Reactive gliosis is a hallmark of brain pathology and the injury response, yet the extent to which astrocytes proliferate, and whether this is central to astrogliosis is still controversial. We determined the fraction of mature astrocytes that proliferate in a mouse stroke model using unbiased stereology as a function of distance from the infarct edge. Cumulatively 11.1±1.2% of Aldh1l1+ astrocytes within 400 μm in the cortical penumbra incorporate BrdU in the first week following stroke, while the overall number of astrocytes does not change. The number of astrocytes proliferating fell sharply with distance with more than half of all proliferating astrocytes found within 100 μm of the edge of the infarct. Despite extensive cell proliferation primarily of microglia and neutrophils/monocytes in the week following stroke, few mature astrocytes re-enter cell cycle, and these are concentrated close to the infarct boundary.
Characterization of grass carp reovirus minor core protein VP4
Liming Yan, Hong Guo, Xiaoyun Sun, Ling Shao, Qin Fang
Virology Journal , 2012, DOI: 10.1186/1743-422x-9-89
Abstract: To understand the role of minor core protein VP4 played in molecular pathogenesis during GCRV infection, the recombinant GCRV VP4 gene was constructed and expressed in both prokaryotic and mammalian cells in this investigation. The recombinant His-tag fusion VP4 products expressed in E.coli were identified by Western blotting utilizing His-tag specific monoclonal and GCRV polyclonal antibodies. In addition, the expression of VP4 in GCRV infected cells, appeared in granules structure concentrated mainly in the cytoplasm, can be detected by Immunofluorescence (IF) using prepared anti-VP4 polyclonal antibody. Meanwhile, VP4 protein in GCRV core and infected cell lysate was identified by Immunoblotting (IB) assay. Of particular note, the VP4 protein was exhibited a diffuse distribution in the cytoplasm and nucleus in transfected cells, suggesting that VP4 protein may play a partial role in the nucleus by regulating cell cycle besides its predicted cytoplasmic function in GCRV infection.Our results indicate the VP4 is a core component in GCRV. The cellular localization of VP4 is correlated with its predicted function. The data provide a foundation for further studies aimed at understanding the role of VP4 in viroplasmic inclusion bodies (VIB) formation during GCRV replication and assembly.Double stranded (ds) RNA viruses, which affect a very wide range of host species including vertebrates, invertebrates, plants, fungi, and prokaryotes, represent a diverse group of viral pathogens [1]. According to classification of the International Committee on Taxonomy of Viruses (ICTV), eight distinct families are currently recognized [2]. The Reoviridae, one of the most complex families in dsRNA group, consists of at least 15 distinct genera reported so far. The virus particles in the family of Reoviridae appear to be icosahedral in symmetry with an overall diameter of approximately 60–85 nm comprising 9–12 segmented dsRNA genome enclosed within multiple concentric protein capsids.
Functional investigation of grass carp reovirus nonstructural protein NS80
Lin Cai, Xiaoyun Sun, Ling Shao, Qin Fang
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-168
Abstract: As a step to understanding the basis of the part played by NS80 in GCRV replication and particle assembly, we used the yeast two-hybrid (Y2H) system to identify NS80 interactions with proteins NS38, VP4, and VP6 as well as NS80 and NS38 self-interactions, while no interactions appeared in the four protein pairs NS38-VP4, NS38-VP6, VP4-VP4, and VP4-VP6. Bioinformatic analyses of NS80 with its corresponding proteins were performed with all currently available homologous protein sequences in ARVs (avian reoviruses) and MRVs (mammalian reoviruses) to predict further potential functional domains of NS80 that are related to VFLS (viral factory-like structures) formation and other roles in viral replication. Two conserved regions spanning from aa (amino acid) residues of 388 to 433, and 562 to 580 were discovered in this study. The second conserved region with corresponding conserved residues Tyr565, His569, Cys571, Asn573, and Glu576 located between the two coiled-coils regions (aa ~513-550 and aa ~615-690) in carboxyl-proximal terminus were supposed to be essential to form VFLS, so that aa residues ranging from 513 to 742 of NS80 was inferred to be the smallest region that is necessary for forming VFLS. The function of the first conserved region including Ala395, Gly419, Asp421, Pro422, Leu438, and Leu443 residues is unclear, but one-third of the amino-terminal region might be species specific, dominating interactions with other viral components.Our results in this study together with those from previous investigations indicate the protein NS80 might play a central role in VFLS formation and viral components recruitment in GCRV particle assembly, similar to the μNS protein in ARVs and MRVs.Grass carp reovirus (GCRV) is a tentative member in the Aquareovirus genus of the Reoviridae family which shares a common genome of 9 to 12 double-stranded RNA (dsRNA) segments packaged within a multilayered icosahedral capsid shell [1]. Different from most identified aquareoviruses, G
Overexpression of Heat Shock Protein 72 Attenuates NF-κB Activation Using a Combination of Regulatory Mechanisms in Microglia
Patrick W. Sheppard ,Xiaoyun Sun ,Mustafa Khammash ?,Rona G. Giffard ?
