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Search Results: 1 - 10 of 26631 matches for " XIE Bao-Gui "
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ISOLATION AND OPTIMIZATION OF REGENERATION CONDITION OF TREMELLA FUCIFORMIS PROTOPLASTS
银耳原生质体分离与再生条件优化研究*

XIE Bao-Gui,ZHU Hu,
谢宝贵
,朱虎

菌物学报 , 2003,
Abstract: 应用正交设计,研究不同菌株(Tr01、Tr21)、材料(芽孢、菌丝体、子实体)、溶壁酶浓度和酶解温度对原生质体产量的影响。实验结果表明,实验材料对原生质体产量影响最大,以芽孢为材料原生质体产量可达到2.75×107个/ml,而菌丝体和子实体的原生质体产量仅为2.5×106个/ml 和1.0×106个/ml;在35℃下酶解,原生质体产量高;溶壁酶浓度在1%~3%范围内对原生质体产量影响不大;不同菌株原生质体产量差异不显著。本实验还研究了稳渗剂浓度对原生质体再生率的影响,结果表明,0.5 mol/L~0.7 mol/L的KCl 对原生质体再生没有显著差异,再生率最高可达32.3%。
Some New Taxa of Charophyta from Guangxi
广西轮藻的新分类群

Chen Wei-Qun,Hah Fu-Shan,Xie Bao-Gui,
陈维群
,韩福山,谢宝贵

中国科学院研究生院学报 , 1990,
Abstract: Guangxi, Situated in the southern border area of China, is extremely rich in Ch- arophytes. In this paper, however, reported are only new species and new varieties. Among the new taxa, Nitella haplodactyla has only one dactyl on the fertile branchlet, N. brachyclema and N. rarissima are provided with more than 4-celled dactyls, the fertile branchlet of N. pseudofl- abellata var. tetradynana has tetradynamous rays, and Chara piniformis is characterized by thehaplostichous cortex, long spinecells, and rudimentary stipulodes.
FRUIT-BODY COLOR INHERITANCE OF FLAMMULINA VELUTIPES
金针菇子实体颜色的遗传规律研究

XIE Bao-Gui,JIANG Yu-Ji,WU Wen-Li,
谢宝贵
,江玉姬,吴文礼

菌物学报 , 2004,
Abstract: The yellow strain F19 and white strain F8801 of Flammulina velutipes were used as parents in the present study. Monokaryotic strains were obtained by means of protoplast monokaryolization. Monokaryotic strains from both parents were paired and four F1 hybrid strains were obtained. Single spore strains were isolated from F1 fruit-body and they were paired with monokaryotic strains by back cross. F2 strains were cultivated and its fruit-body color was recorded for inheritance analysis. The results showed that yellow was dominant, and white recessive, suggesting fruit-body color was controlled by one allele (Cc) and the allele did not link with incompatibility factor gene either A or B.
Population differentiation of Mycogone perniciosa in Fujian, China
福建省有害疣孢霉菌Mycogone perniciosa的种群分化初探

WEN Zhi-Qiang,WANG Yu-Xi,LIU Xin-Rui,XIE Bao-Gui,
温志强
,王玉霞,刘新锐,谢宝贵

菌物学报 , 2010,
Abstract: Morphological characteristics of colonies and chlamydospores of 16 strains of Mycogone perniciosa isolated from cultivated Agaricus bisporus pilei in different counties of Fujian Province were observed, and ISSR molecular fingerprinting markers were employed to analyse the genetic diversity of these strains. It was indicated that the morphological characteristics of colonies and chlamydospores of these strains could be classified into 3 categories. The results of ISSR analysis also showed that these strains could be divided into three cladogram, corresponding to the conclusion of morphological characteristics. It was primarily believed that there were three populations of Mycogone perniciosa in Fujian Province.
Cloning, structural analyses and expression levels of phosphofructokinase gene in different strains of Volvariella volvacea
草菇磷酸果糖激酶(PFK)基因克隆、结构及不同菌株中表达量分析

LIU Peng-Hu,XIE Bao-Gui,DENG You-Jin,JIANG Yu-Ji,
刘朋虎
,谢宝贵,邓优锦,江玉姬

菌物学报 , 2013,
Abstract:
Comparative study of digestive enzyme activities in earthworms belonging to two distinct ecological categories
两种生态类型蚯蚓几种消化酶活性比较研究

ZHANG Bao-Gui,
张宝贵

生态学报 , 2001,
Abstract: Earthworms play an important part in transformation of organic residues and soil nutrient cycling.In order to determine the diets and the capacity of degrading organic substances,cellulase,protease acid and alkaline phosphatase activities were measured in the gut of an epigeic earthworm Eisenia fetida and an anecic earthworm Pheretima guillemi. Activity of protease,phosphatases and rate of CO 2 evolution were compared between casts of Pheretima guillemi and uningested soil.Cellulase activity was far more important in the gut of Eisenia fetida than that of Pheretima guillemi, while the activities of protease,acid and alkaline phosphatases were significantly lower that these of Pheretima guillemi .The differences of digestive enzyme activities between the two earthworm species may be resulted from the fact that Eisenia fetida feed on plant residues,while Pheretima guillemi consumes micro\|organisms on the organic debris.According to the results of the present study,it is recommended that in culture of Pheretima, it is important to increase microbial biomass in the cultural medium.
Comparative analysis of proteomic profile at different development stages of Volvariella volvacea by iTRAQ-coupled 2D LC-MSMS
iTRAQ结合2D LC-MS/MS技术在草菇不同生长发育时期蛋白质组分析中的应用

