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Search Results: 1 - 10 of 443124 matches for " Wu M "
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Development of a simple and powerful method, cDNA AFLP-SSPAG, for cloning of differentially expressed genes
M Wu
African Journal of Biotechnology , 2006,
Abstract: A novel plant differentially expressed gene cloning technique called cDNA-AFLP-SSPAG was descrybed. Using this method, Differential mRNA expression was found between high and low heterosis groups of maize. Differential cDNAs were easily obtained from silver stained cDNA-AFLP separated on polyacylamide gels. The cDNA was then reamplified, cloned and fragments were sequenced. Sequenced clones were used as probes in northern dot blot analyses and library screening. Full-length cDNA was cloned from a library suggesting that the cDNA-AFLP technique combined with silverstaining polyacrylamide gels provide a novel and very powerful gene cloning method.
Corrigendum
Wu Q, Chu M
International Journal of Nanomedicine , 2012, DOI: http://dx.doi.org/10.2147/IJN.S35773
Abstract: Corrigendum Corrigendum (1575) Total Article Views Authors: Wu Q, Chu M Published Date August 2012 Volume 2012:7 Pages 4531 - 4532 DOI: http://dx.doi.org/10.2147/IJN.S35773 Received: 09 July 2012 Accepted: 09 July 2012 Published: 15 August 2012 Wu Q, Chu M. Self-illuminating quantum dots for highly sensitive in vivo real-time luminescent mapping of sentinel lymph nodes. Int J Nanomedicine. 2012;7:3433–3443. The following sentence on page 3433 was incorrect in the published paper: "Here, we report on the first use of self-illuminating QDs for mouse axillary SLN mapping by bioluminescence resonance energy transfer, which was found to overcome these limitations." The correct sentence should read: "Here, we report on the use of self-illuminating 3-mercaptopropionic acid-capped CdTe/CdS QDs for mouse axillary SLN mapping by bioluminescence resonance energy transfer, which was found to overcome these limitations." Read the original article Post to: Cannotea Citeulike Del.icio.us Facebook LinkedIn Twitter Other articles by Dr Maoquan Chu A gold nanoshell with a silica inner shell synthesized using liposome templates for doxorubicin loading and near-infrared photothermal therapy Near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping Self-illuminating quantum dots for highly sensitive in vivo real-time luminescent mapping of sentinel lymph nodes Readers of this article also read: Inflammatory mediators: Parallels between cancer biology and stem cell therapy Wine consumers’ environmental knowledge and attitudes: Influence on willingness to purchase Anesthesiologists’ perception of patients’ anxiety under regional anesthesia Amino acid management of Parkinson’s disease: a case study Intercellular cancer collisions generate an ejected crystal comet tail effect with fractal interface embryoid body reassembly transformation Dexmedetomidine sedation in painful posterior segment surgery Post intrastromal corneal ring segments insertion complicated by Candida parapsilosis keratitis Cancer stem cell theory: therapeutic implications for nanomedicine What are the roles of carers in decision-making for amyotrophic lateral sclerosis multidisciplinary care? Oral biofilms: molecular analysis, challenges, and future prospects in dental diagnostics
Self-illuminating quantum dots for highly sensitive in vivo real-time luminescent mapping of sentinel lymph nodes
Wu Q, Chu M
International Journal of Nanomedicine , 2012, DOI: http://dx.doi.org/10.2147/IJN.S30709
Abstract: minating quantum dots for highly sensitive in vivo real-time luminescent mapping of sentinel lymph nodes Original Research (2717) Total Article Views Authors: Wu Q, Chu M Published Date July 2012 Volume 2012:7 Pages 3433 - 3443 DOI: http://dx.doi.org/10.2147/IJN.S30709 Received: 09 February 2012 Accepted: 17 April 2012 Published: 05 July 2012 Qiang Wu, Maoquan Chu School of Life Science and Technology, Tongji University, Shanghai, People's Republic of China Abstract: Quantum dots (QDs) show promise as novel nanomaterials for sentinel lymph node (SLN) mapping through their use in noninvasive in vivo fluorescence imaging, and they have provided remarkable results. However, in vivo fluorescence imaging has limitations mainly reflected in the strong autofluorescence and low deepness of tissue penetration associated with