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Search Results: 1 - 10 of 104153 matches for " WenQian Zhang "
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Detection of Wormhole in Maize based on Kernel RGB Image and Lighting Transformation Method
Jiangbo Li,Wenqian Huang,Chi Zhang
Advance Journal of Food Science and Technology , 2013,
Abstract: To effectively extract wormhole areas in kernels of maize, the uneven intensity distribution that was produced by the lighting system or by part of the vision system in the image must be transformed. A methodology was developed to convert non-uniform intensity distribution on objects into a uniform intensity distribution. A basically plane image with the wormhole area having a lower gray level than this plane was obtained by using proposed algorithms. Then, the wormhole areas can be easily extracted by a global threshold value. The experimental results with a 99.0% classification rate based on 100 kernel images showed that the proposed algorithm was simple and effective.

Feng Shuchang,Wu Liqing,Zhang Wenqian,Chen Yongyi,

大气科学进展 , 1988,
Abstract: This article, on the basis of the expert’s analyses and theories of the forecast of heavy-rain, summarizes a variety of the current instruments and methods of forecast and, according to the train of thought of the highrank forecasters, distills their experiences in forecasting heavy-rain into an inference-tree of 106 junctures from which 101 rule-bases are derived. The logical calculation is automatically carried out with our introduced and developed PROLOG, one of the intelligent languages by means of micro-computer. This process adopts the uncertain inferential method based on the theory of fuzzy sets, breaks through the limits of two-value logic and is characteristic of the thinking of human brain.
Deciphering Heterogeneity in Pig Genome Assembly Sscrofa9 by Isochore and Isochore-Like Region Analyses
Wenqian Zhang,Wenwu Wu,Wenchao Lin,Pengfang Zhou,Li Dai,Yang Zhang,Jingfei Huang,Deli Zhang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0013303
Abstract: The isochore, a large DNA sequence with relatively small GC variance, is one of the most important structures in eukaryotic genomes. Although the isochore has been widely studied in humans and other species, little is known about its distribution in pigs.
Expression and Activation of STAT3 in the Astrocytes of Optic Nerve in a Rat Model of Transient Intraocular Hypertension
Shaodan Zhang, Weiyi Li, Wenqian Wang, Samuel S. Zhang, Ping Huang, Chun Zhang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0055683
Abstract: Lamina cribosa, an astrocyte-rich region, is the origin of axonal degeneration in glaucomatous neuropathy. Astrocytes are particularly activated during optic nerve (ON) degeneration and are likely to contribute to the pathogenesis of glaucomatous optic neuropathy. Signalling mechanisms that regulate different aspects of astrocyte reactiviation in response to intraocular hypertensive injury are not well defined. Signal transducer and activator of transcription protein-3 (STAT3) is a transcription factor that participates in many biological processes and has been implicated as activator of reactive astrogliosis. In this study, we investigated the role of STAT3 in regulating the activation of astrocytes to transient intraocular hypertension in vivo by using a rat ocular hypertension model. ON astrocytes hypertrophy was observed early after intraocular hypertensive stress. Morphological changes in glial fibrillary acidic protein (GFAP) positive cells coupled with axon loss in the optic nerve was detected at day 7 after the injury. Nestin was significantly upregulated in ON astrocytes as early as day 2 post injury and kept elevated through post injury day 7. Phosphorylated STAT3 (pSTAT3) was markedly upregulated in ON astrocytes at post injury day 1, prior to the reactivation of ON astrocytes. These findings indicate that STAT3 signalling is involved in the initiation of astrocyte reactivation in optic nerve injury.
