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Methylation of phytohormones by the SABATH methyltransferases
LiJia Qu,Shuang Li,ShuFan Xing
Chinese Science Bulletin , 2010, DOI: 10.1007/s11434-010-3245-x
Abstract: In plants, one of the most common modifications of secondary metabolites is methylation catalyzed by various methyltransferases. Recently, a new class of methyltransferases, the SABATH family of methyltransferases, was found to modify phytohormones and other small molecules. The SABATH methyltransferases share little sequence similarity with other well characterized methyltransferases. Arabidopsis has 24 members of the SABATH methyltransferase genes, and a subset of them has been shown to catalyze the formation of methyl esters with phytohormones and other small molecules. Physiological and genetic analyses show that methylation of phytohormones plays important roles in regulating various biological processes in plants, including stress responses, leaf development, and seed maturation/germination. In this review, we focus on phytohormone methylation by the SABATH family methyltransferases and the implication of these modifications in plant development.
Lepton flavor violating signals of a little Higgs model at the high energy linear $e^{+}e^{-}$ colliders
Yue, Chong-Xing;Zhao, Shuang
High Energy Physics - Phenomenology , 2007, DOI: 10.1140/epjc/s10052-007-0234-6
Abstract: Littlest Higgs $(LH)$ model predicts the existence of the doubly charged scalars $\Phi^{\pm\pm}$, which generally have large flavor changing couplings to leptons. We calculate the contributions of $\Phi^{\pm\pm}$ to the lepton flavor violating $(LFV)$ processes $l_{i}\to l_{j}\gamma$ and $l_{i}\to l_{j}l_{k}l_{k}$, and compare our numerical results with the current experimental upper limits on these processes. We find that some of these processes can give severe constraints on the coupling constant $Y_{ij}$ and the mass parameter $M_{\Phi}$. Taking into account the constraints on these free parameters, we further discuss the possible lepton flavor violating signals of $\Phi^{\pm\pm}$ at the high energy linear $e^{+}e^{-}$ collider $(ILC)$ experiments. Our numerical results show that the possible signals of $\Phi^{\pm\pm}$ might be detected via the subprocesses $e^{\pm}e^{\pm}\to l^{\pm}l^{\pm}$ in the future $ILC$ experiments.
Identity Authentication and Context Privacy Preservation in Wireless Health Monitoring System
Qiming Huang,Xing Yang,Shuang Li
International Journal of Computer Network and Information Security , 2011,
Abstract: Privacy preservation is an important challenge for wireless health monitoring system. This paper analyses the privacy threat types based on the wireless health monitoring system architecture, and built the key system model for identity threat and context privacy preservation based on traffic analysis threat. To resist these threat , the integrated message encryption, identity authentication and traffic context privacy preservation, based on identity-based cryptography(IBC) and identity-based signature(IBS), is carried out at one time during the process of sending, receiving and accessing the patients’ health information. Extensive analysis demonstrates the effectiveness of the proposed scheme.
Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA
Feng,Tu; Liu,Shuang; He,Xing-jin;
Electronic Journal of Biotechnology , 2010,
Abstract: traditionally, the authentication of the traditional chinese medicines (tcm), angelica sinensis, is based on slightly different morphological characters and complex compounds. usually, those methods are simultaneously affected by several factors, leading to subtle and ambiguous results. in this study, the internal transcribed spacer (its) regions of a. sinensis and seven other angelica species used as adulterants were sequenced. a pair of specific primers was designed from the polymorphic its regions to distinguish a. sinensis from the adulterants and regional substitutes. these its-derived primers amplified approximately 520 bp specific fragments from the adulterants, whereas no products was amplified with the dna of a. sinensis. we tested eight commercially crude materials purchased in the market by using these specific primers. the result showed that there were four samples adulterating a. sinensis with regional substitutes. this indicated that a. sinensis could be accurately distinguished from the adulterants and regional substitutes. therefore, the method of molecular authentication based on the its sequences may be contributed to raw material production and quality control of a. sinensis.
Single production of the doubly charged scalar in the littlest Higgs model
Yue, Chong-Xing;Zhao, Shuang;Ma, Wei
High Energy Physics - Phenomenology , 2007, DOI: 10.1016/j.nuclphysb.2007.06.003
Abstract: Single production of the doubly charged scalars $\Phi^{\pm\pm}$ via $e\gamma$, $ep$ and $pp$ collisions is studied in the context of the little Higgs$(LH)$ model. Our numerical results show that the new particles $\Phi^{\pm\pm}$ can be abundantly produced and their possible signatures should be detected in future high energy linear $e^{+}e^{-}$ collider$(ILC)$. The cross section for single production of $\Phi^{\pm\pm}$ at the $LHC$ is much smaller than that at the $ILC$ or the $THERA$.
