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Search Results: 1 - 3 of 3 matches for " Wisnik "
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Plástica e anonimato: modernidade e tradi??o em Lucio Costa e Mário de Andrade
Wisnik, Guilherme;
Novos Estudos - CEBRAP , 2007, DOI: 10.1590/S0101-33002007000300009
Abstract: there is an analogy between the uniform and patterned antiinvidualism of modern architecture and the anonymous and collective character of popular art. this perception, stated by mário de andrade in 1928, can be seen as a guideline for the equation "modernity-patrimony" conceived by lucio costa by the end of the 30's. however, the latter acknowledgement of the "legitimacy of the plastic intention", by costa, will gradually set apart their conception regarding the role of art in society, and, as a consequence, the meaning they attribute to the process of brazilian cultural formation.
Arquitetura arruinada
Guilherme Wisnik
Novos Estudos - CEBRAP , 2010, DOI: 10.1590/s0101-33002010000200012
The Formation of Persister Cells in Stationary-Phase Cultures of Escherichia Coli Is Associated with the Aggregation of Endogenous Proteins
Daria Leszczynska, Ewelina Matuszewska, Dorota Kuczynska-Wisnik, Beata Furmanek-Blaszk, Ewa Laskowska
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0054737
Abstract: Persister cells (persisters) are transiently tolerant to antibiotics and usually constitute a small part of bacterial populations. Persisters remain dormant but are able to re-grow after antibiotic treatment. In this study we found that the frequency of persisters correlated to the level of protein aggregates accumulated in E. coli stationary-phase cultures. When 3-(N-morpholino) propanesulfonic acid or an osmolyte (trehalose, betaine, glycerol or glucose) were added to the growth medium at low concentrations, proteins were prevented from aggregation and persister formation was inhibited. On the other hand, acetate or high concentrations of osmolytes enhanced protein aggregation and the generation of persisters. We demonstrated that in the E. coli stationary-phase cultures supplemented with MOPS or a selected osmolyte, the level of protein aggregates and persister frequency were not correlated with such physiological parameters as the extent of protein oxidation, culturability, ATP level or membrane integrity. The results described here may help to understand the mechanisms underlying persister formation.
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