TIPE2 Negatively Regulates Inflammation by Switching Arginine Metabolism from Nitric Oxide Synthase to Arginase
Yunwei Lou, Guizhong Zhang, Minghong Geng, Wenqian Zhang, Jian Cui, Suxia Liu
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0096508
Abstract: TIPE2, the tumor necrosis factor (TNF)-alpha-induced protein 8-like 2 (TNFAIP8L2), plays an essential role in maintaining immune homeostasis. It is highly expressed in macrophages and negatively regulates inflammation through inhibiting Toll-like receptor signaling. In this paper, we utilized RAW264.7 cells stably transfected with a TIPE2 expression plasmid, as well as TIPE2-deficient macrophages to study the roles of TIPE2 in LPS-induced nitric oxide (NO) and urea production. The results showed that TIPE2-deficiency significantly upregulated the levels of iNOS expression and NO production in LPS-stimulated macrophages, but decreased mRNA levels of arginase I and urea production. However, TIPE2 overexpression in macrophages was capable of downregulating protein levels of LPS-induced iNOS and NO, but generated greater levels of arginase I and urea production. Furthermore, TIPE2?/? mice had higher iNOS protein levels in lung and liver and higher plasma NO concentrations, but lower levels of liver arginase I compared to LPS-treated WT controls. Interestingly, significant increases in IκB degradation and phosphorylation of JNK, p38, and IκB were observed in TIPE2-deficient macrophages following LPS challenge. These results strongly suggest that TIPE2 plays an important role in shifting L-arginase metabolism from production of NO to urea, during host inflammatory response.
Vertebrate Paralogous MEF2 Genes: Origin, Conservation, and Evolution
Wenwu Wu,Stefan de Folter,Xia Shen,Wenqian Zhang,Shiheng Tao
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0017334
Abstract: The myocyte enhancer factor 2 (MEF2) gene family is broadly expressed during the development and maintenance of muscle cells. Although a great deal has been elucidated concerning MEF2 transcription factors' regulation of specific gene expression in diverse programs and adaptive responses, little is known about the origin and evolution of the four members of the MEF2 gene family in vertebrates.
Molecular cloning and expression analysis of a zebrafish novel zinc finger protein gene rnf141
Deng, Wenqian;Sun, Huaqin;Liu, Yunqiang;Tao, Dachang;Zhang, Sizhong;Ma, Yongxin;
Genetics and Molecular Biology , 2009, DOI: 10.1590/S1415-47572009005000062
Abstract: znf230 is a novel zinc finger gene cloned by our laboratory. in order to understand the potential functions of this gene in vertebrate development, we cloned the zebrafish orthologue of human znf230, named rnf141. the cdna fragment of rnf141 was obtained by rapid amplification of cdna ends (race). the open reading frame (orf) encodes a polypeptide of 222 amino acids which shares 75.65% identity with the human znf230. rt-pcr analysis in zebrafish embryo and adult tissues revealed that rnf141 transcripts are maternally derived and that rnf141 mrna has a broad distribution. zygotic rnf141 message is strongly localized in the central nervous system, as shown by whole-mount in situ hybridization. knockdown and over expression of rnf141 can induce abnormal phenotypes, including abnormal development of brain, as well as yolk sac and axis extendsion. marker gene analysis showed that rnf141 may play a role in normal dorsoventral patterning of zebrafish embryos, suggesting that rnf141 may have a broad function during early development of vertebrates.
