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Search Results: 1 - 10 of 71164 matches for " XU Mei-Ying "
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The Method Study of Shewanella decolorationis S12 Reduce Different Electron Acceptors with Anaerobic Fermentor Culture
脱色希瓦氏菌(Shewanella decolorationis) S12还原不同电子受体的厌氧发酵罐培养方法

WANG Bo,XU Mei-Ying,SUN Guo-Ping,
王博
,许玫英,孙国萍

微生物学通报 , 2010,
Abstract: Shewanella decolorationis S12 is a strain which can perform anaerobic respiration by using various electron acceptors under the anaerobic environment. In this study, to obtain the enough biomass and satisfy the need of scientific research such as membrane proteomics, we used the inorganic small molecule (sodium nitrate), metal ion (ferric citrate) and organic macromolecule (azo dye amaranth) as sole terminal electron acceptors, using different concentrations of electron donor and carbon source for anaerobic stationary culture and anaerobic fermentor culture of Shewanella decolorationis S12. The cells were cultured by fed-batch cultivation mode to confirm the optimal concentrations of electron donor and carbon source, finally the method of anaerobic fermentor culture for S12 was established. Comparing to the traditional anaerobic stationary culture method, anaerobic fermentor culture method not only ensured a high rate mass-transfer efficiency resulting in the effective reduction of electron acceptors, but also greatly increased the cell growth density, the max cell growth densities were increased to 325, 304, 369 times and cell growth times were decreased 26.5%, 17.6%, 7.5% separately. This method provided an effective way to culture a large number of cells and protein under anaerobic respiration condition. The procedure described above would be significance for the studies which need biomass cultivation of Shewanella genus bacteria and other anaerobic microorganisms under fully controlled conditions.
A Broad Spcetrum Decoloration Shewanella New Species--Shewanella cinica
一个降解染料的希瓦氏菌新种——中国希瓦氏菌

XU Mei-Ying,
许玫英
,郭俊,钟小燕,曹渭,孙国萍

微生物学报 , 2004,
Abstract: 从细胞形态、生理生化特性和16S rRNA基因和gyrB基因序列同源性分析等方面对一株广谱高效染料降解菌D14进行了鉴定.菌株D14的细胞壁革兰氏染色为阴性,细胞为杆状,大小为0.6μm~1.0μm×1.0μm~4.0μm,周身纤毛,极生单鞭毛,其生长pH范围为pH 7.0~10.0,最适生长pH为8.0,生长温度范围为4℃~40℃,最适生长温度为20℃~30℃.该菌株具有还原三价铁,液化明胶、Tween40和Tween 80,产生H2S的能力.在乳酸钠存在的条件下,能还原硝酸盐、亚硝酸盐、铁氧化物和硫代硫酸钠.可利用D-半乳糖、D-葡萄糖、蔗糖、丙酸钠、L-亮氨酸等多种有机物为碳源.BIOLOG细菌自动鉴定系统鉴定结果表明该菌株应归属于希瓦菌属.16S rDNA和gyrB基因序列分析结果表明,菌株D14与其亲缘关系最近的菌株Shewanella putrefacien ATCC8071T的16S rDNA序列相似值为97%(小于97.7%),gyrB基因序列相似值为87%(小于90%).菌体所含有的主要脂肪酸为iso-15:0,16:1ω7c,15:0和16:0,DNA中(G C)mol%含量为49.3.结合菌株D14的生理生化特征和分子生物学特性,将菌株定为希瓦氏菌属的一个新种,命名为中国希瓦氏菌(Shewanella cinica)D14T.
Culture medium optimization for laccase production by Coriolus hirsutus using molasses distillery wastewater
毛云芝菌利用糖蜜酒精废水产漆酶培养基优化

SUN Wei,XU Mei-Ying,SUN Guo-Ping,
孙巍
,许玫英,孙国萍

微生物学通报 , 2011,
Abstract: In order to enhance the production of laccase and reduce the cost of laccase production, the culture medium for the growth of Coriolus hirsutus, a laccase production strain, was studied using molasses distillery wastewater (MDW) as carbon source. The different concentrations of molasses distillery wastewater and proportion of urea were optimized consequently through Box-Behnken experiment design under the foundation of signal factor experiment. As the optimization result, the production of laccase with the concentration of MDW 47% and proportion of urea 0.5% was six times higher than that from the original medium used previously. This indicates that the laccase production using MDW as carbon source is feasible. At the same time, the study makes the theoretical foundation for the exploitation and utilization of MDW to produce high value product.
Determination of the Concentration of Biosurfactants Using a Surface Tension Curve Method
表面张力曲线法测定生物表面活性剂的浓度

