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Search Results: 1 - 10 of 4660 matches for " Willian Roger Zaika "
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Desenvolvimento de geleia de tamarillo contendo polpa integral Development of tamarillo jam containing whole pulp
Paulo Ribas Guilherme,Cláudio César Pessatto,Willian Roger Zaika,Ernesto Quast
Brazilian Journal of Food Technology , 2012,
Abstract: O tamarillo (Cyphomandra betacea Sendt) é originário da Regi o Andina do Peru. O objetivo deste trabalho foi desenvolver o produto 'geleia de tamarillo' e consistiu em: caracterizar os frutos e o seu rendimento em polpa; apresentar um protocolo de processo, e avaliar sensorialmente os produtos obtidos a partir de três formula es, diferenciadas quanto ao grau °Brix, ao tipo de a úcar e ao teor de pectina. Os frutos tamarillos apresentaram 54,8 ± 4,0 mm, 34,6 ± 2,4 mm e 36,2 ± 6,5 g de comprimento, diametro e massa, respectivamente. A polpa integral, de sabor doce e levemente ácida, apresentou 13,30 ± 0,14 °Brix e pH 4,19 ± 0,11. Os frutos renderam 83,3% de polpa integral e 51,5% de locular mucilaginosa, contendo sementes. As geleias de 50 °Brix e contendo glicose ou sacarose foram as mais aceitas pelos provadores quando comparadas à geleia de 40 °Brix, de menor concentra o de sólidos solúveis. Um porcentual maior ou igual a 70% do total de provadores aprovou as geleias de 50 °Brix para todos os seus atributos sensoriais avaliados e um mínimo de 40% manifestou inten o de compra. Considerando-se que a sacarose possui custo menor que a glicose, o protocolo de fabrica o da geleia de 50 °Brix contendo a sacarose e 2% de pectina foi o recomendado, na pesquisa, para a produ o de geleia de tamarillo. The Tamarillo (Cyphomandra betacea Sendt) is a fruit native to the Peruvian Andean region. The objective of this study was to develop the product 'tamarillo jam'. The study consisted of characterizing the fruits and their yield in pulp; presenting a protocol for the process; and carrying out a sensory analysis of three different formulations which differed according to their degree of °Brix, type of sugar and pectin content. The tamarillo fruits showed length (stem-apex), diameter and mass of 54.8 ± 4.0 mm, 34.6 ± 2.4 mm and 36.2 ± 6.5 g, respectively. The whole pulp had a sweet, slightly acid taste, with total soluble solids of 13.30 °Brix ± 0.14 and pH 4.19 ± 0.11. The fruits yielded 83.3% in whole pulp and 51.5% in mucilaginous pulp containing the seeds. Jams with 50 °Brix containing glucose or sucrose showed greater acceptance as compared to jams with 40 °Brix, containing less soluble solids. Over 70% of the panellists approved the jams with 50 °Brix for all the sensory attributes evaluated, and at least 40% expressed their intention to purchase it. Thus, considering the lower cost of sucrose as compared to glucose, the protocol for the production of tamarillo jam at 50 °Brix using sucrose and 2% of pectin was recommended.