Reproduction of a hyperkalemia animal model
Jian HUO,Xing-li SU,Shuang WANG
Medical Journal of Chinese People's Liberation Army , 2011,
Abstract: Objective To reproduce an animal model of myocardium hyperkalemia and to relieve its symptoms with calcium chloride(CaCl2) with the purpose of observing the effect of hyperkalemia on electrocardiogram Ⅱ(ECGⅡ).Methods Fifty toads(either gender) were assigned into two groups,i.e.experiment group and control group.Animals in experiment group were injected with 0.3ml of 0.2% potassium chloride(KCl) via abdominal vein to reproduce hyperkalemia model,while those in control group were injected same amount of 0.9% sodium chloride(NaCl),then ECG Ⅱ was synchronously recorded.Two minutes later,animals in experiment group were injected with 0.2ml of 1% calcium chloride(CaCl2) via the abdominal vein to relieve hyperkalemia,and ECGⅡ was again synchronously recorded.Results After injection of 0.2% KCl,ECG Ⅱ of the animals in experiment group showed that the heart rate was slowed down(P < 0.05),P-R interval was extended(P < 0.05),QRS was broadened and lowered(P < 0.05),T peak became higher(P < 0.05) as compared with that in control group.Two minutes after injection of 1% CaCl2,the ECG Ⅱ of the animals in experiment group recovered to normal status,and no significant difference was found between that of experiment group and control group.Conclusion The changes in ECG Ⅱ in toad model of myocardium hyperkalemia,produced by injection of 0.2% KCl,and then relieve the symptoms by injection of 1% CaCl2 via abdominal vein,is similar to that in mammals.Compared with other animal models,the hyperkalemia toad model is more convenient to be established with practical and stable result,and the cost is lower.
Modeling Genome-Wide Dynamic Regulatory Network in Mouse Lungs with Influenza Infection Using High-Dimensional Ordinary Differential Equations
Shuang Wu, Zhi-Ping Liu, Xing Qiu, Hulin Wu
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0095276
Abstract: The immune response to viral infection is regulated by an intricate network of many genes and their products. The reverse engineering of gene regulatory networks (GRNs) using mathematical models from time course gene expression data collected after influenza infection is key to our understanding of the mechanisms involved in controlling influenza infection within a host. A five-step pipeline: detection of temporally differentially expressed genes, clustering genes into co-expressed modules, identification of network structure, parameter estimate refinement, and functional enrichment analysis, is developed for reconstructing high-dimensional dynamic GRNs from genome-wide time course gene expression data. Applying the pipeline to the time course gene expression data from influenza-infected mouse lungs, we have identified 20 distinct temporal expression patterns in the differentially expressed genes and constructed a module-based dynamic network using a linear ODE model. Both intra-module and inter-module annotations and regulatory relationships of our inferred network show some interesting findings and are highly consistent with existing knowledge about the immune response in mice after influenza infection. The proposed method is a computationally efficient, data-driven pipeline bridging experimental data, mathematical modeling, and statistical analysis. The application to the influenza infection data elucidates the potentials of our pipeline in providing valuable insights into systematic modeling of complicated biological processes.
Genetic diversity analysis of Morchella sp.by RAPD
Muhammad Irfan, Shuang Yang, Luo Yuxin, Jia-Xing Sun
Molecular Biology Research Communications , 2017, DOI: -
Abstract: This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymorphism in three species of morchella. Out of all eight primers tested in current study, one of them (B8) resulted 100% polymorphism among the three studied species. Based on the RAPD profile a similarity matrix was generated to construct a dendrogram for phylogenetic analysis revealing the relationship among the three species of morchella.
Molecular Cloning and Characterization of the First Caspase in the Striped Stem Borer, Chilo suppressalis
Ming-Xing Lu,Yu-Zhou Du,Shuang-Shuang Cao,Pingyang Liu,Jianyong Li
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms140510229
Abstract: Apoptosis is executed through the activity of the caspases that are aspartyl-specific proteases. In this study, we isolated the caspase gene ( Cscaspase-1) of Chilo suppressalis (one of the leading pests responsible for destruction of rice crops). It possesses the open reading frame (ORF) of 295 amino acids including prodomain, large subunit and small subunits, and two cleavage sites (Asp 23 and Asp 194) were found to be located among them. In addition to these profiles, Cscaspase-1 contains two active sites (His 134 and Cys 176). Genomic analysis demonstrated there was no intron in the genome of Cscaspase-1. The Cscaspase-1 transcripts were found in all tissues of the fifth instar larvae, and higher levels were found in the midgut, hindgut and Malpighian tubules. Examination of Cscaspase-1 expression in different developmental stages indicated low constitutive levels in the eggs and early larvae stages, and higher abundances were exhibited in the last larvae and pupae stages. The relative mRNA levels of Cscaspase-1 were induced by heat and cold temperatures. For example, the highest increase of Cscaspase-1 transcription was at ?3 °C and 36 °C respectively. In a word, Cscaspase-1 plays a role of effector in the apoptosis of C. suppressalis. It also correlates with development, metamorphosis and thermotolerance of C. suppreassalis.
Study of Core Competence of Logistics Cluster: The Integration and the Extension of Value Chain  [PDF]
Shuang Wang
American Journal of Industrial and Business Management (AJIBM) , 2015, DOI: 10.4236/ajibm.2015.51003
Abstract: This paper focuses on the study of core competence of logistics cluster with the solution in two levels: one is the integration of supply chain, and the other point is the extension of value chain; both of them are based on the measurement of agglomeration level of logistics cluster and association level of cluster external resources. Hereby the MAEI model is proposed which is used to evaluate the agglomeration and association level as well as to enhance the core competence of logistics cluster by the solution of integration and extension of value chain.
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