LI An-Hu,SUN Guo-Ping,XU Mei-Ying,
李安华
,孙国萍,许玫英

微生物学通报 , 2010,
Abstract: In this study, we present the development of a new method for biosurfactants detection. A relation between surface tension and concentration of rhamnolipid was plotted in an x-y scatter by Origin using empirical formula. So the concentration of crude fermentation fluid was determined measuring surface tension of the diluted fermentation fluid with the fitted equation. This method is rapid, simple, accurate and low-cost compared to currently used methods.
GENETIC STRUCTURE AND SEQUENCE ANALYSIS OF FOUR POPULATIONS OF MYTILUS CORUSCUS IN THE COASTAL WATERS OF SOUTHEAST CHINA SEA USING 16S rRNA
我国东南沿海厚壳贻贝(Mytilus coruscus)4 个群体线粒体16S rRNA 序列及遗传结构分析

YE Ying-Ying,XU Mei-Ying,GUO Bao-Ying,WU Chang-Wen,
叶莹莹
,徐梅英,郭宝英,吴常文

海洋与湖沼 , 2012,
Abstract: 采用16S rRNA 基因测序技术, 对我国东南沿海4个地理群体的厚壳贻贝遗传结构及遗传变异进行研究。通过对4个厚壳贻贝群体共83个个体的线粒体16S rRNA 基因进行测序, 获得1个长度为305bp 的同源序列, 共检测到150个多态位点, 多态位点比例达49.18%。83个个体中共检测到28个单倍型, 单倍型多样性指数(Hd) 为0.810, 核苷酸多样性指数( Pi) 为0.09602, 平均核苷酸差异数( K ) 达27.846 。结果表明, 我国东南沿海厚壳贻贝群体具有较高的遗传多样性水平。遗传结构检测结果表明, 舟山群体、温州群体、宁德群体间的遗传距离小, 遗传分化系数( Fst) 为-0.0141 —0.0059之间, 群体内部无显著分化( P >0.05), 而福州群体与其它群体间遗传距离较大, 为0.215—0.217 之间, 遗传分化系数(Fst) 也较大, 为0.6217—0.6319 之间, 存在极显著的遗传分化( P <0.001)。
ISSR ANALYSIS ON GENETIC STRUCTURE OF FOUR MYTILUS CORUSCUS POPULATIONS
厚壳贻贝(Mytilus coruscus)4个群体遗传多样性的ISSR分析

YE Ying-Ying,XU Mei-Ying,GUO Bao-Ying,WU Chang-Wen,
叶莹莹
,徐梅英,郭宝英,吴常文

海洋与湖沼 , 2012,
Abstract: The genetic structure of four geographic populations of Mytilus coruscus along the coast of China was investigated by the inter-simple sequence repeat (ISSR) fingerprinting of 120 individual clams. Of the 192 ISSR loci tested, 188 (97.92%) were polymorphic with 13 ISSR primers. The genetic diversity was high at species level. The result showed that the PPL about four populations were among 81.77%—89.58%, H were among 0.2950—0.2818, I were among 0.4213—0.4447, respectively for the four populations with the lowest values in FZ population, the highest values in WZ population. The GST of the four populations was 0.1519, Nm was 3.0576—5.9714, AMOVA analysis showed that the genetic variation mainly from individuals within populations. Genetic distance and UPGMA tree showed that WZ was clustered with ND into one clad, then with ZS, and finally with FZ. The result showed that the further geographical distance, the higher genetic distance; the genetic distance is proportional to the geographical distance.
Dissimilatory Fe (Ⅲ) Reduction and its Applications in Contaminants Treatment
异化Fe(Ⅲ)还原及其在污染治理中的作用