Desenvolvimento de geleia de tamarillo contendo polpa integral
Guilherme, Paulo Ribas;Pessatto, Cláudio César;Zaika, Willian Roger;Quast, Ernesto;Quast, Leda Battestin;Ormenese, Rita de Cássia Salvucci Celeste;Raupp, Dorivaldo da Silva;
Brazilian Journal of Food Technology , 2012, DOI: 10.1590/S1981-67232012005000007
Abstract: the tamarillo (cyphomandra betacea sendt) is a fruit native to the peruvian andean region. the objective of this study was to develop the product 'tamarillo jam'. the study consisted of characterizing the fruits and their yield in pulp; presenting a protocol for the process; and carrying out a sensory analysis of three different formulations which differed according to their degree of °brix, type of sugar and pectin content. the tamarillo fruits showed length (stem-apex), diameter and mass of 54.8 ± 4.0 mm, 34.6 ± 2.4 mm and 36.2 ± 6.5 g, respectively. the whole pulp had a sweet, slightly acid taste, with total soluble solids of 13.30 °brix ± 0.14 and ph 4.19 ± 0.11. the fruits yielded 83.3% in whole pulp and 51.5% in mucilaginous pulp containing the seeds. jams with 50 °brix containing glucose or sucrose showed greater acceptance as compared to jams with 40 °brix, containing less soluble solids. over 70% of the panellists approved the jams with 50 °brix for all the sensory attributes evaluated, and at least 40% expressed their intention to purchase it. thus, considering the lower cost of sucrose as compared to glucose, the protocol for the production of tamarillo jam at 50 °brix using sucrose and 2% of pectin was recommended.
Purinergic Activation of Ca2+-Permeable TRPV4 Channels Is Essential for Mechano-Sensitivity in the Aldosterone-Sensitive Distal Nephron
Mykola Mamenko,Oleg Zaika,Min Jin,Roger G. O'Neil,Oleh Pochynyuk
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0022824
Abstract: Mechanical forces are known to induce increases of [Ca2+]i in the aldosterone-sensitive distal nephron (ASDN) cells to regulate epithelial transport. At the same time, mechanical stress stimulates ATP release from ASDN cells. In this study, we combined ratiometric Fura-2 based monitoring of [Ca2+]i in freshly isolated split-opened ASDN with targeted deletion of P2Y2 and TRPV4 in mice to probe a role for purinergic signaling in mediating mechano-sensitive responses in ASDN cells. ATP application causes a reproducible transient Ca2+ peak followed by a sustained plateau. Individual cells of the cortical collecting duct (CCD) and the connecting tubule (CNT) respond to purinergic stimulation with comparative elevations of [Ca2+]i. Furthermore, ATP-induced Ca2+-responses are nearly identical in both principal (AQP2-positive) and intercalated (AQP2-negative) cells as was confirmed using immunohistochemistry in split-opened ASDN. UTP application produces elevations of [Ca2+]i similar to that observed with ATP suggesting a dominant role of P2Y2-like receptors in generation of [Ca2+]i response. Indeed, genetic deletion of P2Y2 receptors decreases the magnitude of ATP-induced and UTP-induced Ca2+ responses by more than 70% and 90%, respectively. Both intracellular and extracellular sources of Ca2+ appeared to contribute to the generation of ATP-induced Ca2+ response in ASDN cells. Importantly, flow- and hypotonic-induced Ca2+ elevations are markedly blunted in P2Y2 ?/? mice. We further demonstrated that activation of mechano-sensitive TRPV4 channel plays a major role in the sustained [Ca2+]i elevation during purinergic stimulation. Consistent with this, ATP-induced Ca2+ plateau are dramatically attenuated in TRV4 ?/? mice. Inhibition of TRPC channels with 10 μM BTP2 also decreased ATP-induced Ca2+ plateau whilst to a lower degree than that observed with TRPV4 inhibition/genetic deletion. We conclude that stimulation of purinergic signaling by mechanical stimuli leads to activation of TRPV4 and, to a lesser extent, TRPCs channels, and this is an important component of mechano-sensitive response of the ASDN.
The formation of professional reliability of locomotive drivers
Viktar ZAIKA
Transport Problems : an International Scientific Journal , 2012,
Abstract: Any activity accustoms on the base of the general abilities which develop in this activity, and "special" abilities are the general abilities which have got lines of efficiency under the influence of requirements of activity. And the more technical is the activity, the bigger role in its realization is played by "special" abilities [8, 9]. Hence, definition of effective receptions of psychophysiological and psychological diagnostics should be combined with a finding of optimum ways of vocational training.