this technique. Here, we report on the first use of self-illuminating QDs for mouse axillary SLN mapping by bioluminescence resonance energy transfer, which was found to overcome these limitations. We used CdTe/CdS QDs synthesized in aqueous solution to conjugate a mutant of the bioluminescent protein, Renilla reniformis luciferase. The nanobioconjugates obtained had an average hydrodynamic diameter of 19 nm, and their luminescence catalyzed by the substrate (coelenterazine) could penetrate into at least 20 mm of hairless pigskin, which could be observed using an in vivo imaging system equipped with a 700 nm emission filter. Conversely, the fluorescence of the nanobioconjugates penetrated no more than 10 mm of pigskin and was observed with a strong background. When 80 μL of the nanobioconjugates (containing about 0.5 μmol/L of QDs) and 5 μL of coelenterazine (1 μg/μL) were intradermally injected into a mouse paw, the axillary SLN could be imaged in real time without external excitation, and little background interference was detected. Furthermore, the decayed luminescence of QD-Luc8 in SLNs could be recovered after being intradermally reinjected with the coelenterazine. Our data showed that using self-illuminating QDs, as opposed to fluorescence QDs, has greatly enhanced sensitivity in SLN mapping, and that the SLN could be identified synchronously by the luminescence and fluorescence of the self-illuminating QDs.
Nonlocal Effects of Chemical Substances on the Brain Produced through Quantum Entanglement
Hu H.,Wu M.
Progress in Physics , 2006,
Abstract: Photons are intrinsically quantum objects and natural long-distance carriers of information. Since brain functions involve information and many experiments have shown that quantum entanglement is physically real, we have contemplated from the perspective of our recent hypothesis on the possibility of entangling the quantum entities inside the brain with those in an external chemical substance and carried out experiments toward that end. Here we report that applying magnetic pulses to the brain when an anesthetic or pain medication was placed in between caused the brain to feel the effect of the said substance for several hours after the treatment as if the test subject had actually inhaled the same. The said effect is consistently reproducible. We further found that drinking water exposed to magnetic pulses, laser light or microwave when a chemical substance was placed in between also causes consistently reproducible brain effects in various degrees. Further, through additional experiments we have verified that the said brain effect is the consequence of quantum entanglement between quantum entities inside the brain and those of the chemical substance under study, induced by the photons of the magnetic pulses or applied lights. We suggest that the said quantum entities inside the brain are nuclear and/or electron spins and discuss the profound implications of these results.
The "Southwest Effect" Revisited: An Empirical Analysis of the Effects of Southwest Airlines and JetBlue Airways on Incumbent Airlines from 1993 to 2009
Steven M. Wu
Michigan Journal of Business , 2012,
Abstract: The expansion of Southwest Airlines and JetBlue Airways has sparked new empirical interest in the effects of low-cost carriers (LCC) on existing airfares. Namely, empirical studies have attempted to capture the threat, or potential competition, of an entrant. This paper examines incumbent airline prices as a result of potential and actual competition from both Southwest Airlines and JetBlue Airways from 1993 to 2009 by analyzing mean airfares as well as price dispersion on incumbent routes. I incorporate a panel OLS with fixed effects model as well as GLS model with random effects. Consistent with re-cent literature, this paper finds that legacy incumbents cut fares significantly when threatened by Southwest Airlines. However, low-cost incumbents do not exhibit the same magnitude of pre-emptive price cutting. When threatened by JetBlue, neither legacy nor low-cost carriers cut fares significantly, suggest-ing that incumbents react differently when threatened by Southwest versus JetBlue. The evidence of increased price dispersion is mixed with price dis-persion decreasing on legacy carrier routes as a result of Southwest threat and entry but increasing on legacy carrier routes as a result of JetBlue threat and entry.