XU Mei-Ying,GUO Jun,CEN Ying-Hua,SUN Guo-Ping,
许玫英
,郭俊,岑英华,孙国萍

微生物学通报 , 2007,
Abstract: 细菌的异化Fe(Ⅲ)还原指以Fe(Ⅲ)为末端电子受体在无氧条件下氧化有机物的产能过程,在生物地球化学循环中起着重要的作用。系统综述了异化Fe(Ⅲ)还原细菌与多种代谢反应相耦联的Fe(Ⅲ)还原过程、还原不溶性Fe(Ⅲ)氧化物的机制,及其与Fe(Ⅲ)还原相关的分子生物学的研究进展。介绍了国内外有关Fe(Ⅲ)还原在环境污染治理中的研究现状及其发展趋势。
Electricity Generated by Shewanella decolorationnis S12 in Microbial Fuel Cell
微生物燃料电池中脱色希瓦氏菌S12的产电特性研究

LI Deng-Lan,XU Mei-Ying,SUN Guo-Ping,
李登兰
,许玫英,孙国萍

微生物学通报 , 2008,
Abstract: In order to determine the electrochemical activity of Shewanella decolorationis S12, anaerobic grown cells were scanned by the cyclic voltammograms (CV). The results of CV showed that strain S12 displayed certain electrochemical activities and could be used as a catalyzer working in microbial fuel cell (MFC) to produce electricity. The effects of different electron donor, such as lactate, formate and pyruvate with different concentration on the production of electricity were analyzed by using strain S12 in a single chamber MFC with a PEM/electrode assembly sandwiched type. The results showed that lactate was the optimal electron donor and the concentration of the electron donor determined the electricity generation capacity and potential development in the MFC. When 10 mmol/L and 20 mmol/L of lactate were used, the maximum power density of 21.93 mW/m2 and 55.72 mW/m2 were obtained, respectively.
Over-expression of Highly Active Triphenylmethane Dyes Decolorization Enzyme (TpmD) Induced by Lactose Instead of IPTG in Escherichia coli BL21 (DE3)
乳糖替代IPTG诱导脱色酶TpmD基因在大肠杆菌中的高效表达

CHEN Liang,REN Sui-Zhou,XU Mei-Ying,SUN Guo-Ping,
陈 亮
,任随周,许玫英,孙国萍

微生物学通报 , 2009,
Abstract: 本文考察了乳糖代替IPTG诱导三苯基甲烷类染料脱色酶TpmD在大肠杆菌BL21(DE3)中表达的可行性, 分别对用乳糖作为诱导剂时的诱导时机、乳糖浓度、诱导持续时间和添加方式进行优化并与IPTG诱导的差异等方面进行了比较分析, 确定了乳糖诱导的最佳条件。结果表明, 在工程菌对数生长中期(OD600约为0.8)添加终浓度为0.4 mmol/L的乳糖诱导6 h的条件下能获得最大量的目的蛋白和菌体量。由于乳糖可以作为碳源被菌体利用, 分批添加乳糖效果优于一次性添加。乳糖诱导条件下目的蛋白表达量占总蛋白的35.62%, 与IPTG诱导条件下的35.03%无明显差异。乳糖诱导后外源蛋白的表达时间有所滞后, 但收获的菌体量高于IPTG诱导, 显示出了乳糖同样是一种T7启动子的廉价高效诱导剂, 可以代替昂贵的IPTG用于脱色酶TpmD的规模化发酵, 同时也为其他重组蛋白的生产提供了有益的参考和借鉴。
The Research Progress of Anaerobic Ammonium Oxidation in Wastewater Biotreatment
厌氧氨氧化在污水处理中的研究进展

LIAO Xiao-Bing,XU Mei-Ying,LUO Hui-Dong,SUN Guo-Ping,
廖小兵
,许玫英,罗慧东,孙国萍

微生物学通报 , 2010,
Abstract: Anaerobic ammonium oxidation (ANAMMOX) is a process that the bacteria of anammox convert NH4+-N into N2 with the NO2?-N as the primary electron acceptor under anaerobic condition. It is considered as a very promising nitrogen removal technology in future, because of its cost effectiveness and environmental friendly characteristics. Here, we review its mechanism, influence factors, application situation and the bacteria of anammox. Some suggestions are given for the development of anammox application in wastewater treatment.
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