Emerging Role of the Calcium-Activated, Small Conductance, SK3 K+ Channel in Distal Tubule Function: Regulation by TRPV4
Jonathan Berrout, Mykola Mamenko, Oleg L. Zaika, Lihe Chen, Wenzheng Zang, Oleh Pochynyuk, Roger G. O'Neil
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0095149
Abstract: The Ca2+-activated, maxi-K (BK) K+ channel, with low Ca2+-binding affinity, is expressed in the distal tubule of the nephron and contributes to flow-dependent K+ secretion. In the present study we demonstrate that the Ca2+-activated, SK3 (KCa2.3) K+ channel, with high Ca2+-binding affinity, is also expressed in the mouse kidney (RT-PCR, immunoblots). Immunohistochemical evaluations using tubule specific markers demonstrate significant expression of SK3 in the distal tubule and the entire collecting duct system, including the connecting tubule (CNT) and cortical collecting duct (CCD). In CNT and CCD, main sites for K+ secretion, the highest levels of expression were along the apical (luminal) cell membranes, including for both principal cells (PCs) and intercalated cells (ICs), posturing the channel for Ca2+-dependent K+ secretion. Fluorescent assessment of cell membrane potential in native, split-opened CCD, demonstrated that selective activation of the Ca2+-permeable TRPV4 channel, thereby inducing Ca2+ influx and elevating intracellular Ca2+ levels, activated both the SK3 channel and the BK channel leading to hyperpolarization of the cell membrane. The hyperpolarization response was decreased to a similar extent by either inhibition of SK3 channel with the selective SK antagonist, apamin, or by inhibition of the BK channel with the selective antagonist, iberiotoxin (IbTX). Addition of both inhibitors produced a further depolarization, indicating cooperative effects of the two channels on Vm. It is concluded that SK3 is functionally expressed in the distal nephron and collecting ducts where induction of TRPV4-mediated Ca2+ influx, leading to elevated intracellular Ca2+ levels, activates this high Ca2+-affinity K+ channel. Further, with sites of expression localized to the apical cell membrane, especially in the CNT and CCD, SK3 is poised to be a key pathway for Ca2+-dependent regulation of membrane potential and K+ secretion.
Cooperative apoptosis of coelomocytes of the holothurian Eupentacta fraudatrix and its modulation by dexamethasone  [PDF]
Olga A. Zaika, Lyudmila S. Dolmatova
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.49119
Abstract:

The capacities of phagocytes of subpopulation P1 (F) and morula cells (MC) of holothurian Eupentacta fraudatrix to modulate apoptosis of each other as well as cytokine-dependent mechanisms and hormonal regulation of these cells’s interaction were studied. The 18-h treatment of F with supernatants, obtained after centrifugation of MC preincubated for 3 h with phosphate buffered saline (PSB) at the temperature of 22℃ (SMC3) resulted in a significant growth of apoptosis level. A 30-min incubation of F with supernatants of MC, preincubated for 24 h (SMC24), on the contrary, reduced the apoptosis level and increased the level of interleukine-1α (IL-1α)-like substances, and 24-h incubation did not influence apoptosis and reduced level of IL-1α-like substances. Thus, proapoptotic effects of MC’s supernatants in F inversely depended on time of their preincubation with PSB and directly on time of incubation with F. Additionally, this effect was opposite to variations in the level of IL-1α-like substances. The level of apoptosis declined after 30 min of incubation but elevated after 24 h at the inverse treatment of MC with supernatant, obtained after preincubation of F during 24 h (SF24). The level of IL-1α-like substances dropped after 30 min and insignificantly decreased after 24 h. Hence, SF24 proapoptotic effect directly depended on time of incubation with MC and did not correspond to varia

p53 Family: Role of Protein Isoforms in Human Cancer
Jinxiong Wei,Elena Zaika,Alexander Zaika
Journal of Nucleic Acids , 2012, DOI: 10.1155/2012/687359