LIU Jing-Yu,JIANG Yu-Ji,XIE Bao-Gui,CHEN Bing-Zhi,LIAO Wei,DENG You-Jin,
刘靖宇
,江玉姬,谢宝贵,陈炳智,廖伟,邓优锦

微生物学通报 , 2012,
Abstract: Objective] This work aims to investigate differential expression proteins at different development stages of Volvariella volvacea using isobaric tags for relative and absolute quantification (iTRAQ)-coupled two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS) proteomics approach. Methods] Firstly, the proteins extracted from V. volvacea at different development stages were analyzed by SDS-PAGE. Secondly, the tandem mass spcetrometry data obtained from 2D LC-MS/MS were used to search using MASCOT search engine. After that, principal component analysis, hierarchical clustering and Gene Ontology were used to analyze the detected results. Results] Results showed that 1 039 protein groups, included a total of 2 335 unique peptides, were identified. Among them, 1 030 protein groups were provided quantitative information. Contrast to mycelia, 64 up-regulated and 150 down-regulated significantly differential proteins were found in fruiting bodies at different development stages of V. volvacea. Bioinformatics analysis revealed that iTRAQ-coupled 2D LC-MS/MS was a unique method for isolating and identifying protein groups of V. volvacea at different development stages. Conclusion] It is helpful to insight into the molecular mechanism of the fruiting body formation and development of V. volvacea and other macro-basidiomycetes in the future.
Isolation and identification of a bacterial strain JS018 capable of degrading several kinds of organophosphate pesticides
一株能高效降解几种有机磷农药的菌株JS018的鉴定

JIANG Yu-ji,DENG You-jin,LIU Xin-rui,XIE Bao-gui,HU Fang-ping,
江玉姬
,邓优锦,刘新锐,谢宝贵,胡方平

微生物学报 , 2006,
Abstract: Organophosphate pesticides are used widely all over the world and play an important role in plant pest control. However these pesticides are considered as pollutants and harmful to human health. To search for microorganisms that can degrade organophosphate pesticides with high efficiency, a bacterial strain, coded as JS018, was isolated and screened from the soil in the vicinity of Shanming Pesticides Factory, Shanming, Fujian. Laboratory tests showed that the bacterium could degrade several kinds of organophosphate pesticides, such as Parathion-methyl and phoxin. The strain's degrading rates on phoxin, Parathion-methyl, hostathion and dichlorvos in LB liquid fermentation medium for 36 h were 99%, 96%, 80.4% and 69.0% respectively. The bacterial colonies on LB plate appeared shiny and pale-pink in color. The bacteria were Gram-negative coccoids, 0.5 - 0.7 microm in diameter. They grew well at 30 - 38 degrees C and pH 7.0 - 9.0. The optimal temperature and pH for cell growth was 32 degrees C and pH 7.5 - 8.0, respectively. They did not grow in medium containing 6% or more NaCl. The antibiotic susceptibility tests showed that the strain was resistant to ampicillin, penicillin and lincomycin. It was sensitive to kanamycin, tetracycline and gentamicin. Laboratory tests also showed that the strain could ferment D-glucose, trehalose, melezitose and ethanol. It was negative in the production of indole and hydrogen sulfide. It could not liquefy gelatin, utilize citrate, nor ferment L-arabinose, sucrose, D-mannitol, D-xylose, fructose, D-galactose, maltose or lactose. The catalase, urease and nitrate reduction were positive. Based on its morphological, physiological and biochemical properties as well as the 16S rDNA sequence analysis result, the strain was tentatively identified as Roseomonas sp.
DDRT-PCR analysis of the substrate-decomposing capability of degenerate strains of Agaricus bisporus
双孢蘑菇分解基质能力退化的DDRT-PCR分析

CHEN Mei-Yuan,WANG Ze-Sheng,LI Hong-Rong,LIAO Jian-Hua,XIE Bao-Gui,
陈美元
,王泽生,李洪荣,廖剑华,谢宝贵

菌物学报 , 2010,
Abstract: 对4种不同液体培养基培养的双孢蘑菇Agaricus bisporus CGMCC No. 0214及其分解基质能力退化菌株0214-3、0214-5进行了24对引物组合的mRNA差异显示(DDRT-PCR)分析,结果发现8对引物组合扩增出可重复的差异,并克隆了9条差异片段。序列分析结果显示9条差异片段代表了5个基因的差异,它们与不同生物的ATP结合盒式运输亚家族A某蛋白、碳水化合物酯酶家族4蛋白、几丁质脱乙酰基酶、乙酰木聚糖酯酶II及其他一些未知蛋白具有较高的同源性。本研究结果为进一步进行双孢蘑菇分解基质能力退化的分子机理研究奠定了良好的基础。
The application of the ''bidirectional haploid nuclei migration'' in breeding and genetics of Lentinula edodes
双向核迁移在香菇遗传和育种中的应用研究

LIU Jing-Yu,LIU Xin-Rui,DENG You-Jin,LIU Fang,XIE Bao-Gui,
刘靖宇
,刘新锐,邓优锦,刘芳,谢宝贵

菌物学报 , 2011,
Abstract: Monokaryon mating tests between different strains of Lentinula edodes were carried out,and two pair dikaryotic strains were obtained by utilizing the mechanism of 'bidirectional haploid nuclei migration'.The strains were purified by isolation of single hyphal tips of dikaryotic strains and making use of 'Buller phenomenon'.Two pairs of monokaryotic and dikaryotic strains were analyzed by observations of colony shapes,microscopic examination,fruiting tests,and molecular markers.The results showed that the st...
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