Analysis of synonymous codon usage in Hepatitis A virus
Yiqiang Zhang, Yongsheng Liu, Wenqian Liu, Jianhua Zhou, Haotai Chen, Yin Wang, Lina Ma, Yaozhong Ding, Jie Zhang
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-174
Abstract: The overall extent of codon usage bias in HAV is high in Picornaviridae. And the patterns of synonymous codon usage are quite different in HAV genomes from different location. The base composition is closely correlated with codon usage bias. Furthermore, the most important determinant that results in such a high codon bias in HAV is mutation pressure rather than natural selection.HAV presents a higher codon usage bias than other members of Picornaviridae. Compositional constraint is a significant element that influences the variation of synonymous codon usage in HAV genome. Besides, mutation pressure is supposed to be the major factor shaping the hyperendemic codon usage pattern of HAV.Hepatitis A virus (HAV), the causative agent of type A viral hepatitis, is an ancient human virus that was first identified in the stools of infected people in 1973 [1]. HAV is a non-enveloped, single-stranded positive-sence RNA virus which belongs to order Picornavirales, family Picornaviridae, the genus Hepatovirus in virus taxonomy [2-4]. The genome of HAV is approximately 7500 nucleotide in length and contains a large open-reading frame (ORF) encoding a polyprotein in which the major capsid proteins represent the amino-terminal third, with the remainder of the polyprotein comprising a series of nonstructural proteins required for HAV RNA replication: 2B, 2C, 3A, 3B, 3Cpro and 3Dpol. Based on the studies of genetics, HAV was proposed to divide into six different genotypes [5]. However, there is only one known serological group of human HAV [6,7]. Although HAV causes occasional, dramatic disease outbreaks of acute hepatitis with fatal outcomes in otherwise healthy adults as well as isolated severe cases of hepatitis, it has never been associated with chronic liver disease [8].As we all know, the genetic code chooses 64 codons to represent 20 standard amino acids and stop signals. These alternative codons for the same amino acid are termed as synonymous codons. Synonymous mutations t
Molecular Characterization of RXR (Retinoid X Receptor) Gene Isoforms from the Bivalve Species Chlamys farreri
Jia Lv, Liying Feng, Zhenmin Bao, Huihui Guo, Yueyue Zhang, Wenqian Jiao, Lingling Zhang, Shi Wang, Yan He, Xiaoli Hu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0074290
Abstract: Background Bivalves are among the oldest classes of invertebrates, and they exhibit diverse types of sexual patterning. However, our current understanding of the mechanisms of sex determination and differentiation in bivalves remains very limited. The retinoid X receptors (RXRs), which are members of the nuclear receptor family, are involved in sex differentiation in many organisms. Results In the present study, four full-length RXR-encoding cDNAs (CfRXRs) named CfRXRa, CfRXRb, CfRXRc and CfRXRd were retrieved from Zhikong scallop (Chlamys farreri). The four RXRs exhibited the conserved five-domain structure of nuclear receptor superfamily members and differed from each other only in the T-box of the C domain. The three variants, designated T (+4), T (+20) and T (+24), contained insertions of 4, 20 and 24 amino acids, respectively. The entire CfRXR gene is composed of eight exons and seven introns, and the four isoforms are generated via alternative mRNA splicing. Expression analysis showed that all four isoforms were expressed in both the testis and the ovary during the differentiation stage, whereas no expression was detected in the growth, mature or resting stages. This result suggests that CfRXRs are involved in germ cell differentiation in both sexes. The expression of the four isoforms was also detected in other tissues examined, including mantle, gill, digestive gland, and adductor muscle of sexually mature male and female Zhikong scallops, implying the multiple biological functions of CfRXRs. Conclusion Our study presents the first report of RXR isoforms in bivalves. Further investigation of the functional roles of different RXR isoforms may provide deep insights into the regulatory mechanism of sex differentiation in C. farreri.
Reference-free SNP calling: improved accuracy by preventing incorrect calls from repetitive genomic regions
Jinzhuang Dou, Xiqiang Zhao, Xiaoteng Fu, Wenqian Jiao, Nannan Wang, Lingling Zhang, Xiaoli Hu, Shi Wang, Zhenmin Bao
Biology Direct , 2012, DOI: 10.1186/1745-6150-7-17
Abstract: Here we describe an improved maximum likelihood (ML) algorithm called iML, which can achieve high genotyping accuracy for SNP calling in the non-model organisms without a reference genome. The iML algorithm incorporates the mixed Poisson/normal model to detect composite read clusters and can efficiently prevent incorrect SNP calls resulting from repetitive genomic regions. Through analysis of simulation and real sequencing datasets, we demonstrate that in comparison with ML or a threshold approach, iML can remarkably improve the accuracy of de novo SNP genotyping and is especially powerful for the reference-free genotyping in diploid genomes with high repeat contents.The iML algorithm can efficiently prevent incorrect SNP calls resulting from repetitive genomic regions, and thus outperforms the original ML algorithm by achieving much higher genotyping accuracy. Our algorithm is therefore very useful for accurate de novo SNP genotyping in the non-model organisms without a reference genome.This article was reviewed by Dr. Richard Durbin, Dr. Liliana Florea (nominated by Dr. Steven Salzberg) and Dr. Arcady Mushegian.
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