Abstract: TP53, TP63, and TP73 genes comprise the p53 family. Each gene produces protein isoforms through multiple mechanisms including extensive alternative mRNA splicing. Accumulating evidence shows that these isoforms play a critical role in the regulation of many biological processes in normal cells. Their abnormal expression contributes to tumorigenesis and has a profound effect on tumor response to curative therapy. This paper is an overview of isoform diversity in the p53 family and its role in cancer. 1. Introduction Alternative splicing allows a single gene to express multiple protein variants. It is estimated that 92–95% of human multiexon genes undergo alternative splicing [1, 2]. Abnormal alterations of splicing may interfere with normal cellular homeostasis and lead to cancer development [3–5]. The p53 protein family is comprised of three transcription factors: p53, p63, and p73. Phylogenetic analysis revealed that this family originated from a p63/73-like ancestral gene early in metazoan evolution [6, 7]. Maintenance of genetic stability of germ cells seems to be its ancestral function [8]. The p53 family regulates many vital biological processes, including cell differentiation, proliferation, and cell death/apoptosis [9, 10]. Dysregulation of the p53 family plays a critical role in tumorigenesis and significantly affects tumor response to therapy. This review summarizes current data on the regulation of p53, p63, and p73 isoforms and their roles in cancer. 2. Structure and Function p53, p63, and p73 genes are located on chromosomes 17p13.1, 3q27-29, and 1p36.2-3, respectively. These genes encode proteins with similar domain structures and significant amino acid sequence homology in the transactivation, DNA-binding and oligomerization domains (Figure 1). The highest amino acid identity is in the DNA-binding domain (~60%). Evolutionally, this domain is the most conserved, suggesting that regulation of transcription plays a pivotal role in an array of functions attributed to the p53 family. Less similarity is found in the oligomerization and transactivation domains (~30%). Figure 1: Architectures of human TP53, TP73, and TP63 genes. (A) TP53, TP73, and TP63 genes encode the transactivation (TAD), DNA-binding (DBD), and oligomerization (OD) domains. TP73 and TP63 encode additional SAM (Sterile Alpha Motif) domain. Percentage homology of residues between p53, p63, and p73 is shown [ 11]. (B) TP53, TP63, and TP73 genes have two promoters (P1 and P2). The P1 promoters produce transactivation-competent full-length proteins (TA) while the P2 promoters
Integral observability operators of nonlinear dynamical systems
Yury V. Zaika
International Journal of Mathematics and Mathematical Sciences , 2003, DOI: 10.1155/s0161171203203252
Abstract: In terms of functional dependence, the description of observable functions in nonlinear dynamical systems, which are analytic with respect to phase variables, is obtained. For processing of measurements, integral operators are used, which provide certain noise stability of operation of phase state reconstruction. The analogue of the duality theory known for linear problems of observation and control is developed. Computing schemes for nonlinear observability problem are proposed.
Interval estimates of functionals in time-delay systems with uncertainty
Yury V. Zaika
International Journal of Mathematics and Mathematical Sciences , 2003, DOI: 10.1155/s0161171203203264
Abstract: We consider a linear dynamical system with delay and uncertainty in initial data and movement and measurement equations. We present an algorithm of estimating an interval of possible values of functionals on solutions. We construct suboptimal weight functions in integral observation operators to minimize a sure estimation.
Identification of a hydrogen transfer model with dynamical boundary conditions
Yury V. Zaika
International Journal of Mathematics and Mathematical Sciences , 2004, DOI: 10.1155/s0161171204203271
Abstract: The nonlinear inverse problem of determining the hydrogen permeability parameters of stratified materials is considered. The model includes diffusion, invertible capture and sorption/desorption processes on surface, which leads to dynamical boundary conditions. For the concentration impulses method, the equation of stationary oscillations of concentration is obtained. Parametric identification scheme of the model is proposed. Techniques of Fourier series and conjugate equations are used.
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