Equivalent Circuit Model for Designing of Jerusalem Cross-Based Artificial Magnetic Conductors
M. Hosseinipanah,Q. Wu
Radioengineering , 2009,
Abstract: This paper introduces an equivalent circuit model to estimate the resonant frequency and reflection phase of Jerusalem cross-based artificial magnetic conductor (JC-AMC) structures for waves normally incident on the structure. The JC-AMC structure is composed of an array of Jerusalem cross-shaped frequency selective surfaces (JC-FSSs) printed on a metal-backed dielectric slab without vias. This approach is based on the coupled microstrip lines and transmission line theories. Analytical expressions for the resonant frequency and reflection phase resulting from our model are thoroughly verified by full-wave simulations for different samples reported in the open literature. A comprehensive parametric study of the design parameters of JC-AMC structure and the influence of these parameters on the reflection phase properties are presented. This model can easily be extended to complex-shaped AMCs.
Inelastic deformation behavior of thermal barrier coatings exposed at a high-temperature environment
Arai M.,Wu X.
EPJ Web of Conferences , 2010, DOI: 10.1051/epjconf/20100626010
Abstract: Thermal barrier coatings (TBCs) are usually deposited onto the surface of the high-temperature component such as gas turbine, in order to protect it from a hightemperature environment. Coating stress generated by such a high-temperature brings serious damages in TBCs in service. For predicting numerically it, it is necessary to develop the constitutive equation suite to plasam-sprayed TBCs. Previous studies have made clear that the freestanding ceramic coat peeled from TBC coated substrate deforms nonlinearly with a mechanical loading, however the results there have been restricted to the test done using as-sprayed sample. In this study, effect of deposition parameter and high-temperature exposure condition on stress-strain curve of the freestanding ceramic coating sample was examined. The associated deformation process was discussed with the microstructure changes observed after performing a bending test for the exposed sample.
Observation of Anomalous Properties associated with the Low Temperature Structural Distortion in β-FeSe and Related Superconductorsa
Wu M. K.
EPJ Web of Conferences , 2012, DOI: 10.1051/epjconf/20122300009
Abstract: The discovery of Superconductivity in the tetragonal phase FeSe provides a unique platform for the detailed investigation of the correlation between the physical properties and crystal structure to better understand the possible origin of superconductivity in the new iron-based superconductors. We have carried out a series of properties characterizations by measuring magnetic susceptibility, Raman, NMR and femtosecond spectroscopy on single crystals and epitaxial thin films of the FeSe and Te-doped Fe(SeTe) samples. Our results show clearly the presence of anomalies in all the characterized properties at the temperature where a structural distortion from tetragonal to orthorhombic (or monoclinic) appears for all superconducting samples, but not in the non-superconducting ones. This structural distortion was observed not accompanied by a magnetic ordering as commonly occurs in the parent compounds of FeAs-based superconductors. All the observations suggest that the low temperature structural distortion is essential for the occurrence of superconductivity in the FeSe and related compounds. Details of the experimental results will be presented and discussed.
Erratum: "Kinetic Theory of Spin Coherence of Electrons in Semiconductors" [Journal of Superconductivity 14, 245 (2001)]
M. W. Wu
Physics , 2001,
Abstract: Two typos in the published paper are pointed out. Both are just typos and the calculations in that paper are based on the correct formulism.
Asymptotic Inference for the Weak Stationary Double AR(1) Model  [PDF]
Fang Chang, Augustine C. M. Wong, Yanyan Wu
Open Journal of Statistics (OJS) , 2012, DOI: 10.4236/ojs.2012.22016
Abstract: An AR(1) model with ARCH(1) error structure is known as the first-order double autoregressive (DAR(1)) model. In this paper, a conditional likelihood based method is proposed to obtain inference for the two scalar parameters of interest of the DAR(1) model. Theoretically, the proposed method has rate of convergence O(n-3/2). Applying the proposed method to a real-life data set shows that the results obtained by the proposed method can be quite different from the results obtained by the existing methods. Results from Monte Carlo simulation studies illustrate the supreme accuracy of the proposed method even when the sample size is small.
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