PLOS Computational Biology , 2014, DOI: doi/10.1371/journal.pcbi.1003471
Abstract: Overexpression of the inducible heat shock protein 70, Hsp72, has broadly cytoprotective effects and improves outcome following stroke. A full understanding of how Hsp72 protects cells against injury is elusive, though several distinct mechanisms are implicated. One mechanism is its anti-inflammatory effects. We study the effects of Hsp72 overexpression on activation of the transcription factor NF-κB in microglia combining experimentation and mathematical modeling, using TNFα to stimulate a microglial cell line stably overexpressing Hsp72. We find that Hsp72 overexpression reduces the amount of NF-κB DNA binding activity, activity of the upstream kinase IKK, and amount of IκBα inhibitor phosphorylated following TNFα application. Simulations evaluating several proposed mechanisms suggest that inhibition of IKK activation is an essential component of its regulatory activities. Unexpectedly we find that Hsp72 overexpression reduces the initial amount of the RelA/p65 NF-κB subunit in cells, contributing to the attenuated response. Neither mechanism in isolation, however, is sufficient to attenuate the response, providing evidence that Hsp72 relies upon multiple mechanisms to attenuate NF-κB activation. An additional observation from our study is that the induced expression of IκBα is altered significantly in Hsp72 expressing cells. While the mechanism responsible for this observation is not known, it points to yet another means by which Hsp72 may alter the NF-κB response. This study illustrates the multi-faceted nature of Hsp72 regulation of NF-κB activation in microglia and offers further clues to a novel mechanism by which Hsp72 may protect cells against injury.
Skeletonema cf.costatum Biogenic Silica Production Rate Determinated by PDMPO Method Skeletonema cf.costatum Biogenic Silica Production Rate Determinated by PDMPO Method
ZHANG Guicheng,LENG Xiaoyun,FENG Yuanyuan,LI Xia,SUN Jun
- , 2017,
Abstract: Diatoms are the only ecological phytoplankton that require silicic acid for growth.They are also the dominant contributor of ocean's total primary productivity.Generation and circulation with silica walls,which the siliceous organisms form,is an important component of the marine biological pump.It is crucial to the study of the operational mechanisms of biological pump with different sea areas.Moreover,it is the key link to the study of global silicon cycle.This paper introduces the basic mechanism of the formation of diatom silica walls and a new way of researching silicic acid metabolism,namely the 2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)-methoxy)phenyl)oxazole(PDMPO) dyeing method.Under a fluorescence microscope after excitation with bright green fluorescence,it can combine with silicic acid to form a complex into the Si deposition within diatom cells.The advantage of this method is that it can monitor the metabolism of silicate after adding PDMPO.For experimentation and sample collection in each of the specified time points,samples were determinated through the unutilized silicic acid,silica dissoluble intracellular and Si deposition within diatom cells,not only using hot alkaline digestions method but also PDMPO dyeing method.Results showed a good linear relationship between PDMPO fluorescent value and biogenic silica concentration.It was also indicated that PDMPO had no deleterious impact on Skeletonema cf.costatum growth for 34 h and was useful for tracking newly-deposited biogenic silica in diatoms' frustules
Phytoplankton Species Composition of Four Ecological Provinces in Yellow Sea,China Phytoplankton Species Composition of Four Ecological Provinces in Yellow Sea,China
LI Xiaoqian,FENG Yuanyuan,LENG Xiaoyun,LIU Haijiao,SUN Jun
- , 2017,
Abstract: The ecological province based on phytoplankton species composition is important to understanding the interplay between environmental parameters and phytoplankton species composition. The aim of this study was to establish phytoplankton species composition ecological pattern thus elucidate the relationship between environmental factors and the phytoplankton species composition in the ecological provinces. Phytoplankton samples were collected from 31 stations in Yellow Sea(121.00?–125.00?E, 32.00?–39.22?N) in November 2014. The samples were enumerated and identified with the Uterm?hl method under an optical inverted microscope-AE2000 with magnifications of 200 × or 400 ×. In the present study, a total of 141 taxa belonging to 60 genera of 4 phyla of phytoplankton were identified, among them 101 species of 45 genera were Bacillariophyta, 36 species of 11 genera were Dinophyta, 3 species of 3 genera were Chrysophyta and 1 species of 1 genera was Chlorophyta. The study area was divided into 4 ecological provinces according to an unsupervised cluster algorithm applied to the phytoplankton biomass. A T-S(Temperature-Salinity) scatter diagram depicted with data of water temperature and salinity defined by environmental provinces matched well with the ecological provinces. The results of Canonical Correspondence Analysis(CCA) indicated that the phytoplankton species composition was mainly correlated with temperature, salinity and silicate concentration in the studied area. A method of establishing ecological provinces is useful to further understanding the environmental effects on the marine phytoplankton species composition and the consequent marine biogeochemistry
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